• Korean Journal of Pharmacognosy
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• v.32 no.1 s.124
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• pp.49-54
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• 2001

The effects of protoberberine compounds on serotonin biosynthesis in P815 cells were investigated. Protoberberine compounds such as berberine, palmatine and coralyne decreased serotonin content dose-dependently, but coptisine did not. The $IC_{50}$ values of berberine, palmatine and coralyne were $3.0\;{\mu}M,\;16.5\;{\mu}M\;and\;14.5\;{\mu}M$, respectively. Protoberberine compounds at concentrations up to $20\;{\mu}M$ were not cytotoxic towards P815 cells. The activity of tryptophan hydroxylase, a ratelimiting enzyme in serotonin biosynthesis, was inhibited by the exposure of berberine, palmatine and coralyne in P815 cells (14.9-19.3% inhibition at $2-15\;{\mu}M$), but that of aromatic L-amino acid decarboxylase was not. These results suggest that the inhibition of tryptophan hydroxylase activity by berberine, palmatine and coralyne might partially contribute to the decrease in serotonin content in P815 cells.

• Journal of the Korean Society of Physical Medicine
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• v.7 no.4
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• pp.451-458
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• 2012

PURPOSE: The purpose of present study was to determine effects of circuit class training (CCT) on the synthesis of central serotonin in people with post-stroke depression. METHODS: Forty patients with mild post-stroke depression were participated in current study. All subjects were assigned to two groups of CCT (circuit class training) group and SW (stretching and weight shifting) group. Both groups were performed for 80 minutes. The change of blood f-Trp, BCAAs, f-Trp/BCAAs and serotonin according to period training time were examined and the following results were obtained. RESULTS: In the CCT group, f-Trp, BCAAs, and f-Trp/BCAAs increased according to the time point. However, after 24 hour of circuit class training, all of these were significantly decreased by those before training. The change in blood levels of serotonin was the highest in immediately after the training in the CCT group while it was not changed significantly in the SW group. CONCLUSION: It can be seen that CCT can change the serotonin level and have an effect on the synthesis of central serotonin in people with post-stroke depression.

• Journal of Nutrition and Health
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• v.35 no.5
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• pp.524-530
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• 2002

To evaluate the role of obesity and nutrient intake on serum IGF-1 and serotonin levels in 80 elementary school children (aged 10. 8 yr, 47 boys, 33 girls), we investigated the anthropometric data and the nutrient intake by questionnaries including food daily record, and measured serum IGF-1 and serotonin using RIA and HPLC F-1050 respectively. We obtained the following results by obesity index (underweight-, normal-, obese group). The protein intake of normal group was higher than the others (p < 0.05). The underweight and obese groups had snacks more often than the normal group. The obese group preferred‘cookies’and‘fries’more than the other groups. The serum serotonin levels showed weak correlation with fat (r = 0.315, p < 0.01), fiber (${\gamma}$ = 0.280, p < 0.05) and energy intake (r = 0.242, p < 0.05), but no differences in anthropometric data by obesity index. The serum IGF-1 level was significantly correlated height (r = 0.649, p < 0.001), weight (r = 0.437, p < 0.001) and hip (r = 0.417, p < 0.001), but showed weak correlation with energy intake (r = 0.232, p < 0.05) and carbohydrate intake (r = 0.244, p < 0.05). In this study, we could see only partly correlation among the serum IGF-1 and serotonin and obesity and nutrient intake. Forker research is required into consideration of the essential role of these hormones during a growth period.

