• Title/Summary/Keyword: phenolic contents

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Phenolic Compounds Contents of Rusty-Root Tolerance Ginseng Lines in 6-Year Old Root (6년생 적변내성 인삼계통의 Phenolic Compounds 함량 특성)

  • Lee, Sung-Sik
    • Journal of Ginseng Research
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    • v.26 no.3
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    • pp.165-169
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    • 2002
  • Experiments were carried out to select marker for rusty tolerance ginseng root using high rusty degree lines(HRL) and low rusty degree lines (LRL) in ginseng plant. A strong positive correlation was detected between degree of rusty-root in 4-year-root and that in 6-year-root. The contents of phenolic compounds among samples were not different in stele and branch & fine roots. The contents of phenolic compounds of rusty-roots was higher than that of healthy-roots in cortex, but those of high 겨sty degree lines (HRL) were not different compared with low rusty degree lines (LRL) in cortex using same rusty-degree samples. These suggest that phenolic compounds in cortex tissue were not adequate as a marker to select rusty tolerance ginseng roots. The contents of phenolic compounds of rusty-roots were higher than that of healthy-roots in epidermis, and those of HRL were higher than LRL in epidermis using same rusty-degree samples. These suggested that the contents of phenolic compounds in epidermis tissue might be a potent marker to select rusty tolerance ginseng roots.

Changes in Total Polyphenol Contents and DPPH Radical Scavenging Activity of Agrimonia pilosa According to Harvest Time and Various Part (수확시기별, 부위별 선학초의 폴리페놀함량 및 DPPH 라디컬 소거능의 변화)

  • Jang, Sang-Hun;Yu, Eun-Ae;Han, Ki-Soo;Shin, Sung-Chul;Kim, Hee-Kyu;Lee, Sang-Gyeong
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.6
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    • pp.397-401
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    • 2008
  • Changes in the contents of total phenolic compounds in as Agrimonia pilosa well as their antioxidant capacity according to the havest time and positions were examined. The contents of the total phenolic compounds were determined by extraction with MeOH. Among havest times harvestry in July showed highest contents of the total phenolic compounds and harvestry in May showed lowest contents of the phenolic compounds. Among the 4 positions (root, branch, leaf, flower) of Agrimonia pilosa the root contained highest contents of the phenolic compounds. The antioxidant capacities of Agrimonia pilosa were increased roughly with increasing level of contents of phenolic compounds according to positions.

The determination of total phenolic and flavonoid contents in Lentinus edodes and their Glutathione S-Transferases effects

  • Hwang, Hyun-Ik;Lee, In-Son;Moon, Hae-Yeon
    • 한국생물공학회:학술대회논문집
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    • 2003.10a
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    • pp.667-671
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    • 2003
  • The total phenolic and flavonoid contents of Lentinus edodes extracts were determinated by spectrophotometrical .method, and antihepatotoxic activity was detected on glutathione S-Transferases(GST). The total phenolic contents was highest water extract than solvent(ethanol, methanol) extracts, but flavonoid content was appear on opposite. GST activity was the highest appears in water extract. This fact verified of anticancer effect indirectly of Lentinus edodes.

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Various Properties and Phenolic Acid Contents of Rices and Rice Brans with Different Milling Fractions (품종 및 도정도별 백미와 미강의 특성 및 페놀산 함량)

  • Kim, Sung-Ran;Ahn, Ji-Yun;Lee, Hyun-Yu;Ha, Tae-Youl
    • Korean Journal of Food Science and Technology
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    • v.36 no.6
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    • pp.930-936
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    • 2004
  • Effects of rice cultivars and degree of milling (DM) on composition, pasting properties, total phenolic contents, and distribution of phenolic acids were investigated. Rice and bran fractions with 94.4, 92.0, and 90.4% milling yields from brown rice of four cultivars (Odae, Nampyung, Chucheong, and Ilmi) were used. Fat and ash contents of milled rices decreased with increasing DM, whereas protein contents were not affected. In rice bran, differences in fat and ash contents by cultivars were higher than those caused by DM. With increasing DM, gelatinization temperature of rice flour decreased, whereas peak viscosity and hold viscosity at $95^{\circ}C$ increased. While cold viscosity, final viscosity, and setback varied among cultivars, DM had little effect. Total polyphenolic contents in brown rice, milled rice, and rice bran were 93.9-88.8, 30.3-71.9, and 310.0-541.6 mg catechin eq/100g, respectively. Major phenolic compounds were identified as ferulic and p-coumaric acids. Total phenolic content of brown rice (65.9-27.9 mg%) decreased with increasing DM, whereas ratio of ferulic acid composition increased. Chucheong and Ilmi varieties showed biggest reduction of phenolic acid contents by milling. In rice bran, ferulic and p-coumaric acids were 157.8-240.2 and 31.8-90.4 mg%, respectively. Contents of sinapinic, benzoic, and m-hydroxybenzoic acids in rice bran were higher than those of brown and milled rices.

