• The Plant Pathology Journal
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• v.28 no.2
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• pp.202-206
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• 2012

Root colonization by the rhizobacterium Pseudomonas chlororaphis O6 in Arabidopsis thaliana Col-0 plants resulted in induced tolerance to drought and salinity caused by halide salt-generated ionic stress but not by osmotic stress caused by sorbitol. Stomatal apertures decreased following root colonization by P. chlororaphis O6 in both wild-type and ABA-insensitive Arabidopsis mutant plants. These results suggest that an ABA-independent stomatal closure mechanism in the guard cells of P. chlororaphis O6-colonized plants could be a key phenotype for induced systemic tolerance to drought and salt stress.

• Research in Plant Disease
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• v.24 no.3
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• pp.242-247
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• 2018

Pseudomonas chlororaphis O6 induces systemic tolerance in plants against drought stress. A volatile, 2R, 3R-butanediol, produced by the bacterium causes partial stomatal closure, thus, limiting water loss from the plant. In this study, we report that applications of extracellular polymeric substances (EPS) from P. chlororaphis O6 to epidermal peels of leaves of Arabidopsis thaliana also reduce the size of stomatal openings. Growth of A. thaliana seedlings with applications of the EPS from P. chlororaphis O6 reduced the extent of wilting when water was withheld from the plants. Fluorescence measurements showed photosystem II was protected in the A. thaliana leaves in the water stressed EPS-exposed plants. These findings indicate that P. chlororaphis O6 has redundancy in traits associated with induction of mechanisms to limit water stress in plants.

• Journal of Korean Society of Forest Science
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• v.63 no.1
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• pp.28-34
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• 1984

This study was to elucidate the relation between the water relations parameters obtained from P-V curves and stomatal closure. The results obtained are as follows: 1) The water potential at incipient plasmolysis obtained from P-V curves was similar to the water potential at critical stomatal closure. 2) The critical stomatal closure of sun leaves appear at -21 bar (-17 bar, shade leaves) in Pinus koraiensis, -20 bar in Pinus rigida, -22 bar in Pinus densiflora, and -24 bar in Larix leptolepis. On a relative water content basis, the critical stomatal closures of sun leaves appear at 85% (82%, shade leaves) in Pinus kordiensis, 77% in Pinus ragida, 85% in Pinus densiflora, and 70% in Larix leptolepis. 3) The incipient stomatal closures of sun leaves appear at -14 bar (-12 bar, shade leaves) in Pinus koraiensis, -10 bar in Pinus rigida, -15 bar in Pinus densiflora, and -6 bar in Larix leptolepis. On a relative water content basis, the incipient stomatal closures of sun leaves appear at 90% in Pinus koraiensis, 93% in Pinus rigida, 90% in Pinus densiflora, and 93% in Larix leptolepis. 4) The leaf conductance was increased by increase in volume-averaged turgor pressure was linearly increased by increase in relative water content.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.1
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• pp.33-38
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• 1985

This experiment was carried out to study in stomatal behaviour of wheat (Chokwang) upon the different soil water potentials, lodging and two different nitrogen treatment. Stomatal aperture was measured at flowering stage under diurnal course with two hour intervals, Stomatal apertures were differently changed according to soil water potentials and leaf position on the stem, showing the rapid closure of stomata at low soil water potential and the narrow opening at low leaf position of stem. Wider stomatal opening appeared at the plot of optimal nitrogen application than that of low nitrogen. It was greatly decreased in opening of stomata at plants lodged, appearing that plant investigated of six days after lodging showed less than 50% than that normal plants, which indicated peculiar decrease of low leaf position of stem at afternoon of diurnal course.

