• 한국생물공학회:학술대회논문집
• /
• 2000.04a
• /
• pp.235-238
• /
• 2000

The production of polysaccharide from Paecilomyces japonica was studied in the shake flask culture. For the cell growth and the polysaccharide production, the optimum synthetic medium was glucose peptone(YMP) medium. The flask culture conditions for the polysaccharide production were $27^{\circ}C$ and 200rpm with the initial pH 9 for 8days cultivation.

• KSBB Journal
• /
• v.13 no.3
• /
• pp.303-307
• /
• 1998

The fermentation conditions for the maximal production of the useful polysaccharides(water soluble polysaccharide and cell bound polysaccharide) from marine bacterium Zoogloea sp.(KCCM 10036) were investigated with a 5 L jar fermentor. The maximal production of these polysaccharides was obtained under the conditions of initial pH 7.8, 30$^{\circ}C$, 400 rpm of agitation speed, 2 Wm of aeration rate, 10%(w/v) of inoculum size and 2.5%(w/v) of glucose substrate and 10.38 g/L of total polysaccharide was produced. Apparent viscosity of the culture broth was increased with the production of these polysaccharides and the maximum value was reached to 22,500 cp.

• KSBB Journal
• /
• v.13 no.5
• /
• pp.547-553
• /
• 1998

For the efficient production of a new exo-polysaccharide from Ganoderma lucidum ASI 7004, the optimum conditions and methods in submerged cultivation were investigated with an airlift fermenter system. The optimum aeration rate was 2.5 Wm at the initial pH 5.0 and 28$^{\circ}C$. The increase of dissolved oxygen concentration by pure oxygen supply during cultivation did not improved the exo-polysaccaride production and the mycelial growth. The maximum exo-polysaccharide production and the mycelial growth under the optimum culture condition were obtained in media of glucose 60g/L, yeast extract 6g/L, (NH4)2HPO4 1g/L and KH2PO4 0.5g/L. Under these optimum medium and culture conditions, about 7.15g/L of exo-polysaccharide and 13.9g/L of mycelial growth were producted, respectively.

• The Korean Journal of Food And Nutrition
• /
• v.11 no.4
• /
• pp.416-419
• /
• 1998

Identification of escherchia coli K1 polysaccharide antigen isolated from urine specimens of urinary tract infections in children were performed from of 1992 to 1993 in Kyoto, Japan. The serotypes of E. coli were categorized that O1:H7, O2:H6, O2:H7, O16:H6, O18:H7, O18:H￣, and O135:H44 among 14 strains isolated from urine specimens of urinary tract infections in children by the serological test. And, one strain (O18:H￣, isolation rate: 7.1%) of E. coli K1 polysaccharide antigen among 14 strains were isolated from urine specimens of urinary tract infections in children by the bacteriophage test.

• Applied Biological Chemistry
• /
• v.41 no.4
• /
• pp.209-212
• /
• 1998

Methylan, a polysaccharide produced from Methylobacterium organophilum, was chemically modified by adding diethylaminoethyl (DEAE) group to the backbone of methylan. The structure of DEAE-methylan was determined by measuring its nitrogen content obtained from an elemental analysis. From the analysis of mass spectrum, the DEAE group in DEAE-methylan was also confirmed by determining diethylaminoethene as a separate form of DEAE. Mercury adsorption of DEAE-methylan was higher than that of native methylan. This fact was valid for a variety of pH, reaction times, metal concentrations, and polysaccharide concentrations. In particular, native methylan and DEAE-methylan adsorbed 16% (w/w) and 18% (w/w) for mercury after 30 min at pH 7, respectively. The increase in mercury adsorption of DEAE-methylan may be resulted from mercury adsorption by the lone pair electron of nitrogen atom in DEAE group.

