• 제목/요약/키워드: protein kinase C

검색결과 452건 처리시간 0.136초

세포신호계에 있어서 Protein Kinase C: 사람의 전입선 adenocarcinoma PC-3 세포내의 여섯개의 Protein kinase C 동립효소의 translocation (Protein Kinase C (PKC) in Cellular Signalling System: Translocation of Six Protein Kinase C Isozymes in Human Prostate Adenocarcinoma PC-3 Cell Line)

  • Park, Won-Chul;Ahn, Chang-Ho
    • 한국동물학회지
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    • 제36권4호
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    • pp.439-451
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    • 1993
  • Protein kinase C isozymes in a human prostate adenocarcinoma PC-3 cell line were characterized. Immunoreactive bands and immunocytochemical stains were obsenred in PC-3 cells with antibodies raised against protein kinase C ${\alpha}$, ${\beta}$, ${\gamma}$, $\delta$, $\varepsilon$, and ζ types, respectively. Protein kinase C ${\alpha}$ corresponded to a immunoreactive band at a molecular weight of 80,000-dalton, whereas molecular weights of other immunoreactive isozvmes of protein kinase C were detected at 68,000-dalton. Protein kinHse C $\delta$ and ζ antibodies detected additional bands at 55,000-dalton and 80,000-dalton, respectively Immunocvtochemical study confirmed the results of the immunoblotting experiments qualitatively: all six protein kinase C isozymes were detected in the cytoplasm of PC-3 cells. Translocation of protein kinase C in PC-3 cells were also examined with phorbol 12-myristate 13-acetate (PMA), bryostatin 2, diolein, and 1-oleoyl-2-acetyl glycerol (OAG). Differential reactions of protein kinase C isozvmes to these activators were obsenred. When PC-3 cells were treated with 10mM bryostatin 2, protein kinase C isozyme u was translocated into the nucleus, whereas s type was translocated into the plasma membrane and the nucleus. Protein kinase C ${\alpha}$ and ζ types were translocated into the nucleus following the treatment with 101M diolein, whereas protein kinase C ${\alpha}$, ${\beta}$, ${\gamma}$, and $\varepsilon$ types were translocated into the nucleus by the treatment with 10mM OAG. Protein kinase C ${\alpha}$ and $\varepsilon$ types were translocated into the nucleus in the presence of 100nM PMA. Protein kinase C $\delta$ type was translocated to the nuclear membrane by these activators, however, only PMA-induced translocation was inhibited by protein kinase C inhibitor, 1-(5-isoquinolinesulfonyll-2-methvlpiperazine dihvdrochloride (H7) . H7 inhibited translocation of protein kinase C ${\alpha}$ type induced by PMA, ${\beta}$ type by OAG and s type by PMA and OAG, whereas it did not affect translocations induced by bryostatin and diolein, respectively. These results suggest that there exist six isoformes of protein kinase C (${\alpha}$, ${\beta}$, ${\gamma}$, $\delta$, $\varepsilon$ and ζ types) in PC-3 cells and that each of these isozvmes distinctivelv reacts to bryostatin, diolein, OAG and PMA, in part due to an altered molecular size and conceivably discrete binding site(s).

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능소화의 꽃받침으로부터 Protein Kinase C 저해물질인 Verbascoside의 분리 및 그 생물활성 (Isolation and Biological Activity of Verbascoside, A Potent Inhibitor of Protein Kinase C from the Calyx of Campsis grandiflora)

  • 이현선;박문수;오원근;안순철;김보연;김환묵;오구택;민태익;안종석
    • 약학회지
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    • 제37권6호
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    • pp.598-604
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    • 1993
  • The calyx extract of Campsis grandiflora displayed inhibitory activity against protein kinase C from the bovine brain. Separation guided by protein kinase C enzyme assay and bleb forming assay led to isolation of a potent protein kinase C inhibitor that was identified as a known phenylpropanoid glycoside, verbascoside. It suppressed completely bleb-formation of K562 cell surface induced by phorbol 12,13-dibutylate at the concentration of 60 $\mu\textrm{g}$/ml and IC$_{50}$ of the protein kinase C occured at 20 $\mu{M}$. This compound was tested for cytotoxic activity against ten human tumor cell lines in vitro. it exhibited moderate cytotoxic activity against skin tumor cell line M14 (IC$_{50}$ 2.2 $\mu\textrm{g}$/ml) and very weak cytotoxicity against other cell lines (IC$_{50}$>10 $\mu\textrm{g}$/ml)

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고지방식이와 열량제한식이가 백서상피세포의 Protein Kinase C 활성에 미치는 영향 (Effect of High Fat Diet and Calorie-restricted Diet on Protein Kinase C Activity in Mouse Epidermal Cell)