• Journal of Oriental Neuropsychiatry
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• v.21 no.1
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• pp.1-11
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• 2010

Objectives: This study was perfomed to investigate the antioxidant activity and serotonin activity of Gami-guibi-tang on P815 Mast Cell. Methods: The effects of Gami-guibi-tang on activation of TPH-1 mRNA and AAADC mRNA in P815 mast cell were investigated. The effect of Gami-guibi-tang on content of serotonin in P815 mast cell was investigated. The effects of Gami-guibi-tang on activation of DPPH radical scavenging and SOD in P815 mast cell were investigated. Results: 1. The Gami-guibi-tang increased SOD activity and DPPH radical scavenging activity. 2. The Gami-guibi-tang increased the intracellular concentration of serotoninin 60 ${\mu}g/ml$, 80 ${\mu}g/ml$ experiment group. 3. The Gami-guibi-tang increased menaingful the manifestation TPH mRNA. 4. The manifestation of AAADC and MAO mRNA have not made menaingful changes on Gami-guibi-tang. Conclusions: This experiment shows that Gami-guibi-tang had significant anti-oxidative effect. And Gami-guibi-tang increased the intracellular concentration of serotonin. Therefore, Gami-guibi-tang can be used by the medication of major depression disorder. But Study on mechanism of increased serotonin and clinical research of Gami-guibi-tang is suggested for future research.

• The Journal of Dong Guk Oriental Medicine
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• v.3
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• pp.91-106
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• 1994

This Study was performed to prove the effects of Gungsindodamtang (GDT) and Dangquibohyultang (DBT) on the cosntents of Serotonin and Catecholamine in the Brain and the Plasma of the Reserpine treated rats. High-Perfomance Liquid Chromatography was used for measuring the contents of the Serotonin and Catecholamine. The results were as follows. 1. norepinephrine contents in the Brain were increased significantly in GDT-treated group and DBT-treated group in comparison with the control group. 2. epinephrine and serotonin contents in the Brain were increased in all the sample groups but have not significance. 3. norepinephrine contents in the plasma were increased significantly in all the sample groups in comparison with the control group. 4. epinephrine contents in the plasma were increased in all the sample groups but have not significance. 5. serotonin contents in the plasma were increased significantly in DBT-treated group in comparison with the control group, and inreased in GDT-treated group but have not significance. According to the above results, it is considered that Dangquibohyultang could be applied more effectively than Gungsindodamtang in decrease of the serotonin and catecholamine and other symptoms induced by Reserpine.

• YAKHAK HOEJI
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• v.51 no.2
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• pp.83-87
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• 2007

The effects of ethaverine on serotonin biosynthesis in murine mastocytoma P815 cells were investigated. Ethaverine at 2.5${\sim}$10 ${\mu}$M decreased serotonin content in a concentration-dependent manner. The IC$_{50}$ value of ethaverine was 6.1 ${\mu}$M. Ethaverine at concentrations up to 20 ${\mu}$M was not cytotoxic towards P815 cells. Under these conditions, tryptophan hydroxylase (EC 1.14.16.4; TPH), a rate-limiting enzyme in serotonin biosynthesis, was inhibited by ethaverine in P815 cells (15.3% inhibition at 7.5 ${\mu}$M), however, aromatic L-amino acid decarboxylase (EC 4.1.1.28) was not. These results indicate that ethaverine leads to a decrease in serotonin content by reducing TPH activity in P815 cells.

• Research in Community and Public Health Nursing
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• v.31 no.4
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• pp.472-480
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• 2020

Purpose: This study identified the effects of chronic disease number, quality of sleep and, serum serotonin concentration on the elderly's depression in order to provide basic data for the development of intervention program to effectively prevent and manage depression of the elderly living in the community. Methods: The study surveyed 207 people aged 65 or older living in the community. The general characteristics of the subjects, the number of chronic diseases, the quality of sleep (PSQI-K), the hematological test (Serotonin) and the depression (K-GDS) were statistically analyzed using SPSS/WIN 23.0. Results: It was shown that the elderly's depression differed significantly depending on the number of chronic diseases, the quality of sleep, and serum serotonin concentration, and that the variable that most affected depression was the quality of sleep. Next, the number of chronic diseases and serum serotonin levels were the variables affecting the depression of the elderly. Conclusion: In order to prevent and manage the depression of the elderly in the future, treatment and education for diseases and depression should be provided to the subjects. In particular, nursing interventions are needed to improve the quality of sleep for the elderly. Health care programs should also be provided, such as providing nutritional supplements to provide serotonin and increasing daytime activities.

