• Title, Summary, Keyword: nitrite scavenging effect

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Nitrite scavenging activity and protective effect of the Puerariae Radix and green tea extract on lead acetate and cadmium-induced liver damage in mice (갈근 및 녹차추출물의 아질산염 소거작용 및 간독성 보호효과)

  • Yun, I-Ran;Choi, You-Jeong;Heo, Jung-Ho;Choi, Chul-Yung;Seoung, Tae-Jong;Kim, Yun-Geun;Kim, Jong-Shu
    • Korean Journal of Veterinary Service
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    • v.33 no.3
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    • pp.275-285
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    • 2010
  • This study was designed to evaluated the nitrite scavenging activity and protective effect of the Puerariae Radix and green tea extract on lead acetate and cadmium-induced liver damage in mice. The quantitative analytical method for major antioxidants, isoflavones, puerarin, catechine and caffeine in galgun (Puerariae Radix) and green tera extract were established by HPLC. Contents of isoflavones, such as daidzin, genistin, daidzein and genistein were 4.23g/100g, 0.13g/100g, 0.07g/100g, and 0.03g/100g, and puerarin contents was 8.99g/100g, respectively. The total catechins and caffeine contents of green tea extract were 49.24g/100g and 6.53g/100g. The nitrite scavenging ability of galgun extract (pH 1.2, 100mg/ml) was 98.07% and it was higher than those of other extracts. It was the highest at the pH 1.2 and more than 64% in 25~100mg green tea extract, and was dependents on pH and concentration of the samples. The hepatoprotective effects of an aqueous extract from the root of gal gun and standard puerarin were evaluated against lead acetate and cadmium-induced liver damage in mice. Galgun extract and standard at a dose of 100mg/kg and 10mg/kg, 50mg/kg were administered orally once daily for successive 5 days and then a lead acetate and cadmium were orally at 3 hrs after the every day administration of galgun. The substantially elevated serum enzymatic activities of alanine and aspartate aminotransferase were due to lead acetate and cadmium treatment was dose dependently restored to the near normal level. In addition, galgun extract also significantly prevented the elevation of hepatic malon-dialdehyde formation in the liver of lead acetate and cadmium intoxicated mice in a dose-dependent manner. The results of this study clearly indicated that green tea and galgun extracts had nitrite scavenging ability and galgun extract had potent hepato-protective effects against lead acetate and cadmium-induced hepatic damage in mice and standard puerarin was also showed similar to the results of the galgun extracts.

Effect of Green Tea(Camellia sinensins) and Maesil(Prunus mime) Extracts on Endogenous Formation of N-Nitrosodimethylamine (녹차 및 매실 추출물이 N-Nitrosodimethylamine의 생성에 미치는 영향)

  • 성낙주;최선영;정미자;신정혜;김행자
    • The Korean Journal of Food And Nutrition
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    • v.15 no.1
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    • pp.16-22
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    • 2002
  • The effects of nitrite scavenging. electron donating and N-nitrosodimethylamine(NDMA) formation in vitro arid green tea(Camellia sinensis) and Maesil(Prunus mume) were studied. The green tea and Maesil extracts were tested for their nitrite-scavenging effect under the different pH conditions such as pH 1.2, 4.2 aud 6.0. The effects of nitrite-scavenging in all concentrations were diminished in the alkali condition, while its effects in the acidic condition of pH 1.2 were reached of more than 99.0% by adding above 0.5ml of green tea extract. And also, nitrite-scavenging effect by adding 3ml of Maesil extract was about 77.0%. The electron donating ability(EDA) of green tea and Maesil extracts was 70.6%, 75.1%, respectively. The formation of NDMA was very effectiveness which was inhibited 82.1%, 73.2% at reaction mixture of pH 2.5 adding 3ml of above extracts. respectively . The ground CW. TW1 and TW2 (refer to Table 1) diets were incubated with 10m1 simulated saliva and 40m1 gastric juice at 37$^{\circ}C$ for 2hrs. NDMA formation was inhibited at all levels of green tea and Measil extracts.

