• Title, Summary, Keyword: polysaccharide

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Efficient Immobilization of Polysaccharide Derivatives as Chiral Stationary Phases via Copolymerization with Vinyl Monomers

  • Chen, Xiaoming;Okamoto, Yoshio;Yamamoto, Chiyo
    • Macromolecular Research
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    • v.15 no.2
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    • pp.134-141
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    • 2007
  • The direct chromatographic separation of enantiomers by chiral stationary phases (CSPs) has been extensively developed over the past two decades, and has now become the most popular method for the analytical and preparative separations of enantiomers. Polysaccharide derivatives coated onto silica gel, as CSPs, playa significantly important role in the enantioseparations of a wide range of chiral compounds using high-performance liquid chromatography (HPLC). Unfortunately, the strict solvent limitation of the mobile phases is the main defect in the method developments of these types of coated CSPs. Therefore, the immobilization of polysaccharide derivatives onto silica gel, via chemical bonding, to obtain a new generation of CSPs compatible with the universal solvents used in HPLC is increasingly important. In this article, our recent studies on the immobilization of polysaccharide derivatives onto the silica gel, as CSPs, through radical copolymerization with various vinyl monomers are reported. Polysaccharide derivatives, with low vinyl content, can be efficiently fixed onto silica gel with high chiral recognition.

Polysaccharide from Polygonatum Inhibits the Proliferation of Prostate Cancer-Associated Fibroblasts Cells

  • Han, Shu-Yu;Hu, Ming-Hua;Qi, Guan-Yun;Ma, Chao-Xiong;Wang, Yuan-Yuan;Ma, Fang-Li;Tao, Ning;Qin, Zhi-Hai
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.8
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    • pp.3829-3833
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    • 2016
  • Inhibition of cancer-associated fibroblasts (CAFs) may improve the efficacy of cancer therapy. Polysaccharide extracted from polygonatum can selectively inhibit the growth of prostate-CAFs (p<0.001) without inhibiting the growth of normal fibroblasts (NAFs). Polysaccharides from polygonatum stimulate autophagy of prostate-CAFs. 3-methyl-adenine(3-MA) is an autophagy inhibitor. 3-MA was added to prostate-CAFs with polysaccharide from polygonatum to determine whether autophagy plays an important role in the restrained effect. Finally, polysaccharide from polygonatum treatment significantly increased the activation of Beclin-1 and LC3, key autophagy proteins. Polysaccharide from polygonatum stimulates autophagy of prostate-CAFs and inhibits prostate-CAF growth, indicating that a novel anti-cancer strategy involves inhibiting the growth of prostate-CAFs.

Effect of Culture Conditions on Production of Polysaccharides and Growth Rate of Porphyridium cruentum (Porphyridium cruentum의 성장 및 당질 생산에 미치는 배양 조건의 영향)

  • Joo, Dong-Sik;Choi, Soon-Yeong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.41 no.6
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    • pp.446-451
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    • 2008
  • The growth of Porphyridium cruentum and its porphyran polysaccharide production were measured as functions of light intensity, temperature, light quality (fluorescent, blue, red, and green) and nitrate concentration. The optimum light intensity, temperature, and nitrate concentration for the growth of Porphyridium cruentum and for its polysaccharide production were 1,400 lx, $25^{\circ}C$, and 0.03%, respectively. The maximum cell concentration and polysaccharide content under the optimum conditions were 1.95 and 0.23 mg/mL, respectively. Light quality did not influence growth or polysaccharide production. The best results for growth and polysaccharide production were obtained using fluorescent light.

Exo-Polysaccharide Production in Liquid Culture of Pleurotus ferulae

  • CHOI DU BOK;KANG SI HYUNG;SONG YON HO;KWUN KYU HYUK;JEONG KYOUNG JU;CHA WOL SUK
    • Journal of Microbiology and Biotechnology
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    • v.15 no.2
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    • pp.368-375
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    • 2005
  • Batch cultures were carried out to optimize the exo-polysaccharide production by liquid cultures of Pleurotus ferulae. Among the various carbon sources, when $5\%$ of glucose was used, the maximum mycelial growth and exo-polysaccharide concentration reached were 8.78 g/l and 3.59 g/l, respectively. Yeast extract and polypeptone were identified as the most suitable nitrogen sources. In particular, when a mixture of $1\%$ of polypeptone and $0.8\%$ of yeast extract was used, 9.52 g/l of mycelial growth and 4.09 g/l of exo-polysaccharide were obtained. In the case of mineral sources, K$_2$HPO$_4$ and MgSO$_4$$\codt$7H$_2$0 were found to be the best mineral sources for mycelial growth and exo-polysaccharide production. Under the optimized culture conditions, the agitation speed and aeration were investigated for mycelial growth and exo­polysaccharide production in a jar fermentor. The maximum mycelial growth and exo-polysaccharide concentration at 1.5 vvm and 200 rpm obtained were 13.2 g/l and 4.95 g/l, respectively, after 10 days of culture, which were $76\%$ and $79\%$ higher than those of the basal medium. The specific growth rate was decreased with the increase of mycelial growth. However, the specific production rate of the exo-polysaccharide was proportionally increased with the specific growth rate. The proposed model profiles showed good agreement with the experimental results for the mycelial growth and exo-polysaccharide production. The specific production rate using the optimized medium was higher than that of basal medium.

