• Title/Summary/Keyword: non-marker

Search Result 517, Processing Time 0.033 seconds

Standardization and Usefulness of ISAG Microsatellite Markers for Individual Identification and Parentage Verification in Horse Breeds (말에서 개체식별 및 친자확인을 위한 ISAG Microsatellite Marker의 유용성 및 표준화)

  • Kwon, Do-Yeon;Cho, Gil-Jae
    • Journal of Veterinary Clinics
    • /
    • v.26 no.3
    • /
    • pp.220-225
    • /
    • 2009
  • The present study demonstrates a new approach that enables effective horse parentage testing using 22 ISAG microsatellite markers involving 6 heads of Thoroughbred horse(TB) and non-TB. In the comparison allele distribution between these horses, the alleles found in the TB were numerously detected in the non-TB. As results, we confirmed that these ISAG microsatellite markers might apply the pedigree registration of Korean native horse(Jeju horse).

Non-Discourse Marker Uses of So in EFL Writings: Functional Variability among Asian Learners

  • Sato, Shie
    • Asia Pacific Journal of Corpus Research
    • /
    • v.1 no.2
    • /
    • pp.27-39
    • /
    • 2020
  • This paper examines the frequency and distribution of the so-called "non-discourse marker functions" of so in essay writings produced by 200 L1 English speakers and 1,300 EFL learners in China, Japan, Korea, and Taiwan. Based on the data drawn from the International Corpus Network of Asian Learners of English, this study compares EFL learners and L1 English speakers' uses of so, identifying four grammatical uses, as (1) an adverb, (2) part of a fixed phrase, (3) a pro-form, and (4) a conjunction phrase specifying purpose. This study aims to show the wide variability among EFL learners with different L1s, identifying the tendency of usage both common among and specific to the sub-groups of EFL learners. The findings suggest that the learners demonstrate patterns distinctively different from those of L1 English speakers, indicating an underuse of so as a marker expressing "purpose" and an overuse as part of fixed phrases. Compared to L1 English speakers, the learners also tend to overuse so in the discourse marker functions, regardless of their L1s. The study proposes pedagogical implications focusing on discourse flow and diachronic aspects of so in order to understand its multifunctionality, although the latter is primarily suggested for advanced learners.

The study for improve a method of Marker auto- identification (마커 자동 인식 향상 방법에 관한 연구)

  • Lee, Hyun-Seob
    • Korean Journal of Applied Biomechanics
    • /
    • v.13 no.1
    • /
    • pp.23-38
    • /
    • 2003
  • The purpose of this study is to develop an improved marker auto-identification algorithm for reduce of data processing time through improve the efficiency of noise elimination and marker separation. The maker auto-identification algorithm was programming named KUMAS used Delphi language. For the study, various experiments were conducted for the verification of KUMAS. and compared two systems of established with the KUMAS. Four different motions - cycling, gait, rotation, and pendulum -, were selected and tested. Motions were filmed 30Hz frames rate per second. ${\chi}^2$ used for statistical analysis. Significant level were ${\alpha}=.05$. The test results were as follow. 1. Increased the success ratio of marker auto-identification. 2. The efficiency of marker auto-identification was remarkably improved through marker separation, noise elimination. 3. The marker auto-identification ability was improved in 2D-image plane include the 3D motion. 4. Significant different were found between KUMAS and B-SYS(established system) with non-input the artificial noise frames, input the artificial noise frames and total frames.

Measurement of Dynamic Characteristics on Structure using Non-marker Vision-based Displacement Measurement System (비마커 영상기반 변위계측 시스템을 이용한 구조물의 동특성 측정)

  • Choi, Insub;Kim, JunHee
    • Journal of the Computational Structural Engineering Institute of Korea
    • /
    • v.29 no.4
    • /
    • pp.301-308
    • /
    • 2016
  • In this study, a novel method referred as non-marker vision-based displacement measuring system(NVDMS) was introduced in order to measure the displacement of structure. There are two distinct differences between proposed NVDMS and existing vision-based displacement measuring system(VDMS). First, the NVDMS extracts the pixel coordinates of the structure using a feature point not a marker. Second, in the NVDMS, the scaling factor in order to convert the coordinates of a feature points from pixel value to physical value can be calculated by using the external conditions between the camera and the structure, which are distance, angle, and focal length, while the scaling factor for VDMS can be calculated by using the geometry of marker. The free vibration test using the three-stories scale model was conducted in order to analyze the reliability of the displacement data obtained from the NVDMS by comparing the reference data obtained from laser displacement sensor(LDS), and the measurement of dynamic characteristics was proceed using the displacement data. The NVDMS can accurately measure the dynamic displacement of the structure without the marker, and the high reliability of the dynamic characteristics obtained from the NVDMS are secured.