Chemical compositions of the sap of Acer mono MAX in Cheonnam region. (전남지역 고로쇠나무 수액의 성분분석)

  • 현규환;정현채;김준선
    • Korean Journal of Plant Resources
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    • v.12 no.3
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    • pp.215-220
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    • 1999
  • To find out the temporal and spatial variations in contents of sugar, phenolic compounds and mineral, the sap of Acer mono MAX. were harvested from several regions and periods, and those contents were determined by solvent fractionation, TLC, HPLC and atomic absorption spectrophotometer. Most of all the free sugar contained in the sap of Acer mono MAX. was sucrose, with range of 1.35 to 3.41%. The content of total phenolic compound in the sap of Acer mono MAX. was distributed from 0.034 to 0.151 mg/100m1, and most of phenolic acid was phloroglucinol. The contents of Na, K, Ca and Mg were ranged from 24.11 to 33.15, from 13.75 to 22.10, from 47.19 to 88.00 and from 2.48 to 6.31 ppm, respectively. Contents of sugar, phenolic compounds and mineral decreased sharply at the later harvest. Comparing two different methods of the sap harvest, bore method and groove method, the contents of compounds in the study were determined higher with groove method.

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Secondary Metabolites and Morphological Diversity in the Leaves of Perilla Landrace from Korea

  • Assefa, Awraris Derbie;Sung, Jung-Sook;Jeong, Yi-Jin;Lee, Ho-Sun;Rhee, Ju-Hee;Hur, On-Sook;Noh, Jae-Jong;Ro, Na-Young;Hwang, Ae-Jin;Lee, Jae-Eun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.64-64
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    • 2019
  • Screening and identification of genetic resources based on their phytoconstituents and morphological characters potentially provide baseline data for researchers, breeders, and nutraceutical companies who wish to formulate a nutrient-dense diet and health beneficial supplement. Thus, we evaluated the amount of total phenolic content and major phenolic compounds; examined if phenolic compounds could be used as distinguishing factors for perilla genetic resources; and investigated the relation between some quantitative and qualitative morphological characters with the contents of phenolic compounds in 360 accessions obtained from National Agrobiodiversity Center gene bank, Jeonju, Korea. Total phenolic content (TPC) was estimated using Folin-Ciocalteu colorimetric assay. Individual phenolic compounds were determined using an Ultra-High Performance Liquid Chromatography system equipped with Photodiode Array detector. Considerable variations were observed in TPC (7.99 to 117.47 mg GAE/g DE), rosmarinic acid (RA) (ND to 19.19 mg/g DE), caffeic acid (CA) (ND to 0.72 mg/g DE), apigenin-7-O-diglucuronide (ADG) (ND to 1.24 mg luteolin equivalent (LUE)/g DE), scutellarein-7-O-glucuronide (SG) (ND to 4.32 mg LUE/g DE), and apigenin-7-O-glucuronide (AG) (ND to 1.60 mg LUE/g DE). RA was the most dominant phenolic compound in most accessions (95.3%) followed by SG. The adaxial leaf color was light green, green and dark green in 13.8%, 65.0%, and 21.1 % of the accessions, respectively. 78.8% of the accessions had light green color at the abaxial side with the remaining being described as green. Most of the accessions (96.9%) were cordate shape, the remaining being eclipse. Intensities of green pigment at abaxial and adaxial leaf surfaces were correlated with contents of individual phenolic compounds and TPC whereas leaf length and width had no correlation with TPC, CA and RA, and negatively correlated with ADG, AG, and SG. Leaf shape was not related with content of phenolic compounds, color of leaves, or the length or width of leaves. Accessions IT57426, IT157434, IT267710, and IT267712 which contained relatively high contents of TPC and major phenolic compounds (RA and SG) could be used for further research in breeding and bioassay test. Our study result showed the contents of total phenolics and individual phenolic compounds along with the morphological characters could be useful distinguishing factors for perilla genetic resources.

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Identification and Quantification of Phenolic Acid Derivatives from Three Korean White and Brown Rice Samples Based on High-Resolution Mass Spectrometry (쌀(백미, 현미) 시료로부터 고해상도 질량 분석 기반의 페놀산 유도체 동정 및 정량 평가)

  • Hyemin Na;Ryeong Ha Kwon;Ju Hyung Kim;So Ah Kim;Soo-Muk Cho;Heon-Woong Kim
    • The Korean Journal of Food And Nutrition
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    • v.36 no.6
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    • pp.506-514
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    • 2023
  • Reported positive ion fragmentation of phenolic acid derivatives in rice (Oryza sativa L.) were summarized based on the literature. A total of eight phenolic acids (4 derivatives of ferulic acid, 3 derivatives of sinapic acid and p-coumaric acid) were isolated and identified from rice (raw and steamed) using UPLC-DAD-QToF/MS. Results revealed that 6-O-feruloylsurose was the major component with 3'-O-sinapoylsucorse being tentatively identified in Oryza sativa L. for the first time as a new hydroxycinnamoyl derivative in rice grains. In our study, raw brown rice had the highest phenolic acid contents with Samkwang showing higher phenolic acid content than Saeilmi and Sindongjin (12.41 vs. 7.89 and 3.10 mg/100 g dry weight, respectively). Of all varieties, brown rice had higher phenolic acid contents than white rice. These contents decreased considerably when rice was steamed whereas, p-coumaric acid and ferulic acid contents were increased. Additionally, contents of rice (raw and steamed) can be used as a fundamental report for new rice varieties.