Effect of Korean Red Ginseng Polysaccharide on Lipolytic Action of Toxohormone-L from Cancerous Ascites Fluid (고려홍삼 다당체 성분이 암독소 호르몬-L의 체지방 분해작용에 미치는 영향)

  • 이성동;신유정
    • The Korean Journal of Food And Nutrition
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    • v.8 no.2
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    • pp.110-115
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    • 1995
  • This study was devised to observe the inhibitory effects of ethanol treatment precipitate (crude acidic polysaccharide) from Korean red ginseng on a lipolytic action of Toxohormone-L which has been known as lipolytic and anorexigenic factors. Toxohormone-L was obtained by partial purification of the ascites fluid from mice which had been inoculated with sarcoma-180. The yields of crude acidic polysaccharide from Korean red ginseng was 63.5%. In vitro, at the concentration of 500rg /ml, the inhibition rate of lipolysis by the crude acidic polysaccharide of Korean red ginseng was 38.8% and the total inhibitory activity per gram of ginseng material was 4,928 nit. In vivo, the red ginseng polysaccharide(40mg/ml in saline soon.) 16ul/g of body weight was injected to the sarcoma-180 bearing mice once In 3 days until death. The effects against the extension of life span was little but body weight gain of sarcoma-180 bearing mice decreased significantly by administration of Korean red ginseng polysaccharide compared to those of the control group.

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Polymer-Coated Liposomes for Oral Drug Delivery (I): Stability of Polysaccharide-Coated Liposomes Against Bile Salts (고분자 코팅을 이용한 경구용 리포좀의 개발(I): 다당체로 코팅된 리포좀의 담즙산염에 대한 안정성)

  • Choi, Young-Wook;Hahn, Yang-Hee
    • Journal of Pharmaceutical Investigation
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    • v.22 no.3
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    • pp.211-217
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    • 1992
  • Stabilization of liposomes against degradation by bile salts has been investigated in order to develop a liposomal model system for oral drug delivery. Two polysaccharides, amylopectin (AP) and chitin (CT), were employed to coat both empty liposomes and bromthymol blue (BTB)-encapsulated liposomes by adsorption-coating techniques. Turbidity changes and BTB-release characteristics in pH 5.6 buffer solutions with or without bile salts, sodium cholate and sodium glycocholate, were observed to compare the differences between uncoated liposomes and polysaccharide-coated liposomes. Initial turbidities of both uncoated and polysaccharide-coated liposomes in buffer solution were kept constant within 3% range during 4 hours of experiments. But they were decreased in a different manner in bile salts-containing buffer solutions, showing 10% or less decrease for polysaccharide-coated liposomes and 25% or more decrease for uncoated liposomes. BTB release from uncoated liposomes has been greatly increased upto 90% after 4 hours in bile salts-containing buffer solution, which is a clue for breakdown of liposomal vesicles. However, polysaccharide-coated liposomes showed the controlled-release pattern which is proportional to square-root of time, followed by around 50% release for the same time period. Consequently, it is possible to conclude that these polysaccharide-coated liposomes might be an available system for oral delivery of a drug which is unstable in gut environment.

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Anti-inflammatory Agents from Animals(II) - Anti-inflammatory, Analgesic and Immunoregulatory Activities of Mylabris sidae and Epicauta gorhami Polysaccharide Fractions - (동물성 소염진통제 (II) - 반묘 및 먹가래 다당체분획의 소염.진통 및 면역조절작용 -)

