Present study describes a method on the application of efficient tissue culture systems for the micro-propagation of juvenile and mature sawtooth oak(Quercus acutissima). Nodal segments with axillary buds were used as initial explant sources. WPM(Woody Plant Medium) was the best in growth and proliferation of shoot among the media tested. Although the single effect of zeatin revealed on two dorminant shoot elongation with normal growth until the elevation of levels up to 3.0mg/l, BAP($N^6$-benzyl amino purine) usually showed better response than zeatin on shoot multiplication and/or elongation. In addition, the incorporation of BAP and zeatin onto the culture media represents more effectiveness in shoot proliferation and its growth. Optimum concentrations of BAP and zeatin were 0.5 and 0.05~1.0mg/l, respectively. Ninety percent of the proliferated shoots was rooted on half-strength GD (Gresshoff and Doy, 1972) medium containing 0.5mg/l IBA(indole butyric acid) in 4 weeks after culture. More than 70% of the rooted plantlets survived after 5 months of transplanting into artificial soil mix containing equal amount of peatmoss and perlite. Among 27 plus tree clones which were grafted twice onto the juvenile rootstocks, only 4 clones revealed the possibility for shoot multiplication through tissue culture system. The capacity for the micropropagation using mature explant sources was highly depended on clonal differences compared with those of octet age. More than 90% of rooting ratio was obtained from the best responding clone. Among the 7 rooting media tested, GD medium was the best far rooting. The most effective rooting was obtained on half-strength GD medium containing 0.2 to 2.0mg/l IBA. More than 60% of rooted plantlets survived after 5 months of transplanting into the artificial soil mix.
The goals of this study were to present presurgical naso-alveolar molding (PNAM) appliance in unilateral cleft lip and palate treatment and to evaluate the effects of PNAM appliance on alveolar molding. Samples were consisted of 4 unilateral cleft lip and palate infants (3 males and 1 female, mean age=23.2 days after birth) who were treated with PNAM appliances in Department of Orthodontics, Seoul National University Dental Hospital. Average alveolar cleft gap between the greater and lesser segment was 8.27mm and average duration of alveolar molding treatment was 9.7 weeks. These patients' models were obtained at initial visit (T1) and alter successful alveolar molding (T2). Seven linear and five angular variables were measured by using photometry and digital caliper. All statistical analyses were performed by SPSS win ver. 7.5 program. Paired t-test was used to compare the mean values. 1. The posterior part of alveolar segments are the stable structures during alveolar molding treatment period in infants. 2. Forward growth of the greater segment may be hindered by the action of alveolar molding. 3. The closure of cleft gap during alveolar molding were usually due to inward and backward bending of the anterior part of the greater segment and outward bending of the whole lesser segment.
The purpose of this study was to evaluate the light microscopic features and the maximum insertional and removal torque value of microimplants, made from titanium grade 2 or 4, in the tibia of 6 rabbits. First, the maximum torque values of microimplants at implantation were measured. After 2, 8, and 12 weeks of healing time, the microimplant-containing segments of tibia of 2 rabbits were removed and the maximum removal torque of each microimplant were measured. Comparisons of histologic examination and insertional and removal torque values were carried out for the two groups of microimplants. Removal torque values were significantly increased in both groups after 8 and 12 weeks as compared to 2 weeks after implantation. Other values measured did not show any statistically significant differences and there were no histological differences between grade 2 and 4 titanium. Based on these results, this study showed that there were no significant differences between grade 2 and 4 titanium. It seems better to use grade 4 titanium for making microimplants because grade 4 titanium is mechanically harder than grade 2 titanium and has similar retention.
