• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.33-41
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• 2004

The type of trichomes, their density and pattern of distribution on leaves of 16 genotypes of mulberry, belonging to both diploid and polyploid categories, were studied by scanning electron microscope. The present investigation was undertaken to find out the relationship of physical attributes, especially the density and trichome types with higher acceptability and better rearing performance by the silkworm Bombyx-mori L. Two types of trichomes glandular and non-glandular types were observed on both the leaf surfaces of all the mulberry genotypes studied. In general, greater densities of trichomes were observed on the abaxial surface than the adaxial surface of leaves in most of the genotypes. Distribution of glandular trichomes were more in abaxial surface and non-glandular trichomes were more in adaxial surface. Overall, distribution of glandular and non-glandular trichomes per unit area of leaf did not follow any regular pattern. When leaves of those genotypes were fed to silkworms, trichome density was found to be significantly negatively correlated with the survival of larvae i.e., effective rate of rearing, but trichome density did not influence the economic characters of rearing. As the distribution of glandular trichomes (GT) and non-glandular trichomes (NGT) did not follow any definite pattern, no relation could be established between the GT and NGT densities with silkworm rearing performance. However, the ratio of GT and NGT in a particular genotype influenced the rearing parameters, higher the ratios better the rearing performance. High GT and NGT ratio (＞1.00) was found positively significant when correlated with economic parameters viz., larval weight, single cocoon weight and single shell weight. The study is useful in screening different mulberry genotypes for their better acceptability to silk-worm and higher rearing performance at the early stage of selection without actually conducting the rearing.

• 한국해양생명과학회지
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• 제7권2호
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• pp.213-220
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• 2022

본 연구는 저염분 상태에서 수온 및 사육밀도와 같은 환경요인이 흰다리새우(Litopenaeus vannamei)의 성장에 미치는 영향을 조사하기 위하여 수행하였다. 연구 결과, 흰다리새우는 저염분 상태에서 전반적으로 수온이 높을수록 생존율이 높게 나타났고, 사육밀도가 낮을수록 생존율이 높아졌다. 사료효율에 관한 연구에서는 수온이 높을수록 증체량(WG)이 증가하였고, 사육밀도가 증가할수록 증체량은 감소하였다. 수온이 높을수록(수온 31℃) 성장 속도가 빨랐다. 또한 사육밀도에 따른 성장도의 평가에서도 사육밀도가 낮은 상태에서 성장 속도가 빨라지는 것을 확인하였다. 본 연구 결과를 흰다리새우의 최적 성장을 위한 적정 사육밀도 및 사육수온의 결정에 유용하게 적용되리라 사료된다.

• 한국양식학회지
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• 제1권1호
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• pp.13-24
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• 1988

