• Title, Summary, Keyword: streptozotocin

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Drect Interaction of Streptozotocin with TBA (thiobarbituric acid) in Lipid Peroxidation Analysis (Streptozotocin에 의한 lipid peroxidation 측정시 TBA법의 적합성에 관한 연구)

  • 정진호;호지숙;문창규
    • Journal of Food Hygiene and Safety
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    • v.5 no.4
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    • pp.237-242
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    • 1990
  • This study was conducted to evaluate the usage of TBA method for the analysis of lipid peroxidation induced by streptozotocin. 5 mM streptozotocin and 1 % TBA alone showed the maximum peak at 309 nm and 358 nm respectively, although no peak was observed at 532 nm which was the wavelength to determine the absorbance for TBA method. When 5 mM streptozotocin was mixed together with 1 % TBA in vitro, new peaks at 439 nm and 532 nm had been detected, suggesting TBA did interact directly with streptozotocin forming new colored products. These results suggest that TBA method is not adequate for determination of lipid lperoxidation induced by streptozotocin.

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Streptozotocin에 의한 랫드 적혈구 손상에 관한 연구

  • 호지숙;문창규;정진호
    • Toxicological Research
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    • v.8 no.1
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    • pp.109-118
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    • 1992
  • Streptozotocin (STZ) is a naturally occurring nitrosoamide used extensively to produce diabetes in experimental animals. Since streptozotocin reportedly decrease deformability of red blood cells (RBC), we sought to investigate its potential toxicity at RBC. In addition to elevation blood glucose, 100mg/kg iv streptozotocin caused significant RBC hemolysis in female Sprague-Dawley at 48 hrs post treatment. Streptozotocin induced hemolysis was found to be dose and time-dependent` complete hemolysis required a relatively high streptozotocin concentration (500 mM, 4 hrs incubation), which is much more than in vivo dose.

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Effect of Ginseng Butanol Fraction on the Streptozotocin-induced Hyperglycemic Rats (인삼 Butanol Fraction이 Streptozotocin 유발 고혈당 쥐에 미치는 작용에 관한 연구)

  • 허인회;김대영
    • YAKHAK HOEJI
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    • v.27 no.3
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    • pp.215-220
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    • 1983
  • To elucidate the effect of ginseng butanol fraction on streptozotocin-induced hyperglycemia, ginseng butanol fraction was administered before and after injection of streptozotocin(50mg/kg, i.v.), and glucose, insulin, and cholesterol levels in serum were determined at 96 hours after streptozotocin injection. Serum glucose, insulin levels were significantly decreased by administration of ginseng butanol fraction (100mg/kg, p.o.) at 7 hour and 7, 4, 1, hour(three times) before streptozotocin injection. The glucose levels were significantly decresed by administration of ginseng butanol fraction at 1 hour (100mg/kg) after strcptozotocin injection, and also serum glucose levels in groups of continuous administration of ginseng butanal fraction(100mg/kg) for 3 days after streptozotocin injection were markedly decreased than in group of single dose of ginseng butanol fraction. Ginseng butanol fraction has the protective and relieving action against streptozotocin-induced hyperglycemia.

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Streptozotocin의 췌장독성을 유발하는 활성 본체

  • 정진호;김부영;김미정;이주영
    • Proceedings of the Korean Society of Applied Pharmacology
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    • pp.175-175
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    • 1993
  • Streptozotocin, which is a naturally occurring nitrosoamide used extensively to produce diabetes mellitus in experimental animals, has been known to be chemically stable only under acidic condition (pH 4). The present study was conducted to determine whether its degradation products formed under various conditions can induce hyperglycemia in female rats. Streptozotocin in phosphate buffer saline (pH 7.4) resulted in spontaneous degradation rapidly. Furthermore, streptozotocin incubated with plasma isolated from rats was degraded even faster than those in neutral buffer. When streptozotocin was administered iv to rats, significant elevations in blood glucose level were observed within 24 hours. However, rats treated with equimolar concentration of streptozotocin degradation products under the phosphate buffer saline as well as the plasma did not lead to hyperglycemia. These results suggest that, when streptozotocin administered undergo spontaneous breakdown in vivo, its degradation Products do not induce the hyperglycemia in rats.

