• Title, Summary, Keyword: nitrite scavenging effect

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Electron Donating Abilities, Nitrite Scavenging Effects and Antimicrobial Activities of Smilax china Leaf (청미래 덩굴잎(Smilax china) 추출물의 전자공여능, 아질산염 소거능 및 항균효과)

  • 김철암;박정륭;김정희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.4
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    • pp.621-625
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    • 2004
  • Electron donating abilities, nitrite scavenging effects and antimicrobial activities of various fractions obtained from ethanol extract of Smilax china were examined. Among the fractions investigated, the highest electron donating ability was determined with ethyl acetate fraction showing about 81.0% when reacted for 10 min. However, the lowest ability was found from chloroform fraction. Ethyl acetate and butanol fractions also showed very high nitrite scavenging activity at all concentrations tested. All the fractions revealed antimicrobial activity against Listeria monocytogenes and Staphylococcus aureus, Gram (+) bacteria, at both 2.5% and 5.0% concentrations. However, no antimicrobial activity was observed on Escherichia coli O157:H7 and Salmonella Typhimurium, Gram (-) bacteria, at 2.5%, but very low activity was detected by 5.0% concentration of ethanol extract, ethyl acetate and water fractions.

The Electron Donating Ability, Nitrite Scavenging Ability and NDMA Formation Effect of Solvent Extracts from Yuza (Citrus junos SIEB ex TANAKA) (유자 용매추출물의 전자 공여능, 아질산염 소거능 및 NDMA 생성 억제능)

  • Lee Soo-Jung;Choi Sun-Young;Shin Jung-Hye;Seo Jong-Kwon;Lim Hyun-Cheol;Sung Nak-Ju
    • Journal of Food Hygiene and Safety
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    • v.20 no.4
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    • pp.237-243
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    • 2005
  • Methanol, ether, ethylacetate and water extracts from flesh and peel of yuna (Citrus junos) were investigated to analyze their ability of electron donating, nitrite scavenging and nitrosodimethylamine (NDMA) formation in model system. The electron donating ability of ynza flesh extract, when it added at 10 mg/ml or over, were more an $50\%$, except ether extract. The high electron donating ability ($99.2{\pm}0.37\%$) was observed in the methanol extract and its effect was similar to BHA and ascorbic acid, when 40 mg/ml of methanol extract was added in reaction solution. Nitrite scavenging ability of all extracts ken yuza was increased in proportion to sample concentration and more than $40\%$ when 1 mg/ml sample was added, under pH 1.2. Also nitrite scavenging ability was higher in peel extracts than flesh extracts of yuza. Inhibition ratio of NDMA formation from flesh methanol extract of yuza was $31.7{\pm}1.25\%$, when 40 mg/ml added under pH 1.2. In all samples, inhibition effects were lower than $20\%$, at pH 4.2 and 6.0.

Characteristics of Nitrite Scavenger Derived from Seeds of Cassia tora L. (결명자 추출물의 아질산염 소거인자의 특성)

  • Park, Yeung-Beom;Lee, Tae-Gee;Kim, Oi-Kyung;Do, Jeong-Ryong;Yeo, Saeng-Gyu;Park, Yeung-Ho;Kim, Seon-Bong
    • Korean Journal of Food Science and Technology
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    • v.27 no.1
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    • pp.124-128
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    • 1995
  • The present study was conducted to elucidate nitrite scavenger derived from seeds of Cassia tora L. Methanolic extract of Cassia tora L was refractionated into ethyl ether, chloroform, ethyl acetate and water farction, and nitrite scavenging abilities of these fractions were investigated. Among these fractions, ethyl acetate fraction had the strongest nitrite scavenging ability(97%/2 mg). Therefore, to further investigate nitrite scavenger, ethyl acetate fraction was fractionated by silica gel column chromatography with a chloroform-methanol($10:0{\sim}0:10$) and then compound A and B were isolated. Compound A had a stronger nitrite-scavenging effect than compound B. Therefore the further separation of compound A was carried out by HPLC(32% acetonitrile. 1 ml/min) using ${\mu}Bondapak$C18 column$(3.9{\times}300 mm)$ and finally compound A-1 was obtained from compound A. Compound A-1 had by far nitrite-scavenging ability as compared with that of ascorbic acid. Compound A-1 was identified as $nor-rubrofusarin-6-{\beta}-mono-D-glucoside$ from the profiles of UV, IR and $^1H-NMR$.