양식용 넙치 종묘의 효율적인 대량생산 방법에 관한 기초적 자료로서 치어의 사육밀도를 검토함과 동시에 고밀도사육의 가능성에 대하여 조사하고자, 폐진순환여과 사육수조를 전장2.53$\pm$0.24 cm, 체고 1.12$\pm$0.12 cm의 넙치치어를 저면적 137.75 $cm^2$당 10개체 (D10), 20개체 이용하여 (D2O), 30개체 (D30) 및 40개체 (D40) 식수용한 4개 밀도구를 설정한후 65일간 사육한 결과는 다음과 같다. 사육기간중의 수온은 $21.0\~27.0^{\circ}C$로 자연수온에 비해 높았고,비중(${\delta}_{15}$)은 1.024$\~$1.026으로 비교적 높은 편이었다. 사육기간중의 용존산소는 5.4$\~$7.5 ml/$\iota$ 범위였고, 무기태질소로서 $NH_4^+-N$은 0.07$\~$0.48 ppm, $NO_2^{-}-N$은 0.006$\~$0.33 ppm이었으며, 특히, $NO_3^{-}-N$은 3.89$\~$34.06 ppm으로 매우 높았다. 밀도별 치어의 전장성장은 D20에서 8.17$\pm$0.80 cm로 가장 빨랐고, D10에서 7.72$\pm$0.40 cm로 가장 느렸으나, 밀도구별 성장차이에 대한 유의의 차는 없었다. 밀도별 치어의 체고성장은 D20에서 4.l6$\pm$0.39 cm로 가장 빨랐고, D10에서 3.94$\pm$0.21 cm로 가장 느렸으나, 밀도구별 성장차이에 대한 유의의 차는 없었다. 밀도별 치어의 전장에 대한 체고의 상대성장비는 D10이 b=0.5346으로 가장 높았고, B30이 b=0.5165로 가장 낮았으나, 밀도구간의 유의의 차는 인정되지 않았다. 밀도구별 치어의 생존율은 D10, D20, D30 공히 $90\%$로 높았으나, D40은 $75\%$로 낮은 편이었다. 수용밀도의 지표로서, 안측 체표면적이 수조의 저면적을 덮는율을 구하기 위하여 전장과 체고를 곱한 면적 (X)과 안측 체표면적 (Y)의 상관관계는 Y=0.5994X+0.1840으로 표시되었다. 각 밀도구별 모든 개체의 안측 체표면적이 저면적을 덮는율은, 실험종료시 D10의 1.2배로부터 D4O의 4.1배까지로 나타나, 치어의 고밀도사육이 가능한 것으로 판단되었다.

• 한국해양생명과학회지
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• 제3권2호
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• pp.67-73
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• 2018

본 연구는 수온, 염분, 사육밀도 및 혼합 먹이생물 공급량과 같은 환경요인이 왕우럭 Tresus keenae의 유생사육 시 생존 및 발생에 미치는 영향을 조사하기 위하여 수행하였다. 연구 결과, 왕우럭 유생사육의 최적 조건은 수온 20℃에서 24℃, 염분 30 psu, 사육밀도 1 ml당 5개체 및 먹이생물 I. galbana, C. simplex 및 T. suecica 혼합 공급량 10×10⁴ 세포/ml이였다. 본 연구 결과를 통해 밝혀진 왕우럭 유생 최적의 사육 환경요인은 빠른 성장 및 높은 생존을 통해 생산성 향상에 기여하리라 생각된다.

• Fisheries and Aquatic Sciences
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• 제14권2호
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• pp.123-129
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• 2011

To determine whether rearing density affects the hypermelanosis on the blind side (ambicoloration) of olive flounders Paralichthys olivaceus, we reared fry with an unpigmented non-eyed (blind) side in duplicate at densities of 150 individuals/$m^2$ (commercial production density: control) and 450 individuals/$m^2$ (high density group) for 90 days in 1-t dark-green fiberglass reinforced plastics (FRP) tank. We recorded feed intake, feed conversion efficiency (FE), growth and survival, and measured the ratios of staining blind-side area (staining area) and ambicolored fish every 30 days. Daily feed intake (DFI), feed conversion efficiency (FE), growth rate, condition factors, and survival rate were calculated at the end of the experiment. Although the FE was higher in the high-density compared to the control, the two density groups showed similar feed intakes, growth, and survival. The ratio of staining area as well as the ratio of ambicolored fish significantly were significantly higher in the high-density group than in the control from days 30 to 60, but significantly increased and evened out by the end of the experiment (P<0.05). In conclusion, we determined that rearing density is not the main cause of the blind-side hypermelanosis, but found that increasing the rearing density can accelerate the ambicoloration in olive flounders.

