• Korean Journal of Microbiology
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• v.37 no.1
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• pp.72-79
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• 2001

The effect of phenol on the change of bacterial community in the effluent water from a wastewater treatment plant was analyzed by PCR and terminal restriction fragment length polymorphism (T-RFLP). The fragments of 16S rDNA were amplified by PCR with bacterial primers, where one of the primers was biotinylated at the 5'-end. After digestion with restriction enzymes, HaeIII and AluI, the biotinylated terminal restriction tragments (T-RFs) of the digested products were selectively isolated by using streptavidin paramagnetic particles. The single-stranded DNA of T-RFs was separated by electrophoresis on a polyacrylamide gel and detected by silver staining technique. When 10 standard strains were analyzed by our method, each strain had a unique T-RF which corresponded to the calculated size from the known sequences of RDP database. The T-RFLP fingerprint generated from the effluent water was very complex, and the predominant T-RFs corresponded to members of the genus Acinetobacter, Bacillus and Pseudomonas. In addition, the perturbation of bacterial community was observed when phenol was added to the sample at the final concentration of 250 $l^{-1}$. The number of T-RFs increased and the major bacterial population could be assigned to the genus Acinetobacter, Comamonas, Cytophaga and Pseudomonas. A intense band assigned to the putative genera of Acinetobacter and Cytophaga was eluted, amplified, and sequenced. The nucleotide sequence of the T-RF showed close relationship with the sequence of Acinetobacter junii.

• Analytical Science and Technology
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• v.14 no.3
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• pp.274-285
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• 2001

In this study, the thermal desorption-cryofocusing-gas chromatographic(TD-C-GC) method was developed for determination of volatile organic compounds(VOCs) in ambient air and was applied at the municipal solid waste landfill sites. On-column cryofocusing was possible only with a 100 ml dewars bottle in TD-C-GC method with a stainless steel column. However, high operating pressure was needed for purging VOCs from the absorbent trap, which was able to solve by pressure programming with a electric pressure controller. By using both pressure and temperature programming brought increasing of resolution power in on-column cryofocusing method, but the high pressure caused a leakage of sample tube with repeated use. A loop cryofocusing devise was also developed and compared with the direct on-column method. In loop cryofocusing method, VOCs were concentrated on a 0.8mm i.d. loop which is located between the injector and separation column by using liquid nitrogen. In order to purge VOCs from the absorbent trap, only 0.4 psi of pressure was need in the loop cryofocusing method. Dual detection system was applied for the analysis of VOCs; a FID was used for hydrocarbons and a FPD was used for sulfur-containing compounds. Qualitative analysis was done by on-column cryofocusing GC-MS system. Among the large number of VOCs, toluene was the most abundant. Hydrogen sulfide, dimethyl sulfide, carbon disulfide, dimethyl disulfide and methyl propyl disulfide were detected at landfill site by FPD.

• Journal of the Society of Disaster Information
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• v.7 no.2
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• pp.151-160
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• 2011

This study aims to suggest the method for an effective conflict management to private security workers by investigating the impact of complicit management that recognized by private security, guard on trust for leader and turnover intention according to the complicit management type of private security, guard. The subjects was sampled by using purposive sampling method after selecting security, guard who work in private guard company registered in the Police Department in Seoul in 2010. Respondents of this study was sampled a total of 250 people and collected the sample of 234 people, and the number of cases used in the final analysis was 210 people. The entered data was processed by using SPSSWIN 17.0 Version to apply factor analysis, reliability analysis, correlation analysis, and multiple regression analysis. The result of reliability analysis of the questionnaire was Cronbach's ${\alpha}$ value of 0.838~0.859. First, combination, compromise, and grace the sub factors of the conflict management of private security, guard affect on trust for leader. Second, compromise, grace, and rule the sub factors of the conflict management of private security, guard has a negative effect on turnover intention. Third, the trust to leader has a negative effect on turnover intention.