  • 최면
    • Journal of Nutrition and Health
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    • 제24권3호
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    • pp.149-156
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    • 1991
  • 고지방식이와 총열량섭취 제한식이가 protein kinase C 활성에 미치는 영향을 규명하고자 기타 양양소 또 총열량섭취가 정확이 조절된 식이를 제조하여 투여해본 결과, protein kinase C의 활성은 본 실험에서 사용한 옥수수유의 양의 증가에 따라 cytosolic과 membrane-associated protein kinase C의 활성을 모두 증가시키는 것을 관찰 하였다. 또한 기타 영양소는 같게 투여하고 열량소만 40% 감소시킨 실험군은 대조군에 비해 양쪽의 protein kinase C가 모두 감소하는 경향을 보였다.

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Molecular cloning and nucleotide sequence of schizosaccharomyces pombe Homologue of the receptor for activated protein kinase C gene

  • Park, Seung-Keil;Yoo, Hyang-Sook
    • Journal of Microbiology
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    • 제33권2호
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    • pp.128-131
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    • 1995
  • Using differential hybridization, we selected the prk gene fortuitously from Schizosaccharomyces pombe homologous to RACK1 of rat which encodes the receptor for activated protein kinase C. The cDNA sequence of prk was determined and its deduced amino acid sequence was 76% homologous to RACK1 and had the feature of trimeric G protein bata subunit. The specific amino acid sequences required for the protein kinase C binding were also present in Prk as in the case of RACK1 protein. From these similarities, we suggest that the Prk is protein kinase C binding protein of S. prombe. The involvement of Prk in signal transduction mediated by protein kinase C remained to be studied.

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인삼 Petroleum-ether 추출물이 종양세포의 증식 주기 진행 및 Protein Kinase C의 활성에 미치는 영향 (Effects of the Petroleum-ether Extract of Ginseng on the Cell Cycle and Protein Kinase C Activity in Cancer Cells)

  • 박민경;황우익
    • Journal of Ginseng Research
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    • 제20권3호
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    • pp.219-225
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    • 1996
  • This study was performed to investigate the inhibition mechanism of cancer cell proof iferation caused by the petroleum-ether extract of ginseng against human rectum (HRT-18), colon (HT-29), llepatoma (Hep G2) and prostate (LNCaP) cancer cells and monkey kidney cells (Vero 76). Cells were treated with the petroleum-ether extract of ginseng (50 to 200 $\mu\textrm{g}$/ml) in G1 or S phase of the cell cycle, and proliferation and protein kinase C activity were measured. The petroleum-eth or extract of ginseng inhibited proliferation of HRT-18, HT-29, Hep G2 and LNCaP when treated in Gl phase, but not in S phase. This result shows that the ginseng extract arrests the cell cycle in G1 phase, resulting in the inhibition of cell proliferation. At the same concentrations, treatment of the ginseng extract in G1 phase decreased protein kinase C activity, while the treatment in S phase had no effect. This reault suggests that protein kinase C might be involved in the inhibition of the cell cycle and proliferation of cancer cells caused by the petroleum-ether extract of ginseng.

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Ca$^2+$ 및 Protein Kinase C가 배양한 계배근원세포의 분화에 미치는 영향 (Effects of $Ca^2+$ and Protein Kinase C on the Chick Myoblast Differentiation)

  • 정기화;김세재;박정원;박영철;이정주
    • 한국동물학회지
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    • 제32권1호
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    • pp.40-47
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    • 1989
  • 계배 근원세포의 배양 배지에 calcium ionophore A23187이나 EGTA를 배양 24시간에 첨가함으로서 초래된 세포내 칼슘 농도의 변화는 근원세포의 분화과정에 상당한 영향을 미쳤다. 배양 24시간에 A23187이나 EGTA를 첨가한 후 배양 48시간, 72시간, 및 96시간에 각각 세포를 [35S]methionine으로 1시간 표지시킨 후 수확하여 2차원 전기영동법으로 단백질을 분리시켰을 때, 일부 단백질은 배양 조건에 따라 합성 양상을 달리함을 보였다. 배양 24시간에 처리한 A23187과 calcium-activated neutral protease는 대조군에 비해 세포융합을 촉진시켰으나 동일 시기에 처리된 phosphoprotein을 정량함으로써 조사하였을 때, A23187이 배양 초기에는 대조군에 비해 약간 이 효소의 활성도를 높이는 효과를 보였으나 세포융합이 완성된 시기인 96시간에는 대조군에 비해 활성도를 높이는 효과를 보였으나 세포융합이 완성된 시기인 96시간에는 대조군에 비해 활성도의 차이를 나타내지 않았다. A23187 및 calcium-activated neutral protease에 의한 세포융합의 촉진, 그리고 A23187에 의한 protein kinase C 활성도의 증가가 모두 근원세포의 융합이 활발히 진행되는 시기인 배양 48-72 시간에 관찰됨을 볼 때, 세포내 칼슘의 농도는 protein kinase C 및 calcium-activated neutral protease와 상호연관을 가지면서 세포분화에 관여하는 것으로 사료된다.

