• Bulletin of the Korean Chemical Society
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• v.14 no.4
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• pp.510-514
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• 1993

The retention behavior of proteins was investigated by using reversed-phase chromatography (RPC), comparing to the retention behavior of small molecules in RPC. The evaluation was carried out on a SynChropak RP-P($C_{18}$) column with 0.1% aq. TFA-organic solvent modifier such as acetonitrile, isopropanol, and ethanol. The Z value (the number of solvent molecules required to displace the solute from the surface) was a general index for the characterization of protein retention as a function of organic concentration over a range of temperature between 5 and 70$^{\circ}C$. Van't Hoff plots provided the basis for evaluating the enthalpic and entropic changes associated with the interaction between protein and the stationary phase. Z values did not change significantly at the range of temperature showing the consistent ${\Delta}H^{\circ}$ and ${\Delta}S^{\circ}$ values. From these investigation, it was concluded that the retention behavior of proteins in RPC was able to be predicted by the retention parameters applied to small molecules. Furthermore, myoglobin and hemoglobin in RPC as stated above showed a similar retention behavior regardless of their molecular weights.

• YAKHAK HOEJI
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• v.53 no.5
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• pp.281-285
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• 2009

The retention of solutes in reversed-phase high-performance liquid chromatography depends on their hydrophobicity. Although the retention behaviors of alkyl benzenes have been reported so far, quite a few authors have mentioned the retention behavior of alkyl benzenes with plural hydrophobicity parameters. In this sense, we were interested in the retention behaviors of alkyl benzenes having benzene moiety and increasing alkyl chain. In this study, we therefore investigated the retention behavior of alkyl benzenes in reversed-phase high-performance liquid chromatography in order to obtain information concerning the effects of the aromatic moiety and the carbon chain on the retention mechanism by comparing their capacity factor (k') in relation to the carbon chain length. The eluent acetonitrile ($CH_3CN$) showed high selectivity on alkyl benzenes, showing the high difference of capacity factor (${\Delta}log\;k'$) between toluene and octyl benzene. Indeed, the ${\Delta}log\;k'$ of 80% $CH_3CN$ represented 1.42- and 4.25-times longer than 90% MeOH and 60% THF, respectively. The hydrophobicity parameters, van der Waals volume, bond constant, partition constant, $\pi$-energy effect and enthalpy were evaluated with the capacity factor (k') of alkyl benzenes eluted on 80% CH3CN, 90% MeOH and 60% THF, respectively. The best eluent for predicting retention behavior of alkyl benzenes was 90% MeOH ($R^2$ 0.999). The three parameters, van der Waals volume, bond constant and partition constant were well coincident to log k' by increasing alkyl benzenes. However, $\pi$-energy effect and enthalpy were severely disagreeable. Taken together, van der Waals volume, bond constant and partition constant were a reliable parameters to predict the retention behaviors of alkyl benzenes on reversed-phase column.

• Bulletin of the Korean Chemical Society
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• v.14 no.4
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• pp.515-519
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• 1993

The retention behavior of proteins was investigated by using hydrophobic interaction chromatography (HIC), comparing to the results obtained in reversed-phase chromatography (RPC) described in the previous paper. A SynChropak propyl column was employed with 0.05 M phosphate buffer (pH 7.0) containing sodium sulfate. Conformational changes were recognized by examining Z values as a function of sodium sulfate concentration over a range of temperature between 5 and 65$^{\circ}C$. Z values did not change significantly at the range of the temperature showing the consistent ${\Delta}H^{\circ}$ and ${\Delta}S^{\circ}$ values. The sign and the magnitude of ${\Delta}H^{\circ}$ and ${\Delta}S^{\circ}$ of proteins in HIC were compared with those obtained in RPC. The signs of ${\Delta}H^{\circ}$ and ${\Delta}S^{\circ}$ of proteins in HIC were all positive, while those of proteins in RPC were all negative. These results suggested that the retention of proteins in HIC and in RPC were entropy-driven and enthalpy-driven process, respectively. From the two different investigations, it was concluded that the retention mechanism of RPC and HIC was based on the same fundamental principle in which separation is dependent on hydrophobicity, but the retention behavior of the proteins in HIC is clearly different from that observed in RPC.

• The Journal of Engineering Geology
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• v.19 no.4
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• pp.475-482
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• 2009

The behavior of earth retention wall installed in cut slope is different from the behavior of retention wall applied in urban excavation. In order to establish the design method of anchored retention walls in cut slope, the behavior of anchored retention wall can be investigated and checked in detail. In this study, the behavior of anchored retention wall was investigated by instrumentation installed in cut slope for an apartment construction stabilized by a row of piles. The horizontal displacement of anchored retention wall was larger than the displacement of slope soil behind the wall at the early stage of excavation. As the excavation depth became deeper, the horizontal displacement of slope soil was larger than the displacement of anchored retention wall. It means that the horizontal displacement of anchored retention wall due to excavation is restrained by soldier pile stiffness and jacking force of anchor. Jacking force of anchor was mainly influenced in the horizontal displacement of anchored retention wall. The displacements of anchored retention wall and slope soil were affected mainly by an rainfall infiltrated from the ground surface. Meanwhile, the horizontal displacement of anchored retention wall with slope backside was about 2-6 times larger than the displacement of anchored retention wall with horizontal backside of excavation.

