• Title/Summary/Keyword: organic acid

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Effect of Temperature, pH, Carbon and Nitrogen Nutritions on Mycelial Growth of Pleurotus sajor-caju (Fr.) Sing. and Pleurotus ostreatus (Fr.) $Qu{\acute{e}}l$. (여름느타리버섯과 느타리버섯의 균계(菌系) 생장(生長)에 영향(影響)을 미치는 몇가지 요인(要因)에 관한 시험(試驗))

  • Go, Seung-Joo;You, Chang-Hyun;Park, Yong-Hwan
    • The Korean Journal of Mycology
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    • v.12 no.1
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    • pp.15-19
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    • 1984
  • Some factors affecting mycelial growth of Pleurotus sajor-caju and P. ostreatus. were investigated. The optimal temperature and pH of the medium for mycelial growth of both the species were $25^{\circ}C$ to $30^{\circ}C$, and 5 to 6, respectively. The effect of carbon and nitrogen sources on mycelial growth of the species was studied using modified Waksman's special medium as the basal medium. Generally, the disaccharides and polysaccharides showed good effect for mycelial growth of P. sajor-caju, and the polysaccharides were superior to the other classes of carbon sources for mycelial growth of P. ostreatus. Generally the organic nitrogen compounds showed good mycelial growth of both the species. The mycelial growth of the species were favored by the ammonium nitrogen more than by the nitrate nitrogen source. Asparagine was superior to the other kinds of amino acid. Six percentage of glucose and 0.6% ammonium tartarate was optimum for mycelial growth of P. sajor-caju whereas the optimum concentration for the P. ostreatut was 4% glucose and 0.4% ammonium tatarate.

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Effect of Surface Treatment of Polycarbonate Film on the Adhesion Characteristic of Deposited SiOx Barrier Layer (폴리카보네이트 필름 표면 처리가 증착 SiOx 베리어층 접착에 미치는 영향)

  • Kim, Gwan Hoon;Hwang, Hee Nam;Kim, Yang Kook;Kang, Ho-Jong
    • Polymer(Korea)
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    • v.37 no.3
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    • pp.373-378
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    • 2013
  • The interfacial adhesion strength is very important in $SiO_x$ deposited PC film for the barrier enhanced polycarbonate (PC) flexible substrate. In this study, PC films were treated by undercoating, UV/$O_3$ and low temperature plasma and then the effect of physical and chemical surface modifications on the interfacial adhesion strength between PC film and $SiO_x$ barrier layer were studied. It was found that untreated PC film shows significantly low interfacial adhesion strength due to the smooth surface and low surface free energy of PC. Low temperature plasma treatments resulted in the increase of both surface roughness and surface free energy due to etching and the appearance of polar molecules on the PC surface. However, UV/$O_3$ treatment only shows the increase of surface free energy by developed polar molecules on the surface. These surface modifications caused the enhancement of surface interfacial strength between PC film and $SiO_x$ barrier. In the case of undercoating, it was found that the increase of surface interfacial strength was achieved by adhesion between various acrylic acid on acrylate coated surface and $SiO_x$ without increase of polar surface energy. In addition, the barrier property is also improved by organic-inorganic hybrid multilayer structure.

Changes in the physicochemical quality, functional properties, and actinidin content of kiwifruit (Actinidia chinensis) during postharvest storage (후숙시기 동안 참다래의 품질, 기능성 및 액티니딘 함량 변화 조사)

