• Title/Summary/Keyword: standard culture methods

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Comparison of Culture, Conventional and Real-time PCR Methods for Listeria monocytogenes in Foods

  • Kim, Dong-Hyeon;Chon, Jung-Whan;Kim, Hyunsook;Kim, Hong-Seok;Choi, Dasom;Kim, Young-Ji;Yim, Jin-Hyeok;Moon, Jin-San;Seo, Kun-Ho
    • Food Science of Animal Resources
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    • v.34 no.5
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    • pp.665-673
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    • 2014
  • We compared standard culture methods as well as conventional PCR and real-time PCR for the detection of Listeria monocytogenes (L. monocytogenes) in milk, cheese, fresh-cut vegetables, and raw beef that have different levels of background microflora. No statistical differences were observed in sensitivity between the two selective media in all foods. In total, real-time PCR assay exhibited statistically excellent detection sensitivity (p<0.05) and was less time consuming and laborious as compared with standard culture methods. Conventional culture methods showed poor performance in detecting L. monocytogenes in food with high levels of background microflora, generating numerous false negative results. While the detection of L. monocytogenes in fresh cut vegetable by culture methods was hindered only by L. innocua, various background microflora, such as L. innocua, L. welshimeri, L. grayi, and Enterococcus faecalis appeared on the two selective media as presumptive positive colonies in raw beef indicating the necessity of improvement of current selective media. It appears that real-time PCR is an effective and sensitive presumptive screening tool for L. monocytogenes in various types of foods, especially foods samples with high levels of background microflora, thus complementing standard culture methodologies.

Standard Methods for the Detection and Assessment of Safety in Milk and Dairy Products in Korea (우유 및 유제품의 안전성 평가를 위한 병원성미생물 검사법)

  • Kim, Hyoun-Wook;Seol, Kuk-Hwan;Ham, Jun-Sang;Jang, Ae-Ra;Kim, Dong-Hun;Oh, Mi-Hwa
    • Journal of Dairy Science and Biotechnology
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    • v.29 no.2
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    • pp.59-68
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    • 2011
  • In Korea, there are a couple of risk assessment organizations: The Animal Plant and Fisheries Quarantine and Inspection Agency(QIA) and the Korea Food and Drug Administration (KFDA). The major food laws include the Food Sanitation Act (FSA), the Livestock Product Processing Act (LPPA), and the Agricultural Products Quality Control Act (APQCA). Milk and dairy products are mostly controlled by the Food Sanitation Act and the Livestock Product Processing Act. This study was carried out to estimate the current standard methods of foodborne pathogens for dairy products, comparing the Livestock Products Processing Act with the Food Sanitation Act. The standard methods of foodborne pathogens for dairy products are composed by growth culture, isolation culture, and identification, however, standard methods of QIA and KFDA are different at the using of medium and inspection stage. Therefore, consolidation of risk management and risk assessment methods are regarded important to provide safe dairy products to consumer.

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The Effects of Organizational Culture for Infection Control and Self-Efficacy on Compliance with Standard Precautions of Emergency Room Nurses (응급실 간호사의 감염관리 조직문화와 자기효능감이 표준주의 지침 수행에 미치는 영향)

  • Kim, Hyunhee;Park, Hyung-Ran
    • Journal of Korean Biological Nursing Science
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    • v.21 no.1
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    • pp.46-53
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    • 2019
  • Purpose: This study was conducted to identify the effects of organizational culture for the infection control and self-efficacy on compliance with standard precautions of emergency room nurses. Methods: This cross-sectional descriptive study was conducted from 1st July to 3rd August, 2017. The participants were 200 nurses working in emergency rooms. The data was collected using structured self-report questionnaires. Results: The average scores for the organizational culture for infection control, self-efficacy and compliance with standard precautions were 5.54, 3.65, and 4.31, respectively. The organizational culture for infection control (r = .59, p< .001) and self-efficacy (r= .28, p< .001) were found to have a positive, meaningful correlation with compliance with the standard precautions. Multiple regression analysis showed that compliance with standard precautions was significantly affected by the organizational culture for infection control (${\beta}=0.55$, p< .001) and self-efficacy (${\beta}=0.13$, p= .033). Conclusion: The results for this study indicate that the organizational culture for infection control and self-efficacy were highly influential factors affecting compliance with standard precautions of emergency room nurses. Therefore, in order to improve the nurses' use of the standard precautions, it was necessary to develop and evaluate a program that considers the environmental factors and self-efficacy.

