• Korean Journal of Veterinary Research
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• v.39 no.3
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• pp.523-530
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• 1999

Sal enteritidis thin fimbriae, SEF14, were found to be restricted to the predominantly poultry-associated members of the Salmonella serogroup D1 that are considered as the important pathogens in poultry industry. SefA together with sefB and sefC encode the proteins involved in SEF14 biosynthesis. In order to develop the rapid and specific detection methods for Salmonella serogroup D1, a PCR technique for the amplification of sefA gene was established, and its specificity and sensitivity were investigated with various microorganisms. The bacterial genomic DNA was extracted by colony-picking and rapid boiled-lysate technique. In comparison of Sef I and Sef II primers used in the PCR, Sef I primer for sefA gene of 513bp showed higher specificity than that of Sef II. The established PCR was as sensitive as to detect 1pg of Sal enteritidis DNA. When 73 strains in 28 genera including the reference strains and the field isolates of various Salmonella serotypes, Bacillus subtilis, Bordetella bronchisepdca, E coli, Listeria spp., Micrococcus luteus, Rhodococcus equi, Staphylococcus spp., Streptococcus spp., Vibrio parahemolyticus, Yersinia spp. were studied, the established PCR yielded specifically positive results with only Salmonella serogroup D1. The results suggested that the PCR for sefA gene could be a potential candidate among the specific detection methods for Salmonella serogroup D1.

• Proceedings of the Korea Information Processing Society Conference
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• 2007.05a
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• pp.1516-1519
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• 2007

본 연구팀은 유비쿼터스 환경에서 다양한 분야의 콘텐츠를 보다 쉽게 개발하고 실행할 수 있는 통합소프트웨어 개발 솔루션인 유비쿼터스 게임 플랫폼(Ubiquitous Game Platform)을 개발하였다. 유비쿼터스 게임 플랫폼은 유비쿼터스 환경의 기기에 탑재되어 콘텐츠들을 C/C++/Java 언어 모두를 수용하며 가상기계 방식이어서 유비쿼터스 환경의 기기에 독립적으로 실행이 가능하다는 장점을 가지고 있다. 본 논문에서는 유비쿼터스 게임 플랫폼의 가상기계인 유비쿼터스 가상기계(u-VM)의 입력인 SEF(Standard Executable Format) 실행파일을 생성하기 위한 SAF 어셈블러를 설계 및 구현 하였다. SAF 어셈블러는 중간언어 형식인 SAF(Standard Assembly Format)를 입력으로 받아 u-VM이 실행 가능한 파일 형식인 SEF를 생성한다. SEF 파일을 생성하면서 SAF 어셈블러는 여러개의 SAF파일을 한 개의 SEF로 묶어주는 링커의 역할을 하여 주며, 실행 파일을 텍스트 형식의 SAF에서 바이너리 형식의 SEF로 바꿈으로써 보안상의 문제를 해결해 준다. 또한 SEF는 SAF에 비해 가볍게 변환이 되어 가상기계에서의 실행속도를 개선하는 역할을 하여 준다.

• Journal of the Institute of Convergence Signal Processing
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• v.20 no.2
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• pp.63-69
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• 2019

In this study, bispectrum analysis method introduced to reduce variability of SEF(spectral edge frequency) and MF(median frequency), which are the anesthetic depth indexes extracted by EEG spectral analysis. Bispectrum analysis is an analytical method that can confirm the nonlinearity of EEG. Signal measurement and analysis in the surgical environment should take into consideration various external artifact factors. Bispectrum analysis can confirm the presence of externally introduced artifacts, thereby effectively eliminating artifacts that affect the EEG signal. By applying bispectrum parameters, real-time variability of the anesthetic depth parameters SEF, MF could be reduced. Elimination of variability makes it possible to use SEF, MF as a real-time index during surgery.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.553-556
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• 1993

Soybean nuclear extracts were prepared to examine the expression of SEF3 (soybean embryo factors 3), which binds to the upstream region of soybean ${\beta}-conglycinin$ ${\alpha}'$ subunit gene and is presumed to be a trans-acting factor for the expression of the gene. The relative levels of SEF3 binding activity in nuclear extracts of maturing soybean embryos were determined using the SE3 DNA probe containing two AACCCA hexanucleotides for gel mobility shift assay. The SEF3 activity increased in developing embryos from 16 to 32 days after pollination, whereas the mobility of the SE3-SE3-SEF3 complex decreased. The mobility of the complex was increased by the treatment of nuclear extracts with alkaline phosphatese, which could be inhibited by phosphate. Formation of the SE3-SEF3 complex was not affected by the binding buffer pH between 6.8 and 8.5.

• Korean Journal of Food Science and Technology
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• v.24 no.2
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• pp.171-176
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• 1992

Volatile flavor components of Codonopsis lanceolata were extracted by gas co-distillation (GCD), solvent extraction/fractionation (SEF), and headspace sampling (HSS) methods. The extracts were analyzed by dual-capillary gas chromatography-retention index (GC-RI) and gas chromatography-mass spectrometry(GC-MS). The two extracts prepared by SEF and HSS gave more similar fragrance to the Codonopsis lanceolata than the GCD extract. The GC profiles of the SEF and HSS extracts were similar to each other except for differences in peak areas. The extract prepared by SEF gave a sweet note while the extract prepared by HSS gave a green note. The GCD extract began to give a burnt note of herb medicine with prolonged distillation. Rapid extraction of flavor components from Codonopsis lanceolata was possible in several short steps by SEF and HSS methods compared to GCD. GC-MS and GC-RI were used for peak identification. GC-RI was more effective for identification of isomers, and polar FFAP column was more suitable for identification of polar compounds. From Codonopsis lanceolata we identified 35 volatile flavor constituents, 24 of which have not been previously reported by simultaneous distillation extraction method $^{(5)}$. trans-2-Hexanal, cis-3-hexen-1-ol, trans-2-hexen-1-ol, and hexanol were considered key components of the green note and 1-octen-3-ol, the component of the fresh note. Esters, including amyl propionate, seem to be responsible for the sweet note particular to Codonopsis lanceolata.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.547-552
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• 1993

