• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.528-532
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• 2004

We have studied physiological responses of mung bean sprout to the treatment of elicitors. Chemicals such as salicylic acid and methyl jasmonic acid are not only the intermediates found in plant defense system but also could affect plant secondary metabolism. We found that mild treatment of salicylic acid and acetyl salicylic acid (aspirin) increase isoflavone production dramatically in mung bean sprout which has very low level of isoflavones compared with soybean sprout. The isoflavone content in salicylic acid treated- and acetyl salicylic acid treated-mung bean sprout was about 2.3 and 2.2 times higher than that of control, respectively. However, the increasing patterns of isoflavone in cotyledon and hypocotyl and root were not identical. The major increase among isoflavone fractions in cotyledon was led by the increase in malonylglycitin and malonyldaidzin level. Whereas, the increase in hypocotyl and root was led by malonyldaidzin. Methyl jasmonic acid did not show statistically significant increase in mung bean sprout. With this result, we were able to propose the non-transgenic method, which can control the isoflavone production in germinating mung bean.

• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.598-602
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• 1998

Twenty two rhizobacteria were isolated from the roots and rhizosphere of radish, carnation, potato and tomato. There isolates produced a fluorescent pigment in King's B medium and identified as Pseudomonas spp. These isolates colonized roots and rhizosphere of the host plants. In the study of cultural characteristics of the bacteria, the pH of the culture broth was changed from neutral (7.0) to alkali (8.8∼9.41) and the numbers of cells were increased from 106 to 108 after 40 hr of incubation in basal standard succinate medium. The salicylic acid production identified by pink color reaction were observed in 7 bacteria. Out of these 7 salicylic acid producing bacteria, only 2 strains of bacteria such as Pseudomonas fluorescens RS006, and Pseudomonas sp. EN401 were confirmed as salicylic acid producers by optical density measurement. Therefore, for screening of salicylic acid producing bacteria from the roots and rhizosphere, color reaction of the culture medium should be done in the first step, and then optical density measurement of culture extract should be made for the confirmation of salicylic acid production.

• Journal of Microbiology and Biotechnology
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• v.9 no.4
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• pp.482-487
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• 1999

The Pseudomonas sp. strain NGK1 (NCIM 5120) was immobilized in calcium alginate, agar, and polyacrylamide gel matrices. The salicylic acid-producing capacity of freely suspended cells was compared with immobilized cells in batches with a shake culture and continuous culture system in a packed bed reactor. Freely suspended cells ($4\times10^{10}cfu/ml$) produced 12 mM of salicylic acid, whereas cells immobilized in calcium alginate ($1.8\times10^{11}$cfu/g beads), agar ($1.8\times10^{11}$cfu/g beads), and polyacrylamide ($1.6\times10^{11}$cfu/g beads) produced 15, 11, and 16mM of salicylic acid, respectively, from naphthalene at an initial concentration of 25 mM. The continuous production of salicylic acid from naphthalene was investigated in a continuous packed bed reactor with two different cell populations. The longevity of the salicylic acid-producing activity of the immobilized cells from naphthalene was also studied in semi continuous fermentations. The immobilized cells could be reused 18, 13, and more than 20 times without losing salicylic acid-producing activity in calcium alginate-,agar-, and polyacrylamide-entrapped cells, respectively. The study reveals a more efficient utilization of naphthalene and salicylic acid production by the immobilized Pseudomonas sp. strain NGK1 as compared to the free cells.

• Journal of Korean Society of Forest Science
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• v.100 no.2
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• pp.178-183
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• 2011

This study was carried out to investigate the dose-dependent effect of salicylic acid on both the adventitious root growth and the accumulation of various eleutherosides in the bioreactor culture of Eleutherococcus senticosus. The highest biomass production (5.4 g DW/L) was observed in the absence of salicylic acid, while the root growth was significantly decreased by increasing the concentration of salicylic acid. Salicylic acid stimulated the production of both eleutheroside B, E and $E_1$. The highest levels of eleutheroside B ($179.5{\mu}g/g$ DW), E ($1169.9{\mu}g/g$ DW) and $E_1$ ($45.4{\mu}g/g$ DW) were obtained by the addition of $80{\mu}M$ of salicylic acid. The maximum eleutheroside production was $4975.8{\mu}g/L$ when salicylic acid was not added. In addition, when the adventitious roots were cultured in the basal medium supplemented with $80{\mu}M$ of salicylic acid, the highest levels of eleutheroside B was observed at the 9th day, while eleutheroside E and $E_1$ were observed at the 6th day, respectively.

• Journal of Pharmaceutical Investigation
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• v.12 no.4
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• pp.126-131
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• 1982

A quantitative fluorometric method was developed to determine aspirin and salicylic acid in bulk aspirin and commercial aspirin tablets. The excitation maximum for aspirin was observed at 280 nm and the emission maximum was at 335nm. The lowest energy excitation band for salicylic acid was at 308nm and the fluorescence emission band was at 450nm. Excipients, binders, lubricants and impurities did not interfere. Excellent recoveries were obtained for aspirin and salicylic acid. Results obtained by the KP III procedure and the proposed method were compared.

