• Journal of Microbiology and Biotechnology
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• v.18 no.8
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• pp.1453-1458
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• 2008

For quantitative PCR assay of Salmonella enterica serovar Typhimurium in food samples, a real-time PCR method was developed, based on DNA genome equivalent. Specific primers and probe designed based on the STM4497 gene of S. Typhimurium LT2 showed the specificity to S. Typhimurium. Threshold cycle (Ct) values of real-time PCR were obtained from a quantitative standard curve with genomic DNA of Salmonella Typhimurium. In addition, the recovery of S. Typhimurium inoculated artificially to chicken samples with $4.5{\times}10^5$ to 4.5 CFU/ml was evaluated by using real-time PCR and plate-count methods. Result showed that the number of cells calculated from the real-time PCR method had good correlation with that of the plate-count method. This real-time PCR method could be applicable to the detection and quantification of S. Typhimurium in food samples.

• Education of Primary School Mathematics
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• v.25 no.3
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• pp.233-249
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• 2022

Measurement plays an important role in both school mathematics and real life. Among the measurement areas, length is the first to learn and is the basis for measurement. Time is measured in its own way and is characterized by being the most abstract. This study attempted to analyze elementary mathematics textbooks and representations in real life to examine how the length and time of learning in school mathematics differ from those represented in real life. Based on this, we tried to derive implications for the direction of measurement education and elementary math textbooks. As a result of the analysis, the concept of length was used the same in real life and school mathematics. However, terms such as distance, depth, and height were not defined, and the representation of the approximate value was presented in a fragmentary form. In addition, there were parts where students were likely to feel confused in school mathematics and real life, such as the same units such as 'minutes and seconds' were used in time. Therefore, considering these differences, it is necessary to consider the direction of composition of math textbooks and teaching and learning so that students can connect school mathematics and real life and understand widely about measurement concepts.

• Communications of Mathematical Education
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• v.21 no.2 s.30
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• pp.177-197
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• 2007

This study analyzes the theoretical background concerning problem solving, mathematization and real-life problems. Further it examines how middle school mathematics teachers and high school students of first grade recognize the real-life problems provides in textbooks concerning the area of geometry. Following those results found from this analysis, this paper reveals the issues and problems that we noticed through the analysis of real-life problems from textbooks, level 8 and level 9, Also we suggest the application of them along with the development of real-life problems for students' uplifting problem solving skills.

• Proceedings of the Korea Society for Energy Engineering kosee Conference
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• 1999.05a
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• pp.171-176
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• 1999

Nondestructive inspection and accompanying life analysis based on fracture mechanics were the major conventional methods for evaluating remaining life of critical high temperature components in power plants. By using these conventional methods, it has been difficult to perform in-service inspection for life prediction. Also, quantitative damage evaluation due to unexpected abrupt changes in operating temperature was almost impossible. Thus, many efforts have been made for evaluating remaining life during operation of the plants and predicting real-time life usage values based on the shape of structures, operating history, and material properties. In this study, a core software for on-line life monitoring system which carries out real-time life evaluation of a critical component in power boiler(high temperature steam headers) is developed. The software is capable of evaluating creep and fatigue life usage from the real-time stress data calculated by using temperature/stress transfer Green functions derived for the specific headers and by counting transient cycles. The major benefits of the developed software lie in determining future operating schedule, inspection interval, and replacement plan by monitoring real-time life usage based on prior operating history.

• Fisheries and Aquatic Sciences
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• v.24 no.11
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• pp.351-359
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• 2021

The red sea bream iridovirus (RSIV) belonging to genus Megalocytivirus is responsible for red sea bream iridoviral disease (RSIVD) in marine and freshwater fishes. Although several diagnostic assays for RSIV have been developed, diagnostic sensitivity (DSe) and specificity (DSp) of real-time polymerase chain reaction (PCR) assays are not yet evaluated. In this study, we developed a TaqMan probe-based real-time PCR method and evaluated its DSe and DSp. To detect RSIV, the probe and primers were designed based on consensus sequences of the major capsid protein (MCP) genes from megalocytiviruses including RSIV, infectious spleen and kidney necrosis virus (ISKNV), and turbot reddish body iridovirus (TRBIV). The probe and primers were shown to be specific for RSIV, ISKNV, and TRBIV-types megalocytiviruses. A 95% limit of detection (LOD_95%) was determined to be 5.3 viral genome copies/µL of plasmid DNA containing the MCP gene from RSIV. The DSe and DSp of the developed real-time PCR assay for field samples (n = 112) were compared with those of conventional PCR assays and found to be 100% and 95.2%, respectively. The quantitative results for SYBR Green and TaqMan probe-based real-time PCR were not significantly different. The TaqMan probe-based real-time PCR assay for RSIV may be used as an appropriate diagnostic tool for qualitative and quantitative analysis.

