• Title/Summary/Keyword: salivary bacteria

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Two Sjogren syndrome-associated oral bacteria, Prevotella melaninogenica and Rothia mucilaginosa, induce the upregulation of major histocompatibility complex class I and hypoxia-associated cell death, respectively, in human salivary gland cells

  • Lee, Jaewon;Jeon, Sumin;Choi, Youngnim
    • International Journal of Oral Biology
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    • v.46 no.4
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    • pp.190-199
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    • 2021
  • Despite evidence that bacteria-sensing Toll-like receptors (TLRs) are activated in salivary gland tissues of Sjogren syndrome (SS) patients, the role of oral bacteria in SS etiopathogenesis is unclear. We previously reported that two SS-associated oral bacteria, Prevotella melaninogenica (Pm) and Rothia mucilagenosa (Rm), oppositely regulate the expression of major histocompatibility complex class I (MHC I) in human salivary gland (HSG) cells. Here, we elucidated the mechanisms underlying the differential regulation of MHC I expression by these bacteria. The ability of Pm and Rm to activate TLR2, TLR4, and TLR9 was examined using TLR reporter cells. HSG cells were stimulated by the TLR ligands, Pm, and Rm. The levels of MHC I expression, bacterial invasion, and viability of HSG cells were examined by flow cytometry. The hypoxic status of HSG cells was examined using Hypoxia Green. HSG cells upregulated MHC I expression in response to TLR2, TLR4, and TLR9 activation. Both Pm and Rm activated TLR2 and TLR9 but not TLR4. Rm-induced downregulation of MHC I strongly correlated with bacterial invasion and cell death. Rm-induced cell death was not rescued by inhibitors of the diverse cell death pathways but was associated with hypoxia. In conclusion, Pm upregulated MHC I likely through TLR2 and TLR9 activation, while Rm-induced hypoxia-associated cell death and the downregulation of MHC I, despite its ability to activate TLR2 and TLR9. These findings may provide new insight into how oral dysbiosis can contribute to salivary gland tissue damage in SS.

Antibacterial Effect of Electrolyzed Water on Oral Bacteria

  • Lee Sung-Hoon;Choi Bong-Kyu
    • Journal of Microbiology
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    • v.44 no.4
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    • pp.417-422
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    • 2006
  • This study investigated the antibacterial effect of electrolyzed water on oral bacteria both in vitro and in vivo. Tap water was electrolyzed in a water vessel using platinum cell technology. The electrolyzed tap water (called Puri-water) was put in contact with five major periodontopathogens or toothbrushes contaminated with these bacteria for 30 sec. In addition, Puri-water was used as a mouthwash for 30 sec in 16 subjects and the antibacterial effect on salivary bacteria was evaluated. Puri-water significantly reduced the growth of all periodontopathogens in culture and on toothbrushes, and that of aerobic and anaerobic bacteria in saliva, when compared to the effect of tap water. It also significantly reduced mutans streptococci growing on mitis salivarius-bacitracin agar. Our results demonstrate that the electrolyzed tap water is effective as a mouthwash and for toothbrush disinfection.

A Longitudinal Study on Salivary Microorgnisms and Immunoglobulin A after Delivery of Fixed Orthodontic Appliances (고정식 교정 장치물 장착후 타액내 미생물과 면역 글로블린 A의 변화)