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Inhibition of N-nitrosodimethylamine Formation of Extracts from Citrus Seeds (감귤류 종자 추출물의 N-nitrosodimethylamine 생성 억제에 관한 연구)

  • 오혁수;안용석;나임순;오명철;오창경;김수현
    • Korean journal of food and cookery science
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    • v.19 no.5
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    • pp.640-646
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    • 2003
  • The effect of inhibition on N-nitrosodimethylamine (NDMA) formation by methanol extracts from 6 kinds of citrus seed (Citrus sunki, Citrus natsudaidai, Citrus sulcata, Citrus tangerina, Citrus grandis and Citrus obovoidea) were investigated. The contents of moisture, crude protein, crude fat, carbohydrate, ash, flavonoid and total phenol in citrus seeds were 4∼6, 11∼15, 32∼46, 22∼45, 2∼4%, 12∼24mg% and 53∼133mg%, respectively. The solid contents and yield of citrus seed extracts were 0.8∼1.0 and 0.7 ∼ 1.1%, respectively. Nitrite-scavenging activity by methanol extracts from citrus seeds increased with increasing extract dosage. Furthermore, the nitrite scavenging activity was pH dependent being the highest at pH 1.2 (42.7∼96.9%) and the lowest at pH 6.0 (19.9-62.6%). Scavenging effects of nitrite by reaction time showed high effects under 3hr reaction time. The inhibition effect of NDMA formation by the citrus seed was strengthened as the amount of extract increased. The inhibition rate of methanol extracts from citrus seeds on NDMA formation showed 1.2∼39.8%, 21.3∼60.1% and 47.4∼94.0%, according to add 1, 3 and 5$m\ell$, respectively. Therefore, the inhibition effect of NDMA formation by the citrus seed was strengthened as the concentration of extract increased.

Activities of Antioxidation and Alcohol Dehydrogenase Inhibition of Methanol Extracts from Some Medicinal Herbs (약용식물 추출물에 대한 항산화성과 알코올 탈수소효소 저해성 연구)

  • 문지숙;김선재;박윤미;황인식;김의형;박정욱;박인배;김상욱;강성국
    • Korean Journal of Food Preservation
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    • v.11 no.2
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    • pp.201-206
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    • 2004
  • The activities of antioxidation and alcohol dehydrogenase in hibitionin methanol extracts of thirty two medical herbs were tested using the method of DPPH activity, nitrite scavenging effect and alcohol dehydrogenase assay in vitro. In DPPH method, Eugenia caryophyllata, Thea sinensis, Paeonia suffruticosa, Alnus japonica showed over 90 % of free radical scavenging activities. The nitrite scavenging ability appeared Zanthoxylum bungeanum, Alnus japonica, Thea sinensis, Hovenia dulcis(cortex) and Illicium verum showed the high value. In connection with in vivo alcohol metabolism, thirteen medicinal herbs were screened for inhibition. As a reasult, we found significant inhibition of ADH by methanolic extracts of Glycyrrhiza uralensis, Pueraria thunbergiana(radix), Alnus japonica. These results indicate that the antioxidative effect was strongly related with alcohol dehydrogenase inhibitor; Thea sinensis and Alnus japonica.

Identification of the Antioxidative Function of Metallothionein by Oxidation of NADPH and Production of Nitrite (NADPH의 산화반응과 아질산 생성반응에 의한 Metallothionein 의 항산화적 기능 확인)

  • Kim Kwan-Chun;Kim Joon-Tae;Kim Hee-Joung
    • Journal of environmental and Sanitary engineering
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    • v.21 no.1
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    • pp.52-57
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    • 2006
  • Metallothioneins(MTs) belong to the class of low molecular weight proteins. Recently, it has been suggested that MTs may playa direct role in cellular defense against oxidative stress by functioning as antioxidants. Oxidative damage to different cellular components makes a major contribution to many pathogenenesses. Several studies have demonstrated that MT is able to quench a wide range of reactive oxygen species at a higher efficiency than other well known antioxidants such as superoxide dismutate(SOD). The present study was designed to evaluate the effect of MT on the activities of the reactive oxygen species removal system. MT showed the scavenging of superoxide in the SOD assay system in the presence or absence of SOD. When MT was added to nicotinamide adenine dinucleotide phosphate(NADPH) oxidation system in presence of fixed amount of SOD increase the breakdown rate of superoxide. When MT was added to the system that form nitrite from hydroxylammonium chloride, the formation of nitrite was inhibit. We concluded that the function of MT as antioxidant might have an effect on the level of superoxide scavenging.

