• Journal of the East Asian Society of Dietary Life
• /
• v.19 no.6
• /
• pp.975-980
• /
• 2009

In this study we evaluated polyphenol contents, phytate contents, and antioxidant activities (DPPH radical and hydroxyl radical scavenging activity) in several soybean cultivars. The polyphenol contents of the cultivars ranged from 0.0446 mg/g to 0.0652 mg/g, with the highest level found in the WS82 cultivar, whereas and the lowest in Duyukong. The phytate content of the WS2906 cultivar was the highest at 22.28 mg/g whereas Duyukong showed the lowest level. $IC_50$ values (concentration of soybean extract exerting 50% scavenging of radicals) for DPPH radical and hydroxyl radical scavenging activity showed the highest in WS82. A positive correlation between DPPH radical scavenging activity and polyphenol contents was observed in the soybean cultivars, whereas not significant relationship between antioxidant activity and phytate content was found.

• Korean Journal of Food Science and Technology
• /
• v.32 no.2
• /
• pp.231-237
• /
• 2000

A Comparative study was performed among five Korean wheat(Greu, Eunpa, Tapdong, Allgreu, Woori) and three imported wheat(Dark Northern Spring, Austrian Standard Wheat, Western White) to investigate the characteristics of minerals and phytate from Korean wheat varieties. Ash contents of kernel was higher in Korean wheat than in imported wheat and related to ash contents and darkness of flour. More contents of Fe, P, and Mg from Korean wheat grains and Fe from Korean wheat flours were observed in comparison to imported wheat varieties. Fe concentration in Korean flour show high, indicating Fe was distributed at endosperm layer of kernels and not eliminated with milling. In addition, phytate affecting availability of minerals was determined from kernels and flours. Even though Korean wheat kernels had more phytate than that of imported wheat kernels, it is believed that most of phytate was removed with milling and there was no major difference between Korean and imported wheat flours. We also observed that the content of phosphate closely parallels the content of phytate from both the flours and kernels.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.39 no.7
• /
• pp.986-991
• /
• 2010

This study was conducted to investigate the changes of mineral, oxalate and phytate during tofu processing with Korean recommended soybean cultivars. Fourteen cultivars were selected by distribution of calcium, oxalate and phytate contents. Tofu was manufactured and analyzed for quality characteristics, mineral, oxalate and phytate contents. The yield and moisture contents of tofu ranged from 137.77% in Anpyeongkong to 201.91% of Geomjeongkong 4, and 74.42 (Bongeuikong)~80.01% (Hojangkong), respectively. The lightness (L-value), redness (a-value) and yellowness (b-value) ranged from 53.05 (Dawonkong) to 86.16 (Jipumkong 2), -2.04~5.85, and from 3.28 in Geomjeongkong 4 to 16.17 of Cheongjakong, respectively. The hardness of tofu with selected soybean cultivar ranged from 537.36 g (Dongpuktae) to 1696.05 g (Jinpumkong 2). The highest calcium content was 1.488 mg/g in Dawonkong, oxalate and phytate in tofu were high at 0.40 mg/g in Geomjeongkong 4 and 0.41 mg/g in Dawonkong, respectively. The transfer ratios of oxalate and phytate from soybean to tofu were ranged from 0.31~19.70 and 0.41~19.70%, respectively.

• Journal of Applied Biological Chemistry
• /
• v.52 no.1
• /
• pp.33-37
• /
• 2009

