• Journal of Biosystems Engineering
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• v.27 no.5
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• pp.381-390
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• 2002

Direct seeding of rice-seed pellets is expected to be an alternative for solving problems in current direct seeding cultivation of rice. but mass production of rice-seed pellets is prerequisite for practical application. Design. construction and performance evaluation of an experimental rice seed pelleting machine were carried out for mass production of rice-seed pellets. The pelleting machine intended to make a ball type rice-seed pellet, which have 3∼5 rice seeds and diameter of which is 12 mm. Pellet materials ; rice seeds, soil, and binder were mixed and kneaded by the mixer. The designed rice seed pelleting machine fed pellet materials by screw conveyor to forming rolls and made rice-seed pellets. Capacity, ratio of perfect rice-seed pellets, seed and pellet material loss were investigated as mixing ratio of soil to rice seed and feeding rate of pellet materials. The pelleting machine showed up to 37,000 pellets/h of pelleting rate, 61∼71% of weight ratio of perfect rice-seed pellets to pellet materials supplied, 17∼48% of seed loss ratio. Average weight and average diameter of the pellets were 1.66 g and 12.0 mm. respectively. More than 3 rice seeds were included in most pellets at 6 : 1 of mixing ratio of soil to rice seed. And compression strength of the pellets was in the range of 88-130 N. To improve performance of the pelleting machine, improvements of the forming rolls, feeding mechanism, and discharging mechanism for reducing loss of pellet materials and seeds damage are needed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.1
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• pp.53-58
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• 2006

This study was conducted to investigate the crossability, seed dormancy and overwintering ability of rice plant in GM (glufosinate ammonium-resistant lines. Iksan 483 and Milyang 204) and non-GM (their parents) or red rice (Andongaengmi). Seed-setting rate was not significantly different between GM and non-GM rice varieties. Iksan 483 and Milyang 204 showed the similar level of seed germination rate from 30 to 50 days after heading as compared to non-GM rice varieties. After overwintering in paddy field, seed germination rate of GM and non-GM rice varieties ranged from 14.3 % to 57.6 % in dry soil condition, but there was no germination in wet-soil except red rice. The result in wet-soil condition may help to set up a strategy for reducing the risk of gene flow of transgene via dispersal of seeds of GM plants. The crossability, seed dormancy and seed overwintering of Iksan 483 and Milyang 204, herbicide resistant GM rice varieties, were not significantly different compared to non-GM rice varieties. The results might be helpful to reduce the risk of transgene dispersal from GM crop via seeds and pollens.

• Journal of Biosystems Engineering
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• v.26 no.2
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• pp.115-122
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• 2001

An impact-type seed-metering device was developed for the planting of rice-pellets. In this study, new design of pellet-metering device focused on simplicity and precision seeding for the planting of rice-seed pellets. In addition of seed-metering device, several devices were also developed such as seed-guiding device, seed-supplying tube and furrow opener for precision pellet planting. Field test was conducted to estimate the planting performance of the developed metering device. As a cam rotates, the impact bar of the metering device pushes a rice-seed pellet so that the seed can be discharged from the seed-supplying tube in the impact-type seed-metering device. Results of the tests showed that mean seeding spacing was 12cm at the traveling speed of 1.0m/s, corresponding to a target spacing for planting of rice-seed pellets. Also, both miss-seeded rate and damaged-seed rate were less than 2.0%, indicating acceptable levels for the precision planting. The developed mechanism of the impact-type metering device can be directly applied to the design of metering devices for the precision pellet planting of other crops.

