• Korean Journal of Pharmacognosy
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• v.41 no.3
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• pp.210-215
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• 2010

Acute toxicity on the polysaccharide fraction of Pueraria lobata was examined using male and female Sprague-Dawley rats. The polysaccharide fraction of Pueraria lobata was orally administered at a dose of 5 mg/kg, 50 mg/kg, 500 mg/kg, 2,000 mg/kg and 5,000 mg/kg and observed for two weeks. No mortality and abnormal clinical signs were observed at the doses used. There were not any significant differences in parameters of blood biochemical values and urinalysis by the treatment of test material. All rats were appeared to be healthy and normal throughout the observation period. Also there was no difference in net body weight gain and gross pathological findings among the groups rats treated with different doses of the polysaccharide fraction with Pueraria lobata.

• The Korean Journal of Food And Nutrition
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• v.32 no.5
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• pp.417-424
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• 2019

The purpose of this study was to observe the effects of the polysaccharide (GLP) obtained from the liquid cultured Ganoderma lucidum on the lipidperoxidation in a rat liver microsome and hepatotoxicity in the primary cultured rat hepatocytes. It is well known that the polysaccharide of Ganoderma lucidum exhibits hepatoprotective activity, antitumor activity etc., which many suggest a relationship to lipidperoxidation. The effect of GLP on $CCl_4-$ and galactosamineintoxicated cytotoxicity in the primary cultured rat hepatocytes were reduced the GPT value. In order to the estimate the effects of anti-lipidperoxidation of the polysaccharide, enzymatic and nonenzymatic reaction assays were performed, in vitro, in the rat liver microsome. An enzymatic lipidperoxidation reaction by $ADP+FeCl_3+NADPH$ and $CCl_4+NADPH$, GLP (1 mg/mL) inhibited 77.4% and 39.4%, respectively, and the nonenzymatic reaction displayed a 97.4% strongly inhibition. In the enzymatic and nonenzymatic inducers treated with GLP, the formation of malondialdehyde (MDA) progressively decreased by raising the GLP concentration. These results suggest that the anti-lipidperoxidation and radical scavenging activity of GLP may play an important part in the liver protection action.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.502-505
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• 2005

This study was concentrated to investigate the dependency of recovery yield on the extraction conditions. From fruiting body, the extracted amount of polysaccharide was studied with various solvent. In order to maximize the yield, the optimum extraction conditions were elucidated with respect to solvent, temperature and time. In addition, the dialysis was applied to obtain the higher purity of polysaccharide with varying the exposure time.

• Journal of Ginseng Research
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• v.17 no.2
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• pp.145-147
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• 1993

The amounts of ginseng acidic polysaccharide (GAP) in red ginseng (Panax ginseng) were higher than those of wild and cultured Panax quinquefolius, Panax notoginseng as well as white ginseng (Panax ginseng). In white ginseng, there is no difference in the GAP amount among root ages or sizes. Also, the GAP amount of red ginseng body was similar to that of ginseng rhizome, but was higher than that of leaf and epidermis.

• Archives of Pharmacal Research
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• v.14 no.1
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• pp.68-72
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• 1991

The polysaccharide fraction from Taraxii Herba showed potent immunopotentiating activities with antitumor activities. The fraction having small amount of protein inhibited the growth of solid tumor and increased peritoneal exudate cells and immunoorgan weights in normal mice, and also increased hypersensitivities in tumor bearing mice.

• YAKHAK HOEJI
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• v.43 no.5
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• pp.629-634
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• 1999

A protein-polysaccharide fraction was isolated from the mycelial culture of a Korean basidiomycetous fungus, Hebeloma crustuliniforme, and was named HCA (Hebeloma crustulineforme fraction A). When intraperitoneally injected at 200 mg/kg/day once daily for 10 days, HCA inhibited the growth of sarcoma 180 solid tumor in ICR mice by 65.1% (p＜0.05). HCA also exerted protective effect against leukopenia induced by cyclophosphamide with the protection ratio of 39.7% (p＜0.01).

• The Korean Journal of Mycology
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• v.11 no.4
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• pp.159-162
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• 1983

The dried carpophores of Sarcodon aspratus were examined for mineral elements and a protein-polysacccharide. Calcium, iron, zinc, magnesium, manganese, copper, lead, cadmium and mercury were detected in that order of contents by atomic absorption spectrometry and automatic mercury analysis. The protein-polysaccharide fraction extracted from the carpophore was found to contain 31.5% protein and 52.8% polysaccharide. The fraction showed no antitumor activity against sarcoma 180 implanted in mice.

• Archives of Pharmacal Research
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• v.13 no.3
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• pp.285-288
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• 1990

The polysaccharide fraction from the rhizome of Trichosanthes kirilowii (Cu-curbitaceae) showed marked antitumor and cytotoxic activity with immunopotentiating activity. It was evidenced by the increase in the number of circulating leucocytes and peritoneal exudate cells and the recovery of lowered antibody forming activity in mice. The polysaccharide was mainly composed of glucose, galactose, fructose, manmose and xylose and a small amount of protein.

• Fisheries and Aquatic Sciences
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• v.9 no.3
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• pp.115-117
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• 2006

A micro algal growth-enhancing polysaccharide compound was isolated from the green alga Monostroma nitidum using water extraction, molecular fractionation, a DEAE-cellulose column, and fast protein liquid chromatography using a Superose-12 column. The yield of the compound from the seaweed powder was 8.3$\times$l0$^{-3}$%. At 2 mg/mL concentration, the polysaccharide enhanced Tetraselmis suecica cell growth in f/2 medium by approximately 160%.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.325-327
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• 2000

We developed a on-line gas control system for three gas components and investigated the effect of oxygen, carbon dioxide, and ethylene on polysaccharide production and cell growth in the suspension cultures of Aloe saponaria. Carbon dioxide(5% v/v) enhanced significantly polysaccharide production while the cell growth was not affected.