Authors investigated neuronal changes of local cellular metabolism in the cerebral lesions of Parkinsonian symptomatic side between before and after stereotactic neurosurgery by follow-up 1H magnetic resonance spectroscopy (MRS). Patients with Parkinson's disease (PD) (n = 15) and age-matched normal controls (n = 15) underwen MRS examinations using a stimulated echo acquisition mode (STEAM) pulse sequence that provided 2${\times}$2${\times}$2 ㎤ (8ml) volume of interest in the regions of substantia nigra, thalamus, and lentiform nucleus. Spectral parameters were 20 ms TE, 2000 ms TR, 128 averages,2500 Hz spectral width, and 2048 data points. Raw data were processed by the SAGE data analysis package (GE Medical Systems). Peak areas of N-acetylaspartate (NAA), creatine (Cr), choline-containing compounds (Cho), inositols (Ins), and the sum (Glx) of glutamate and GABA were calculated by means of fitting the spectrum to a summation of Lorentzian curves using Marquardt algorithm. After blindly processed, we evaluated neuronal alterations of observable metabolite ratios between before and after stereotactic neurosurgery using Pearson product-moment analysis (SPSS, Ver. 6.0). A significant reduction of NAA/Cho ratio was observed in the cerebral lesion in substantia nigra of PD patient related to the symptomatic side after neurosurgery (P : 0.03). In thalamus, NAA/Cho ratio was also significantly decreased in the cerebral lesion including the electrode-surgical region (P : 0.03). A significant reduction of NAA/Cho ratio in lentiform nucleus was not oberved, but tended toward significant reduction after neurosurgery (P = 0.08). In particular, remarkable lactate signal was noted from the surgical thalamic lesions of 6 among 8 patients and internal segments of globus pallidus of 6 among 7 patients, respectively. Significant metabolic alterations of NAA/Cho ratio might reflect functional changes of neuropathological processes in the lesion of substantia nigra, thalamus, and lentiform nucleus, and could be a valuable finding fur evaluation of Parkinson's disease after neurosurgery. Increase of lactate signals, being remarkable in surgical lesions, could be consistent with a common consequence of neurosurgical necrosis. Thus, IH MRS could be a useful modality to evaluate the diagnostic and prognostic implications fur Parkinsons disease after functional neurosurgery.
Objective : Posterior lumbar interbody fusion (PLIF) is considered to have the best theoretical potential in promoting bony fusion of unstable vertebral segments by way of a load sharing effect of the anterior column. This study was undertaken to investigate the efficacy of PLIF with cages in chronic degenerative disc disease with Modic degeneration (changes of vertebral end plate). Methods : A total of 597 patients underwent a PLIF with threaded fusion cages (TFC) from 1993 to 2000. Three-hundred-fifty-one patients, who could be followed for more than 3 years, were enrolled in this study. Patients were grouped into 4 categories according to Modic classification (no degeneration : 259, type 1 : 26, type 2 : 55, type 3 : 11). Clinical and radiographic data were evaluated retrospectively. Results : The clinical success rate according to the Prolo's functional and economic outcome scale was 86% in patients without degeneration and 83% in patients with Modic degeneration. The clinical outcomes in each group were 88% in type 1, 84% in type 2, and 73% in type 3. The bony fusion rate was 97% in patients without degeneration and 83% in patients with Modic degeneration. The bony fusion rate in each group was 81% in type 1, 84% in type 2, and 55% in type 3. The clinical success and fusion rates were significantly lower in patients with type 3 degeneration. Conclusion : The PLIF with TFC has been found to be an effective procedure for lumbar spine fusion. But, the clinical outcome and bony fusion rates were significantly low in the patients with Modic type 3. The authors suggest that PLIF combined with pedicle screw fixation would be the better for them.
Kim, Woong-Beom;Hyun, Seung-Jae;Choi, Hoyong;Kim, Ki-Jeong;Jahng, Tae-Ahn;Kim, Hyun-Jib
Journal of Korean Neurosurgical Society
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v.59
no.4
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pp.385-391
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2016
Objective : The purpose of this study was to evaluate long-term follow-up radiologic/clinical outcomes of patients who underwent anterior cervical discectomy and inter-body fusion (ACDF) with stand-alone cages (SAC) in a single academic institution. Methods : Total 99 patients who underwent ACDF with SAC between February 2004 and December 2012 were evaluated retrospectively. A total of 131 segments were enrolled in this study. Basic demographic information, radiographic [segmental subsidence rate, fusion rate, C2-7 global angle, and segmental angle changes)/clinical outcomes (by Odom's criteria and visual analog score (VAS)] and complications were evaluated to determine the long-term outcomes. Results : The majority were males (55 vs. 44) with average age of 53.2. Mean follow-up period was 62.9 months. The segmental subsidence rate was 53.4% and fusion rate was 73.3%. In the subsidence group, anterior intervertebral height (AIH) had more tendency of subsiding than middle or posterior intervertebral height (p=0.01). The segmental angle led kyphotic change related to the subsidence of the AIH. Adjacent segmental disease was occurred in 18 (18.2%) patients. Total 6 (6%) reoperations were performed at the index level. There was no statistical significance between clinical and radiological outcomes. But, overall long-term clinical outcome by Odom's criteria was unsatisfactory (64.64%). The neck and arm VAS score were increased by over time. Conclusion : Long-term outcomes of ACDF with SAC group were acceptable but not satisfactory. For optimal decision making, more additional comparative long-term outcome data is needed between ACDF with SAC and ACDF with plating.