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.157-157
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• 2023

Pre-harvest sprouting (PHS) in rice (Oryza sativa L.) is one of the main problems associated with seed dormancy. PHS causes yield loss and reduction of grain quality under unpredictable humid conditions at the ripening stage, thus affecting the economic value of the rice crop. To resolve this issue, it is important to understand the molecular mechanism underlying seed dormancy in rice. Recent studies have shown that seed dormancy is affected by a large number of genes associated with plant hormones. However, the effect of heat stress on seed dormancy and plant hormones is not well understood. In this study, we compared the PHS rate as well as the transcriptome and small RNAome of the seed embryo and endosperm of two different accessions of rice, PHS-susceptible rice (low dormancy) and PHS-resistant rice (high dormancy) under three different maturation stages. We identified and verified the candidate genes associated with seed dormancy and heat stress-related responses in rice using quantitative real-time PCR. We newly discovered hormone-related genes, heat shock protein-related genes, and miRNAs potentially involved in PHS. These findings provide a foundation for understanding the dynamics of transcriptome and small RNAome of hormone- and heat stress-related genes, which affect PHS during seed maturation.

• Journal of Crop Science and Biotechnology
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• v.11 no.3
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• pp.193-198
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• 2008

To develop a natural re-seeding technology, the optimum soil incorporation time of Chinese milk vetch(CMV) plant residues, seed persistence in soil, biomass production and subsequent rice yield were determined from 2005 to 2007 in rice with CMV green manure in southern Korea. Generally, insufficient seedling stand was regenerated with CMV incorporation to the soil at 20 and 25 days after flowering(DAF), while high regeneration of greater than 400 seedlings per $m^2$ was observed at 35 and 40 DAF. High re-seeding stand of CMV incorporated at 35 DAF or later was related with high seed viability and heavy seed weight. Appreciable number of CMV seeds remained 4 month after burial in soil and a good number of CMV seedlings regenerated from this seed bank at rice harvest time in the fall. Based on the relationships among re-seeding number of CMV plants, seed weight and seed viability, CMV plants should be incorporated into the soil 35 DAF(May 30) or later when CMV seeds were sufficiently matured. The natural re-seeding stand for the 3-year trials was stable ranging from 437 to 700 plants per $m^2$ and the biomass production was sufficient to supply nitrogen for rice growth. The use of re-seeding CMV plant can produce similar rice yield like that of rice without CMV green manure.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.31-31
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• 2017

Understanding the mechanism(s) to overcome or prevent seed ageing deterioration during storage is of fundamental interest to seed physiologists. Vitamin E (tocols) is known as a key metabolite to efficiently scavenge lipid peroxy radicals which cause membrane breakdown resulting in seed ageing. However, in rice research this hypothesis has been tested for very few lines only without considering intraspecific variation in genomic structure. Here, we present a correlation study between tocols and seed longevity using a diverse rice panel. Seeds of 20 rice accessions held in the International Rice Genebank at the International Rice Research Institute, representing aus, indica, temperate japonica and tropical japonica subpopulations, were used for tocols analysis (quantification of ${\alpha}$-, ${\beta}$-, ${\gamma}$-, ${\delta}$-tocopherol/tocotrienol by ultra performance liquid chromatography) and storage experiments at $45^{\circ}C$ and 10.9% seed moisture content (sample taken for germination testing every 3 days up to 60 days). To examine interactions between DNA sequences and phenotype, the 700k high-density single-nucleotide polymorphism marker data-set was utilized. Both seed longevity (time for viability to fall to 50%; $p_{50}$) and tocols content varied across subpopulations due to heterogeneity in the genetic architecture. Among eight types of tocol homologues, ${\alpha}$-tocopherol and ${\gamma}$-tocotrienol were significantly correlated with $p_{50}$ (negatively and positively, respectively). While temperate japonica varieties were most abundant in ${\alpha}$-tocopherol, indica varieties recorded 1.3 to 1.7-fold higher ${\gamma}$-tocotrienol than those of other subpopulations. It was highlighted that specific ratio of tocol homologues rather than total tocols content plays an important role in the seed longevity mechanism.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.439-447
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• 2010

