• Preventive Nutrition and Food Science
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• v.14 no.4
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• pp.354-357
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• 2009

Three compounds, vanillic acid, p-hydroxylcinnamic acid, p-hydroxybenzaldehyde have been isolated from the ethylacetate extract of Sparganium stoloniferum Buch.-Ham roots using silica gel open column chromatography, preparative thin-layer chromatography (pTLC) and reverse phase high performance liquid chromatography. The structures of the compounds were established on the basis of IR, extensive 1D NMR, and MS analyses. The ethylacetate (EtOAc) extract, vanillic acid, and p-hydroxybenzaldehyde showed $\alpha$-glucosidase inhibition activity of 72.71%, 20.13%, and 30.42%, at the concentration of 10 ${\mu}g/mL$, respectively. The EtOAc extract exhibited strong antioxidant activity with an $IC_50$ value of 24.37 ${\mu}g/mL$ against DPPH radical scavenging activity, the vanillic acid, p-hydroxylcinnamic acid, and p-hydroxybenzaldehyde with an $IC_50$ value of 2.10 ${\mu}M$, 1.59 ${\mu}M$, and 2.72 ${\mu}M$ against DPPH, respectively.

• Analytical Science and Technology
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• v.13 no.1
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• pp.22-26
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• 2000

2-Hydroxybenzaldehyde-5-Nitro-pyridylhydrazone (2HB-5NPH) was synthesized and its application in the spectrophotometric determination of vanadium ion(IV) was studied in the presence of surfactant. The optimum conditions of pH, solvent effect, concentration of ligand and surfactant were evaluated. The procedure was applied to determination of vanadium (IV) in mixture sample and real sample with satisfactory results (recovery ${\geq}$ 97% ; relative standard deviation ${\leq}$ 3.0% in the concentration range of $0{\sim}1.5{\mu}g/mL$ ; detection limit, $0.02{\mu}g/mL$ in solution).

• YAKHAK HOEJI
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• v.16 no.4
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• pp.162-175
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• 1972

Fifty six compounds of 4-methylthiosemicarbazone and thiorcarbohydrazone derivatives were prepared and subjected to biological tests. The following five compounds, 2-hydroxybenzaldehyde monothiocarbohydrazone (2),4-methylbenzaldehyde monothiocarbohydrazone (8), 1-(2-hydroxybenzaldehyde)-5(4-hydroxy-3-methoxybenzaldehyde) dithiocarbohydrazone (45), 1-(2-hydroxybenzaldehyde)-5-furfural dithiocarbohydrazone (46) and 1-benzaldehyde-5-cinnamaldehyde dithiocarbohydrazone (49) exhibited marked antimicrobial activity against E. coli, St. aureus and P. chrysogenum. In addition to these compounds, 3-methoxybenzaldehyde monothiocarbohydrazone (12) and 4-methylbenzaldehyde dithiocarbohydrazone (29) showed marked inhibition of HeLa cell growth at the concentration of 10 ${\nu}$g/ml. It was generally observed that most compounds demonstrated significant antifungal activity against P. chrysongenum but only one compound, 3-hydroxy-4-methoxybenzaldehyde dithiocarbohydrazone (39), exerted antituberculosis activity against M. tuberculosis H$_{37}$ RV at the concentration of 10 ${\nu}$g/ml.

• Journal of the Korean Wood Science and Technology
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• v.39 no.1
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• pp.104-112
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• 2011

There have been few reports on the constituents and biological activity of stem bark of $Acer$ $tegmentosum$, and no phytochemical and biological studies have been reported for stem wood of $A.$ $tegmentosum$. Two flavan 3-ols (1 and 2), three phenolic acid/alcohols (3~5), and two coumarins (6 and 7) were isolated from the stem wood of $A.$ $tegmentosum$ by repeated column chromatography. The structure of isolated compounds were identified as (+)-catechin (1), (-)-epicatechin (2), $p$-hydroxybenzaldehyde (3), syringic alcohol (4), $p$-tyrosol (5), scopoletin (6), and cleomiscosin A (7) on the basis of spectroscopic evidences such as $^1H$-NMR, $^{13}C$-NMR, 2D-NMR and MS spectrum. $p$-Hydroxybenzaldehyde (3), syringic alcohol (4), scopoletin (6), and cleomiscosin A (7) have not been reported from this plant so far. (+)-Catechin (1) and (-)-epicatechin (2) showed the higher 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than butylated hydroxyanisole (BHA) used as a positive control.

• Journal of the Korean Applied Science and Technology
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• v.20 no.3
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• pp.198-203
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• 2003

In this study, we synthesized p-hexyloxybenzaldehyde(HBA) by using p-hydroxybenzaldehyde and n-hexylchloride. p-dodecylaniline(DDA) prepared by nitration and reduction of dodecylbenzene. The schiff base, p-dodecylbenzylidene-$p^\prime$-hexyloxyaniline (DBHA) was synthesized by reaction of HBA and DDA. The color of synthetic compound was pale-brown and the yield was 62%. All the synthetic compounds were identified by TLC, FT-IR and ${^{i}H}$-NMR.In this study, we synthesized p-hexyloxybenzaldehyde(HBA) by using p-hydroxybenzaldehyde and n-hexylchloride. p-dodecylaniline(DDA) prepared by nitration and reduction of dodecylbenzene. The schiff base, p-dodecylbenzylidene-p'-hexyloxyaniline(DBHA) was synthesized by reaction of HBA and DDA. The color of synthetic compound was pale-brown and the yield was 62%. All the synthetic compounds were identified by TLC, FT-IR and $^1H$-NMR.

