• Title/Summary/Keyword: phbCAB operon

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Construction of the Recombinant phbCAB Operon of Alcaligenes eutvtrphus for Accumulation of Poly-$\beta$-hydroxybu tyric Acid in Escherichia coli (Alcaligenes eutrophus phbCAB Operon의 재조합과 Poly-$\beta$-hydroxybutyric Aicd의 대장균내 축적)

  • 김경태;박진서;이용현;허태린;박해철
    • Microbiology and Biotechnology Letters
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    • v.21 no.3
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    • pp.221-228
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    • 1993

  • In order to achieve poly-beta-hydroxybutyric acid (PHB) production using recombinant DNA in various host bacterial cells, the isolation of genes for PHB biosynthesis was attempted. As a result, a 5.2kb DNA fragment containing phbCAB operon of Alcaligenes eutrophus was isolated by colony hybridization using synthetic oligodeoxyribonucleotides as probes. The constructed recmbinant plasmid pSK(+)-phbCAB operon was transferred to Escherichia coli, and the obtained transformant accumulated considerable amount of PHB.

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