• Korean Journal of Environmental Biology
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• v.23 no.3 s.59
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• pp.228-237
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• 2005

To compare the occurrence of Coleoptera by different sampling methods such as light trap, pitfall trap and sweeping, we collected samples every month from April to September,2004 in the Mt. Yeonyeop, Gangwon-do, Korea. According to the sampling methods, the species composition, abundance and dry weight were completely different. We collected 151 species in 35 families (690 individuals) by sweeping method, 148 species in 30 families (689 individuals) by light trap, and 112 species in 18 families (1,674 individuals) by pitfall trap, respectively. The dry weight in collected sample was about 181.46 g in pitfall trap,39.85 g in light trap, and 10.89 g in sweeping method, respectively. Relatively high flight and small-sized beetles such as Coccinellidae, Nitidulidae, Scarabaeidae were collected in light trap. The species diversity was high in July. Unlike the samples collected in light trap, the pitfall trap samples were big-sized saprophagous or carnivorous beetles such as Carabidae, Silphidae, Staphylinidae. The pitfall trap showed relatively the higher number of individual and lower species diversity compared to other methods. The major samples collected by sweeping method were small-sized carnivorous or herbivorous beetles such as Chrysomelidae, Curculionidae, Coccinellidae. The peak of species diversity occurred in May. The similarity was calculated with the Jaccard's index over the light trap-pitfall trap was 0.07, light trap-sweeping was 0.10, and pitfall trap-sweeping was 0.01. Consequently, similarity of sampling methods was relatively low. In conclusion, efficiency of the each sampling methods significantly differed in the species composition of Coleoptera. This study emphasize the necessity of using three sampling methods in the area of diversity research.

• Journal of the Korean Society of Food Culture
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• v.5 no.3
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• pp.331-347
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• 1990

This study was designed under the purpose to analyze the Korean Traditional Folk Remedies on food, to submit the basic statistical data, and to utilize them by examining how much they know about the Korean Traditional Folk Remedies and also by examining how frequently they used them. The sample was consisted of 312 housewives living in Daegu, Kyungsan city and Kyungsan-eup, the survey was made by use of questionaires from July 15 to August 1 1989. The results are as follows 1. Analysis of Folk Remedical Contents 1) The method of the Folk Remedies used in this area was mainly food, which can be easily found around their house and easy to use, it was possible to apply scientific principles in some cases. 2) The major popular method was shown to feed boiled barley or brown rice which are known as the Folk Remedy for Diabetes. 3) The ANOVA of variable shows the significant influence on each object which age, religion, the level of education, job, monthly income, and the area except family formation. 2. The Frequency of Folk Remedies and additional analysis 1) The most frequently used Folk Remedy was Arterio-sclerosis(1.44), which is followed by the loss of Eyesight(1.40), Hang over(1.28), Couth(1.27), Cold(1.26) etc. 2) In the analysis of Pearson Correlation between frequencies of Folk Remedies used, and demographic variables such as age(p<.05), the monthly income(p<.01), in that area, total number of response items showed a positive correlation. 3. The source to learn about Folk Remedy. 1) It is acknoledged that they are mostly instructed by their forefathers, friends, neighbors, professional textbooks, mass communications, herb doctors, other medical sources or education at school etc. 2) The ANOVA of Variables shows the big differences between each group by age. Based on the above findings, the following suggestions are made ; Most of Korean Traditional Folk Remedies are recognized scientific and reasonable which are based on the scientific research and herb medicine therefore it should be made good use of for our life in good health additionally. I hereby insist that the importance of our Folk Remedies should be reviewed and focused for maintenance our health.

• Journal of the Korean Society for Library and Information Science
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• v.45 no.1
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• pp.353-376
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• 2011

The goal of this study is to examine what kind of repeated-reading habits adults have, what sort of books they read repeatedly, and what preferences they have depending on their age or gender. A questionnaire was distributed to the students at Lifelong Education Center of Chonbuk National University to survey their repeated reading experiences. The results of the statistical analysis are as follows: 1) Most adults have experienced repeated reading. 2) Women have more experience with repeated reading than men, and in terms of age, more people in their forties read the same books repeatedly. 3) Most repeated readers read their books twice: Women tend to read their books repeatedly more than men do. 4) More than half of the adults in the sample group have read their favorite books several times. 5) The importance of books in this study was judged on the basis of the number of repeated readers and the frequency of reading. The order of important books is as follows: The Little Prince, The Tale of Three Kingdoms, The Giving Tree, The Greek and Roman Mythology, Meu Pe de Larania Lima, No Possession, Hope for the Flowers, and Demian. 6) More than half of the male repeated readers read The Tale of Three Kingdoms while female repeated readers tend to read a wider variety of books. 7) Adults read the same books repeatedly to enjoy and learn something.

