• Title/Summary/Keyword: rockfish

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Rockfish (Sebastes schlegeli) Vitellogenin: Purification, Characterization and Development of Sandwich ELISA System

  • Jung Jee Hyun;Kim Dae Jung;Han Chang Hee
    • Fisheries and Aquatic Sciences
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    • v.7 no.3
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    • pp.99-108
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    • 2004
  • Vitellogenin (VTG) was purified from serum of $estradiol-l7{\beta}-treated rockfish$(Sebastes schlegeli) by precipitation with $EDTA-Mg^{2+}$ and ammonium sulfate and two step chromatography (anion exchange chromatography and gel permeation chromatography) was performed on FPLC system. Rockfish VTG (rfVTG) was characterized and its properties were determined. The monomers have apparent, molecular mass of about 188 kDa as indicated by SDS-PAGE. Amino acid composition analysis of rfVTG was similar to VTG from other oviparous teleosts. Cysteine and lysine were present at relatively high level. Leucine was present at relatively lower level than in other species. The N-terminal amino acid sequence was evaluated to identify rfVTG. Western blot analysis using an antibody against the purified VTG showed that the antibody reacted with both plasma of $estradiol-l7{\beta}-treated rockfish$ treated male and purified VTG, whereas there was no reaction with male serum of the control. An ELISA was developed using monoclonal and polyclonal antibodies against rfVTG. The assay range was 3.2 ng/mL and 1,000 ng/mL and the value of the intra and inter assay variations were within $9.7{\%}$ and $11.2{\%}$, respectively. Recovery rate was $96.8{\%}$. The sandwich ELISA could be useful for the detection of VTG and could be good for screening of estrogenic compounds.

Kinetic of Copper Accumulation and Elimination in Rockfish (Sebastes schlegeli) Tissues Exposed to Dietary Copper

  • Kim Seong-Gil;Jee Jung-Hoon;Kim Sang-Gyu;Kang Ju-Chan
    • Fisheries and Aquatic Sciences
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    • v.6 no.4
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    • pp.213-219
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    • 2003
  • Experiments were carried out to investigate the accumulation and elimination changes in the tissue of juvenile rockfish (Sebastes schlegeli) after sub-chronic dietary Cu (0, 50, 125, 250 and 500 mg/kg) exposure for 60 days and depuration for 30 days. The profile of Cu accumulation in the tissue of rockfish was dependent on the exposure periods and Cu concentration. Liver of rockfish is a more important storage tissue than other tissues, and the order of Cu accumulation in tissues was liver > intestine > kidney > gill > muscle. The accumulation factors were increased with the exposure period in gill, intestine, liver, kidney and muscle. An inverse relationship was observed between the accumulation factor and the exposure concentrations in the gill, kidney and muscle. Cu elimination in tissues of rockfish were decreased with periods for the 30 days of depuration except kidney and muscle. The order of Cu elimination in organs during depuration was intestine > liver > gill.

Method for Marking on Scales of Juvenile Black Rockfish by Immersion in Three Chemicals (세 화학 물질, Oxytetracycline hydrochloride, Alizarin red S 및 Calcein의 침지 처리에 의한 조피볼락 치어의 비늘 표지)

  • 노충환;최희정;박용주;홍경표;박철원;명정구
    • Journal of Aquaculture
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    • v.12 no.3
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    • pp.237-245
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    • 1999
  • In this study, we evaluated the efficiency of chemical marking of black rockfish scales by immersion in oxytetracycline hydrochloride (OTC, 500 ppm), alizarin red S (AR, 250 ppm) and calcein (CARL, 250 ppm) diluted rearing water. Immersion treatment of chemicals had no effects on both mortality and growth of black rockfish. Marking sucess was 100% in all treatment durations (24, 48 and 72 hours) with three chemicals and marking quality was higher in 48 and 72 hours than 24 hours treatment. Marking retention rates at 24 weeks after treatment were 100% in OTC and CAL treated group, but marking quality was higher in CAL treated group (brilliant 92%, bright 8% and dim 0%) than in OTC treated group (brilliant 4%, bright 70% and dim 26%). AR treated group had lower marking retention rates and marking quality than OTC and CAL treated group. As a results, immersion treatment with OTC and CAL was effective in marking scales of black rockfish and practical in releasing program and other studies requires same rearing environment.

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Changes on Hematological Factors and Oxygen Consumption of Korean Rockfish Sebastes schlegeli in High Water Temperature (고수온에서 조피볼락(Sebastes schlegeli)의 산소소비 및 혈액성상 변화)

  • DO, Yong-Hyun;MIN, Byung-Hwa;KIM, Young-Dae;PARK, Mi Seon
    • Journal of Fisheries and Marine Sciences Education
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    • v.28 no.3
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    • pp.738-745
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    • 2016
  • Aquaculture production of Korean rockfish Sebastes schlegeli has been continuously increased from 2000s and the fish has become the second most important mariculture fish in Korea. However, there are some environmental problems in aquaculture of Korean rockfish recently. In this regards, stress responses to high water temperature was examined via oxygen consumption, blood physiological parameter and endocrinological method. Oxygen consumption of Korean rockfish had significantly increased with rising water temperature. And oxygen consumption during the light time was no different with the dark time. The levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) showed no difference until $27^{\circ}C$, but it had rapidly increased at $30^{\circ}C$. Moreover high water temperature affected to increase in plasma glucose and cortisol levels.