Evaluations on Antioxidant Effect of Methanol Extract from Immature Cotton Boll (미성숙 목화다래 메탄올 추출물의 항산화 효능 평가)

  • Park, Hee-Jeong;Lee, Ki-Young
    • Korean Journal of Plant Resources
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    • v.26 no.4
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    • pp.426-432
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    • 2013
  • The results of the content of total polyphenol and flavonoid, DPPH (1-1-diphenyl-2-picryl-hydrazyl) radical scavenging activity, ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] radical scavenging activity, nitrite scavenging activity and SOD-like activity of methanol extracts from immature cotton boll are follows. The contents of total polyphenol and flavonoid compound were higher in small size cotton boll, and DPPH and ABTS radical scavenging activity also showed a relatively high activity in the small size. These results indicate that there is a correlation between phenol content and DPPH radical scavenging, ABTS radical scavenging. The test concentrations of immature cotton boll extract for measuring DPPH and ABTS radical scavenging activities were set as 1.25, 2.5, 5, 10 and 20 mg/ml. Immature cotton boll has high radical scavenging activity at the concentration of 1.25~20 mg/ml and the result showed a tendency to increase in a concentration-dependent. The nitrite scavenging activity showed high activity in the pH 1.2, and the result in the pH 4.2 showed progressively less active, and in the pH 6.0 near neutral was confirmed that does not affect the nitrite scavenging. In addition, SOD-like activity showed somewhat lower activity compared with ascorbic acid, but tended to be higher when compared with the results of the other natural substances. Through this experiment, we confirmed that immature cotton boll was excellent antioxidant activity. Therefore, it is demonstrated that the cotton suggest the possibility of development of new material for cosmetic product or functional food in the future, and is expected to make a greater usability.

Effect of Enzyme Treatment on Functional Properties of Nectarine Beverage (효소처리에 의한 천도복숭아 음료의 기능성 증진)

  • Youn, Sun-Joo;Lee, Eun-Tag;Cho, Jun-Gu;Kim, Duk-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.9
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    • pp.1379-1383
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    • 2010
  • Nectarine beverage treated with cellulase and pectinase enzymes was measured for mineral contents, total flavonoids, and free amino acids and DPPH radical scavenging effect, nitrite scavenging effect. Total flavonoid contents of the no treatment, treated with pectinase, with cellulase, and with both measured 0.146 mg/mL, 0.167 mg/mL, 0.148 mg/mL and 0.171 mg/mL, respectively. DPPH was measured as 13.42% with no treatment and more than 28.98% with enzyme treatments. Nitrite scavenging effect with no treatment was 79% at pH 1.2. Whereas, it was measured above 90% while treated with enzymes at pH 1.2. And also, the nitrite scavenging effect was slightly higher at pH 3.0, pH 4.0 and pH 6.0 than no treatment. Results of free amino acids analysis revealed that, aspartic acid, serine, alanine, $\gamma$-aminobutylic acid, and glutamic acid were present with the amount ranging from 86.71% to 94.14% from total detected free amino acids. Ornithine and taurine were also observed from the beverages. The mineral contents, nitrogen element (T-N) of enzyme treatment of nectarine beverages were measured slightly higher than T-N of no treatment, however, the $P_2O_5$ was similar. Moreover, CaO, MgO and $K_2O$ in the beverages were measured above 45 mg/L, 85 mg/L and 2,133 mg/L, respectively.