• Journal of Forest and Environmental Science
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• 제34권1호
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• pp.61-65
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• 2018

To know if small changes in rearing water temperature and density affect the number of bacterial and fungal colonies in metamorphosed frogs, Dybowski's frog tadpoles were reared from Gosner 25-26 stages at either low ($1^{\circ}C$ low to ambient water temperature), ambient, or high ($1^{\circ}C$ high) water temperature (each 15 tadpoles in 20 L water) condition and at either low (10 tadpoles/20 L water), medium (20 tadpoles), or high (30 tadpoles) density condition. Immediately after metamorphosis, we sampled bacteria and fungi from skin, liver, and heart of six metamorphosed frogs, randomly selected for each treatment group. After separate incubation of bacteria and fungi on 3M Petrifilm plates, we counted the number of bacterial and fungal colonies appeared on the plates and compared the numbers among the temperature and density treatment groups. For temperature treatment, high-temperature group had fewer bacterial colonies, while low-temperature group had more fungal colonies than the other two groups. For density treatment, low-density group had fewer bacterial colonies than the other two groups, but the number of fungal colonies were not different among the groups. Our results suggest that small increased rearing water temperature and lowered rearing density could potentially reduce pathogens in farming frogs.

• 한국수산과학회지
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• 제44권3호
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• pp.251-258
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• 2011

To investigate whether the rearing environmental factors (size heterogeneity, rearing density, and feeding frequency) affect the growth and cannibalism of fry in cultured puffer Takifugu obscurus, we conducted three experiments. To examine size effects, we used small (total length [TL] $26.0{\pm}0.5\;mm$, body weight [BW] $0.18{\pm}0.01\;g$), medium (TL $23.1{\pm}0.1\;mm$, BW $0.28{\pm}0.01\;g$), large (TL $26.0{\pm}0.2\;mm$, BW $0.48{\pm}0.01\;g$) and small-large size combination groups. For density, we used size-matched puffers (TL $25.0{\pm}0.5\;mm$, BW $0.53{\pm}0.03\;g$) and five density were examined ranging from 1.43 to 7.14 individuals/L. For the feeding frequency, we also used size-matched fry and feeding frequencies of three times/2 days, one time/day, two times/day, three times/day. We ran each experiment in triplicate and investigated the survival rate, daily food intake (DFI), feed efficiency (FE), and daily growth rate (DGR). The growth of the puffers increased with increasing size, density, and feeding frequency, while cannibalism increased with a greater size gap, density and lower feeding frequency. Therefore, we concluded that size, rearing density, and feeding frequency are major factors influencing growth and cannibalism of the puffer, T. obscurus.

• 한국수산과학회지
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• 제44권4호
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• pp.359-365
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• 2011

This study was conducted to investigate the production, elver stocking, rearing facilities and rearing method of eel culture to determine aquaculture management conditions for optimal rearing of eel Anguilla japonica. The production of eel culture was evaluated by the proportion of eels from the main inland fin fish species production in Korea. Elver stocking was assessed by the elver stocking densities of pond and recirculation culture. Rearing facilities were investigated according to the rearing tank size proportion of the pond and recirculation culture. We selected sample farms by region and by size. We visited sample farms and recorded the number of elvers stock for pond area, size of tanks, feed and feed quantity, and the size and number of harvest eels. The production capacity of Jeollanam-do and Jeollabuk-do were 71.9% and 21.3% respectively. This production quantity represented 93.2% of the total Korean eel production quantity. In Jeollanam-do, there are 236 eel farms, 202 pond farms, and 34 recirculation aquaculture facilities. The elvers' first density data by each aquaculture method revealed that elvers' first density varied more in recirculation system farms, as compared to pond aquaculture. In intensive pond farms, the elvers' first density decreased as the size of farm increased. There was a correlation between the size of tank(x) and the facility of a water wheel for dissolved oxygen in pond culture systems(y=0.022x-0.494; $R^2$=0.860). Another strong correlation was found between the weight of eel(x) and eel density(y) in pond culture systems(y=283.5x-0.27; $R^2$=0.992). Finally, there was a strong correlation between the length of eel(x) and the weight of eel(y) in intensive pond culture(y=0.0005x-3.2783; $R^2$=0.9775). The final survival rate did not differ significantly among pond sizes and culture types.

