• Title/Summary/Keyword: pit

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Presence of Pituitary Specific Transcription Factor Pit-1 in the Rat Brain: Intracerebroventricular Administration of Antisense Pit-1 Oligodeoxynucleotide Decreases Brain Prolactin mRNA Level

  • Tae Woo Kim;Hyun-Ju Kim;Byung Ju Lee
    • Animal cells and systems
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    • v.3 no.3
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    • pp.311-317
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    • 1999
  • Prolactin (PRL) was reported to be locally synthesized in many brain areas including the hypothalamus, thalamus (TH) and hippocampus (HIP). In the pituitary lactotrophs, PRL synthesis is dependent upon a pituitary-specific transcription factor, Pit-1. In the present study, we attempted to identify Pit-1 or Pit-1-like protein in brain areas known as the synthetic sites of PRL. Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis showed the same Pit-1 transcripts in brain areas such as the medial basal hypothalamus (MBH), preoptic area (POA), TH, and HIP with the Pit-1 transcripts in the anterior pituitary (AP). Electrophoretic mobility shift assay (EMSA) was run with nuclear protein extracts from brain tissues using a double strand oligomer probe containing a putative Pit-1 binding domain. Shifted bands were found in EMSA results with nuclear proteins from MBH, POA, TH and HIP. Specific binding of the Pit-1-like protein was further confirmed by competition with an unlabeled cold probe. Antisense Pit-1 oligodeoxynucleotide (Pit-1 ODN), which was designed to bind to the Pit-1 translation initiation site and block Pit-1 biosynthesis, was used to test Pit-1 dependent brain PRL transcription. Two nmol of Pit-1 ODN was introduced into the lateral ventricle of a 60-day old male rat brain. RNA blot hybridization and in situ hybridization indicated a decrease of PRL mRNA signals by the treatment of Pit-1 ODN. Taken together, the present study suggests that Pit-1 may play an important role in the transcriptional regulation of local PRL synthesis in the brain.

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Bordered Pit Structure Observed by FE-SEM in Main Wood Species of Pinaceae Grown in Korea

  • Ahmed, Sheikh Ali;Chun, Su-Kyoung
    • Journal of the Korea Furniture Society
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    • v.17 no.3
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    • pp.23-28
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    • 2006
  • An experiment was conducted to investigate the pit structure of four kind of pine wood species grown in Korea. Torus diameter, margo width, margo lattice size, diameter of pit aperture and pit border width were taken under consideration for explaining the pit structure difference among Pinus densiflora, Pinus rigida, Pinus koraiensis and Larix kaempferi. Torus diameter was found highest in Pinus rigida and the lowest in Pinus densiflora. Margo lattice size varied with torus diameter. Wider torus lowered the margo lattice size. Highest margo width was found in Pinus rigida while the lowest one was found in Pinus koraiensis. Pit aperture diameter was found highest in Pinus densiflora and lowest in Pinus koraiensis. In Pinus rigida, pit border was found the highest and the lowest was found in Larix kaempferi. Pit aperture diameter and pit border were increased gradually from pith to bark while there was a decreasing trend in the margo lattice size measuring from the pith to bark.

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Methane Fermentation of Pit in Pond System for Ecological Treatment and Recycling of Animal Excreta (생태적 축산폐수 처리 및 재활용 연못시스템의 Pit 메탄발효)

  • Yang, Hong-Mo
    • Korean Journal of Environmental Agriculture
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    • v.18 no.2
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    • pp.191-195
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    • 1999
  • An integrated wastewater treatment pond system is developed for treatment and recycling of excreta from dairy cattle. It is composed of three ponds in series. A pit with a capacity of $10m^3$, 2-day hydraulic residence time, and overflow velocity of $1.5m^3m^{-2}day^{-1}$ is located internally in primary pond. It is designed for efficient sludge sedimentation and effective methane fermentation. It receives $5m^3/day$ of diluted cattle excreta by the water used for clearing stalls. A submerged gays collector for the recovery of methane is installed on the top of the pit. The average BOD_5 concentration of influent is 398.7mg/l. That of the effluent from primary pond is 49.2mg/l. About 88% of BOD_5 are removed in primary pond. It is assumed that about 60% of the influent BOD_5 is removed in the pit and that almost all of the carbon of the removed BOD_5 in the pit is converted to methane and carbon dioxide. Methane fermentation of the pit is well established at $16^{\circ}C$. This phenomena results from temperature stability, complete anaerobic condition, and neutral pH of the pit. Gas from the collector is almost 90% methane, less than 9% nitrogen, and less than 1% carbon dioxide. Thus a purified methane is produced, which can be used as energy source.

