• IEMEK Journal of Embedded Systems and Applications
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• v.9 no.4
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• pp.205-210
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• 2014

Cache based live migration method utilizes a cache, which is accessible to both side (remote and local), to reduce the virtual machine migration time, by transferring only irredundant data. However, address space layout randomization (ASLR) is proved to reduce the memory duplicate ratio between targeted migration memory and the migration cache. In this pager, we analyzed the behavior of ASLR to find out how it changes the physical memory contents of virtual machines. We found that among six virtual memory regions, only the modification to stack influences the page-level memory duplicate ratio. Experiments showed that: (1) the ASLR does not shift the heap region in sub-page level; (2) the stack reduces the duplicate page size among VMs which performed input replay around 40MB, when ASLR was enabled; (3) the size of memory pages, which can be reconstructed from the fresh booted up state, also reduces by about 60MB by ASLR. With those observations, when applying cache-based migration method, we can omit the stack region. While for other five regions, even a coarse page-level redundancy data detecting method can figure out most of the duplicate memory contents.

• Journal of Korea Multimedia Society
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• v.16 no.3
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• pp.342-353
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• 2013

The Internet has become a part of our lives. As the number of devices with Internet accessibility increases, users can use web services with those devices anytime, anywhere. Web contents on the web page can be delivered to user in various forms for various devices and users want to use seamlessly the contents with an appropriate device. Web browser extension is function to add features that are not supported by default browser. All browsers support extensions that provide the same services for cross-browser. In this paper, We proposed object migration architecture between heterogeneous browsers by expanding our proposed mechanism that identifies objects and the information of those objects to be migrated in the web page, extracts the object and creates object after migration. For this purpose, we analyzed the extension architecture of representative browsers and investigated necessary files to develop objects migration extension. In addition, We investigated how to send and receive message among files in each browser extension and the interaction mechanism among those files. Finally, We implemented the object migration mechanisms between heterogeneous browsers.

• ETRI Journal
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• v.36 no.6
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• pp.988-998
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• 2014

For memory-based big data storage, using hybrid memories consisting of both dynamic random-access memory (DRAM) and non-volatile random-access memories (NVRAMs) is a promising approach. DRAM supports low access time but consumes much energy, whereas NVRAMs have high access time but do not need energy to retain data. In this paper, we propose a new data migration method that can dynamically move data pages into the most appropriate memories to exploit their strengths and alleviate their weaknesses. We predict the access frequency values of the data pages and then measure comprehensively the gains and costs of each placement choice based on these predicted values. Next, we compute the potential benefits of all choices for each candidate page to make page migration decisions. Extensive experiments show that our method improves over the existing ones the access response time by as much as a factor of four, with similar rates of energy consumption.

• Journal of Embryo Transfer
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• v.14 no.1
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• pp.47-57
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• 1999

Among proteins separated from methanol extract of follicular fluid with superose column, the components inducing sperm swim-up separation through sucrose layer were analysed with superose column in Smart system and SDS-PAGE. And the results obtained were as follows; The fractions of retention volume (RV) 0.83ml and RV 1.36ml separated with superose column should stimulate sperm migration and movement. However, RV 0.83 fraction was consisted of complex materials containing RV 1.36 component. RV 1.36 fraction contained a BSA analogue of 67 kilodaltons (Kd) and showed identical peak pattern with BSA fraction V. In conclusion, the protein of 67 Kd in follicular fluid should stimulate sperm migration and movement.

• YAKHAK HOEJI
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• v.55 no.3
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• pp.203-212
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• 2011

Adipocytes have been known to secrete a number of important proteins called adipokines with roles in energy metabolism, reproduction, cardiovascular function and immunity. In this study we have attempted to identify intensively secretory proteins from 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into mature adipocytes and then the cells were left in serum-free medium. The supernatant was filtrated and dialyzed. Lyophilized secretome was fractionated by two different methods, 1-D SDS PAGE and RP-FPLC. The tryptic peptides from the gel slices and the FPLC fractions were analyzed by nanoLC/ESI-MS/MS. We identified a total of 303 identical proteins from two methods, 251 proteins from 1-D gel and 184 proteins from RP-FPLC. 86 of them were listed as a secretory protein Finally, we identified many known or unknown secreted proteins existed in the low level including adiponectin, angiotensinogen, bone morphogenetic protein-1 (BMP-1), macrophage migration inhibitory factor (MIF), insulin like growth factor-II (IGF-II), interleukin-6 (IL-6), follistatin-related protein-1, minecan, and resistin. The existence of some of secreted proteins has been confirmed in RNA level. This proteomic experiment is useful for the intensive screening of secretory proteins in many kinds of other cells.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.20
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• pp.8685-8688
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• 2014