Marker Genes for in Vitro Selection of Transgenic Plants

  • Brasileiro, Ana C.M.;Aragao, Francisco J.L.
    • Journal of Plant Biotechnology
    • /
    • v.3 no.3
    • /
    • pp.113-121
    • /
    • 2001
  • The use of a marker gene in a transformation process aims to give a selective advantage to the transformed cells, allowing them to grow faster and better, and to kill the non-transformed cells. In general, the selective gene is introduced into plant genome along with the genes of interest. In some cases, the marker gene can be the gene of interest that will confer an agronomic characteristic, such as herbicide resistance. In this review we list and discuss the use of the most common selective marker genes on plant transformation and the effects of their respective selective agents. These genes could be divided in categories according their mode of action: genes that confer resistance to antibiotics and herbicides; and genes for positive selection. The contention of the marker gene flow through chloroplast transformation is further discussed. Moreover, strategies to recover marker-free transgenic plants, involving multi-auto-transformation (MAT), co-transformation, site specific recombination and intragenomic relocation of transgenes through transposable elements, are also reviewed.

  • PDF

Evaluation of the Usefulness of the Self-developed Kw-infrared Reflective Marker in Non-coplanar Treatment (비동일면 치료 시 자체 제작한 Kw-infrared Reflective Marker의 유용성 평가)

  • Kwon, Dong-Yeol;Ahn, Jong-Ho;Park, Young-Hwan;Song, Ki-Won
    • The Journal of Korean Society for Radiation Therapy
    • /
    • v.22 no.1
    • /
    • pp.25-32
    • /
    • 2010
  • Purpose: In radiotherapy that takes into account respiration using a RPM (Real time Position Management, Varian, USA) system, which can treat in consideration of the movement of tumor, infrared reflective markers supplied by manufacturers cannot obtain respiratory signal if the couch rotates at a certain angle or larger. In order to solve this problem, the author developed the 3D infrared reflective marker named 'Kw-marker' that can obtain respiratory signal at any angle, and evaluate its usefulness. Materials and Methods: In order to measure the stability of respiratory signal, we put the infrared reflective marker on the 3D moving phantom that can reproduce respiratory movement and acquired respiratory signal for 3 minutes under each of 3 conditions (A: $couch\;0^{\circ}$, a manufacturer's infrared reflective marker B: $couch\;0^{\circ}$, Kw-marker C: $couch\;90^{\circ}$, Kw-marker). By analyzing the respiratory signal using a breath analysis program (Labview Ver. 7.0), we obtained the peak value, valley value, standard deviation, variation value, and amplitude value. In order to examine the rotation error and moving range of the target, we placed a B.B phantom on the 3D moving phantom, and obtained images at a couch angle of $0^{\circ}$ and $90^{\circ}$ using OBI, and then acquired the X, Y and Z values (mm) of the ball bearing at the center of the B.B phantom. Results: According to the results of analyzing the respiratory signal, the standard deviation at the peak value was A: 0.002, B: 0.002 and C: 0.003, and the stability of respiration for amplitude was A: 0.15%, B: 0.14% and C:0.13%, showing that we could get respiratory signal stably by using the Kw-marker. When the couch rotated $couch\;90^{\circ}$, the mean rotation error of the ball bearing, namely, the target was X: -1.25 mm, Y: -0.45 mm and Z: +0.1 mm, which were within 1.3 mm on the average in all directions, and the difference in the moving range of the target was within 0.3 mm. Conclusion: When we obtained respiratory signal using the Kw-marker in non-coplanar treatment where the couch rotated, we could acquire respiratory signal stably and the Kw-marker was effective enough to substitute for the manufacturer's infrared reflective marker. When the rotation error and moving range of the target were measured, there was little difference, indicating that the displacement of the reflector movement in couch rotation is the cause of change in the scale and amplitude of respiratory signal. If the converted value of amplitude height according to couch angle is studied further and applied, it may be possible to perform non-coplanar phase-based gating treatment.