Enhances Antioxidant Effect of Purple Sweet Potato by Roasting (볶음처리에 의한 자색고구마의 항산화 증진 효과)

  • Cho, Kye Man;Joo, Ok Soo
    • Food Science and Preservation
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    • v.19 no.5
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    • pp.735-743
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    • 2012
  • Purple sweet potato (PSP, Ipomoea batatas) has various biological effects including antidiabetic, anti-inflammatory, and antioxidant activities. In this study, the antioxidant activity of PSP after roasting were compared using DPPH, ABTS, and FRAP assays. In addition, the total phenolics and flavonoid contents, Maillard reaction products, and phenolic acid contents were measured to identify the factors that changed PSP,s antioxidant activity due to roasting. The roasted PSP exhibited significantly higher antioxidant activity than unroasted PSP. In particular, the PSP roasted at $200^{\circ}C$ for 10 min showed the highest antioxidant activity among all the PSPs that were roasted under different conditions. The total phenolic and flavonoid contents, Maillard reaction products and phenolic acid contents markedly increased, corresponding to the general increase in antioxidant activities after roasting. These results suggest that roasted PSP extracts are potential source of natural antioxidants that may be used in certain food applications.

Changes of phenolic acid contents and radical scavenging activities of ginseng according to steaming times (수삼의 증숙 횟수에 따른 페놀산 함량 변화와 라디칼 소거활성)

  • Kim, Young-Chan;Hong, Hee-Do;Rho, Jeong-Hae;Cho, Chang-Won;Rhee, Young-Kyung;Yim, Joo-Hyuk
    • Journal of Ginseng Research
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    • v.31 no.4
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    • pp.230-236
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    • 2007
  • This study was conducted to investigate the contents of the total phenolic compounds, and DPPH, ABTS radical scavenging activities of phenolic acid fractions of ginseng according to steaming times. Also the individual phenolic acid compositions and contents were analyzed by GC. The contents of the total phenolic compounds proportionally increased from 0.530 to 2.893% according to steaming times. Phenolic acid fractions were separated according to bound types, and the insoluble bound form fraction showed the highest contents followed by ester form fraction and free form fraction. The total contents of these three fractions (1.031-1.416%) were not significantly influenced by steaming times. Salicylic, cinamic, p-hydroxybenzoic, gentisic, vanillic, syringic, caffeic, ferolic acid were found in each fraction, and gentisic and ferolic acid were the major phenolic acid. Each phenolic acid fraction showed over 50% of DPPH and ABTS radical scavenging activities. There were no differences between the phenolic acid fractions according to binding types. Free radical scavenging activities were affected by a number of steaming times and augmented as steaming times increased.

Characteristics and Antioxidant Effect of Garlic in the Fermentation of Cheonggukjang by Bacillus amyloliquefaciens MJ1-4

  • Kim, Jeong Hwan;Hwang, Chung Eun;Lee, Chang Kwon;Lee, Jin Hwan;Kim, Gyoung Min;Jeong, Seong Hoon;Shin, Jeong Hee;Kim, Jong Sang;Cho, Kye Man
    • Journal of Microbiology and Biotechnology
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    • v.24 no.7
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    • pp.959-968
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    • 2014
  • The changes in the ${\beta}$-glucosidase activity, total phenolic contents, isoflavone contents, and antioxidant activities during the fermentation of cheonggukjang by Bacillus amyloliquefaciens MJ1-4 with and without garlic were investigated. The levels of total phenolic and isoflavone-malonylglycoside, -acetylglycoside, and -aglycone contents increased, whereas the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities and ferric reducing/antioxidant power (FRAP) assay results increased, but isoflavone-glycoside levels decreased during cheonggukjang fermentation. The levels of total phenolic and total isoflavone contents and the antioxidant activities were higher in cheonggukjang fermented without garlic (CFWOG) than in cheonggukjang fermented with garlic (CFWG) after 24 h of fermentation, but they were lower in CFWOG than in CFWG after 72 h of fermentation. In particular, the highest levels of total phenolic, daidzein, glycitein, and genistein were present at concentrations of 15.18 mg/g, $264.4{\mu}g/g$, $16.4{\mu}g/g$, and $31.1{\mu}g/g$ after 72 h of fermentation in CFWG, showing 82.89% in DPPH radical scavenging activity, 106.32% in ABTS radical scavenging activity, and 1.47 ($OD_{593nm}$) in FRAP assay, respectively. From these results, we suggest that the high antioxidant activity of CFWG might be related to the markedly higher levels of total phenolic contents, isoflavone-malonylglycosides, -acetylglycosides, and -aglycones achieved during fermentation.