• Journal of Convergence for Information Technology
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• v.12 no.5
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• pp.298-304
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• 2022

In this study, it is intended to provide basic research data on hair loss and problematic scalp that is expanding to lower age groups and women. When using Company B's hair ampoule containing L-Menthol, Salicylic acid, and Dexpanthenol daily for women in their 20s with problematic scalp, the effect on the scalp and hair after 4 weeks was investigated. As a result of this study, when using Company B's ampoule containing L-Menthol, Salicylic acid, and Dexpanthenol, the subjects showed improvement effects such as relieving inflammation, reducing scalp fever and erythema, reducing itching and hair loss, and increasing hair thickness. In addition, it was confirmed that the scalp self-diagnosis of the subjects showed improvement effects on scalp satisfaction, scalp vitality and elasticity, heat generation, scalp pulling after cleaning, and itching. Through these research results, it is believed that products containing L-Menthol, Salicylic acid, and Dexpanthenol will help improve problematic scalp and hair.

• Archives of Pharmacal Research
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• v.8 no.3
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• pp.99-107
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• 1985

The interaction of reduced riboflavin 2', 3', 4', 5'-tetrabutyrate with salicylic acid, aspirin, and salicylamide has been spectroscopically investigated to determine the binding mechanism. Hydrogen-1 and carbon-13 unclear magnetic resonance, infrared, and absoption spectra were measured in chloform-d and chloroform. The association of the reduced riboflavin with salicylic acid derivatives is different from that osidizd one. Salicylic acid and the reduced riboflavin form a cyclic hydrogen bounded complex through the imino (3-N, 5-N) protons and the carbonyl (2-C, 4-C) oxygens of the isolloxazine ring of the latter, and the carboxylic hydroxyl proton and carbonyl oxygen of the former. Aspirin and the reduced riboflavin form a complex by the same mode as salicylic acid. Salicylamide forms a cyclic hydrogen bonded complex with the reduced riboflavin through the imino (3-N, 5-N) protons and the carbonyl (2-C, 4-C) oxygens of the isoalloxazine ring, and the amino proton and the carbonyl oxygen of salic aylmide. It appears that both the oxidized and reduced form of riboflavin are associated with salicylic acid derivatives.

• Applied Chemistry
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• v.15 no.1
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• pp.29-32
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• 2011

Surfactant enhanced fluorescence (FL) of europium-salicylic acid (SA) complex has been studied. It was observed that weak FL of Eu(III) at the wavelength of 589 nm and 612 nm was found to be enhanced after addition of salicylic acid and cetyltrimethylammonium bromide (CTAB) surfactant upon excitation at 395 nm. Under optimized condition, FL intensity of Eu(III) at 612 nm responded linearly with the concentration of SA in the range of 5.5×10^-9 to 1.5×10^-6 M of SA. The detection limit was calculated from the calibration curve(3Sb/m) as 9.27×10^-9 M.

• Journal of Korean Society of Forest Science
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• v.85 no.3
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• pp.372-380
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• 1996

This study was carried out to study the resistant substances to pine needle gall midge and seasonal variation of phenolic compounds in pine needles among susceptible and resistant species to pine needle gall midge. Free and water soluble phenolic compounds contained in pine needles showed seasonal variations among pine species. But, catechol, vanillic acid and syringic acid including phenolic compounds showed no variation between pine species, even the amount of those component varied by the seasons. Salicylic acid in pine needles showed 140ppm in Pinus virginiana, 35ppm in Pinus thunbergii and 72ppm in hybrid pine, Pinus thunbergii${\times}$P. virginiana, but traces in Pinus densiflora on May and June, hatching season in larvae of pine needle gall midge. There was high level of salicylic acid in resistant species to pine needle gall midge but traces in susceptible species. As our previous researches, the necrosis rate of larvae of pine needle gall midge showed high level on the solution of phenolic compounds extracted from needles of P. virginiana and salicylic acid, and there was no gall formation in P. virginiana, so that salicylic acid in pine needles seems to have relation with those results. Therefore, it is estimated that salicylic acid is resistant substance to pine needle gall midge.

• Journal of the Korean Wood Science and Technology
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• v.26 no.3
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• pp.33-40
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• 1998

The phenolic acids in needles of five pine species such as Pinus densiflora, P. thunbergii. P. virginiana. P. rigida. and P. koraiensis were analyzed seasionally (March, June, September and December) in order to investigate the resistant factors against pine gall-midge(Thecodiplosis japonensis Uchida et Inouye). The interrelation of resistance to pine gall-midge and the content of phenolic acids in pine needles was investigated in the artificial hybride pine species. The contents of salicylic acid in susceptible species (P. densiflora and P. thunbergii) to pine gall-midge which watered with salicylic acid solution in a pot was determined. The results can be concluded as follows, 1. There was a little change in total phenolic constituents of resistant and susceptible pines seasonally. The each content of benzoic acid, salicylic acid and p-hydroxybenzoic acid in June was the highest through four seasons. 2. In June, resistant species showed higher content of salicylic acid than susceptible species, while the content of gallic acid was the highest in December. 3. Among the 21 artificial hybrids(P. densiflora ${\times}$ P. virginiana. P. thunbergii ${\times}$ P. virginiana), the hybrides of the higher salicylic acid content showed the lower rate of pine gall formation. 4. Pine gall formation of the susceptible species which were watered with salicylic acid solution remarkably decreased.