• The Journal of Engineering Geology
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• v.15 no.2 s.42
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• pp.155-168
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• 2005

The behavior of earth retention wall installed in a cut slope is different from the behavior of retention wall applied in an urban excavation. In order to establish the design method of anchored retention wall in the cut slope, the behavior of anchored retention wall needs to be investigated and checked in detail. In this study, the behavior of anchored retention wall was investigated by the instrumentation installed in the cut slope, where was stabilized by a row of piles in an apartment construction site. The horizontal displacement of anchored retention wall was larger than the displacement of slope soil behind the wall at the early stage of excavation. As the excavation depth became deeper, the horizontal displacement of slope soil was larger than the displacement of anchored retention wall. It means that the horizontal displacement of anchored retention wall due to excavation is restrained by soldier pile stiffness and jacking force of anchor at the early stage of excavation. lacking force of anchor was mainly influenced on the horizontal displacement of anchored retention wall. The displacements of anchored retention wall and slope soil were affected mainly by rainfall infiltrated from the ground surface. Meanwhile, the horizontal displacement of anchored retention wall with a sloped backside was about $2\~6$ times larger than the displacement of anchored retention wall with a horizontal backside of excavation.

• Journal of the Korean Chemical Society
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• v.34 no.1
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• pp.44-50
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• 1990

The liquid chromatographic retention behavior of structural isomers of phenols was investigated by a change of the mobile phase properties. The retention behavior of structural isomer of phenols in reversed phase liquid chromatography was affected by eluent electrolyte added. It can be seen that this behavior is illustrated by a mechanism of Langmuir isotherm and ion exchange between phenolate and the reversed phase coated with ions. The retention behavior was represented as two different areas according to the concentration of the electrolytes. These areas can be explained as counter ion and co-ion effect, respectively. The maximum retention values were dependent not upon the kinds of organic modifier but upon the kinds of electrolyte.

• Geomechanics and Engineering
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• v.30 no.4
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• pp.373-382
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• 2022

Unsaturated soil at high suctions is widespread. Many civil engineering projects are related to the hydro-mechanical behavior of unsaturated soils at high suctions, particularly in arid and semiarid areas. To investigate water retention behaviors of nine clayey soils (one is classified as fat clay and the others are classified as lean clay according to the unified soil classification system), the high suction (3.29-286.7 MPa) was imposed on the specimens at zero net stress by the vapor equilibrium technique. In this paper, the effect of void ratio on the water retention behavior at high suction was discussed in detail. Validation data showed that soil types, i.e., different mineralogical compositions, are critical in the soil water retention behavior at a high suction range. Second, the hysteresis behavior at a high suction range is mainly related to the clay content and the specific surface area. And the mechanism of water retention and hysteresis behavior at high suctions was discussed. Moreover, the maximum suction is not a unique value, and it is crucial to determine the maximum suction value accurately, especially for the shear strength prediction at high suctions. If the soil consists of hydrophilic minerals such as montmorillonite and illite, the maximum suction will be lower than 10⁶ kPa. Finally, using the area of hysteresis to quantify the degree of hysteresis at a high suction range is proposed. There was a good correlation between the area of hydraulic hysteresis and the specific surface area.

• Bulletin of the Korean Chemical Society
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• v.13 no.3
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• pp.240-245
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• 1992

The effect of the addition of sodium dodecyl sulfate (SDS) to a buffered mobile phase (pH 3.4) on the retention of nucleotides, nucleosides and bases was investigated with a polyvinyl alcohol (PVA) column. Depending on the concentration of SDS, two different trends in the retention of nucleosides and bases containing an $NH_2$ group were observed. If the concentration of SDS was less than 5.5 mM, the retention of compounds containing an $NH_2$ group increased as the concentration of SDS in the mobile phase increased. In contrast, if the concentration was greater than 5.5 mM, the retention of compounds containing an $NH_2$ group decreased. Thus, the SDS acted as an ion-pairing reagent at lower concentration but formed micelles at higher concentrations. The retention behavior of the nucleosides and bases in the presence of a micellar concentration of SDS in the mobile phase on the PVA column was compared to the retention behavior on other types of columns.

• Journal of the Korea Society of Computer and Information
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• v.18 no.10
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• pp.225-234
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• 2013

The purpose of this study is to evaluate the impact of airline's retention equity on customer positive exclusive behavior intention and to minimize customer churn based maintenance is aimed to derive key variables in air transport market. A survey was conducted Incheon and Gimpo airport to use in the national carrier of domestic air travelers. A total of 480 respondents completed a survey. The result reveal that loyalty program, preferential treatment & acknowledgement program, and community program have significantly effect on positive behavior intention. However, preferential treatment & acknowledgement program, and community program have significantly effect on exclusive behavior intention. It showed that they are more influence than loyalty program as a switching barrier of airlines. The academic and practical implication of this study has been identified in the competitive market to maximize customer retention factors of maintaining retention equity to derive empirical strategic priorities.

• Journal of the Korean Geotechnical Society
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• v.20 no.9
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• pp.57-64
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• 2004

In order to establish the design method of anchored retention walls in cut slope, the behavior of anchored retention walls and backside ground needs to be investigated and checked in detail. In this study, the behavior of anchored retention walls was investigated by instrumentation installed in cut slope for an apartment construction site stabilized by a row of piles and anchored retention walls. When the anchor was installed at each excavating stages, the horizontal deflection of retention wall decreased, while the horizontal deformation of backside ground increased. The deflection of anchored retention wall decreased as the anchor was prestressed. The prestressed anchor farce has a great effect on the deflection of retention walls, while it has little effect on the deformation of its backside ground. The maximum horizontal deflection of anchored retention walls was developed between $1\%\;and\;4\%$ of excavation depth, which are $2\~8$ times larger than max. horizontal deflection of anchored retention walls including rock layers with backside horizontal ground. Meanwhile, SLOPILE (ver. 3.0) program analyzes the slope stability effects for anchored retention walls. As a result of analysis on slope stability analysis, the lateral earth pressure applied at anchored retention piles could be used as the mean values of empirical lateral pressures using anchored retention wall with horizontal ground at its backside.