  • Nam, Seung-Hee
    • Food Science and Preservation
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    • v.23 no.3
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    • pp.291-300
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    • 2016
  • Kiwifruit (Actinidia chinensis, Hayward) was stored at $25^{\circ}C$ for 0~30 days and investigated to find out the optimum storage time to obtain the best physical and functional properties for consumers' preference. Kiwifruits was stored at different time period (0, 5, 10, 15, 20, and 30 days) for investigating their physiochemical quality, nutritional components, and functional characteristics. Kiwifruits stored for 20~30 days showed the best physiochemical quality such as higher total acidity and proper firmness. They were also more enriched with dietary fibers, free sugar, and organic acid, although no significant changes were observed in crude protein, crude fat, and moisture content. For functional properties, kiwifruits stored for 20 days showed significantly higher contents of total phenolics, flavonoids, and actinidin. In addition, it showed stronger antioxidant activity, whitening effect, and proteolytic activity when compared with other samples. SDS-PAGE analysis showed the presence of actinidin enzyme in kiwifruits. These results indicated that the kiwifruits stored for 15~20 days possessed excellent quality and high concentrations of nutritional and functional compounds, which could be best for both fresh consumption and product processing.

Variation of Bolting at Cultivation of Different Regions and Molecular Characterization of FLC homologs in Angelica gigas Nakai (재배 지대에 따른 참당귀의 추대 변이와 FLC 유전자 특성)

  • Kim, Young-Guk;Yeo, Jun-Hwan;An, Tae-Jin;Han, Sin-Hee;Ahn, Young-Sup;Park, Chung-Beom;Jang, Yun-Hee;Kim, Jeong-Kook
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.5
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    • pp.359-364
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    • 2012
  • This study were carried out to find bolting response of cultivation in different regions and to isolate FLC (FLOWERING LOCUS C) homologs in Angelica gigas Nakai. The mean temperature of different regions, ordering in altitude, were as follows: 100 m > 350 m > 530 m > 700 m. The largest amount of rainfall was occurred in the region of 350 m while the longest time of sunshine was occurred in the region of 100 m. The content of soil chemical properties in regions showed pH 6.2 ~ 7.4, T-N 0.17 ~ 26, organic mater $1{\sim}32gkg^{-1}$, $P_2O_5$ ${151{\sim}664_{mgkg}}^{-1}$, exchangeable potassium and calcium and magnesium were 0.78 ~ 1.15, 3.9 ~ 10.0, ${0.7{\sim}3.2_{cmol}}^{+kg-1}$. L5 line of A. gigas was occurred in bolting at all regions, but the bolting ratio was 60.0% in 700 m region with non-mulching treatment. Manchu of A. gigas was not occurred in bolting at all regions. The accumulation bolting ratio of L5 line by non-mulching was higher than that of mulching as 90.4% and 72.8% in 100 m region. The MADS-box transcription factor FLC is one of the well-known examples as a strong floral repressor. We decided to isolate FLC homologs from A. gigas as a starting point of flowering mechanism research of this plant. We have isolated two RT-PCR products which showed very high amino acid sequence homology to Arabidopsis FLC.

Sensory Evaluation and Changes in Physiochemical Properties, and Microflora and Enzyme Activities of Pumpkin-added Kochujang (호박을 첨가하여 제조한 고추장의 숙성 중 성분 변화 및 관능적 특성)

  • Choo, Jong-Jae;Shin, Hyun-Ju
    • Korean Journal of Food Science and Technology
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    • v.32 no.4
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    • pp.851-859
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    • 2000
  • In the present study, changes in physicochemical and sensory properties, and microflora and enzyme activities, by addition of pumpkin (1, 2 and 5% pumpkin on the weight basis), of Sunchang sikhe kochujang, the most famous traditional kochujang, were investigated. Moisture content appeared to be reduced during fermentation, possibly due to exposure to the sun. Patterns of pH changes were rather complicated. pH of control kochujang was gradually reduced from the begining of fermentation up to 60 days of fermentation. Thereafter, it tended to be increased but again reduced after 90 days of fermentation. On the other hand, titratable acidity increased with fermentation reaching at the highest level at 90 day of fermentation and then slowly decreased. The addition of pumpkin induced a reduction in pH and an increase in titratable acidity, but this was due to organic acid contained in pumpkin itself. Contents of reducing sugar and amino nitrogen were increased by the addition of pumpkin in relation to the level of addition. Ethanol content was highly increased by the addition of pumpkin. Bacterial and yeast count, and activities of amylase and protease were not affected by the addition of pumpkin. Sensory evaluation test revealed that the addition of pumpkin improved sweet and savory taste, but color turned to be undesirable. However, none of tested parameters except color showed statistical significance.