Evaluation of Dietary Essentality of Ten Vitamins for Juvenile Black Rockfish (Sebastes schlegeli)

  • Lee, Hae-Young;Cho, Kee-Chae
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.261-262
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    • 2000
  • Juvenile black rockfish, Sebastes schlegeli is an important commercial species along the coast of Republic of Korea. The black rockfish possesse some desirable characteristics for aquaculture including tolerance to cold water and handling, and receptivity to induced spawning in captivity. (omitted)

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Correlation between Pharmacokinetics of Praziquantel and Extermination of Microcotyle sebastis (Monogenea) in Cultured Rockfish Sebastes schlegeli

  • Kim Chun Soo;Kim Ki Hong
    • Fisheries and Aquatic Sciences
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    • v.4 no.4
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    • pp.197-200
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    • 2001
  • To investigate the re-treatment time of Microcotyle sebastis by oral administration of praziquantel, the residue levels of praziquantel in plasma of rockfish Sebastes schlegeli administered orally at a dose of 200 mg/kg B.W. were analyzed by reversed-phase HPLC, and the concentrations of praziquantel in the plasma were correlated with the extermination of M. sebastis. The absorption and depletion of praziquantel in the blood of rockfish were fast and the residual concentrations of praziquantel declined below $4\mu g/mL$ within 24 hr post treatment. Most of worms were exterminated within 3 hr after oral administration of praziquantel, however, a small number of M. sebastis were not killed by the treatment until end of the experiment. Considering fast drop of praziquantel in blood and extermination pattern of M. sebastis in the present results, retreatment at an interval of 9-12 hr would be effective for eradication of M. sebastis.

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Influence of Originated Host and Water Temperature on the Infectivity and Growth of Microcotyle sebastis (Monogenea)

  • Kim Ki Hong;Cho Jae Bum;Park Soo-Il
    • Fisheries and Aquatic Sciences
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    • v.1 no.1
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    • pp.79-81
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    • 1998
  • To investigate the influence of originated host and water temperature on the infectivity and growth of Microcotyle sebastis, juveniles of Schlegel's black rockfish (Sebastes schlegeli) were exposed to the eggs of M. sebastis originated from. Schlegel's black rockfish and black rockfish (Sebastes inermis) at $10^{\circ}C$ and $10^{\circ}C$. There were significant differences between the two isolates in the infectivity of eggs, and the growth of worms. The growth of M. sebastis was significantly affected by water temperature, and worms grew faster in $20^{\circ}C$ than in $10^{\circ}C$.

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Low-value Fish used as Feed is a Source of Disease in Farmed Fish

  • Kim, Do-Hyung
    • Fisheries and Aquatic Sciences
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    • v.18 no.2
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    • pp.203-209
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    • 2015
  • Low-value fish is the most commonly used feed in Asian fish farms despite the fact that its application is controversial in regard to the sustainability and biosecurity of aquaculture. In this study, the causal agent of a disease outbreak at a Korean rockfish Sebastes schlegeli farm was investigated to determine whether the low-value fish used at the farm was the source. Infected Korean rockfish and Pacific sand eel used as feed were sampled from the farm, and bacterial cultures recovered from the internal organs of all sampled rockfish were isolated as pure cultures and later identified as Vibrio harveyi. The causal agent of the disease was also isolated from the kidneys of some of the sampled Pacific sand eels. This study provides additional evidence that the low-value fish used as feed at fish farms can be a key source of infectious diseases.

Seasonality and the microhabitat of Microcotyle sebastis Goto, 1894, a monogenean gill parasite of farmed rockfish, Sebastes schlegeli Hilgendorf, 1889 (조피볼락의 아가미에 기생하는 단생흡충 Microcotyle sebastis Goto, 1894의 계절별 감염 및 기생 특성)

  • 윤길하;엔드류쉰;크리스티나좀머빌;조재윤
    • Journal of Aquaculture
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    • v.10 no.4
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    • pp.387-394
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    • 1997
  • A total of 353 farmed rockfish, Sebastes schlegli Hilgendorf, 1880 were sampled from 3 localities on Korean coastline over a 12 month period. Full parasitological examination revealed the polypisthocotylean monogenean Micorcotyle sebastis Goto, 1894 to be abundant with infections consistently reaching over 90~100% prevalence throughout the year. A seasonal pattern in parasite abundance on the gills is evident, with the population peaking twice, the largest in winter and again in summer. While the parasitic load on the left and right gills was not dissimilar, parasites within the gill sets were found to favour settlement of the II and III gill arches. A comparison of parasite abundance with host length, revealed that the smaller length fish classes (<17cm standard body length) had significantly heavier infections than those of larger fish (>17 cm standard body length). This report represents the fist record of Microcotyle sebastis on farmed rockfish in Korean coastal waters.

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Effect of formalin killed vaccine of atypical Aeromonas salmonicida for black rockfish (Sebastes schlegeli) (조피볼락(Sebastes schlegeli)에 대한 비정형 Aeromonas salmonicida 포르말린 사균 백신의 효과)

  • Kim, Wi-Sik;Lee, Hyeon-Ho;Oh, Myung-Joo;Han, Hyun-Ja
    • Journal of fish pathology
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    • v.31 no.1
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    • pp.35-39
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    • 2018
  • Atypical furnculosis caused by atypical Aeromonas salmonicida, is an emerging problem of farming of rockfish (Sebastes schlegeli) in Korea. In this study, protection against atypical furunculosis was compared in rockfish vaccinated with A. salmonicida formalin killed cell (FKC) and adjuvant containing FKC. The formalin inactivated A. salmonicida vaccine provided a low protection of 20% and 10% relative percent survival (RPS) at 44 and 58 days post vaccination. However, addition of adjuvant (squalene and aluminum hydroxide) into inactivated A. salmonicida vaccine clearly enhanced the level of protection showing 70% and 50% RPS at 44 and 58 days post vaccination.