• Food Science of Animal Resources
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• v.41 no.1
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• pp.85-94
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• 2021

A pig-specific real-time PCR assay based on the mitochondrial ND5 gene was developed to detect porcine material in food and other products. To optimize the performance of assay, seven commercial TaqMan master mixes and two real-time PCR platforms (Applied Biosystems StepOnePlus and Bio-rad CFX Connect) were used to evaluate the limit of detection (LOD) as well as the PCR efficiency and specificity. The LODs and PCR efficiencies for the seven master mixes on two platforms were 0.5-5 pg/reaction and 84.96%-108.80%, respectively. Additionally, non-specific amplifications of DNA from other animal samples (human, dog, cow, and chicken) were observed for four master mixes. These results imply that the sensitivity and specificity of a real-time PCR assay may vary depending on master mix and platform used. The best combination of master mix and real-time PCR platform can accurately detect 0.5 pg porcine DNA, with a PCR efficiency of 100.49%.

• Korean Journal of Child Studies
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• v.22 no.4
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• pp.149-166
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• 2001

This study examined differences in function, structure, and satisfaction of adolescent friendships by differing patterns of internet usage and of real life versus internet friendships. Subjects were 396 male and female 10th grade students. Data were collected with structured questionnaires and analyzed by SPSS. Findings were that the internet functioned as a major field of interpersonal relationships for Korean adolescents. Among 3 types of internet usage patterns, pursuit of interpersonal relationship, constituted most of the functioning and the most intimate friendships and game pleasure had the fewest and least intimate friendships both in real life and on the internet. Subjects addicted to the internet had the most functioning friendships and the highest level of intimacy in friendships. As the level of internet addiction increased, the level of satisfaction in friendships in real life decreased while the level of satisfaction of friendships through the internet increased.

• ETRI Journal
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• v.28 no.4
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• pp.537-540
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• 2006

When multiple ZigBee wireless personal area networks (WPANs) are in close proximity to each other, contentions and collisions in transmissions will lead to increased packet delays. However, there is no existing study on how delay performance would be affected in a crowded real-life environment where each person walking down a busy street would be wearing a ZigBee WPAN. This letter studies the use of ZigBee WPANs in such a real-life environment for real-time heart beat monitoring. To be pragmatic, we derived a mobility pattern from the analysis of a real-life video trace. Then, we estimated the delay performance from the video trace by combining data collected from ZigBee experiments. The results show that the 300 ms packet delay requirement will not be met for only 11% of the time. When failure occurs, it will last for an average duration of 1.4 s.

• Design Convergence Study
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• v.14 no.4
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• pp.151-167
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• 2015

This research suggests scenarios of learning in real life contexts with smart devices and provides user interface prototypes to show their characteristics more effectively. For this, literature review was conducted to understand the strength and limitations of current learning in which real life contexts are used. Then, the characteristics of smart devices as a support tool of learning service was reviewed. With such understand, a user research was executed to establish 10 design guidelines for learning service in real life contexts. Finally, this paper suggested two scenarios that shows learning in real life contexts as case studies and their detailed user interface protypes.

• Economic Analysis
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• v.26 no.1
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• pp.133-166
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• 2020

Since the mid-1990s, Korea has been aging rapidly. At the same time, real interest rates have declined sharply. This paper studies whether population aging has contributed to the real interest rate decrease in Korea. We first present empirical evidence that increases in life expectancy and the old-age-dependency ratio, and a fall in population growth, i.e., the population aging, decrease real interest rates. Then we calibrate a life-cycle model to capture the features of the old-age-dependency ratio and population growth in Korea, and show that population aging accounts for about one third of the fall in real interest rates between 1995 and 2018. Furthermore, according to simulation results, increased life expectancy is more important than decreased population growth in affecting the real interest rate decrease during the period.