• Parasites, Hosts and Diseases
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• v.45 no.3
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• pp.225-228
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• 2007

We observed the time gap between oocyst shedding and antibody responses in mice (3-week-old C57BL/6J females) infected with Cryptosporidium parvum. Oocyst shedding was verified by modified acid-fast staining. The individually collected mouse sera were assessed for C. parvum IgM and IgG antibodies by enzyme-linked immunosorbent assay from 5 to 25 weeks after infection. The results showed that C. parvum oocysts were shed from day 5 to 51 post-infection (PI). The IgM antibody titers to C. parvum peaked at week 5 PI, whereas the IgG antibody titers achieved maximum levels at week 25 PI. The results revealed that IgM responses to C. parvum infection occurred during the early stage of infection and overlapped with the oocyst shedding period, whereas IgG responses occurred during the late stage and was not correlated with oocyst shedding. Hence, IgM antibody detection may prove helpful for the diagnosis of acute cryptosporidiosis, and IgG antibody detection may prove effective for the detection of past infection and endemicity.

• Parasites, Hosts and Diseases
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• v.31 no.3
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• pp.201-206
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• 1993

Based on the morphologic characteristics of oocysts of the genus Isosporn, we have demonstrated that I. ohioensis is a relatively common Isosporn species in dogs which are resident in Chonbuk province, Korea. The prepatent period of 1. ohioensis was four days. The size of the unsporulated oocysts in fresh stool specimens was $22.9{\;}\times{\;}19.8{\;}\mu\textrm{m}$ (R = 1.16). The size of the contracted sporonts was $17.4{\;}\times{\;}16.3{\;}\mu\textrm{m}$ (R = 1.061). By 96 hours, sporulation is complete and the ratio of length/width was constant relatively. And the sizes of oocysts and sporocysts were $22.8{\;}\times{\;}20.5{\;}\mu\textrm{m}$ (R = 1.11) and $15.0{\;}\times{\;}10.8{\;}\mu\textrm{m}$ (R = 1.39), respectively. At this time it is most reliable for the measurements of oocyst sizes of 1. ohioensis to provide with the identifiable clues against the others. It is therefore recommended that the clinical fecal specimens suspected of isosporosis should first be incubated and aerated for 96 hours before a definitive parasitological diagnosis can be reached. These and other observations contribute to the understanding of the biological characteristics and laboratory and clinical diagnosis of isosporosis.

• Korean Journal of Veterinary Pathology
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• v.6 no.2
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• pp.73-77
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• 2002

고양이를 제외한 포유동물 및 조류는 oocyst 또는 cyst의 섭취로 감염되고 무성생식을 한다. 급성기에는 tachyzoite ($2~3{\times}5~7$\;\mu\textrm{m}$)가 여러 장기에서, 숙주 변역이 활성화된 이후인 만성기에는 100~1,000 개의 bradyzoite를 내장하며 주변에 염증반응이 없는 cyst (직경 $20~50;\mu\textrm{m}$)가 근육 및 뇌에서 관찰된다. Tachyzoite는 태반을 통해 태아 감염을 일으킨다. 고양이는 oocyst 또는 cyst의 섭취로 감염되고, 기타 동물에서 보이는 감염양식 외에 소장에서 coccidiosis양의 증식이 이루어짐으로 oocyst (Isospora 형, $10{\times}12;\mu\textrm{m}$)를 배출한다.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.4
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• pp.555-557
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• 2003

A total of 168 seven days-old Arbor Acres chicks were reared in late-autumn on 4 types of litters; sawdust, rice husk, sugarcane bagasse or wheat straw up to 49 days of age to compare the growth performance, evaluate the manureal value and Coccidial oocyst population in used litter. Sadust, rice husk, sugarcane bagasse and wheat straw did not differ statistically for live weight, feed consumption, feed conversion ratio and survivability (p>0.05). However, live weight and survivability tended to increase on sawdust. The highest moisture content of used litter was found in sugarcane bagasse followed by sawdust, rice husk and wheat straw (p<0.05). Rice husk contained the highest amount of of nitrogen, phosphurus and potassium followed by sawdust, sugarcane bagasse and wheat straw (p<0.01). Oocyst content of all treatment groups increased suddenly up to 5 weeks of age and thereafter suddenly declined up to 7 weeks of age. Litter materials did not differ at 35 and 42 days of age for oocyst content, but significantly differed at 49 days of age (p<0.01). So, the above findings reveal that sawdust may be suitable litter followed by rice husk, sugarcane bagasse and wheat straw in late-autumn in Bangladesh in respect of broiler growth performance.

• Parasites, Hosts and Diseases
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• v.46 no.2
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• pp.71-75
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• 2008

The diagnosis of cryptosporidiosis has been carried out using coprologic techniques in the Republic of Korea. However, antibody responses to Cryptosporidium have rarely been studied. Serum antibodies from HIV-positive/oocyst-positive Korean patients recognized significantly 31 and 27 kDa antigens, and HIV-negative/oocyst-positive individuals clearly reacted to 15/17 kDa antigens. Compared with oocyst-positive cases, 18.7% and 75.8% of sera from HIV-positive patients reacted to 31 and 27 kDa antigens. Only 11.1% of HIV-negative individuals reacted to 15/17 kDa. Based on these findings, serum antibody responses were different between HIV-positive and HIV-negative individuals infected with Cryptosporidium, and it is suggested that HIV-positive patients are more frequently exposed to C. parvum compared to HIV-negative individuals.