• Health Policy and Management
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• v.16 no.4
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• pp.48-67
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• 2006

The aim of the study was to examine levels of satisfaction with health care in North Korea and to identify factors associated with it using a convenience sample of North Korean refugees in China. Data from the 2004 Survey of Health Seeking Behavior of North Korean Households conducted by the Center for Refugee and Disaster Response, Johns Hopkins Bloomberg School of Public Health were used. The study subjects were 273 North Korean refugees whose length of stay in China was less than 3 months. Factor analysis was used to extract factor dimensions from the 12 satisfaction items. Bivariate (t test and ANOVA) and multiple regression analyses were used in examining factors associated with satisfaction with health care use in North Korea Overall, satisfaction level was low ($2.36{\pm}0.36$, score range: 1-5). Of the three-factor dimensions, physician skills scored the highest $(2.93{\pm}0.36)$, followed by drug availability $(2.51{\pm}0.07)$ and general cleanliness $(1.66{\pm}0.55)$. In the multiple regression analysis, having a usual source of care was significantly associated with patient satisfaction. Respondents who identified primary care (section) doctors as their usual source of care tended to be less satisfied than those with the city or county hospital as their usual source of care. County residents tended to report a lower degree of satisfaction with general cleanliness than city residents. Among socioeconomic characteristics, the number of household assets positively predicted satisfaction with drug availability. North Korean residents appear to be dissatisfied with their medical care. It may reflect some inadequacies in the North's universal health care system to meet the healthcare needs of its people.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.11
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• pp.3373-3383
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• 2009

The aim of the current study is to look at the mediating effects of structural features (i.e. decentralization, formalization, and specialization) on the relationship between product variety and the performance of product family. This study investigates the impact of decentralization and formalization for platform and derivative projects separately and in the context of the performance of the product family as a whole, as opposed to individual projects. In addition to relationships between people and groups, the current study considers physical element of an organization such as geographical location in which business tasks are conducted. The current study focuses on spatial differentiation which refers to the number of different sites or locations operated by an organization. Based on a cross-industry sample of 103 Korean manufacturers, this study examines the role of organizational structure features in which firms successfully increase product variety. The study examines that formalization in platform projects and decentralization in derivative projects enhance high variety firms' product family performance. The study finds significant mediating effect of spatial proximity on the relationship between product variety and product family performance.

• Journal of Animal Science and Technology
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• v.50 no.2
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• pp.237-246
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• 2008

Four different pork loaves were made with two levels, HP/FP-3% and HP/FP-6%, containing 3.0 and 6.0% HP(hot air dried sweet persimmon powder)/FP(freezer dried sweet persimmon powder), respectively. HP and FP(3% and 6%) were added into ground pork loin separately. A control without persimmon powder were used for comparison. Patties were cooked to 74℃ in electric oven, after cooling, the aerobic packaged meat loaves were stored at 5℃. The moisture and crude ash were higher in the FP-6%, and the crude protein and crude fat were no different among the samples. The pH in control sample was significantly higher(p<0.05) than those of treatment group, however no significant difference was found among the 4 treatments. The TBARS(thiobarbituric reactive substance) values of all samples showed a tendency of increasing value along the storage days. The TBARS and a* value increased(p<0.05) as the addition ratio of HP/FP increased, respectively. With regard to microorganisms, the number of total aerobic bacteria were lower than 3.11 log10 CFU/g. In sensory evaluation, meat loaves containing persimmon powder resulted in a high overall acceptability, although they were not significantly different in overall acceptability.

• Food Science of Animal Resources
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• v.28 no.5
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• pp.574-579
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• 2008

To develop a yogurt for sensitive consumer such as infants, children or immuno-compromised patients using gamma irradiation, the effects of gamma irradiation on the microbiological, chemical, and sensory quality in a commercial plain yogurt were investigated. No viable coliform bacteria were detected in the sample and approximately a 6 decimal reduction was achieved in the number of total aerobic bacteria by irradiation at 5 kGy. The initial population of lactic acid bacteria observed in the commercial plain yogurt was 8.95 log CFU/g. Gamma irradiation significantly reduced the initial microbial level to 6.47 and 2.85 log CFU/g after irradiation at 1 and 3 kGy, respectively (p<0.05). However, irradiation dose up to 5 kGy could not completely eliminate the lactic acid bacteria in commercial plain yogurt. pH, color, lactose and lactic acid content, and sensory quality were not affected by irradiation treatment and storage. Our results suggest that irradiation can improve the microbial quality of commercial plain yogurt without impairing the physicochemical and sensory quality.