Studies on the Water Consumption and Growth of Vegetables Cultivated by Hydroponics in' the Green House (시설채소의 수경재배방법별 소비수량과 생육에 관한 연구)

  • 김시원;이경희;김유현;김선주;임창영
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.31 no.1
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    • pp.31-44
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    • 1989
  • In the vinyl house cultivation, the water consumption, evapotnanspirafion ratio, growth condifion by the growing stages and yields of tomato and cucumber were investigated when they cultivated by nutriculture, rice hull charcoal culture and NFF culture. The results obtained are summarized as follows: 1. The mean air temperature in the vinyl house during the experimental perica j was 1.9$^{\circ}$C higher than the outdoor air temperature, the rrnocirnum and minimum air temperature in the vinyl house was 4.6C and 1.7$^{\circ}$C higher than the outdoor temperature, respectively, and the mean daily evaporation in the vinyl house during the experimental period was 4.3rnm that is 02rnrn more than the outdoor evaporation 2. In the tomato cultivation, the growth of rice hull charcoal culture was the best among the three methods, and the growth of NFT culture and nutriculture were worse than the soil cultivation which is standard, and among the nutriculture, the growth of EC 1.3m.mho plot was the best. 3. In the cucumber cultivation, the growth of rice hull charcoal culture was the best, and while the growth condition of NFT culture was worse than the standard soil cultivation, the growth of nutriculture was better than the standard soil cultivation, and the growth of EC 1.3m.mho plot was the highest among the different treatments in the nutriculture. 4. In the tomato cultivation, the total water consumption of rice hull charcoal culture plot was 1107.5rnm which is the highest amount among the three methods, and in the nutriculture, the total water consumption of EC 1.3m.mho plot was 22lAmm which is the highest among the three different treatments. 5. In the cucumber cultivation, the total water consumption of rice hull charcoal culture was 11762rnm which is the highest, and the total water consumption of EC 1.3m.mho plot was 284.9rnm which is more than the 278.9mm of EC 1.Smmho plot and 262.9mm of EC 1.7rnmho plot. 6. The crop coefficient(Kc) of tomato was 0.82 in NFT culture, 4.67 in rice hull charcoar culture and 0.86~0.91 in nutriculture. 7. The crop coefficient(Kc) of cucumber was higher than tomato as 1.13 in NFT culture, 520 in rice hull charcoal culture and 1.08~1.19 in nutriculture. 8. The evapotranspiration ratio in the mid and late season were higher than the beginning and elongation stage, and the average evaportranspiration ratio of tomato and cucumber was 3.81 and 424, respectively, in the rice hull charcoal culture plot. 9. In the tomato cultivation, the total yield per plant of rice bull charcoal culture was 1443.Og which is the highest, and in the nutriculture, their yields were worse because of the damage of downy nidew disease. 10. In the cucumber cultivation, the total yield per plant of rice hull charcoal culture was 1965.7g which is the highest, and while the yield of NFT culture was ahout 25% lower than the stadard soil cultivation, the yield of nutriculture was higher than the standard soil cultivation, and among the treatments in the nutriculture, the yield of EC 1.3m.mho plot was the highest.