• Korean Journal of Veterinary Service
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• v.17 no.1
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• pp.44-53
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• 1994

The result that investigated aberage weekly oocyst nymbers in faeces from 16 broiler farms, Oocyst can be found in the highest during the 5 weeks age. The 3 of the 16 farms have clinical sign of the coccidiosis at the faeces collection. Anticoccidial drug sensitivity against Oocyst isolated in 2 farms to have clinical coccidiosis was tested for the drug susceptibility and resistance in order to prevention coccidiosis effectively. Maduramicin, Salynomicif Narasin Lasalocid, Monensin, Robenidine and Dicrazulir were used an anticoccidial drugs. The results ; Anticoccidial index(ACI) of the A farm were 201.50 in a chicken group treated with Dicrazulir, 194.84 with Robenidine. 172.79 with Maduramicin 6ppm 170.49 with Salynomicin, 168.02 with maduramicin 5ppm 165.77 with Lasalocid, 143.34 with Monensin and 140.63 with Narasin. ACI of the B farm were 193.40 in a chicken group treated Robenidine, 190.64 with Dicrazuril, 158.75 with Maduramic in 6ppm, 151.62 with Maduramicin 5ppm, 149.44 with Salynomicin, 143.10 with lasalocid, 138.11 with Monensin and 137.50 with Narasin. According to interpretation of ACI from Kawasae et al, the drug resistance(less than 120 of ACI) not appeared in two farms, vut the drug tolerance(120-160 of ACI) appeared in two group treated with Monesin and Nalacin in csae of A farm and in all group with treated anticoccidial drug in case of B farm.

• Parasites, Hosts and Diseases
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• v.33 no.4
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• pp.377-382
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• 1995

Three-week-old ICR SPF mice were orally inoculated with one of 5 doses ranging from $2{\;}\times{\;}10^2{\;}to{\;}2{\;}\times{\;}10^6$ oocysts of Crwptosporidium tsuris (strain MCR) per mouse. Oocyst inoculation was directly proportional to the amount of oocysts shed and was inversely proportional to the period required for peals oocyst production and to the prepatent period. Peak oocyst production occurred between fifteen and thirty-one days with a patent period from 61 to 64 days. Three days after all mice stopped shedding oocysts, they were orally challenged with a single dose of $2{\;}\times{\;}10^6$ oocysts or the same species. Marked seroconversion for IgG antibody accompanied recovery from mice inoculated with $5{\;}\times{\;}10^5$ oocysts. Mice administered with carrageenan excreted a small number of oocysts for 49.0 days on the average after challenge inoculation (ACI) and control mice for 14.2 days in a dose-independent fashion. Just before challenge infection, phagocytic activity of peritoneal macrophages ($M{\phi}$) and the number of peripheral $M{\phi}$ were dramatically decreased. Mild challenge infection implies that the immunogenicity of C. nuris (strain MCR) is very strong, despite $M{\phi}$ blocker carrageenan administration.

• Parasites, Hosts and Diseases
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• v.36 no.3
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• pp.171-182
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• 1998

A total of nine Korean native kids and two Corriedale lambs, 1-20 days old, were each inoculated per os with a single dose of 2 × 107 oocysts of Cwptospori,mum muris (strain MCR) originated from mice to elucidate the kinetics and developmental stages of the coccidium in small ruminants. Irrespective of host's age, the prepatent period for both animals ranged from 19 to 35 days (28.1 days, on the average) and the patent period 16-85 days (47.8 days), and the total oocyst outputs showed enormous differences. Infection with greater numbers of oocyst outputs was not ordinarily established by transmission experiments. Oocysts discharged from the kids retained their infectivity by the mouse titration method. The immunogenicity of the coccidium and oocyst reproduction were proven by challenge infection and administration of prednisolone acetate. respectively. All the developmental stages of the coccidium in parasitophorous vacuoles were found by transmission electron microscopy in the pits of the gastric glands of a kid inoculated with oocysts and then necropsied on day 44 postinoculation. It indicated the full course of the host-parasite relationship in kids and lambs as well as mice.

• Parasites, Hosts and Diseases
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• v.39 no.2
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• pp.201-203
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• 2001

The present study was undertaken to know the infection status of Cryptosporidium parvum among the residents of Chorwon-gun, Kangwon-do in 1993. Total 461 fecal samples were collected from the inhabitants residing in Chorwon-gun during the period of August 12 to September 14, 1993. Fecal smears were prepared by formalin-ether sedimentation, and examined after modified acid fast staining. Of the 461 fecal samples,9 (1.9%) were positive for C. parvum oocysts. The positive cases were limited to thirties (4) patients, forties (3), and sixties (2) , and no oocyst was detected in other age groups. The oocyst positive rate for male was 1.4% and that of female was 2.6%.

• Journal of Microbiology and Biotechnology
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• v.7 no.3
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• pp.189-193
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• 1997

Cryptosporidium parvum has been recognized as a significant cause of life-threatening diarrhea in Acquired ImmunoDeficiency Syndrome (AIDS) patients. Clinical diagnosis of cryptosporidial infections has been primarily based on the detection of infective stage, oocysts, in stools. Anti-Cryptosporidium oocyst monoclonal antibody (mAb), IgG2a, recognizing an antigen of 97 kDa was generated to be used for diagnosis of Cryptosporidium infection in AIDS patients using an immunofluorecence. It appeared to react with the surface antigens. Transmission electron micrographs of the infective stage of Cryptosporidium recognized by this mAb demonstrated sporolulated oocysts, which measure $4~6{\mu}m$, and sporozoites excysting from oocysts.