• Korean Journal of Clinical Pharmacy
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• v.32 no.4
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• pp.313-320
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• 2022

Background: Serotonin syndrome is a life-threatening disease if not appropriately treated. This study aimed to investigate the prescription status of contraindicated drug combinations that cause serotonin syndrome and identify the related factors. Methods: A cross-sectional study was conducted using nationwide claims data. Adult patients taking serotonergic drugs with Parkinson's disease or mental disorders were selected. Based on international medical databases (MDBs) and the Korean Drug Utilization Review (DUR), the status of prescribing contraindicated drug combinations that induce serotonin syndrome, the related factors, and the difference between international MDBs and the Korean DUR were analyzed. Results: Of the 49,773 study subjects, 163 (0.3%) were prescribed contraindicated serotonergic drug combinations based on international MDBs, and among them, only 105 (64.4%) were contraindicated by the Korean DUR. Positive influencing factors for prescribing contraindicated drug combinations include patient age between 65 and 74 and physician's specialties (neurologists, and orthopedists). Negative influencing factors were physician's specialty (internists) and medical institution (primary institutions). Conclusion: Despite the implementation of DUR, 3 out of 1,000 study subjects received contraindicated drug combinations that caused serotonin syndrome. Hence, it is necessary to comply with the DUR and improve it in accordance with international MDBs.

• BMB Reports
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• v.56 no.10
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• pp.527-536
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• 2023

Serotonin receptors, also known as 5-HT receptors, belong to the G protein-coupled receptors (GPCRs) superfamily. They mediate the effects of serotonin, a neurotransmitter that plays a key role in a wide range of functions including mood regulation, cognition and appetite. The functions of serotonin are mediated by a family of 5-HT receptors including 12 GPCRs belonging to six major families: 5-HT₁, 5-HT₂, 5-HT₄, 5-HT₅, 5-HT₆ and 5-HT₇. Despite their distinct characteristics and functions, these receptors' subtypes share common structural features and signaling mechanisms. Understanding the structure, functions and pharmacology of the serotonin receptor family is essential for unraveling the complexities of serotonin signaling and developing targeted therapeutics for neuropsychiatric disorders. However, developing drugs that selectively target specific receptor subtypes is challenging due to the structural similarities in their orthosteric binding sites. This review focuses on the recent advancements in the structural studies of 5-HT receptors, highlighting the key structural features of each subtype and shedding light on their potential as targets for mental health and neurological disorders (such as depression, anxiety, schizophrenia, and migraine) drugs.

• Korean Journal of Veterinary Research
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• v.41 no.2
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• pp.133-138
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• 2001

The distributions and relative frequencies of the serotonin-immunoreactive cells were studied in dorsal, ventral, third and splenic lobes of developing chicken pancreas during embryonic periods (10 days of incubation to hatching) by immunohistochemical methods. The regions of pancreas were subdivided into three regions, exocrine, light and dark islets. Round and/or oval shaped serotonin-immunoreactive cells were detected in all four lobes. According to developmental stages, the types of lobes and the regions of pancreas, these immunoreactive cells were showed various distributions and relative frequencies. In exocrine portions, serotonin-immunoreactive cells were found in the splenic lobes at 13-14 days of incubation, in the third lobes from 10 days to 19 days of incubation, in the ventral lobes from 10 days of incubation to hatching and in the dorsal lobes from 11 days of incubation to hatching. In pancreatic islets, these cells were detected only in the dark islets of splenic lobes at 15 and 16 day of incubation with rare frequency. In conclusion, serotonin-immunoreactive cells decreased with developmental stages in all four lobes and their relative frequencies decreased with developmental stages.