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On the Regulation for Pronunciation of Loanwords in Korean (외래어의 표준 발음과 어문 규범)

  • Yi, Eun-gyeong
    • Cross-Cultural Studies
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    • v.38
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    • pp.405-431
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    • 2015
  • The purpose of this paper is to investigate how to decide pronunciation of loanwords in Korean language. There has not been a regulation for pronunciation of loanwords in Korean language. Even the dictionary published by the government does not provide any information about the pronunciation of loanwords. In this paper, some actual solutions are suggested for the pronunciation of loanwords. Korean language has Regulations of Standard Korean, Korean Orthography, Regulations on Hangeul Transcriptions on Loanwords and Pronunciation Methods of Standard Korean. These language standards could help to decide pronunciation of loanwords. Some pronunciations which could not be regulated by them must be presented in the standard pronunciation dictionary. For example, glottalization rule of 's' in many loanwords could be presented in the description of each loanword in the dictionary. However the pronunciation of loanwords must be similar to the spelling. If various pronunciations are allowed to one spelling, then people will be so confused by the discrepancy between pronunciation and spelling of loanwords.

Freshness and Antioxidant Activities in Pacific Oyster Crassostrea gigas Using Rack-and-Bag Culture or Suspended Culture Methods (부유망식과 수하식 양성방법에 따른 참굴(Crassostrea gigas)의 선도와 항산화활성)

  • Choi, Yong-Jun;Nguyen, Thanh Tri;Lee, Jeong-Mee;Kang, Seok-Joong;Choi, Byeong-Dae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.5
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    • pp.500-505
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    • 2017
  • The nucleotides and their related compounds, including ATP (adenosine triphosphate), ADP (adenosine diphosphate), AMP (adenosine monophosphate), IMP (inosine monophosphate), HxR (inosine) and Hx (hypoxanthine), were nearly identical in oysters Crassostrea gigas from the two culture methods. The K-value was lower than the threshold value such as 11.2-12.1. Although oysters have low amount of IMP, it was detected in this experiment. DPPH radical scavenging activity did not vary significantly with sample amounts (100, 300, and $500{\mu}g/mL$). DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity was 76.0-80.7% compare with the ascorbic acid standard. Superoxide anion scavenging activity reached 49.3% in the rack-and-bag culture sample at $500{\mu}g/mL$. However, the reducing power and $Fe^{2+}$ chelating activity were very low compared with their respective standard. The oyster culture methods did not affect oyster quality in terms of antioxidant activities.

Awareness and Correlation of Patient Safety Culture and Standard Precautions among Health Care Personnel (의료종사자의 환자안전문화와 표준주의 인식과 상관관계)

  • Kwon, Hye-kyung;Jeong, Jae-sim;Lee, Bok-im;Kim, Jang-han
    • Quality Improvement in Health Care
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    • v.21 no.2
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    • pp.58-72
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    • 2015
  • Objectives: This study was conducted as descriptive correlation research in order to survey healthcare personnel(HCP)'s awareness of patient safety culture and their recognition of standard precautions, and to examine the correlation between the two factors. Methods: The subjects were 400 HCPs including 80 doctors, 240 nurses, and 80 medical technicians from two general hospitals. The questionnaire used in the survey consisted of 9 questions on general characteristics, 44 on the perception of patient safety culture, and 21 on the recognition of standard precautions. Results: According to the subjects' general characteristics, the score was significantly higher in those aged over 40 than in those aged 30-39. In addition, it was significantly higher in managers than in practitioners, in those with work experience of less than a year than in those with 5-9 years. The score was also significantly higher in those working 8 hours a day than in those working over 10 hour a day. The number of medical accident reports according to the subjects' general characteristics was significantly larger in nurses than in doctors, in managers than in practitioners, and in those with 10 years' or longer experiences than in those with less than a year. In the awareness of standard precautions according to the subjects' general characteristics, the score was significantly higher in female workers than in male workers, and in managers than in practitioners. As a whole, the subjects' awareness of patient safety culture and their recognition of standard precautions showed a significant correlation with each other. Conclusion: From the result of this study, sufficient work force and the promotion of organizational culture for safety is needed in order to guarantee patient safety. Likewise, these results suggest that experience, job skill, and adequate working hours have a positive effect on the awareness of patient safety culture and the recognition of standard precautions.