  • 이현경;이광호;이승우
    • Journal of Oral Medicine and Pain
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    • v.23 no.2
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    • pp.109-117
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    • 1998
  • Fixed orthodontic appliances for the treatment of malocclusion has iatrogenic side effect such as demineralization of enamel, gingivitix and gingival hyperplasia. The purpose of this study is to longitudinally investigate the salivary microorganisms and immunoglobulin A after delivery of fixed orthodontic appliances for 10 months. Eight orthodontic patients were included in this study and the author has investigated the numbers of general bacteria, Streptococcus mutans Staphylococcus aureus and concentration of immunoglobulin A from unstimulated whole saliva. The author examined these parameters at prebracketing, 1 month after, 4 months after, 7 months after and 10 months after delivery of fixed orthodontic appliances. The obtained results were as follows : There were significant increases in the number of salivary general bacteria, Streptococcus mutans and Staphylococcus aureus after delivery of fixed orthodontic appliances The numbers of general bacteria were significantly increased at 1 month after (p<005), 4 months after (p<0.05), 7 months after (p<0.01), compared with prebracketing. However it showed no difference at 10 month after compared with 7 months after bracketing. The Numbers of Staphylococcus aureus were significantly increased at 1 month after (p<0.05), 4 months after(p<0.01), 7 month(p<0.01), compared with prebracketing. However it showed decreasing pattern at 10 months after compared with 7 months after bracketing. There was no significant difference in the concentration of immunoglobulin A after delivery of fixed orthodontic appliances.

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Changes of Oral Environment Between Probiotics and a Mouthwash Containing Essential Oil (구강 프로바이오틱스와 에센셜 오일 함유 양치액의 구강환경변화 )

  • Su-Young Lee
    • Journal of The Korean Society of Integrative Medicine
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    • v.11 no.3
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    • pp.219-226
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    • 2023
  • Purpose : The purpose of this study was to compare changes of salivary flow rate, salivary buffering capacity, and S. mutans after using a mouthwash containing essential oil or probiotics widely used as oral lactic acid bacteria in the oral environment. Methods : Fifty-three healthy adults aged 20 years or older voluntarily participated in this study after they were informed of the purpose of this study. Subjects were divided into a group treated with probiotics (L. reuteri) and a group treated with mouthwash containing essential oil. To evaluate changes in the oral environment, salivary flow rate, salivary buffering capacity, and S. mutans test were performed at baseline and 4 weeks later. Unstimulated and stimulated salivary flow samples were collected for 5 minutes and results were recorded in ml per minute. Salivary buffering capacity was divided into scores of 1 (low), 2 (moderate) and 3 (high) according to the color of the strip. The amount of S. mutans was classified as scores of 0, 1, 2, and 3 according to the decision table. Data collected in this study were analyzed at the 95 % significance level using the SPSS Version 23.0 program. Results : Both the unstimulated salivary flow rate and the stimulated salivary flow rate increased statistically after the intervention compared to those before intervention in the probiotics group. However, there was no statistically significant difference between the probiotics group and the mouthwash group. Salivary buffering capacity and S. mutans results showed no statistically significant differences between the probiotics group and the mouthwash group either. The salivary buffering capacity and the salivary flow rate showed a positive correlation. Conclusion : To improve the oral environment, such as salivary flow rate and salivary buffering capacity, an optimal product can be selected in consideration of individual preference and cost-effectiveness.

The Change of Salivary and Oral Bacteria Amount by Composition of SLS Contents of Toothpaste (세치제내 SLS함유에 따른 타액과 세균변화)

  • Shim, Youn-Su;Jeong, Mi-Ae;Jeong, Sang-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.9
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    • pp.3341-3346
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    • 2010
  • Dentifrices comtaining SLS of 2.2% and 0.0% were applied to 60 subjects for 4 weeks in this study. Dentifrice is used for salivary and oral bacteria amount change according to its SLS contents in toothpaste. The results were as follows. Anaysis of LSL contents befor and after the after experiment in salivary and bacterial count oral cavity didn't show significant difference in time interval. There was no correlation between salivary flow rate and bacterial count in the oral cavity according to content of SLS. Dentifrice of 2.2% content satisfied the subjects much more than that of 0.0% content(p<0.001).