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Effect of Polar Components on Phase Inversion Temperatures in Systems Containing Nonionic Surfactants and Nonpolar Oils (비이온성 계면활성제, 비극성 오일을 포함한 계에서의 극성 성분의 Phase Inversion Temperature에 대한 영향)

  • Lim, Jong-Choo;Mori, Fuyuhiko
    • Applied Chemistry for Engineering
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    • v.5 no.2
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    • pp.274-284
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    • 1994
  • Phase Inversion Temperature (PIT) measurements showed that the addition of polar components such as oleyl alcohol and oleic acid to the system comprising n-dodecylpentaoxyethylene monoether ($C_{12}E_5$), nonpolar oil (n-hexadecane) and water produced large reductions in the PIT. The PIT was lowered as the additive-to-surfactant ratio in the surfactant films in the microemulsion phase was increased. Another dramatic effect of additive was the manner in which it affects the volume of the microemulsion phase at the PIT of the oil and water solubilization characteristics. Microemulsion phase volume was increased rapidly with decreasing PIT, i.e., with increasing amounts of additive in the system. Also with a decrease in PIT, the solubilization parameters of both oil and water in the microemulsion phase were strikingly increased. Even though soil removal data were not available for the conditions where our results obtained, PIT measurement seems a useful starting point for estimating conditions when middle-phase microemulsion formation and its associated high solubilization of oil can be expected.

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Experimental Study on the Adjustment Processes of Minning Pit in the Dredged Channels (준설하천의 웅덩이 적응에 관한 실험적 연구)

  • Jang, Chang-Lae;Jung, Kwan-Sue
    • Journal of Korea Water Resources Association
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    • v.43 no.7
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    • pp.657-666
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    • 2010
  • The adjustment processes of mining pits in the disturbed channels by sand or gravel mining were investigated by laboratory experiments in this study. The pit migrated with speed when the river bed was steep. The pit migrated slow and steady when the pit was filling with sand, but the pit migrated with speed after the filling processes was finished. The submerged angle of repose in the pit was nearly constant during the pit was filling. The pit was filled with sand with speed as the channel slope was increased. It took time for the pit to be filled with sand as the pit dimension was increased. This meant that the disturbed channels by sand or gravel mining to adjust the new environment was dependent on the slope of the channels and the dimension of the pits. The dimensionless pit length was short and the dimensionless pit depth was shallow as the time was increased. The dimensionless pit depth was shallow, but the dimensionless pit migration speed was increased as the dimensionless shear velocity and the migration speed of the pit were increased. The dimensionless pit depth was increased with the dimensionless bar migration speed. The shape of the pit was deformed and migrated downstream in accordance with the location and shape of the biased bar front which was developed upstream.

A STUDY OF ATTITUDES TOWARD AND USE OF PIT AND FISSURE SEALANTS BY DENTISTS IN CHON-BUK AREA (전북지역 치과개원의의 열구전색제 사용실태에 관한 연구)

  • Kim, Jae-Gon;Baik, Byeong-Ju
    • Journal of the korean academy of Pediatric Dentistry
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    • v.21 no.2
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    • pp.429-438
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    • 1994
  • Since the introduction of pit and fissure sealants in the early 1970's, many studies have been published amply documenting the efficacy of these materials as caries-preventive agents. However, the acceptance of the technique by dentist has been questionable. This survey was investigated the attitudes toward and use of pit and fissure sealants by dentist in Chonbuk area. The results were as follows: 1. Utilization of pit and fissure sealants is very high. Only 15% of dentists never use pit and fissure sealants, whereas 85% generally use pit and fissure sealants. 2. Most dentists reported that their knowledge of pit and fissure sealants was gained through dental schools education. 3. Concern about difficult to sell' to patient, poor retention of pit and fissure sealants and unsubstantiated by research were given as the main reasons why pit and fissure sealants was not used. 4. Patient demands and various educate program were given as important factors that would lead to increase use of pit and fissure sealants.

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Lunar Pit Craters Presumed to be the Entrances of Lava Caves by Analogy to the Earth Lava Tube Pits

  • Hong, Ik-Seon;Yi, Yu;Kim, Eojin
    • Journal of Astronomy and Space Sciences
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    • v.31 no.2
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    • pp.131-140
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    • 2014
  • Lava caves could be useful as outposts for the human exploration of the Moon. Lava caves or lava tubes are formed when the external surface of the lava flows cools more quickly to make a hardened crust over subsurface lava flows. The lava flow eventually ceases and drains out of the tube, leaving an empty space. The frail part of the ceiling of lava tube could collapse to expose the entrance to the lava tubes which is called a pit crater. Several pit craters with the diameter of around 100 meters have been found by analyzing the data of SELENE and LRO lunar missions. It is hard to use these pit craters for outposts since these are too large in scale. In this study, small scale pit craters which are fit for outposts have been investigated using the NAC image data of LROC. Several topographic patterns which are believed to be lunar caves have been found and the similar pit craters of the Earth were compared and analyzed to identify caves. For this analysis, the image data of satellites and aerial photographs are collected and classified to construct a database. Several pit craters analogous to lunar pit craters were derived and a morphological pit crater model was generated using the 3D printer based on this database.