• Journal of the Korean Chemical Society
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• v.43 no.5
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• pp.522-526
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• 1999

2-Hydroxybenzaldehyde-5-nitro-pyridylhydrazone (2HB-5NPH) was synthesized and its application to the spectrophotometric determination of iron was studied. The reagent reacts with iron in the pH range 6.0-7.5 to form a yellow coIored 1:2 chelate which is very stable in methanol solution. Beer's law is obeyed in the concentration range 0.05∼2.0 ${\mu}gmL^{-1}$ iron and separation procedure using a short column filled with Amberlite XAD-7 nonionic chelating resin is proposed for the spectrophotometric determination of traces of iron. The influence of several ions as interference was discussed. The separation of Fe(III) ion from the mix-ture solution were carried out with the buffer solution (pH 5.0) and 0.25M HCl as eluents.

• Journal of Pharmaceutical Investigation
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• v.29 no.1
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• pp.47-53
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• 1999

Gastrodia elata (GE) is an oriental medicinal herb which has been used traditionally for the treatment of various brain diseases including convulsion and epilepsy. The purpose of this study was to determine pharmacokinetic parameters of 4-hydroxy-3-methoxybenzaldehyde (HMBA) and p-hydroxybenzaldehyde (PHBA), constituents of GE, in rats. Male rats were cannulated in the femoral vein, femoral artery, bile duct and ureter. They received a single i.v. bolus dose of either HMBA or PHBA through the femoral vein. The concentration of HMBA or PHBA in plasma, bile and urine samples were analyzed by reversed-phase HPLC. HMBA and PHBA have very short half-lives, i.e. 4.03 and 2.26 minutes respectively. Most of HMBA and PHBA were thought to be eliminated through metabolism as the metabolized fraction approaches unity. Derivatives of HMBA or PHBA with longer biological half-lives should be designed to develop better anticonvulsants and more complete qualitative and quantitative understanding of the overall pharmacokinetic fate of these compounds awaits further investigation.

• Archives of Pharmacal Research
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• v.27 no.4
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• pp.386-389
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• 2004

Repeated column chromatography of the CHCI_3-soluble fraction of Zingiber zerumbet led to the isolation and identification of two aromatic compounds, p-hydroxybenzaldehyde (1) and vanillin (2), and six kaempferol derivatives, kaempferol-3,4',7-O-trimethylether (3), kaempferol-3-O-methylether (4), kaempferol-3,4'-O-dimethylether (5), 4'-O-acetylafzel in (6), kaempferol-3-O-(4-O-acetyl-$\alpha$-L-rhamnopyranoside)], 2',4'-O-diacetylafzelin (7), kaempferol-3-O-(2,4-O-diacetyl-$\alpha$-L-rhamnopyranoside)], and 3',4'-O-diacetylafzelin (8), kaempferol-3-O-(3,4-O-diacetyl-$\alpha$-L-rhamnopyranoside)]. The structures of 1-8 were identifed by analysis of spectroscopic data as well as by comparison with published values. This is the first report on the isolation of compounds 1-3 from this plant.

• Korean Journal of Pharmacognosy
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• v.38 no.3 s.150
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• pp.291-295
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• 2007

Eight compounds were isolated from the whole plants of Euphorbia supina (Euphorbiaceae) through repeated silica gel, YMC gel and Sephadex LH-20 column chromatography. Their chemical structures were elucidated as 7-hydroxy-6-methoxycoumarin (scopoletin) (1), p-hydroxybenzaldehyde (2), methyl gallate (3), gallic acid (4), quercetin (5), quercetin $3-O-{\alpha}-L-arabinofuranoside$ (avicularin) (6), kaempferol $3-O-{\alpha}-L-arabinofuranoside$ (juglanin) (7) and kaempferol $3-O-{\beta}-D-glucopyranoside$ (astragaline) (8) by spectroscopic (NMR and MS) analysis.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.10
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• pp.1633-1637
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• 2005

Five different plant cell wall degrading enzymes were tested for their ability to release p -hydroxybenzoic acid from carrot alcohol insoluble residue (AIR) and cellulose fraction. Phenolics of AIR from cell wall materi민 (CWM) in carrot were found to consist primarily of p-hydroxybenzoic acid (1,977 $\mu$g/g AIR) with minor contribution from vanillin (55.9 $\mu$g/g AIR), ferulic acid (13.6 $\mu$g/g AIR) and p-hydroxybenzaldehyde (10.6 $\mu$g/g AIR). The contents of ferulic acid in Driselase, Cellulase, Macerozyme R-200, Macerozyme R-10 and Sumyzyme MC were 2,319, 2,060, 391, 95.2, 34.1 $\mu$g/g, respectively. Incubation of Driselase with AIR released only 2.8$\%$ of the total 4 M NaOH extractable p-hydroxybenzoic acid. These results indicate that commercial five plant cell wall dograding enzymes can not release P-hydroxybenzoic acid from carrot AIR and cellulose fraction.