Riptortus clavatus, one of the many insects in major crops, damages pods and seeds, which reduces seed vigor and viability in soybeans. This study was conducted to examine the effect of diversely damaged seeds by R. clavatus on seed germination and seedling emergence and to determine the association of damaged seed with quality and yield of soybean sprouts. All seeds damaged by R. clavatus significantly (P<0.05) reduced seed vigor as measured by the rates of seed germination, germination speed, and seedling emergence. Mean seed germination rate of non-damaged seeds in sprout-soybean varieties was 97.8%, whereas the rates of seeds damaged at different levels, 31-50% and 51-80%, were 23.0 and 5.4%, respectively. The rates of seedling rot and abnormal, incomplete germination significantly (P<0.05) increased as the amount of seeds damaged by R. clavatus increased to 5, 10 and 15% against the total seeds for sprout production. Yield of soybean sprouts from seeds damaged at different levels decreased up to 13% as compared to that in normal seeds. In customer preferences on soybean sprout produce, 84% of customers participated in survey preferred to purchase sprouts from seeds with 5% of damaged seeds, but sprouts produced from seeds with 15% of damaged seeds were intended to purchase only by 22% of the customers. Areas of the seed damaged by R. clavatus were readily infected by pathogens as the seed germinated, resulted in deteriorated quality and reduced yield of sprout produce.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.4
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• pp.421-426
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• 2008

Freshly harvested seed of Chinese milk vetch (Astragalus sinicus L.; CMV) was strongly dormant because of hardseedness. Seeds of freshly harvested germinated only 8% while clipping the seed coat completely overcome the innate dormancy, which indicates inhibition of germination of the seed is mainly due to seed coat (87%). The dormant (intact) hard seeds did not imbibe water whereas the non-dormant (clipped) seeds took up rapidly. In natural environment condition, the hard seed coat dormancy was broken only after 5 months after seed harvest. To break such a strong seed coat dormancy, the chemical and heat treatments were effective. Concentrated sulfuric acid was more effective than dry heat and hot water treatments. Hot water treatment improved germination but the germination percentage was less than 41%. Treatments increased germination due to its effect on the seed coat integrity. A scanning electron microscope reveled that disruption of seed coat layers and subsequent development of numerous crack in the hilum region of the seed and on the seed coat surface of concentrated sulfuric acid treatment and formation of cracks in the dry heat treatments, respectively, were observed in the seed coat surface, which served as water entry points.

• Journal of Biosystems Engineering
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• v.28 no.5
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• pp.403-410
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• 2003

To enhance the performance of a rice seed pelleting machine and the quality of rice-seed pellets made, improvement of the rice seed pelleting machine developed previously(Park, 2002) was tried and its performance was evaluated. As compared with the previous pelleting machine, a feeding mechanism of pellet materials to the forming rolls was changed from screw conveyor to hydraulic cylinder for proper feeding, rings were installed among rows of semi-spherical forming grooves on the forming rolls for reducing pellet materials loss and seeds damage, and discharging air nozzles were added for complete discharging of the pellets made. Through performance tests, capacity, pelleting ratio, and seed loss ratio of the pelleting machine were investigated at the mixing ratios of soil to rice seed of 6 : 1, 7 : 1, and 8 : 1, and rotating speeds of the forming rolls of 7 rpm, 10 rpm, and 13 rpm. As results of performance evaluation, pelleting ratios were in the range of 77 ∼ 89 ％, and maximum pelleting ratio increased by 18 % in comparison with that of the previous machine. Maximum capacity was about 110 kg/h(about 63,000 pellets/h), which was increased by 70 % in comparison with that of the previous machine. But, ratios of seed loss were in the range of 24 - 49 ％, which were not improved.

• Korean Journal Plant Pathology
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• v.1 no.1
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• pp.51-55
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• 1985

This study was carried out to observe the propagule of Fusarium moniliforme on the surface of rice seed and in the vascular bundle of rice stem by scanning electron microscope. Spore and mycelium of F. moniliforme were observed on the surface of rice seed and in the vascular bundle of rice stem. After seed treatment with Benlate T and Busan 3D, F. moniliforme was not isolated from chaffs, but frequently from brown rice, irrespective of disinfection period.