A saccharified-rice was fermented using Leuconostoc(Ln.) mesenteroides KC51 strain in various dry matter (DM) contents (4%, 8%, and 12%) at $30^{\circ}C$ for 18 h. The changes of viable cell number, acid production and phytate degradation in saccharified-rice during fermentation were investigated. The viable cell population of Ln. mesenteroides KC51 was increased rapidly in proportion to DM contents during the 9 h of cultivation. The changes of pH and titratable acidity in saccharified-rice were dependent on DM contents. At high DM content (12%), the viable cell number of Ln. mesenteroides KC51 increased to 9.56 log CFU/g after 6 h of fermentation. The pH and titratable acidity reached to pH 3.38 and 0.93% after 18 h of fermentation, respectively. The phytate, known as an antinutrient factor, in saccharified-rice was degraded by Ln. mesenteroides KC51 cultivation. The decrease of phytate during fermentation approximately coincided with the increase of Ln. mesenteroides KC51 population observed in fermented saccharified-rice. Regardless of DM contents, the levels of phytate were reduced to around 50% of initial concentration.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.39 no.8
• /
• pp.1149-1155
• /
• 2010

This study was conducted to investigate the changes of quality characteristics, and mineral, oxalate and phytate content during soymilk process. The yields of soymilk ranged from 8.43 mL/g in Bokwangkong to 9.15 mL/g in Bongeuikong and Hannamkong, and total soluble solid contents were ranged from $4.37^{\circ}Brix$ in Anpyeongkong to $7.17^{\circ}Brix$ in Bongeuikong, respectively. The pH and total acidity of soymilk ranged from 6.43 to 6.86 and from 1.48% to 1.65%, respectively. The viscosity of soymilk was the highest value of 20.80 cP in Hannamkong and the lowest value of 15.73 cP in Dawonkong. The highest value of calcium content of soymilk was 1.589 mg/g in Seonheukkong, and oxalate and phytate in soymilk were high at 2.14 mg/g in Hannamkong and 2.18 mg/g in Anpyeongkong, respectively. The transfer ratio of oxalate from soybean to soymilk was the highest value of 77.6% in Jinpumkong 2, and one of the phytate was the highest value of 87.5% in Dongpuktae and the lowest value of 13.9% in Hojangkong.

• Food Science and Biotechnology
• /
• v.17 no.5
• /
• pp.1122-1127
• /
• 2008

A high dietary oxalate intake may lead to calcium oxalate (CaOx) kidney stones in the gastrointestinal tract. Most soy foods contain high concentrations of oxalate and/or phytate. This study analyzed the changes in oxalate (Ox), phytate ($InsP_6$), and calcium (Ca) during soymilk processing from the seeds of Korean recommended soybean cultivars (cvs). The contents of Ox, $InsP_6$, and Ca in 21 cvs ranged from 14, 108, and 148 to 231, 279, and 246 mg/100 g of dry seed, respectively. Seven cultivars were selected from the 21 cvs by the distributions of Ox, $InsP_6$, and Ca. Then, each contents of soymilk prepared from the 7 cvs were determined. All contents were lower in soymilk than in seeds, but the Ox to $InsP_6$ ratios changed from varying ratios (0.1-0.8) to normal ratios (0.8-1.0) in all cvs except 'Paldalkong'. Consequently, during soymilk processing, the Ox content was decreased and the $InsP_6$ content was remained higher than the Ox content although the Ox was likely to be less reductive than the $InsP_6$. These results may provide better information for minimizing the risk of formation of Ox kidney stones due to consumption of soy products.

• Applied Biological Chemistry
• /
• v.39 no.1
• /
• pp.32-38
• /
• 1996

Effects of microwave heating on the content of phytic acid and phosphorus of soaked soybean were investigated. Phytic acid content of Danwon, Marly and Amsoy cultivars were found to be 19.19 mg, 18.38 mg, and 16.73 mg/g defatted soybean respectively. Inorganic phosphorus content of soybeans was significantly increased during microwave heating, while phytic acid and phytate phosphorus was gradually decreased. Microwave heating was more effective than autoclaving in reducing the phytate contents. It was also found that microwave heating to soybean of low moisture content was more effective than that of high moisture content for decreasing the phytic acid content. Soaking in 2.5% sodium chloride, 2% sodium bicarbonate, and mixed salt solution for 12 hrs was not effective on reducing the contents of phytic acid and phytate phosphorus, but microwave-heating after soaking in above solutions greatly decreased the contents of phytic acid and phytate phosphorus, whereas significantly increased inorganic phosphorus of soybeans.