The objective of the present study was to investigate cloning, expression, and functions of the recombinant protein, Siva1. Siva1 gene was synthesized by RT-PCR from HCT116 cells. Plasmids were cleaved with the restriction endonuclease, BamH1/Sal1 and products were connected to pQE30, which underwent cleavage by BamH1/Sal1. The recombinant plasmid, pQE30-Siva1, was identified after digestion with restriction endonucleases followed by transformation into E. coli M15. Expression of Siva1 was induced by IPTG and identified by SDS-PAGE following purification with affinity chromatography. The results showed that size of Siva1 was 12 kDa, consistent with the molecular weight of the His-Siva1 fusion protein. Functional test demonstrated that Siva1 significantly inhibited the invasion and migration of HCT116 cells. It may thus find clinical application for control of cancers.

• The KIPS Transactions:PartA
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• v.15A no.6
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• pp.335-344
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• 2008

We propose an adaptive garbage collection technique for hybrid flash memory which has both SLC and MLC. Since SLC area is fast and MLC area has low cost, the proposed scheme utilizes the SLC area as log buffer and the MLC area as data block. Considering the high write cost of MLC flash, the garbage collection for the SLC log buffer moves a page into the MLC data block only when the page is cold or the page migration invokes a small cost. The other pages are moved within the SLC log buffer. Also it adjusts the parameter values which determine the operation of garbage collection adaptively considering I/O pattern. From the experiments, we can know that the proposed scheme provides better performance compared with the previous flash management schemes for the hybrid flash and finds the parameter values of garbage collection close to the optimal values.

• BMB Reports
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• v.30 no.6
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• pp.448-452
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• 1997

Polyclonal antibody of the boar sperminogen was used to characterize the boar sperm proteinase sperminogen. Boar sperminogen was purified from the acid extracts of the washed epididymal spermatozoa by gel filtration through a Sephadex G-100 column. followed by preparative SDS-PAGE. The sperminogen band was sliced out and was eluted from the gel matrix. The purified sperminogen was used to produce the polyclonal antibody of the boar sperminogen. When characterized on a Western blot, the final preparation of sperminogen appeared as a homogenous protein with a molecular weight of 32 kDa. The relative migration of sperminogen was distinctly different from the major components of the proacrosin-acrosin system as well as all the observable proacrosin activation by-products detected on the Western blot. The sperminogen antibody, however. cross-reacted with the proacrosin-acrosin system.

• Microbiology and Biotechnology Letters
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• v.37 no.4
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• pp.405-407
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• 2009

A Bacillus subtilis mannanase gene was subcloned into an Escherichia coli- Corynebacterium lactofermentum shuttle vector pHE83, and the resultant plasmid pHE83M was transferred into an endogenous plasmid-free strain of C. lactofermentum. Mannanase produced by the recombinant C. lactofermentum (pHE83M) was secreted extracellulary at the level of 86%, and the remaining activity of mannanase was detected in the cell-free extract. The maximum mannanase productivity of the recombinant strain reached 8.1 unit/mL in the culture filtrate of LB medium. According to the zymogram of mannanase on SDS-PAGE, it was found that the mannanase produced by the recombinant C. lactofermentum could be maintained stably with a migration identical to the mannanase produced by the parental strain, B. subtilis WL-3.

• Journal of Microbiology and Biotechnology
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• v.2 no.2
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• pp.122-128
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• 1992

Post-translational modifications of the nucleocapsid protein of bovine coronavirus (Quebec strain) were investigated. Coronavirions were radiolabelled in vivo with inorganic $[^{32}P]$orthophosphate and analysed by SDS-PAGE, followed by autoradiography. A single polypeptide with a migration rate of 55 KDa was identified by metabolic phosphate labelling, demonstrating that the nucleocapsid protein of bovine coronavirus was a phosphoprotein. A gene encoding the nucleocapsid protein was inserted immediately downstream from the polyhedrin promoter of Autographa californica nuclear polyhedrosis baculovirus. Spodoptera frugiperda cells infected with this recombinant baculovirus synthesized a 55 KDa polypeptide, as demonstrated by immunoprecipitation with anti-nucleocapsid monoclonal antibody. The recombinant nucleocapsid protein synthesized in Spodoptera cells could also be labelled by $[^{32}P]$orthophosphate. Phosphoamino acid analysis showed that both serine and threonine residues were phosphorylated in authentic, as well as in recombinant nucleocapsid proteins, with a relative phosphorylation ratio of 7:3. Our studies demonstrated that the nucleocapsid protein of bovine coronavirus was a serine and threonine-phosphorylated protein and that Spodoptera insect cells were able to properly phosphorylate the relevant foreign proteins.