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Effect of Fermentation Temperature on Quality Characteristics of Apple Wine (발효온도가 사과와인 품질 특성에 미치는 영향)

  • Kwak, Han Sub;Seo, Jae Soon;Bae, Haejung;Lee, Hwajong;Lee, Youngseung;Jeong, Yoonhwa;Kim, Misook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.1
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    • pp.155-159
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    • 2016
  • The objective of this study was to investigate the effect of fermentation temperature on quality characteristics of apple wine. Apple wine mashes were fermented in 15, 20, and $25^{\circ}C$ water bathes for 9 days. The pH levels of all samples were below 4 from 24 h of fermentation until the end. Total acidities of 0.05% acetic acid solution were 7.8, 7.4, and 7.0% in the 15, 20, and $25^{\circ}C$ fermented samples, respectively. The evaporation of esters generated by combining alcohol and organic acids might be the reason for lower total acidity for high temperature fermentation. Alcohol contents of the 20 and $25^{\circ}C$ fermented samples were 6.5 and 6.6% (v/v), respectively, whereas that of the $15^{\circ}C$ fermented sample was 5.6% (v/v) and significantly lower than the others (P<0.05). Methanol contents were 0.68, 0.82, and 1.69 mg/L in the 15, 20, and $25^{\circ}C$ fermented samples, respectively. Fermentation at higher temperatures generated higher methanol content in apple wine. On the other hand, acetaldehyde contents were 3.43, 2.39, and 1.02 mg/L in the 15, 20, and $25^{\circ}C$ fermented samples, respectively, due to the lower boiling point of acetaldehyde ($20.2^{\circ}C$). Based on the results, a fermentation temperature of $20{\sim}25^{\circ}C$ is effective for apple wine fermentation.

Mix Design Conditions at Early Curing Age of PCS-Coating Material Effected on Improvement in Bond Strength of Coated Rebar (도장철근의 부착강도 개선에 영향을 미치는 초기재령에서의 PCS 도장재 배합조건)

  • Jo, Young-Kug;Park, Dong-Yeol;Kim, Wan-Ki
    • Journal of the Korea Concrete Institute
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    • v.25 no.1
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    • pp.73-80
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    • 2013
  • Polymer cement slurry (PCS) made from organic polymer dispersion and cement has good adhesion to steel, waterproofness and acid resistance due to being of polymer films formed in cement slurry. The purpose of this study is to evaluate the mix design conditions at early curing age of PCS-coating material effected on improvement in bond strength of coated rebar. The test pieces are prepared with two types of polymer dispersions such as St/BA and EVA, four polymer-cement ratios, two types of cement, four coating thicknesses and three curing ages, and tested for the bond strength test. From the test results, in general, bond strength of PCS-coated rebar is better than that of uncoated rebar and epoxy-coated rebar. It is also high bond strength at curing ages of 7-day or less, and coating thicknesses of $75{\mu}m$ and $100{\mu}m$. The maximum bond strength of PCS-coated rebar at curing age of 3-hour is almost same as that of curing age of 1-day and 7-day. The maximum bond strength of PCS-coated rebar with ultra high-early strength cement and St/BA at polymer-cement ratio of 80%, and coating thickness of $100{\mu}m$ is about 1.52 and 1.58 times respectively, the strength of uncoated rebar and epoxy-coated rebar. It is apparent that the curing age, coating thickness, type of polymer and cement are very important factors to improve the bond strength of PCS-coated rebar to cement concrete. We can have basic information that PCS-coated rebar with curing age at 3-hour and coating thickness of $100{\mu}m$ can replace epoxy-coated rebar.