• Journal of Plant Biology
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• v.29 no.1
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• pp.41-51
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• 1986

The plants of Nicotiana tabacum L. cv. NC2326 were germinated in 10 cm D$\times$20 cm H polyethylene pot, and sand-cultured with Hoagland solution near by the window of laboratory room(26$\pm$5$^{\circ}C$). The growing plants were sprayed with various concentrations of ABA around 9 : 00 a.m. once in every two days for 12 weeks in summer. As the results, frequency of stomatal number, stomatal opening, chlorophyll content, respiration rate, and protein content in the leaves were decreased with the increasing of concentrations of ABA, respectively. The plant growth was inhibited by exogenous ABA, but leaf abscission was not found during the experimental period. The ratio of three to one in chlorophyll a to b was not altered by exogenous ABA. All the stomata were closed within three minutes by 100 $\mu\textrm{g}$ ml-1 ABA and within seven minutes by 1-10 $\mu\textrm{g}$ ml-1 ABA after the spraying of ABA, and then reopended after a few hours in 1-10 $\mu\textrm{g}$ ml-1 ABA and after 24 hours in 100 $\mu\textrm{g}$ ml-1 ABA. The polar movement of chloroplast within the guard cells was found in the higher concentrations of 10 and 100 $\mu\textrm{g}$ ml-1 ABA, but not found in the lower concentrations than 1 $\mu\textrm{g}$ ml-1 ABA. During the night and weak light, it was fond that the inhibition of respiration rate by the higher concentration of ABA was owing to firstly the stomatal closure by the spraying of ABA and secondly the decrease of stomatal frequency by the inhibition of stomatal development with exogenous ABA for the long period of 12 weeks. In the band number of leaf protein by the electrophoresis, most of the protein bands were disappeared by the higher concentration of 100 $\mu\textrm{g}$ ml-1 ABA, but were not altered by the lower concentration of ABA in comparison with control.

• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.1
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• pp.11-17
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• 2004

This study was conducted to determine the relationship of stomatal responses, photosynthesis, and intercellular $CO_2$ concentration( $C_{i}$) of Betula Species to ozone exposure. Five Betula Species(B. costata, B. davurica, B. schmidtii, B. platyphylla var, iaponica and B, ermani) were grown in the greenhouse. One-year-old potted seedlings of the five Betula Species were exposed to ozone(100 pub) for 8 hours da $y^{-1}$ for 5 weeks in a fumigation chamber. Net photosynthesis was significantly different among species and treatments from early in the period of the fumigation. Stomatal conductance and transpiration rate differences among species and treatments became significant after three weeks of fumigation. $C_{i}$ was significantly different only among treatments; $C_{i}$ of four species, except for B. davurica, was higher than that of control plants. Carboxylation efficiency and photo-respiration rate were significantly different among species or treatments; carboxylation efficiency and photo-respiration rate of the five Betula Species were decreased by ozone treatment. It was concluded that stomatal closure of Betula Species may be the result of the reduction of photosynthesis and rubisco activity and the resulting increase of $C_{i}$. The higher $C_{i}$ likely resulted from reduced photosynthesis because of physiological processes.ocesses.

• Korean Journal of Environmental Biology
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• v.17 no.3
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• pp.359-364
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• 1999

The effect of Salicylic acid (SA) on the leaf growth, chlorophyll content, photosynthesis, stomatal conductance in Commelina communis L. was investigated. C. communis which was grown in Hoagland solution containing 1 mM SA during 3 weeks resulted in a significant reduction of leaf length, width and area. 1 mM SA reduced about 10% of the leaf area at 1 week and 2 weeks, but inhibited 22% of the leaf area at 3 weeks. SA also showed great effect on the reduction of chlorophyll content. SA reduced 47% and 53% of chlorophyll content each at 2 weeks and 3 weeks when it was compared with the control. Chlorophyll a/b ratio in SA treated sample was increased at 3 weeks representing that SA was sensitive to chlorophyll b. The treatment of SA did not inhibit the activity of PSII＋PSI, PSII and PSI. This result indicates that SA did not show direct effect oil the photosynthetic electron transport activity. However, when C. communis was grown in Hoagland solution containing I mM SA fer long period, SA showed clear inhibition of photosyhthesis in intact leaves. The treatment of SA for 3 weeks showed about 23~65% inhibition of photosynthetic activity at various light intensity (100~1000$\mu$mo1 m­$^2$S­$^1$). Similar effect was found on stomatal conductance. The treatment of SA caused an almost closure of stomata. Similar effects of stomatal conductance at various light intensity indicate a loss of normal control on stomatal mechanism. These results indicate that the effect of SA on photo-synthetic activity was not by SA itself but by indirect metabolic pathway.