Effect of Hot Water Extract of Undaria pinnatifida on the Activities of Antioxidant and Nitrite Scavenging (미역 열수 추출물의 항산화 및 아질산 제거효과)

  • Kim, Yoon-Soo;Nam, Hyung-Gun;Shin, Hyun-Jae;Na, Myung-Soon;Kim, Mi-Hye;Lee, Chul-Won;Kim, Jong-Soo;Piao, Yu Lan;Cha, Wol-Suk
    • KSBB Journal
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    • v.26 no.2
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    • pp.151-156
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    • 2011
  • For the development of functional food and cosmetics using the hot water extract of Undaria pinnatifida, the concentrations of vitamin, amino acid and element and activities of antioxidant and nitrite scavenging were investigated. The results are shown as follows: Vitamin C and E concentrations were 0.301 and 0.11 mg/100 g, respectively. Mineral concentrations were an order of Ca > Mg > K > Fe. The concentrations of total amino acids were an order of Glu > Ala > Val > Leu > Gly > Pro. Total phenol concentration and DPPH radical scavenging activity were increased with the increase of the concentration of extract. Especially, when the extract concentrationwas increased from 1.0 to 10.0 mg/mL, the total phenol concentration was increased from 0.043 to 0.125 OD 725 nm. DPPH radical scavenging activity at 50 mg/mL was 70.1%. The antioxidant activity of extract was stable in range of 80 to $140^{\circ}C$ and pH 3-9. The nitrite scavenging activity was increased with the decreaseof pH and the increase of it's extract concentration. Especially, it was 83.4% at 50 mg/mL (pH 1.2). These results showed that the hot water extract of U. pinnatifida can be applied to functional food and cosmetics.

Electron Donating Ability and Nitrite Scavenging Activity of Materials in a Traditional One-dish Meal (Bibimbab) (한국 전통 한 그릇 음식(비빔밥) 및 그 재료들의 항산화성과 아질산염 소거능)

  • Kim Up Sik;Yoon Hae Kyung;Koo Seung Ja
    • Korean journal of food and cookery science
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    • v.20 no.6
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    • pp.677-683
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    • 2004
  • The electron donating ability and nitrite scavenging activity were measured in ethanol extracts from the raw, simple cooked and seasoned materials of Bibimbab (mixed rice), a Korean traditional one-dish meal. The ethanol extracts of raw fragrant edible wild aster exhibited the highest electron donating ability $(80.28\%)$, as measured by the DPPH method. When Bibimbab was compared with Kimbab and hamburgers, which are popular food, it showed the highest antioxidative activity by the DPPH method. The nitrite scavenging ability was measured in an environment similar to in human digestive organs, i.e. pH $1.2\~6.0$. Generally, the antioxidative activity decreased in the sequence pH 1.2, 3.0 and then 6.0. The simple cooked materials, with the exception of fragrant edible wild aster, increased the antioxidative potential. The seasoned materials also had a tendency to increase the antioxidative potential. In conclusion, the ethanol extract of Bibimbab showed the highest nitrite scavenging activity. Therefore, the nutritional superiority of Bibimbab was confirmed through its electron donating ability, as measured by the DPPH method, and nitrite scavenging effect.

Antioxidant Activity and Nitrite Scavenging Ability of Olive Leaf (Olea europaea L.) Fractions (올리브 잎 분획물의 항산화기능과 아질산염 소거능력 평가)

  • Choi, Nam-Young;Lee, Jae-Hwan;Shin, Han-Seung
    • Korean Journal of Food Science and Technology
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    • v.40 no.3
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    • pp.257-264
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    • 2008
  • In this study, the antioxidant activities and nitrite scavenging abilities of olive leaf fractions acquired from plants cultivated in Australia (Olea europaea L. var. Picual) and Spain (Olea europaea L. var. Hojiblanca) were evaluated. Oleuropein was found to be the major phenolic compound in the leaves, with the butanol fractions presenting the highest contents. Antioxidant activity was evaluated in terms of superoxide dismutase (SOD)-like activity, 1,1-diphenyl-2-picryl hydroxyl (DPPH) radical scavenging activity, and the inhibitory effect on the auto-oxidation rate of linoleic acid. The SOD-like activities of the olive leaf extracts ranged from 0 to 36.8%. DPPH radical scavenging activity was highest in the ethanol extract of the Australian cultivated olive leaves. Finally, the chloroform fractions of the extracts showed inhibitory effects on the auto-oxidation rate of linoleic acid as well as nitrite scavenging ability.