• Journal of Ginseng Research
• /
• v.21 no.2
• /
• pp.78-84
• /
• 1997

As a part of our ongoing effort to develop new antineoplastic immunostimulator from natural sources, bioassay-directed fractionationn of polysaccharides from Korean red ginseng was carried out by observing the proliferation of marine spleen cells and the generation of lymphoklne activated killer (LAK) cells. The acidic polysaccharide fractions proliferated spleen cells and generated LAK cells in proportion to their acidity in vitro. The LAK cell which was induced by ginseng showed tumoricidal activity against both NK celt sensitive and insensitive tumor target cells without major histocompatibility (MHC) restriction. Adherent macrophages and CD4+helper T cells were involved in the generation of the LAK cells. The acidic polysaccharide from Korean red ginseng synerglzed with recombinant IL-2 (rIff-2) at lower than 3 U/ml. The optimal doses of the acidic polysaccharide from Korean red ginseng for the proliferation of spleen cells and for the generation of LAK cells were 1 mg/ml and 100 $\mu\textrm{g}$/ml, respectively; this means that the mechanisms for the both activities may be different from each other.

• 한국생물공학회:학술대회논문집
• /
• 2000.11a
• /
• pp.283-286
• /
• 2000

In order to maximize the cell growth and the polysaccharide production in Agaricus blazei, two kinds of fed-batch fermentation processes were performed with varying the feeding medium compositions and the feeding process. The relationship between dissolved oxygen and polysaccharide production in batch fermentation was applied to fed-batch fermentation. The biomasss concentration was 18.2 g/L and the polysaccharide production was 10.4 g/L.

• Journal of Marine Bioscience and Biotechnology
• /
• v.9 no.2
• /
• pp.58-64
• /
• 2017

Polysaccharides are useful materials for keep skin moisturizing and skin improvement cosmetic materials. We have isolated polysaccharides form Gracilariopsis sp. The polysaccharide derived from Gracilariopsis sp. have various size of 300kDa, 500kDa and over 1,000kDa. The volunteers applied a cream formula with polysaccharides from Graciariopsis sp. for 4 weeks. We found significant improvement of wrinkles and skin texture. We also found an effect of improving skin elasticity. When the moisture reduction rate of the skin was 2.8% lower than the 5.1% of hyaluronic acid. The polysaccharide extract from Gracilariopsis sp. showed stable pH and viscosity. The polysaccharides derived from Gracilariopsis sp. could be used as a cosmetic material.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.12
• /
• pp.1890-1894
• /
• 2008

Apoemulsan is a biopolymer with potent emulsification activity, produced by Acinetobacter venetian us RAG-1 (RAG-1). The wee gene cluster is responsible for apoemulsan biosynthesis. The analysis of (i) a putative polysaccharide copolymerase mutant (${\Delta}wzc$), (ii) a putative polymerase mutant (${\Delta}wzy$), and (iii) an apoemulsan-deficient variant (${\Delta}2$) indicated that the wee gene cluster controls the synthesis of two polysaccharides: high molecular weight (HMW) and low molecular weight (LMW). LMW polysaccharide of wee origin was present in LPS isolated from RAG-1 cells, suggesting a link to the Lipid A-core of LPS molecules. SDS-PAGE analysis indicated that apoemulsan is copurified with LPS polysaccharide, with implications in the emulsification activity of RAG-1 polymer.

• Journal of Ginseng Research
• /
• v.25 no.2
• /
• pp.63-67
• /
• 2001

We previously reported that acidic polysaccharide from Panax ginseng induced the proliferation lymphocytes and the generation of activated killer cells. Here we found that polysaccharide (PG-75) precipitated with 75% EtOH from water extract of Panax ginseng also has both in vitron and in vivo hematopoietic activities. In vitro studied with bone marrow cells from BALB/c mouse revealed that PG-75 had direct effect on hematopoietic colony-forming cell(CFC) growth, increased granulocyte macrophage-colony forming cell numbers by 1.59 fold over than non-treated. the ability of PG-75 to modulate hematopoiesis in vivo was evaluated the bone marrow and spleen celluarity, granulocyte-macrophage progenitor cells. BALB/c female mice were administered G-75 intraperitoneally, PG-75 was found to significantly increase the number of BM cells, spleen cells, GM-CFU on 3 hours after injection. PG-75 was also able to induce significant augmentation of GM-CSF and IFN-${\gamma}$, production in sera. These studies illustrate than PG-75 has hematopoietic activities and that this agent may be useful in the prevention and/or treatment of radio- or chemotherapy-associated myelosuppression.