황토의 인흘착 성능평가

  • 허영오;손지호
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 1997.10a
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    • pp.65-66
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    • 1997
  • 우리나라의 대부분 하천과 인공댐의 경우 조류발생에 기여하는 영양염은 질소, 인 그리고 규소 등인데 특히 인이 생산제한 인자로 작용하고 있다. 따라서 인을 적절히 제거할 경우에는 조류 발생에 의한 수질의 악화와 수이용의 저해요인을 배제할 수 있어 이에 대한 연구가 많이 진행되어 왔다. 지금까지와 연구는 인의 화학적 응집 침전법, 생물학적 처리법에 대하여 수행되어 왔으나 설비자금, 운영비, 운전기술, 슬러지 생성 그리고 제거효율 등에서 만족스런 결과가 도출되지 못하여 현장 적용을 하지 못하고 있는 실정이다. 이러한 현실을 감안하여 우리의 자연 환경에서 쉽게 자할 수 있는 황토를 모재로하여 Al3-과 Fe3-, Ca2-을 적절히 배합하여, 주로 인을 선택적으로 제거하 고자 개발된 황토의 흡착능력 그리고 흡착 메카니즘에 대한 기본적인 연구를 하고자 (1) 등온흡착실험을 통하여 흡착용량을 평가하고 (2) 흡착제거속도를 평 가하였고 (3)파과시간 및 흡착특성을 파악하기 위해 column 흡착실험을 하였다 또한 (4) 황토내의 Al3-, Fe3-과 Ca2- 등이 인의 화학적 흡착에 기여하는 정도를 파악하여 흡착메카니즘을 규명하고자 하였다. 먼저 흡착용량실험을 위하여 PO3-4-P 농도 3ppm의 용액 200mf에 황토 0.2g, 0.5g, 1.0g, 2.0g을 각각 투여한 후 충분한 흡착평형이 일어나게 24시간 동 안 130rpm으로 $25^{\circ}C$ 등온반웅조에서 저어주어 흡착평형에 도달하면 상등액을 GF/C Filter로 여과한 후, 여액에 대해 PO3-4-P의 농도를 Ascrobic he건법으로 측정한 결파, Freundlich 등온흡착식에 의하면 K값은 17.34와 16.28이었으며 1/n 값은 1.32와 1.42로 인흡착 성능이 뛰어난 것으로 평가되었다. 둘째, 흡착속도 실험은 PO3-4-P 농도 1.5ppm의 용액 2f에 259의 황토를 투여하고 충분한 혼합이 일어날 수 있도록 170rpm으로 교반하면서 시간별 용액 의 농도 변화를 측정한 결과, 0.45mg/g/m교의 속도로 15분만에 94.3%의 인 제거 효율을 보였다. 셋째, 직경 12mm의 glass column에 황토를 209 채우고 1.5ppm의 PO34P 용액을 2.Sne11in의 유량으로 통수 시킨 후, 시간에 따른 농도 변화를 측정한 결과, 원수 농도의 50%에 해당하는 파과점까지 약 70시간 만에 도달하였다. 넷째, Al3-, Fe3-과 Ca2- 등이 화학적 흡착에 기여하는 정도를 파악하기 위 하여 황토 2g에 대하여 Hieltijes and Lijklema 방법에 의해 Adsorbed-p, Nonapatite inorganic-P(NAI-P), Apatite-p, Organic-P로 구분하여 분석하고, 총인(Total Phosphorus)을 Standard Methods에 따라 Persulfate digestion후 0.45 m membrane 여지 여과하여 여액에 대해 PO3-4-P의 농도를 Ascorbic Acid 법으로 측정한 결과, NAI-P가 가장 큰 비율을 차지하였고, 부원료로 첨가된 금속 양이온 중 Fe3-이온이 흡착에 기여하는 정도가 가장 큰 것으로 평가되었다.