Biofunctional Characteristics of the Water Soluble Browning Reaction Products Isolated from Korean Red Ginseng-Study on the Antimutagenic and Nitrite Scavenging Activities (홍삼으로부터 분리한 수용성 갈변물질의 생리기능적 특성- 돌연변이원성 및 아질산염 소거능을 중심으로)

  • 이종원;배영일;심기환
    • Journal of Ginseng Research
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    • v.25 no.3
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    • pp.118-121
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    • 2001
  • The purpose of this study was to investigate the biofunctional characteristics of water soluble browning reaction products(WS-BRPs) isolated from Korean red ginseng. The antimutagenic effect of WS-BRPs(L, S-1 and S-2 fraction) showed 23∼61% inhibition rates against the indirect mutagen, imidazol quinoline(IQ) induced mutagenicity. Especially, L fraction exhibited a little higher mutagenicity than those of other fractions on Salmonella typhimurium TA 98 and TA 100 at a concentration of 400 $\mu\textrm{g}$/m/, respectively, and it showed about 16∼39% inhibition rates on the nitrite scavenging. L fraction exhibited a little higher nitrite scavenging than those of S-1 and S-2 fractions.

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Some Functional Properties of Extracts from Leaf and Fruit Stalk of Hovenia dulcis (헛개나무 잎과 과병추출물의 몇가지 가능성)

  • Jeong, Chang-Ho;Shim, Ki-Hwan
    • Korean Journal of Food Preservation
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    • v.7 no.3
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    • pp.291-296
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    • 2000
  • The present study was conducted to investigate antioxidant, nitrite scavenging and alcohol degradation effects of extracts from leaf and fruit stalk of Hovenia dulcis. Yields of various solvent extracts for Hovenia dulcis leaf and fruit stalk of were higher in water and methanol extract layer, respectively. Ethanol extracts of Hovenia dulcis leaf and fruit stalk of were fractionated with different solvents such as hexane, chloroform, ethyl acetate, butanol and water, yields of water fraction were highest. In the solvent extracts using methanol, ethanol, hexane, chloroform and water, ethanol extracts showed the most effective antioxidant and nitrite-scavenging effects. Ethanol extracts from Hovenia dulcis leaf and fruit stalk of were fractionated, the most natioxidant and nitrite-scavenging effects were ethyl acetate fraction. Alcohol degradation effects from different parts of Hovenia dulcis showed higher leaf and fruit stalk than xylem, branch and seed. Alcohol degradation effects from leaf and fruit stalk increased as time passed.

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Antioxidative Activity and Nitrite Scavenging Ability of Ethyl Acetate Extract from Acanthopanax sessiliflorus (오가피 메탄올 추출물의 항산화 효과 및 아질산염 소거작용)

  • Lim, Jin-A;Yun, Bo-Won;Kang, Jeong-Il;Baek, Seung-Hwa
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.4
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    • pp.955-960
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    • 2007
  • Efficacy of antioxidative activity and nitrite scavenging ability of methanol extract from Acanthopanax sessiliflorus was investigated. Electron-donating ability of extract at $RC_{50}$ was 29.93 ${\mu}g/mL$. After addition of 0.96 mg/mL extract, autooxidation of pyrogallol decreased to 22.85% by superoxide dismutase-like activity. In antioxidative activity of extract against linoleic acid during incubation times of 24, 48, 96 hours at $40^{\circ}C$, lipid peroxidation values significantly decreased by 48.89%, 45.0%, 46.34% with addition of 0.2 mg/mL, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and values revealed $410.25{\pm}4.74$ GAE ${\mu}g/mg$ of extract. Nitrite scavenging ability showed the most remarkable effect at pH 1.2, exhibited to 88.3% by addition of 0.2 mg/mL. These results suggest that methanol extract from A. sessiliflorus can be used as bioactive and functional material.