  • 김창종;최충식;조승길
    • YAKHAK HOEJI
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    • v.35 no.5
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    • pp.360-367
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    • 1991
  • Effect of Mylabris sidae(MS) and Epicauta gorhami(EG) polysaccharide fractions on the inflammation and immune responses were studied in vivo. MS and EG contained cantharidin about 0.61 and 0.65%, respectively. It was shown that MS and EG polysaccharide fractions at a oral dose of 100 mg/kg have the significant anti-inflammatory and analgesic activity; They inhibited significantly the carrageenin-induced inflammation and acetic acid-induced writhing syndrome. They accelerated significantly the carbon clearance and the phagocytosis of colloidal carbons by Kupffer cells in liver, but they at a oral dose of 100 mg/kg suppressed significantly the Arthus reaction in the sheep red blood cell(S-RBC)-sensitized mice in accordance with the inhibition of haemaglutinin titer, haemolysin titer and plaque-forming cells. On the other hand, they at a oral dose of 200 mg/kg accelerated slightly the oxazolone-induced dermatitis in rats and delayed hypersensitivity in the S-RBC-challenzed mice in consistent with the increase of rosette forming cells. As the above results, it exhibited that MS and EG polysaccharide fractions inhibited the humoral immune responses, but they accelerated the function of macrophages and cellular immune responses. EG polysaccharide fraction had more active than MS polysaccharide fraction.

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Separating and purifying polysaccharide, polypeptide from sea cucumber and their functions (해삼으로부터 다당류와 폴리펩타이드의 분리, 정제 및 기능성)

  • Jin, Hai-zhu;Fu, Xue-jun;Shen, Jing-yu;Sun, Bo;Wang, Hong-tao
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • pp.112-123
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    • 2005
  • For thestudying and utilizing the thorn sea cucumber synthetically, the method preparing polysaccharide and polypeptide from sea cucumber was studied. The comparatively rational craft for preparing crude polysaccharide is fresh raw materials deal with oar form, $60\%$ of the ethanol precipitate after hydrolyzed by pretense and vibrated with ultrasonic wave auxiliary. The purification of polysaccharide and removal of protein fromcrude polysaccharide were made through precipitation method using the acetate. The polypeptide is obtained by concentrating in vacuum, freeze-drying after mixing precipitations of two times. Polysaccharide acute poisoning experiment indicate there is no bad reaction when LD50>5000g/kg, there strain rate of liver tumor H11 approach present generally acknowledged tumor treat medicine cyclophosphamide, and there is no side effect. The fatigue resistance function of polypeptide experiment also indicates that the fatigue resistance ability of mouse which fed on added sea cucumber polypeptide has a great improvement.

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Optimization of Extraction Conditions for Polysaccharide using Red Ginseng Marc (홍삼박을 이용한 다당체 추출조건 최적화)

  • Tark, Keun-Man;Cho, Kyung-Lae;Park, Keun-Hyoung;Son, Seok-Min;Chae, Hee-Jeong
    • Journal of Ginseng Research
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    • v.33 no.4
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    • pp.337-342
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    • 2009
  • The extraction conditions for the production of red ginseng polysaccharide were proposed. The crude fiber content of red ginseng marc (RGM) (15.3%) was much higher than that of white ginseng (WG) (2.1%) and red ginseng (RG) (0.5%). Thus, RGM was selected as the raw material for polysaccharide production. The correlation between the particle size of RGM and the polysaccharide extraction was investigated with a correlation analysis using the SPSS package. The two parameters were found to have a significant correlation (p<0.01). The polysaccharide extraction increased as the particle size of RGM decreased. The optimal concentration of RGM was 6.66% (w/v). The extraction yield increased as both the extraction temperature and the extraction time increased. Finally, the extraction temperature and time were selected as $85^{\circ}C$ and 5 hrs, respectively. Consequently, the extraction conditions for polysaccharide production were optimized and statistically confirmed.

Purification and Partial Characterization of an Acidic Polysaccharide with Complement Fixing Ability from the Stems of Avicennia Marina

  • Fang, Xubo;Jiang, Bo;Wang, Xiaolan
    • BMB Reports
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    • v.39 no.5
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    • pp.546-555
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    • 2006
  • An acidic polysaccharide fraction that had high anti-complementary activity was isolated from the stems of Grey Mangrove in 0.15% yield. The final fractions was designated HAM-3-IIb-II. The polysaccharide fraction appeared to be homogenous by high performance size exclusion chromatography with an estimated molecular weight of 105 kDa. The isolated polysaccharide is more effective than polysaccharide K (PSK) in its anti-complementary activity at 58 ${\mu}g/ml$ of PSK and 23 ${\mu}g/ml$ of HAM-3-IIb-II that inhibit 50% of complement activity in the complement fixation assay. Structural studies indicated that HAM-3-IIb-II was rich in galacturonic acid along with arabinose, galactose and rhamnose, characterizing a pectin-type polysaccharide, which was also confirmed by FT-IR spectrum. The presence of rich neutral sugar side chains of arabinogalactans may have contributed to the expression of high activity. Traditionally, this mangrove plant is used for medicinal purposes and it appears to have some scientific applications.