• 환경생물
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• 제35권2호
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• pp.152-159
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• 2017

For the effective seedling production of the hard shelled mussel, Mytilus coruscus, this study assessed the effects of the dietary value of live food, density, water temperature and salinity on growth and survival rate of the larvae. The optimal survival rate and growth rate were examined under differing conditions of water temperature, salinity, and rearing density for 30 days. The three groups were provided different feeding organisms, such as Isochrysis galbana and Teleaulax suecica. The mixtures were provided at a rate of $5{\times}10^4cell\;mL^{-1}$. The best growth was observed in the group with conditions $21^{\circ}C$ water temperature ($16.2{\pm}9.1{\mu}m$), 33 psu of salinity ($16.82{\pm}3.9{\mu}m$), $2500individual\;m^{-2}$ ($17.2{\pm}5.9{\mu}m$), and fed with $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture ($16.0{\pm}7.3{\mu}m$). The highest survival rate was found in the group at conditions $18^{\circ}C$ water temperature (66.4%), 33 psu of salinity (24.4%), $2500individual\;m^{-2}$ (65.8%), and fed with $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture (58.8%). We therefore conclude that the suitable culture conditions for the stable production of hard shelled mussel artificial seedlings was at 18 to $21^{\circ}C$ of temperature, 30 to 33 psu of salinity, 2500 to $5000individual\;m^{-2}$ of rearing density, and feeding supplement of $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture under semi running water system.

• 한국축산시설환경학회지
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• 제17권sup호
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• pp.7-20
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• 2011

본 연구는 친환경 한우생산을 위해 다양한 원료사료의 선택 및 구성비율 변경을 통해 메탄 저감용 사료배합비의 작성 및 적정 사육밀도에 관한 연구를 규명하기 위하여 실시하였다. 본 연구의 메탄 저감용 사료배합에 활용한 원료사료는 파쇄 옥수수와 알팔파 외 21종이었다. 또한 적정 사육밀도를 조사하기 위한 공시동물은 평균 체중 150 kg의 한우 송아지 12두를 선정하여 4개월간 실험을 수행하였다. TMR 1, 2, 3에 비해 TMR 4에서 약 3~4배정도 낮은 메탄가스 발생량을 보였으며, 사료가격에서도 저렴한 결과를 보였다. TMR 4는 TMR 1, 2 및 3에 비해 건물 소실율에서 낮은 경향을 보였으며, 메탄생성량은 24~37%정도 감소하는 결과를 보였다. 한편, 한우 육성우의 적정 사육밀도 실험에서 저밀도 ($18m^2/head$) 및 일반 처리구 ($9m^2/head$)는 과밀도 처리구 ($6m^2/head$)에 비해 총 증체량, 일당증체량 및 사료요구율이 개선되는 결과를 보였다. 또한 건강과 질병에 관련된 혈액성분에서 저밀도 및 일반 처리구는 영양대사 및 건강에 관련된 total protein, glucose, AST, ALT 및 GGT 농도가 과밀도 처리구에 비해 효과적으로 개선되는 결과를 보였다. 체위 발육(development of body size)의 경우 저밀도 및 일반 처리구의 체중, 흉폭, 요각폭 및 곤폭은 고밀도 처리구에 비해 현저하게 증가하였으며, 특히, 저밀도 처리구는 일반 처리구보다 체위발육이 우수한 결과를 보였다. 그리고 저밀도 및 일반 처리구는 고밀도 처리구에 비해 깔짚의 수분 함량, 대장균 및 콕시듐의 조절에서도 긍정적인 결과를 보였다. 이와같이 한우 육성우의 적정 사육밀도는 두당 $6m^2$ 이상이 바람직한 것으로 판단된다. 따라서 본 연구에서는 원료사료의 선택 및 구성비율 변경을 통해 메탄 생성량을 저감시킬 수 있는 가능성을 사료자원의 배합비 조정으로 메탄 저감용 사료생산의 가능성을 제시하였으며, 사육밀도는 한우의 성장과 발육에 중요한 요인임을 알 수 있었다. 따라서 본 연구에서는 적절한 원료사료의 선택 및 구성비율 변경을 통해 메탄 발생량 조절의 가능성을 제시하였으며, 사육밀도는 한우의 성장과 발육에 중요한 요인임을 확인할 수 있었다.