• Journal of Plant Biotechnology
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• 제32권3호
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• pp.217-223
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• 2005

액체배양을 통한 부채마의 부정근 배양시 첨가되는 생장 조절제의 종류와 농도에 따른 부정근의 생장과 steroidal saponin 생산과의 관계를 조사하였다. NAA와 BA 농도별 부정근 생장은 NAA를 단독으로 1.0 mg/L를 처리한 곳에서 가장 효과적이었으나 NAA에 BA를 첨가하면 부정근의 생장에 오히려 억제효과를 보였다. 20종의 각각 다른 배양주로부터 dioscin, prosapogenin A 및 prosapogenin C의 함량을 조사한 결과 배양주에 따라 큰 차이를 보였는데, 그 중 dioscin 함량이 건물당 2.5%로 가장 높았던 10번 배양주를 선발하였다. 부정근에 함유된 세 종류의 steroidal saponin 함량은 부정근의 생장이 왕성한 4주까지는 낮으나 부정근의 생장이 거의 정지되는 5주째에 급격히 증가하였다. Dioscin과 prosapogenin C의 함량은 NAA보다 IBA를 처리한곳에서 현저히 높으나 prosapogenin A의 함량은 이들 두성분의 종류와 농도에 영향을 받지 않았다.

• 생약학회지
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• 제9권2호
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• pp.93-97
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• 1978

From the crude saponin obtained from the pods of Gleditschia officinalis seven spots were identified by TLC and quantity of gleditschia B was the highest among them. Ten kinds of prosapogenins were identified from the partial hydrolysates of crude saponin and found that prosapogenin E contain oleanolic acid as a sapogenin and prosapogenin F does echinocystic acid as the sapogenin. Hydrolysis of crude saponin yield glucose and rhamnose and the same sugar were also identified from the mixture of prosapogenin E and F.

• 생약학회지
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• 제45권4호
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• pp.315-319
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• 2014

This study compared the contents of ginseng prosapogenin depending on the extracting conditions of Skin White ginseng(Panax ginseng) to provide basic information for developing Skin White ginseng-based functional foods. Our findings show that the content of crude saponin peaked at 4 hours of extraction(SWG-4) and when extracted at $100^{\circ}C$. However, the content of total saponin reached its height at 8 hours of extraction at $100^{\circ}C$(SWG-8). On the other hand, the content of prosapogenin reached their heights at 60 hours of extraction(SWG-60), followed by 60 hours of extraction at $100^{\circ}C$. And at $100^{\circ}C$ the main prosapogenin of the content of Red and Black ginseng ginsenoside $Rg_3$, $Rg_5$ and $Rk_1$ reached their heights at 60 hours of extraction(SWG-60), followed by 60 hours of extraction.

• Journal of Ginseng Research
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• 제9권2호
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• pp.232-239
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• 1985

The effects of total saponin, triol and diol saponin, and prosapogenin of ginseng on the absorption of cholesterol and the component of serum lipids was observed as a function of time using isotopic method. The following results were obtained. 1) Labelled cholesterol absorption was observed to be enhanced after adminstrations of total saponin, triol saponin, and. prosapogenin of ginseng to rat (Sprague Dawley). 2) Decreases in the amount of serum lipid were observed after administration of total saponin and prosapogenin of ginseng.

• Journal of Ginseng Research
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• 제40권1호
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• pp.86-89
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• 2016

This study compared the contents of prosapogenin depending on the extracting conditions of Red ginseng to provide basic information for developing Red ginseng-based functional foods. The content of ginsenoside Rg3 reached their maximum value at 24 h of extraction, followed by 36 h and 72 h of extraction at $100^{\circ}C$.

• 생약학회지
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• 제42권4호
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• pp.361-365
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• 2011

The objective of this study is to provide basic information for developing a high-value ginseng product using ginseng saponin and prosapogenin. In order to achieve the proposed objective ginsenoside compositions of Black (BG) and Red (RG) ginseng extract with 95% ethyl alcohol were examined by means of HPLC. The crude saponin and ginsenoside composition of processed ginseng products were analyzed and compared, with BG topping the list with a crude saponin content of 7.53%, followed by RG (5.29%). Ginseng prosapogenin (ginsenosides $Rg_2$, $Rg_3$, $Rg_5$, $Rg_6$, $Rh_1$, $Rh_4$, $Rk_1$, $Rk_3$, $F_1$ and $F_4$) in BG was found to be contained almost 2.6 times as much as that in RG. Ginsenosides $Rg_3$, $Rg_5$, $Rk_1$, $Rh_4$ and $F_4$ in BG in particular were found to be almost 3 times as much as those in RG. $Rg_6$ and $Rk_3$ in BG were also found to be almost 4 times as much as those in RG.

• 약학회지
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• 제49권6호
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• pp.490-494
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• 2005

The objective of this study is to provide the basic information for developing a high-value ginseng product using ginseng saponin and prosapogenin. In order to achieve such aim, Ginsenoside compositions of black ginseng (BG) extracts with various solvent conditions were examined by HPLC. The total saponin and the prosapogenin content of 95$\%$ ethyl alcohol extract were higher than that of the either 50$\%$ ethyl alcohol extract or distilled water extract. As a result, the order of the total saponin and the prosapogenin content was 1) 95$\%$ ethyl alcohol,2) 50$\%$ ethyl alcohol,3) the first and second mixture of 95$\%$ ethyl alcohol, distilled water, and 4) distilled water extract. In the case of fine black ginseng (FBG), the first and second mixture extracts of 95$\%$ ethyl alcohol and distilled water were the highest. In addition, the ratio of the protopanaxadiol group and the protopanaxatriol group (PD/PT) showed that the ratio of BG ranged from 0.304 to 0.601, while the ratio of FBG ranged from 1.166 to 1.657.

• Journal of Ginseng Research
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• 제15권3호
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• pp.188-191
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• 1991

The mild hydrolysis of ginsenosie Re with 50% acetic acid gave a prosapogenin mixture, 20(R)- and 20(S)-ginsenoside Rg2. The products were acetylated to give the peracetates, which were further converted into 20(R)- and 20(S)-ginsenoside Rh1 by the alcoholic alkaline treatment.

• 생약학회지
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• 제22권2호
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• pp.73-77
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• 1991

Further studies have been done on the constituents of the tubers of Liriope spicata $L_{OUR}$ (Liliaceae). Four steroidal glycosides, tentatively designated as compounds I. II, III and IV, were isolated from the n-BuOH soluble fraction of this plant. The structures of these glycosides were established as ${\beta}-sitosterol$ glucoside, prosapogenin II of spicatoside A, ophiopogonin B, and prosapogenin III of spicatoside A.

• 생약학회지
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• 제20권2호
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• pp.76-82
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• 1989

Five known diosgenin glycosides have been isolated from the MeOH extract of Smilax china rhizomes and characterized as prosapogenin A of dioscin, dioscin, gracillin, methyl protogracillin, methyl protodioscin and its corresponding 22-hydroxy analog. This is the first isolation of the former four compounds from this plant. ${\beta}-Sitosterol\;glucoside$ was also isolated and identified.