• Applied Biological Chemistry
• /
• v.32 no.4
• /
• pp.321-326
• /
• 1989

Nitrogen and phytate solubility of perilla seed flour were influenced by the following factors: pH, centrifugal force, temperature and the presence of salt. The nitrogen solubility of perilla seed flour was minimum$(17.1{\sim}18.0%)$ at the pH range of $(4.0{\sim}5.0)$ and maximum(92.3%) at pH 11.0, while phytate solubility was the highest(48.5%) at pH 4.8 and lowest(8.3%) at pH 11.0. The phytic acid content in the extract decreased with an increase in centrifugal force. However, the nitrogen content was not affected by centrifugal force. The solubility of nitrogen and phytate gradually increased as the temperature was increased from $5^{\circ}C$ to $60^{\circ}C$ The addition of calcium$(0{\sim}50mM)$ at pH 5.0 decreased the phytate solubility, but increased nitrogen solubility. The solubility of nitrogen and phytate of perilla seed protein isolate was gradually increased as pH raised further. The protein and phytate contents of the perilla seed protein isolate were 1.1 and 89.6%, respectively, compared to 5.0 and 60.1% for perilla seed flour.

• Fisheries and Aquatic Sciences
• /
• v.7 no.2
• /
• pp.51-57
• /
• 2004

Total phosphorus contents in rice bran and soybean meal were determined to be 5.81 and $2.77\%$, respectively, and $97.2\%$ of phosphorus in rice bran and $66.4\%$ in soybean meal were presented as phytate phosphorus. Optimum pH condition for hydrolysis of phytate in rice bran and soybean was determined to be in the pH range of 3.7 and 5.3. The highest activity of phytase for hydrolysis of phytate in both samples was determined to be at $55^{\circ}C$ for rice bran and $55-60^{\circ}C$ for soybean. Hydrolysis of phytate in soybean meal at pH 5.0 increased with the co-reaction or consecutive reaction with protease; however, in rice bran hydrolysis decreased with co-reaction with protease. Phytate degradation of soybean meal in the presence of pepsin at pH 2.5 showed higher than that of rice bran. Phytate degradation of rice bran in the presence of trypsin or pancreatin at pH 7.0 increased the activity around 2-times compared with the activity in the absence of trypsin or pancreatin. The results of this study suggest that hydrolysis of phytate in rice bran or soybean meal with phytase and protease may provide an alternative process for the preparation of aquacultural feed with a low level of organic phosphorus.

• Asian-Australasian Journal of Animal Sciences
• /
• v.8 no.2
• /
• pp.135-138
• /
• 1995

Soybean meal was fermented by Aspergillus usami in order to reduce phytate content. Aflatoxin B1 was not detected in the fermented soybean meal. The contents of crude protein, crude fiber, ether extract and crude ash were slightly increased following fermentation with a concomitant reduction in nitrogen free extract. Though the fermentation partly degraded proteins in the soybean meal, there was small difference in amino acid composition between the soybean meal and the fermented soybean meal. The results showed that the fermentation did not affect nutritional value of protein in soybean meal. Approximately 55% of phosphorus extracted by trichloroacetic acid was inositol hexaphosphate (phytate) in the soybean meal. The content of inositol tetra to hexaphosphates was not detected in the fermented soybean meal. These results indicated that the fermentation almost completely eliminated phytate in soybean meal. Phytase activity was not detected in the unfermented soybean meal. However, the enzyme activity in the fermented soybean meal was 167.7 U/g. When the fermented soybean meal in supplemented in formula feeds, phytase in the fermented soybean meal might partly degrade the phytate in other ingredients in the digestive tract. The fermented soybean meal is possibly used as a phytate-free protein source of feed, which contains high available phosphorus.