A Study on the Quality Control for the Circulation Steps including Productipn, Transportation, Selling about Hamburger & Sandwich in Convenience Store (편의점에서 판매되는 햄버거와 샌드위치의 유통과정중 품질관리에 관한 연구)

  • Kim, Heh-Young;Song, Yong-Hye
    • Journal of the Korean Society of Food Culture
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    • v.11 no.4
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    • pp.465-473
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    • 1996
  • The purpose of this study was to evaluate microbiological hazards in the steps of production, transportation and selling of hamburger and sandwich that were marketed in CVS, then to identify methods of control. The reasults are as follows: As the reasult of operation surroundings of manufacturerand reserch of circulation, $4{\sim}6$ hours are needed from manufacturer to CVS. Also transportation car mean temperature was $10^{\circ}C$ which exceeds the standard of $7^{\circ}C$ or below. Hamburger: Critical control points identified were purchasing, cooking, post-preparation, transportation and holding at CVS. As the reasult of microbial analysis following the case of holding methods and reheating at CVS, microbes of cold holding and reheat after cold holding was within standard value. But in the case of room temperature microbes exceeded standard value. Sandwich: Critical control points identified were purchasing, cooking, post-preparation, transportation and holding at CVS. As the reasult of microbial analysis following the case of holding methods and reheating at CVS, total plate counts of cold holding and reheat after cold holding was within standard value. But in the case of room temperature holding after 24 hours total plate counts exceeded standard value. In the case of room temperature holding the number of microbes increased according to the passage of time. As a reasult of food poisoning bacteria, it was negative in every test in sample against V. parahaemolyticus, Salmonella, S. aureus.

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A Case Study of Fashion Marketing Research using Multiple Methods (마케팅 리서치에서 다중측정방법에 관한 실증적 연구)

  • 박혜정;김혜정;이영주;임숙자
    • The Research Journal of the Costume Culture
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    • v.10 no.6
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    • pp.601-616
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    • 2002
  • Qualitative research is a method widely used in marketing research. However, the method has seldom been used in fashion marketing research in Korea. The purpose of this study was to prove that using both qualitative and quantitative research methods in main stage is much useful than using qualitative research method only in exploratory stage. Qualitative data were gathered by conducting Focus Group Interview(FGI) with 48 college students. Quantitative data were gathered by surveying college students, and 487 questionnaires were used in the statistical analysis. The data were analyzed using content analysis, mean, standard deviation, and t-test. As a result, FGI, one of the tools used in qualitative research methods, was proved to be useful in revealing consumers´deep emotional needs as well as purchase motives. FGI also revealed information which quantitative research method tools such as survey could have missed. Therefore, it is best to use multiple methods-simultaneous use of quantitative and qualitative methods-to understand fast changing consumers´needs and purchase motives.

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A Comparison of Standard Methods for Evaluating the Water Resistance of Shell Fabrics

  • Kwon, Myoung-Sook;Nam, Youn-Ja
    • The International Journal of Costume Culture
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    • v.4 no.3
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    • pp.241-248
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    • 2001
  • Re water resistance of shell fabrics intended for we in outdoor apparel was measured using three different standard test methods, ASTM D 751, hydrostatic resistance, procedure A(Mullen test -- with and without a fabric support) and Procedure B (Hydrostatic head test). A database of information on their water resistance performance was created. The data collected with different methods were correlated and the advantages and disadvantages of each method were compared. The Mullen test with a support appears to give higher and more favorable water resistance values on shell fabrics preventing fabric rupture during the test. The hydrostatic head test gave lower hydrostatic pressure values than those measured on the two Mullen tests. The Mullen test is recommended for testing the water resistance of fabrics that high a relatively high water resistance because the Mullen tester applies a wide range of pressure. The hydrostatic head test is recommended for testing the fabrics that have relatively low water resistance. The area of the fabric sample that is in contact with the water is smaller in the Mullen test, so higher pressure levels can be reached and more samples should probably be tested to get a representative value for each fabric types. Furthermore, the hydrostatic head test was deemed more repeatable than the Mullen tests in his study.

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