Soybean nuclear extracts and S-100 were prepared to examine the soybean embryo factors which bind to the upstream region of soybean ${\beta}-conglycinin$ ${\alpha}'$ subunit gene. SEF3(soybean embryo factor 3), which is presumed to be a trans-acting factor for the expression of the gene, was detected in gel mobility shift assay using the DNA probe containing two AACCCA hexanucleotides. DNA probe containing CATGCAT or AACACA was used to find any other soybean embryo factor interacting with the upstream region of ${\beta}-Conglycinin$ ${\alpha}'$ subunit gene. It was found that there was no common DNA binding protein detected both in nuclear extracts and S-100. The relative levels of SEF3 binding activity both in nuclear extracts and S-100 of maturing soybean seeds were determined. SEF3 activity of nuclear extracts was first detected around 20 days after pollination and significantly increased around 32 days after pollination.

• Journal of the Korea Institute of Building Construction
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• v.20 no.1
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• pp.93-102
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• 2020

The purpose of this study is to find out how to measure the stress related to accidents at the construction site promptly and conveniently to prevent safety accidents of construction workers. Accordingly, we analyzed the correlations between the questionnaire tool index that measures the stress associated with complex psychology of humans by integrating emotion, cognition, physical and behavioral responses, and basic brain waves, SEF-90, concentration, stress index from brain wave. As a result, which had the highest correlation with the stress measured through the questionnaire, was the SEF-90, and the regression analysis between two independent variables yielded a specific regression equation. This suggests the possibility of measuring the integrated stress of construction workers through the EEG signal at the construction site, and it can be used for the safety management of the construction site in the future.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.6
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• pp.674-682
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• 2014

The purpose of this study was to identify the effect of applying oriental music therapy and reliability of electroencephalogram(EEG) equipment. The study was approved by the critical trial judge committee from ${\bigcirc}{\bigcirc}$(IRB No. 2013-07) university. In order to measure test-retest reliability for 15 subjects, EEG for same participants were measured using same method mentioned above after 2 hours from the first measurement. Same provider implemented to each person at same time. Firstly, EEG was measured for 5 minutes after the subject with attached electrodes sat on chair comfortably for 10 minutes. Then, the subject was given mental stress using the four fundamental arithmetic operations for 5 minutes, and measured EEG for another 5 minutes. After that the subject sat on the chair comfortably listening oriental medicine music therapy for 5 minutes, and EEG was measured for 5 minutes again. There was no side effect regarding music therapy reported. Raw data, which was measured in each step, were converted through FFT(fast fourier transform) and analyzed after divided into certain frequency including ${\alpha}$ wave, ${\beta}$ wave, ${\theta}$ wave, immersion wave, stabilization wave, sef100 wave, and sef95 wave. Data were analyzed using wilcoxon signed rank test, Intraclass correlation coefficient(ICC), repeated measures ANOVA with the SPSS program. In test-retes method, there were significantly differences in ${\alpha}$ wave, ${\beta}$ wave, immersion wave, stability wave, ${\theta}$ wave, sef100 wave, sef95 wave. ICC has shown a high degree of reliability that it was ${\alpha}$ wave .877, ${\beta}$ wave .855, ${\gamma}$ wave .895, immersion wave .897, stability wave .816, ${\theta}$ wave .904, sef100 wave .910, sef95 wave .776. Also, there was a statistically significant difference in ${\alpha}$ wave after applying oriental music therapy. Based on these results, it is considered that average of the channel EEG and application of oriental music therapy would be practiced by increase of sample size using this machine.

• Journal of the Korea Society for Simulation
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• v.20 no.4
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• pp.21-29
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• 2011

Wireless sensor nodes operate in open environments. The deployed sensor nodes are very vulnerable to physical attacks from outside. Attackers compromise some sensor nodes. The compromised nodes by attackers can lead to false data injection into sensor networks. These attacks deplete the limited energy of sensor nodes. Ye et al. proposed the Statistical En-Route Filtering (SEF) as a countermeasure of the attacks. The sensor node in SEF examines the event reports based on certain uniform probability. Thus, the same energies are consumed in both legitimate reports and false reports. In this paper, we propose a method that each node controls the probability of attempts to verify a report to reduce energy consumption of sensor nodes. The probability is determined in consideration of the remaining energy of the node, the number of hops from the node to SINK node, the ratio of false reports. the proposed method can have security which is similar with SEF and consumes lower energy than SEF.

• Applied Biological Chemistry
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• v.38 no.1
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• pp.63-66
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• 1995

Soybean nuclear extracts were prepared to detect SEF3(soybean embryo factor 3), which is presumed to be a trans-acting factor for the expression of the soybean ${\beta}-conglycinin\;{\alpha}'$ subunit gene. To increase the specific activity of DNA probe during labeling with $[{\alpha}-^{32}P]$dATP, dATP was added to a final concentration of 1.1 mM during the chase reaction. It results in approximately four-fold increase of specific activity of the DNA probe. Effects of several modifications in preparation of soybean nuclear extracts were examined. It was found that glycerol is effective to stabilize SEF3 during the preparation of nuclear extracts and polyethylenimine could be used to increase the specific activity of SEF3 in nuclear extracts.