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지방질원으로서 어유가 백서 상피세포의 인지질 조성 및 Protein Kinase C 활성에 미치는 영향 (Effect of Menhaden Oil Feeding on Protein Kinase C Activity and Membrane Phospholipid Profiles in Mouse Epidermal Cells)

  • 최면
    • Journal of Nutrition and Health
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    • 제27권5호
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    • pp.419-428
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    • 1994
  • To investigate the effect of dietary menhaden oil on protein kinase C (PKC) activity and membrane phospholipid composition in epidermal cells, female BALB/C mice were fed either menhaden oil or corn oil with two different levels(5% or 20%) for 6 weeks. Membrane phosphatidycholine(PC) was decreased in menhaden oil-fed group. Eicosapentaenoic acid(EPA) and Docosahexaenoic acid(DHA) were only presented in the acyl chain of membrane phospholipid of menhaden oil-fed mice, so that membrane fluidity of the group could be different from the other group. Both cytosolic and membrane-associated PKC activity in epidermal cells were decreased in menhaden oil-fed mice when compare with corn oil-fed mice. Furthermore, rate of PKC transfer from cytosol to membrane in menhaden oil-fed group was not as fast as in corn oil-fed group. Based on these observations, dietary menhaden oil might act differently from other dietary fat in carcinogenesis.

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수종 생약의 Protein kinase C 저해활성 (Inhibitory Activity against Protein Kinase C of Some Medicinal Plants)

  • 이현선;안순철;김보연;박문수;오원근;윤병대;안종석;민태익
    • 생약학회지
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    • 제23권3호
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    • pp.142-145
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    • 1992
  • MeOH extract of twenty medicinal herbs were screened for their effects against protein kinase C (PKC) using bleb-forming assay and PKC enzyme assay. Smilax china and Sanguisorba officinalis showed potent anti-PKC activity. Campsis grandiflora and Galla Halepensis showed moderate inhibitory effect on PKC.

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대두 유식물에서 Protein Kinase C의 부분 정제 (Partial Purification of Protein Kinase C in Glycine max)

  • 최윤희
    • Journal of Plant Biology
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    • 제36권2호
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    • pp.171-176
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    • 1993
  • Protein kinase C, a protein related in PI cascade, was partially purified from the cytosol protein of etiolated plants of Glycine max by DEAE-52 cellulose chromatography and phenylsepharose chromatography. When the DEAE column was eluted with 0-0.8 M linear gradient KCl, tow fractions were found that increased the phosphorylation of histon H1 about five and nine-fold in the presence of 5 $\mu\textrm{g}$/mL phosphatidylserine and 0.5 $\mu\textrm{g}$/mL diolein, respectively. These fractions were used as DEAE pool. The reaction eluted with relatively high concentration of KCl was loaded on phyenylsepharose column with 5 mM CaCl2 and eluted with 1 mM EGTA. A fraction contained the protein kinase C, which increased the phosphorylation of the histon H1 was fractionated. To determine the molecular weight of PKC, the fraction eluted from phenylsepharose column was analyzed by 5~15% polyacrylamide gel electrophoresis after concentrated with the Amicon membrane (YM10). That revealed two bands corresponding to 60 and 65 kGy by silver staining of the gel, respectively.

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Type II Cell 분리체로부터 Surfactant 인지질의 분비를 매개하는 신호변환 기전 (Signal Transduction Mechanisms Mediating Surfactant Phospholipid Secretion in Isolated Type II Cell)

  • 박성수
    • Tuberculosis and Respiratory Diseases
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    • 제43권2호
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    • pp.123-127
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    • 1996
  • Secretion of surfactant phospholipid can be stimulated by a variety of agonists acting via at least three different signal transduction mechanisms. These include the adenylate cyclase system with activation of cAMP-dependent protein kinase; activation of protein kinase C either directly or subsequent to activation of phosphoinositide-specific phospholipase C and generation of diacylglycerols and inositol trisphosphate; and a third mechanism that involves incresed $Ca^{2+}$ levels and a calmodulin-dependent step. ATP stimulates secretion via all three mechanisms. The protein kinase C pathway is also coupled to phopholipase D which, acting on relatively abundant cellular phospholipids, generates diacylglycerols that further activate protein kinase C. Sustained protein kinase C activation can maintain phosphatidylcholine secretion for a prolonged period of time. It is likely that interactions between the different signaling pathways have an important role in the overall physiological regulation of surfactant secretion.

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