• The Korean Journal of Ecology
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• v.18 no.2
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• pp.255-263
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• 1995

Two poplar clones, I-214 (Populus euramericana) and Peace (P. Koreana × P. trichocarpa), were grown for 21 days in growth chambers at different CO₂concentrations (350, 700 and 2,000 μL·L-1). I-214 has stomata responding to environmental conditions in normal ways and Peace has unresponsive stomata to environmental factors including light, ABA, water stress and CO₂. In both plants, elevated CO₂ stmulated the growth of plant parts, especially leaf dry weight. And a CO₂ enrichment of 700 μL·L-1 CO₂ caused increment of net assimilation rate (NAR). The growth responses of these plants to CO₂ enrichment were different especially at high CO₂ condition (2,000 μL·L-1 CO₂). The total dry weight in Peace increased up to 2,000 μL·L-1 CO₂ but not in I-214. A CO₂ enrichment of 2,000 2,000 μL·L-1 CO₂ had little effect on NAR of I-214 but enhanced NAR of Peace. Although it is uncertain whether the different responses to CO₂ enrichment between I-214 and Peace resulted from the different properties of stomatal responses to long-term CO₂ treatment, the decrease in NAR is probably due in part to CO₂-induced stomatal closure in I-214 but not in Peace.

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.4
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• pp.143-150
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• 2009

Deicing salt is used to melt snow and ice on the road for traffic safety during the winter season, which accumulates in the roadside vegetation and induces visible injuries. The damage may accelerate particularly when it coincides with early spring leaf out. In order to better understand the response mechanisms, C. kousa (3-year-old) was irrigated twice prior to leaf bud in a rhizosphere with solutions of 0.5, 1.0, and 3.0% calcium chloride ($CaCl_2$) concentration, that were made by using an industrial $CaCl_2$ reagent practical deicing material in Seoul. Physiological traits of the mature leaves were progressively reduced by $CaCl_2$ treatment, resulting in reductions of total chlorophyll contents, chlorophyll a:b, photosynthetic rate, quantum yield, stomatal conductance, $F_V/F_M$, and NPQ. On the contrary, light compensation point and dark respiration were increased at high $CaCl_2$ concentration. A decrease in intercellular $CO_2$ concentration by stomatal closure first resulted in a reduced photosynthetic rate and then was accompanied by low substance metabolic rates and photochemical damage. Based on the reduction of physiological activities at all treatments ($CaCl_2$ 0.5%, 1.0%, and 3.0%), C. kousa was determined as one of the sensitive species to $CaCl_2$.

• The Plant Pathology Journal
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• v.29 no.4
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• pp.427-434
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• 2013

2R,3R-Butanediol, a volatile compound produced by certain rhizobacteria, is involved in induced drought tolerance in Arabidopsis thaliana through mechanisms involving stomatal closure. In this study, we examined the involvement of nitric oxide and hydrogen peroxide in induced drought tolerance, because these are signaling agents in drought stress responses mediated by abscisic acid (ABA). Fluorescence-based assays showed that systemic nitric oxide and hydrogen peroxide production was induced by 2R,3R-butanediol and correlated with expression of genes encoding nitrate reductase and nitric oxide synthase. Co-treatment of 2R,3R-butanediol with an inhibitor of nitrate reductase or an inhibitor of nitric oxide synthase lowered nitric oxide production and lessened induced drought tolerance. Increases in hydrogen peroxide were negated by co-treatment of 2R,3R-butanediol with inhibitors of NADPH oxidase, or peroxidase. These findings support the volatile 2R,3R-butanediol synthesized by certain rhizobacteria is an active player in induction of drought tolerance through mechanisms involving nitric oxide and hydrogen peroxide production.