Risk factors for the development of oral bacteria in workers according to oral environment (근로자의 구강환경요인에 따른 구강세균 발생의 위험요인)

  • Hong, Min-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.6
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    • pp.537-545
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    • 2016
  • This research examined the oral environmental factors to identify the risk factors for oral bacteria detection. This study comprised of 60 office workers aged between 20 and 65 years, and was performed from January 15 to February 28, 2015. The study variables measured were the stimulated and unstimulated salivary flow rates, salivary buffering, saliva pH, dry mouth at the dorsum of the tongue and the sublingual region, halitosis, and the degree of tongue-coating as oral environmental factors. To identify the presence of oral bacteria, pathogens were detected by extracting the gDNA of the resting salivary flow rate. The risk of S.mutans detection was 15 times higher with smokers, 1.3~1.6 times higher when the resting or stimulated salivary flow rate was reduced by 1 mm. The risk of P.intermedia detection was 13 times higher in smokers, 4.3 times higher as the severity of oral dryness was lowered, and 4 times higher for adults with a tongue coating than those without. In addition, the risk of detecting TM7 was 5.5 times higher as sublingual dryness was decreased by 1mm. The oral bacterial count will be reduced considerably by smoking cessation education and habits that facilitate a salivary flow rate. Furthermore, adults with good and well-managed dental hygiene are anticipated to have less oral bacteria and fewer dental diseases.

Evaluation of the Reliability of Salivary Testing Instrument (타액검사기의 신뢰도 평가)

  • Han, Keumah;Lee, Joonhaeng
    • Journal of the korean academy of Pediatric Dentistry
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    • v.45 no.3
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    • pp.363-369
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    • 2018
  • A salivary testing instrument has an advantage that the method is simple and can be performed in a short time. However, it is necessary to verify the factors that affect the reliability of the result, because the device is easy to use and even saliva collection is simple. The aim of this study was to compare the difference of the test results according to the measurement time in order to analyze the time factor of the external variable among the factors that may affect the measurement results of the salivary testing instrument. The relationship between the measured values of the salivary testing instrument to identify the internal variables was analyzed. Saliva was collected from 20 randomly selected patients regardless of age, sex, or diseases. The mean age was 46.6 years, 10 males and 10 females. The saliva collected was directly measured with the salivary testing instrument as group I. The saliva samples were placed in air in a paper cup for 10 minutes, and then measured as group III. Then group I was remeasured after 30 minutes and assigned as group II. Group III was remeasured after 30 minutes and called as group IV. As a result, all of the cariogenic bacteria, acidity, buffer capacity, blood, leukocyte, protein and ammonia, except buffer capacity, showed statistically significant changes in group II and IV. This means that the reliability of the test results is poor if the measurement time is not observed. Cariogenic bacteria were correlated with leukocyte and protein, buffer capacity was related to acidity, protein, and protein was related to buffer capacity and leukocyte. In conclusion, the result according to the measurement time as the external variable was different, which means that time must be strictly monitored when testing saliva. It is also necessary to take into account the relevance of the correlations between the internal variables and the clinical data.

The Effect of S. thermophilus Isolated from Saliva Treated with Phytoncide on P. gingivalis (피톤치드 처리 후 구강 내 잔존 S. thermophilus의 P. gingivalis에 대한 효과)