The present study was carried out to examine flow properties in linear drainage channels such as road surface drainage facilities. The finite difference formulation for the varied flow analysis was solved for flow profiles in the channels. Starting the first step at the control section, the Newton-Raphson method was applied for producing numerical solutions of the equation. We considered two types of linear drainage channels, a channel with one outlet at downstream end and a channel with two outlets at both ends. Moreover, the flow analysis for various channel slopes was performed. However, we considered channels with the two outlets of slopes satisfying the condition that the both ends are the control section. The maximum of those slopes was decided from the relation between the channel slope and the location of control section. The flow of a channel with one outlet was calculated upward and downward from the control section existing in channel or upward from the control section at downstream end. The flow of a channel with two outlets at both ends were calculated for upstream and downstream channel segments divided by the water dividend, respectively and the flow analysis was completed when the water depth at the water dividend calculated from upstream end was equal to that calculated from downstream end. If the slope was larger than the critical slope, the channel with two outlets was likely to behave like the channel with one outlet. The maximum water depth was investigated and compared with that calculated additionally from the uniform flow analysis. The uniform flow analysis was likely to lead a excessive design of a drainage channel with mild slope.
Korean Journal of Construction Engineering and Management
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v.12
no.3
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pp.42-52
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2011
As environmental issues are rising recently efforts to reduce environmental stress are emerging in all industry segments. Especially environmental impact of buildings occupy a critical portion, so each country is operating green building rating system for life cycle of buildings. Accordingly green building rating system for every facility is operating in Korea. And acquisition of grade I for building energy efficiency is mandatory for every new public buildings since 2010. To design green building efficiently and systematically eco-friendly elements should be considered and checked from the schematic design phase. But in many cases eco-friendly elements are checked at the end of constructed design phase. So applying eco-friendly elements at the value engineering process, which is performing through schematic and constructed design phase, could make a efficient and systematic green building design. Value engineering process is divided into pre workshop, workshop and post workshop stages. And subject selection in pre workshop stage is the step that finds out the subjects which has the great possibility to be improved to perform efficient value engineering workshop. So this study present the Green VE subject selection model to select the most considerable eco-friendly subjects in projects.
Streaming media has contributed to a significant amount of today's Internet Traffic. Like traditional web objects, rich media objects can benefit from proxy caching, but caching streaming media is more of challenging than caching simple web objects, because the streaming media have features such as huge size and high bandwidth. And to support various bandwidth requirements for the heterogeneous ubiquitous devices, a transcoding proxy is usually necessary to provide not only adapting multimedia streams to the client by transcoding, but also caching them for later use. The traditional proxy considers only a single version of the objects, whether they are to be cached or not. However the transcoding proxy has to evaluate the aggregate effect from caching multiple versions of the same object to determine an optimal set of cache objects. And recent researches about multimedia caching frequently store initial parts of videos on the proxy to reduce playback latency and archive better performance. Also lots of researches manage the contents with segments for efficient storage management. In this paper, we define the 9-events of transcoding proxy using 4-atomic events. According to these events, the transcoding proxy can define the next actions. Then, we also propose the segment-based caching policy for the transcoding proxy system. The performance results show that the proposing policy have a low delayed start time, high byte-hit ratio and less transcoding data.
Journal of the Korean Applied Science and Technology
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v.31
no.4
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pp.595-601
/
2014
The microstructures of NATM were examined by SEM, FT-IR spectra, tensile properties, mole % of [NCO/OH], and particle size analyzer. Growing concerns in the environment-friendly industries have led to the development of solvent-free formulations that can be cured. We had synthesized NATM(New Austria Tunnel Method) resin having the ability to protect stainless steel against corrosion. Comparing with general NATM resin and coatings, this resin that synthesized with polyurethane and epoxy was highly stronger in intensity and longer durability. Hybrid resin was composed of polyols, MDI, epoxy, silicone surfactant, catalyst and crosslink agent, and fillers. Moreover, fillers such as fume silica not only accelerated the curing rate but also improved the physical property as thermal barriers. The rigid segments of synthetic resin in mechanical properties were due to fume silica and the increase the mole% of [NCO/OH] for corrosion protection. In conclusion, the hybrid resin microstructure with crosslink agent and fume silica are good material for thermosetting coating of metal substrates such as stainless steel.
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