  • PDF

Identification of Hanwoo Meat by DNA Analysis (DNA 분석법에 의한 한우고기 판별)

  • Oh, Hong-Rock;Lee, Chang-Soo;Sang, Byung-Chan;Song, Kwang-Taek
    • Korean Journal of Agricultural Science
    • /
    • v.33 no.1
    • /
    • pp.1-10
    • /
    • 2006
  • This study was deal with the development of breed-specific DNA marker which is able to identify Hanwoo and European cattle breeds(Non-Hanwoo) meat. Genetic differentiation between Korean cattle(Hanwoo) and European cattle breeds was examined by Random Amplified Polymorphic DNA(RAPD) analysis. The RAPD patterns were identical among Non-Hanwoo, such as Holstein, Hereford, Aberdeen Angus, Brown Swiss, Limousin or Simmental, but the above pattern was different from that of Hanwoo. All bands detected in the Hanwoo samples were observed in Non-Hanwoo cattle samples, but one of the common bands found in samples was not detected in the Hanwoo samples. The band(1.4kb) may be useful as a marker for identifying a meat of Hanwoo from imported cattle meat. Actually, the detection of the DNA marker was tested by DNA analysis with 929 samples which were prepared from bloods of 673 Hanwoo cattles and 141 Holstein cattles, from 115 imported cattle meats. The DNA marker was absent in 644 of 673 Hanwoo cattles (96%) but present in 245 of 256 Non-Hanwoo cattles (95%). These results show that the DNA marker is effective to characterize Hanwoo and Non-Hanwoo meat by its detection. This DNA marker, however, was not useful in detecting unwanted crossbreeding between two cattle breeds, because the band pattern in hybrid cattle shows one of two band patterns in Hanwoo and Non-Hanwoo.

  • PDF

Telomerase Activity in Non-small Cell Lung Cancer (비소세포폐암에 있어서의 Telomerase 활성도)

  • 김진국;김관민
    • Journal of Chest Surgery
    • /
    • v.30 no.7
    • /
    • pp.701-707
    • /
    • 1997
  • Although many reseraches have been persued to detect the molecular tumor marker to define the cancer, ideal tumor marker which speak for the characteristics of malignancy and has high sensitivity and specificity is not known. One of the characteristics of the malignant cells is indefinite proliferative potential, in other word, immortality. The expression of telomerase and stabilization of te10meres are con omitant with the attaiunent of immortality in tumor cells; thus the measurement of telomerase activity in clinically obtained tumor samples may provide important information which would be useful as a diagnostic marker to detect immortal cancer cells. Telomerase activity was analyzed in 12 non-small cell . lung cancer cell lines and 41 primary non-small cell lung cancers with the use of a PCR-based assay. All the cell lines and the majority of tumors displayed telomerase activity, but telomerase was not detectable in most of the corresponding pathologically-normal tissues. Telomere length was not correlated with telomerase activity. The present study indicate that measurement of telomerase activity may be useful as a molecular tumor marker in non-small cell lung cancer.

  • PDF

The Study of Stable Child English Education Content Using Augmented Reality Solving the Hide of Marker (마커의 가려짐을 해결하여 증강현실을 이용한 안정적 영어 학습 컨텐츠에 대한 연구)

  • Jeon, Soo-Jin;Kim, Young-Seop
    • Journal of the Semiconductor & Display Technology
    • /
    • v.9 no.4
    • /
    • pp.99-102
    • /
    • 2010
  • In this study, the 3- dimensional (3-D) learning contents are suggested using 'Augmented Reality' instead of existing 2-dimensional (2-D) learning methods. At the present, there are some 2-D learning methods using texts, image, pictures, and videos called e-learning. However, these one-way 2-D methods have some disadvantages such as declining learner's immersion and concentration. Thus, the 3-D learning contents using 'Augmented Reality' are suggested to compensate the disadvantages. According to the development of information technology (IT), the augmented reality has many applications to the era of ubiquitous. However, there are some disadvantages when learners use these contents as following; non-augmenting by partially hiding from makers and declining concentration by patterns of the makers. In this study, the beneficial marker which can solve this non-augmenting phenomenon is suggested.

The Variable Acquisition of Discourse Marker Use in Korean American Speakers of English

  • Lee, Hi-Kyoung
    • English Language & Literature Teaching
    • /
    • v.11 no.2
    • /
    • pp.1-18
    • /
    • 2005
  • This study is a preliminary investigation of the nature of discourse marker acquisition in Korean American speakers of English. Discourse markers are of interest because they are not an aspect of language taught through formal instruction either to native or non-native speakers. Therefore, discourse marker use serves as indirect evidence of face-to-face interaction with native speakers and an indicator of integration. In this light, the present study examines the presence of discourse markers in Korean Americans. The markers chosen for analysis were you know, like, and I mean. The data consist of spontaneous speech elicited from interviews. Sociolinguistic variables such as age, sex, and generation (i.e., $1^{st}$, 1.5, $2^{nd}$) were examined. Results show that there appears to be interaction between the variables and discourse marker use. While all speakers showed variable acquisition of markers, younger, female, and 1.5 generation speakers were found to use discourse markers more than other speakers. Although discourse marker use is optional and thus not a linguistic feature that must be necessarily acquired, it is clear that use is pervasive and acquired differentially by English speakers irrespective of whether they are native or not.

  • PDF