Reliability and Validity of the Side-lying Instability and Prone Instability Tests in Patients with Lumbar Segmental Instability

  • Kim, Bo-Eon;Lee, Kwan-Woo;Park, Dae-Sung
    • Journal of the Korean Society of Physical Medicine
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    • v.16 no.1
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    • pp.1-7
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    • 2021
  • PURPOSE: The purpose of this study is to conduct inter-rater and intra-rater reliability tests in patients with low back pain (LBP) using the prone instability test (PIT) and side-lying instability test (SIT). We have analyzed the Korean version Oswestry disability index (K-ODI) correlations and radiograph finding (RF) for validity. METHODS: Individuals (n = 51) (mean age of 40.27 ± 13.28) with LBP for at least over a week were recruited, together with two participating physical therapist examiners. The measurement consisted of PIT, PST, K-ODI, and RF. Sensitivity (Sn), specificity (Sp), positive predictive value, negative predictive value, prevalence index, agreement %, Cohen's kappa, and prevalence-adjusted bias-adjusted kappa (PABAK) were calculated. The PIT and SIT were compared with RF for validity analysis, while PIT, SIT, K-ODI, and RF were calculated for the correlation analysis. RESULTS: The intra-rater reliability test measured for the PIT (kappa = .79, PABAK = .88) and SIT (kappa = .73, PABAK = .84), and inter-rater reliability test measured for the SIT (kappa = .80, PABAK = .88) showed good agreements. The PIT (Sn = .65, Sp = .63) and SIT validities (Sn = .68, Sp = .70) were compared with RF, showing a significant correlation in PIT and RF (r = .69), SIT and RF (r = .73), and PIT and K-ODI (r = .53). CONCLUSION: The SIT is a more comfortable position test than the PIT in patients. Both PIT and SIT have acceptable reliability and validity.

Ray Parenchyma and Ray Tracheid Structure of Four Korean Pine Wood Species

  • Ahmed, Sheikh Ali;Chong, Song-Ho;Chun, Su-Kyoung;Park, Byung-Su
    • Journal of the Korea Furniture Society
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    • v.17 no.4
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    • pp.101-107
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    • 2006
  • To know the structural difference between the ray parenchyma and ray tracheid among Pinus densiflora, Pinus rigida, Pinus koraiensis and Larix kaempferi, an observation was carried out under the FE-SEM. The longest ray parenchyma and ray tracheid were found in Pinus koraiensis species while the shortest ray tracheid and ray parenchyma were found in Pinus densiflora and Larix kaempferi. Larix kaempferi had more than one endwall pit in its ray parenchyma. Pinus densiflora was found highest in the pit aperture diameter in ray tracheid and aperture diameter in the cross-field pit. The pit border width in ray tracheid and lumen diameter of ray parenchyma were found highest in Pinus rigida. The cell wall thickness of ray parenchyma and pit aperture diameter in endwall pit of ray tracheid were found highest in Pinus koraiensis compared to other species.

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Application of Multiple Linear Regression to Predict Mechanical Properties of 316L Stainless Steel with Unspecified Pit Corrosion (불특정 공식손상을 가진 316L 스테인리스강의 기계적 물성치 예측을 위한 다중선형회귀 적용)

  • Kwang-Hu Jung;Seong-Jong Kim
    • Corrosion Science and Technology
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    • v.22 no.1
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    • pp.55-63
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    • 2023
  • The aim of this study was to propose a multiple linear regression (MLR) equation to predict ultimate tensile strength (UTS) of 316L stainless steel with unspecified pit corrosion. Tensile specimens with pit corrosion were prepared using a potentiostatic acceleration test method. Pit corrosion was characterized by measuring ten factors using a confocal laser microscope. Data were collected from 22 tensile tests. At 85% confidence level, total pit volume, maximum pit depth, mean ratio of surface area, and mean area were significant factors showing linear relationships with UTS. The MLR equation using these three significant factors at a 85% confidence level showed considerable prediction performance for UTS. Determination coefficient (R2) was 0.903 with training and test data sets. The yield strength ratio of 316L stainless steel was found to be around 0.85. All specimens with a pit corrosion presented a yield ratio of approximately 0.85 with R2 of 0.998. Therefore, pit corrosion did not affect the yield ratio.