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Evaluate spent mushroom substrate for raising bed soil of rice (버섯 수확 후 배지의 수도용 상토로써의 활용가능성 평가)

  • Oh, Tae-Seok;Park, Youn Jin;Kim, Tae-Kwon;Kim, Chang-Ho;Cho, Yong-Koo;Kim, Seong-Min;Shin, Dong-Il;Koo, Han-Mo;Jang, Myoung-Jun
    • Journal of Mushroom
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    • v.13 no.3
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    • pp.250-255
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    • 2015
  • In this study spent mushroom substrate has ingredient raising rice bed soil. spent mushroom substrates are organic content is 60.72% were nitrogen - phosphoric acid - potassium is 1.39 - 0.89 - 0.81% of the chemical characteristics determine. Post-harvested mushroom substrates of the stabilization process, the temperature of the 20 days time progress in the pH of the rise and fall of temperature down were germination index also 77, as identified, Spent mushroom substrate bed soil for raising rice Ingredient to take advantage of the 20 days or more stabilization process needed to be investigated. Rice seed germination characteristic is in the common bed soil for raising rice ingredients manufactured control group and the comparison in spent mushroom substrate is 10% or less of a mixed experimental population of the germination rate is 82% was more than average days to germination and germination energy, even a statistical significant difference is or control group than good level was ok. Growth initial also spent mushroom substrate is 10% or less of a mixed experimental population of shoot dry matter (top) and grave less than control group higher as confirmed spent mushroom substrates are bed soil for raising rice ingredients are likely to take advantage of the high, as was the judge.

Influence of Cilnidipine on Catecholamine Release Evoked by Cholinergic Stimulation and Membrane Depolarization in the Perfused Rat Adrenal Gland

  • Lim, Dong-Yoon;Woo, Seong-Chang;Ko, Suk-Tai
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2001.11a
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    • pp.95-95
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    • 2001
  • Ciinidipine (FRC-8635) is a newly synthesized novel DHP type of organic Ca$\_$2+/channel blockers that have been developed so far in Japan (Yoshimoto et al., 1991 : Hosono et at., 1992). It also has a blocking action on L-type voltage-dependent Ca$\^$2+/channel (VDCCs) in the rabbit basilar artery (Oike et al., 1990) and a slow-onset and long-lasting hypotensive action in clinical and experimental studies (Ikeda et al., 1992 ; Tominaga et al., 1997). Recent electrophysiological data indicate that cilnidipine might be a dual-channel antagonist for peripheral neuronal N-type and vascular L-type Ca$\^$2+/channels (Oike et al., 1990 ; Fujii et al., 1997; Uneyama et at., 1997). However, little is known about the involvement of N-type VDCCs in contributing to the muscarinic receptor-mediated CA secretion. Therefore, the present study was attempted to investigate the effect of cilinidipine on secretion of catecholamines (CA) evoked by ACh, high K$\^$+/, DMPP and McN-A-343 from the isolated perfused rat adrenal gland. Cilnidipine (1-10 ${\mu}$M) perfused into an adrenal vein for 60 min produced dose- and time-dependent inhibition in CA secretory responses evoked by ACh (5.32${\times}$10$\^$-3/M), DMPP (10$\^$-4/ M for 2 min) and McN-A-343 (10$\^$-4/ M for 2 min). However, lower dose of lobeline did not affect CA secretion by high K$\^$+/(5.6${\times}$10$\^$-2/ M), higher dose of it reduced greatly CA secretion of high K$\^$+/. Cilnidipine itself did also fail to affect basal catecholamine output. Furthermore, in adrenal glands loaded with cilnidipine (10 ${\mu}$M), CA secretory response evoked by Bay-K-8644 (10 ${\mu}$M), an activator of L-type Ca$\^$2+/channels was markedly inhibited while CA secretion by cyclopiazonic acid (10 ${\mu}$M), an inhibitor of cytoplasmic Ca$\^$2+/-ATPase was no affected. Moreover, $\omega$-conotoxin GVIA (1 ${\mu}$M), given into the adrenal gland for 60 min, also inhibited time-dependently CA secretory responses evoked by ACh and high K$\^$+/.

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