  • Jung, Sung-Hee;Auh, Q-Schick;Chun, Yang-Hyun;Hong, Jung-Pyo
    • Journal of Oral Medicine and Pain
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    • v.34 no.1
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    • pp.23-37
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    • 2009
  • The antibacterial effect of phytoncide on Porphyromonas gingivalis, which is the main causative agent of periodontal disease and halitosis, has been reported. However, little is known about its effect on normal oral microflora. The present study was performed to observe the effect of phytoncide on oral normal microflora and the inhibitory effect of surviving resident oral bacteria on P. gingivalis. In this study, saliva from each of 20 healthy subjects was treated with 1% phytoncide from Japanese Hinoki (Chamaecyparis obtusa Sieb. et Zucc.). Surviving salivary bacteria were isolated on blood agar plates and identified by 16S rDNA sequencing. In order to select inhibitory isolates against P. gingivalis, the isolates from the phytoncide-treated saliva were cultured with P. gingivalis. The results were as follows: 1. In general, the number of bacteria in saliva from periodontally healthy subjects was decreased when the saliva was treated with 1% phytoncide. 2. The majority of the salivary bacteria surviving the treatment of phytoncide were S. thermophilus (53%). 3. Most of the surviving salivary bacteria (72.5%) inhibit the growth of P. gingivalis A7A1-28 and P. gingivalis W83 on blood agar plates. 4. Among the surviving S. thermophilus, 85.8% of them were observed to inhibit P. gingivalis strains and 75.8% of the surviving S. sanguinis were inhibitory. Taken together, oral resident bacteria surviving phytoncide, which has been shown to inhibit P. gingivalis, may exert an additional inhibitory activity against the periodontopathic bacterium. Therefore, phytoncide can be used for preventing and ceasing the progress of periodontal disease and halitosis, and thus is expect to promote oral health.

Xylitol stimulates saliva secretion via muscarinic receptor signaling pathway

  • Park, Eunjoo;Na, Hee Sam;Jeong, Sunghee;Chung, Jin
    • International Journal of Oral Biology
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    • v.44 no.2
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    • pp.62-70
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    • 2019
  • Xylitol is well-known to have an anti-caries effect by inhibiting the replication of cariogenic bacteria. In addition, xylitol enhances saliva secretion. However, the precise molecular mechanism of xylitol on saliva secretion is yet to be elucidated. Thus, in this study, we aimed to investigate the stimulatory effect of xylitol on saliva secretion and to further evaluate the involvement of xylitol in muscarinic type 3 receptor (M3R) signaling. For determining these effects, we measured the saliva flow rate following xylitol treatment in healthy individuals and patients with dry mouth. We further tested the effects of xylitol on M3R signaling in human salivary gland (HSG) cells using real-time quantitative reverse-transcriptase polymerase chain reaction, immunoblotting, and immunostaining. Xylitol candy significantly increased the salivary flow rate and intracellular calcium release in HSG cells via the M3R signaling pathway. In addition, the expressions of M3R and aquaporin 5 were induced by xylitol treatment. Lastly, we investigated the distribution of M3R and aquaporin 5 in HSG cells. Xylitol was found to activate M3R, thereby inducing increases in $Ca^{2+}$ concentration. Stimulation of the muscarinic receptor induced by xylitol activated the internalization of M3R and subsequent trafficking of aquaporin 5. Taken together, these findings suggest a molecular mechanism for secretory effects of xylitol on salivary epithelial cells.

Studies on the Reduction of Nitrate and Formation of N-Dimethylnitrosamine by Streptococcus uberis in Human Saliva (Streptococcus uberis에 의한 질산염의 환원및 Dimethylnitrosamine의 생성에 관한 연구)

  • 정규찬;김종협;남경수
    • YAKHAK HOEJI
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    • v.30 no.1
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    • pp.8-13
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    • 1986
  • It has been assumed that nitrite, one of the precursor of N-nitrosamine, in human saliva must have been formed from salivary nitrate through the action of microorganism in the oral cavity. In this paper, we have tested the concentration of nitrite and nitrate in human saliva and the degrees of nitrate reduction by oral microflora and identified some bacteria which were able to reduce nitrate. The concentration of nitrite and nitrate was 1.7~9.5ppm and 9.0~28.5ppm respectively. The numbers of total bacteria and nitrate reducing bacteria in four korean human saliva sample were 15~63${\times}10^8$ CFU and 1.0~6.0${\times}10^8$ CFU and the main nitrate reducing bacteria were Streptococcus uberis which was presented in large quantities and showed remarkable reductive activity. Lastly, we knowed that N-dimethylnitrosamine was formed by the reaction between dimethylamine and nitrite in the presence of St. uberis in vitro.

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