Chemical Compositions and Physiological Activities of Doraji(Platycodon grandiflorum) (장생 도라지의 화학성분과 생리활성)

  • 손미예;서종권;김행자;성낙주
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.4
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    • pp.717-720
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    • 2001
  • Chemical compositions and physiological activities of Platycodon grandiflorum roots grown for 4 and 24 years were investigated. Chemical compositions of P. grandiflorum roots grown for 24 years were moisture 82.7%, crude protein 1.6%, crude lipid 2.1% crude ash 0.7%. total sugar 6.0% total dietary fiber 2.3% crude saponin 3.4mg% and ascorbic acid 3.0 mg%. Contents of each components in 24 years old roots were higher than that in 4 years old roots and the most abundant mineral was potassium being 879.9mg/kg. Physiological activities of solvent extract from P. grandiflorum roots grown for 4 and 24 years were high in order of ethanol methanol and water extract. The electron-donating abilities and nitrite-scavenging effect of solvent extract of 24 years were high in order of ethanol methanol and water extract. The electron-donating abilities and nitrite-scavenging effect of solvent extract of 24 years old roots were higher than those of 4 years of old roots in the range of 10~40% respectively.

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Effects of Mechanochemical Pretreatment on the Extraction of Functional Materials from Houttuynia cordata

  • Park, Keum-Joo;Song, Won-Seob;Islam, Mohammad Nazrul;Lee, Jong-Ill;Kim, Yang-Soo
    • Korean Journal of Plant Resources
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    • v.24 no.3
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    • pp.309-313
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    • 2011
  • Houttuynia cordata is used as a raw material of oriental medicine to acquire antioxidant and anti cancer effect and to cure a heart disease. Mechanochemical technology not only reduces the size of the object but also changes the chemical properties of the object. Extraction of functional materials from the Houttuynia cordata after grinding as a pretreatment using the mechanochemical technology was conducted in this study to investigate the effect of grinding on the yields and antioxidant activities of the extract. Houttuynia cordata was ground by the planetary ball mill and the morphology was analyzed by SEM. Yields of functional materials were increased from 6.2 g in the sample without grinding to 7.0, 7.8, 8.8 g after grinding of 30 minutes, 1 hour and 2 hours, respectively. Nitrite scavenging abilities were increased from 57-77% to 69-86% as a result of mechanochemical pretreatment. Also, DPPH scavenging abilities for the methanol extraction were increased from 10.01-40.29% to 11.01-49.29% as a result of mechanochemical grinding.

Effect of Garlic (Allium sativum L.) Extracts on Formation of N-Nitrosodimethylamine (마늘 추출물이 N-Nitrosodimethylamine의 생성에 미치는 영향)

  • Choi, Sun-Young;Kim, Hyung-Sik;Lee, Soo-Jung;Shon, Mi-Yae;Shin, Jung-Hye;Sung, Nak-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.6
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    • pp.677-682
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    • 2006
  • This study was conducted to investigate the effect of garlic extracts on electron donating ability nitrite-scavenging activity and N-nitrosodimethylamine (NDMA) formation. Content of total phenolic compounds was the highest in ethyl acetate extract. Electron donating ability was significantly increased with increasing sample concentration, as $88.0{\pm}1.53%$ from ethyl acetate extract and $77.5{\pm}0.8%$ from butanol extract in concentration of 10 mg/mL. Butanol extract from garlic with highest nitrite scavenging activity and inhibition effect of NDMA formation exhibited $96.9{\pm}0.75%$ and $68.6{\pm}1.15%$ in concentration of 10 mg/mL, respectively. The butanol extract was fractionated into 5 fractions using prep-LC. Nitrite-scavenging activity and inhibition effect of NDMA formation were excellent in fraction III, which further fractioned into 3 subfractions (III-a, III-b and III-c) using prep-LC. Of the 3 subfractions, its activity was the highest in the subfraction III-c. Therefore butanol extract of garlic was suggested to be effective inhibitor of NDMA formation.

In Vitro antioxidant effect of ethanol extract from Pennisetum purpureum (Napier grass (Penninsetum purpureum) 에탄올 추출물의 in vitro 항산화 효과)

  • Kwon, Young Ji;Kim, Dong Chung
    • Journal of Applied Biological Chemistry
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    • v.62 no.2
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    • pp.167-172
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    • 2019
  • In vitro antioxidant effect of 50% ethanol extract from Napier grass (Penninsetum purpureum) was investigated. The yield and polyphenol content of the Napier grass extract were $6.3{\pm}0.35%$ and $79.6{\pm}3.65{\mu}g$ gallic acid equivalents/mg-extract, respectively. Antioxidant ability of the Napier grass extract such as fee radical and cation radical scavenging activities, reducing power, nitrite scavenging activity, and lipid peroxidation inhibitory activity proportionally increased as concentration of the extract increased. $EC_{50}$ values of the Napier grass extract for free radical scavenging, cation radical scavenging, reducing power, and nitrite scavenging were 1,930.0, 350.0, 840.0, and $1,470.0{\mu}g/mL$, respectively. In the presence of $85.0{\mu}g/mL$ of the Napier grass extract, lipid peroxidation was potently inhibited by 74.6%.