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Hypoglycemic Effects of Extract Mixture of Red Ginseng and Steamed RehManiae Radix on Streptozotocin-induced Diabetic Rats (홍삼 및 숙지황의 혼합추출물의 고혈당강하작용)

  • 김학성;성연희
    • Journal of Ginseng Research
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    • v.21 no.3
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    • pp.169-173
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    • 1997
  • The effects of extract mixture of red ginseng and steamed Rehmaniae radix prepared for antidiabetic activity was examined in streptozotocin-Induced diabetic rats. The increased blood glucosec level in the streptozotocin-induced diabetic rats was significantly decreased by the treatment with the mixture (800, 1600 mg/kg, p.o.). However, neither red ginseng extract nor steamed Rehmaniae radix extract alone showed significant hypoglycemic effects. The mixture prevented a weight loss in streptozotocin-induced diabetic rats. These results suggest that the mixture has the relieving action against streptozotocin-induced hyperglycemia.

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In vitro 계에서의 Streptozotocin 분해 및 세포내 흡수

  • 박기숙;호지숙;정진호
    • Toxicological Research
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    • v.7 no.2
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    • pp.183-189
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    • 1991
  • Since streptozotocin has been known to be chemically stable only under acidic condition(pH 4), the spontaneous degradation and cellular uptake of streprozotocin in neutral incubation medium was determined by chemical assay. Streptozotocin levels in both phosphate buffered saline (pH 7.4) and Krebs-Henseleit buffer (pH 7.4) decreased with a half life of about 2 hours. The presence of erythrocytes or pepatocytes under the same buffers did not affect the streptozotocin degradation rate at all. However, streptozotocin levels in plasma isolated from rats decreased rapidly compared to those in neutral buffers.

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The Effect of Low-Intensity Treadmill Exercise on Blood Levels of Glucose, Insulin and Lipids in Streptozotocin-Induced Diabetic Rats (저강도 treadmill 운동이 streptozotocin 유도 당뇨쥐의 혈당, 인슐린 및 지질 농도에 미치는 영향)

  • 김세종;서혜림;고정림;염종우;예정복;이선주;김경환;손원협;장은숙
    • Journal of Life Science
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    • v.14 no.2
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    • pp.245-251
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    • 2004
  • Exercise is beneficial to the diabetic patients and streptozotocin-induced diabetic rat has been used for the study of exercise effect. The purpose of this study was to establish the optimal condition of induction of hyperglycemic diabetic rat using streptozotocin and to examine the preventive effect of treadmill exercise on the diabetic rat before and after streptozotocin injection. Intraperitoneal injection of increasing amount of streptozotocin up to 40 mg/kg dose-responsively induce hyperglycemic diabetic rat and inversely reduced the blood insulin level. Body weight was also gradually reduced with the increasing amount of streptozotocin. Control and diabetic rats exercised for 4 weeks before streptozotocin injection. The exercise was performed in the treadmill for 25 minutes a day and 5 times a week with low intensity (0 degree tilt, 15 m/min velocity). Following streptozotocin injection, the blood glucose level was measured every week and the rat was sacrificed after 4 weeks to measure the concentration of insulin and blood lipids. The blood levels of glucose and insulin was significantly reduced with exercise before streptozotocin injection, while those were not changed after streptozotocin injection. The levels of blood lipids such as total cholesterol, HDL-cholesterol, LDL-cholesterol and triglyceride were close to normal control rats. From this study, researchers found the optimal condition of preparation of streptozotocin-induced hyperglycemic diabetic rat, and the mild treadmill exercise has beneficial effect on preventing hyperglycemia and hyperlipidemia. Thus, even low intensive running prevent not only diabetes but also diabetic vascular complications.

Histological changes on the renal glomeruli by the Streptozotocin and Alloxan-induced diabetic mouse (Streptozotocin과 Alloxan으로 유도된 당뇨가 Mouse 신사구체에 미치는 조직학적 변화)

  • Na, Hyun-Ju
    • Journal of Korean Biological Nursing Science
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    • v.2 no.1
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    • pp.20-33
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    • 2000
  • The author was used for the ICR mouse and induced diabetes with the streptozotocin(50mg/kg)and alloxan(40mg/kg). After the testing and the identifying the diabetes, the histological changes of the glomerulus, blood test for the values of blood sugar, and urine test for the values of urine protein were investigated. The results are as follows : 1. The values of high blood sugar appeared from the 2 group were about $378mg/d{\ell}{\sim}709mg/d{\ell}$, in the treated groups with the streptozotocin and alloxan. The glycosuria were obviously continued from the 2 weeks to the 12 weeks of the streptozotocin and the alloxan treated groups and the proteinuria was ${\pm}{\sim}+$ in the 4 weeks and 8 weeks of streptozotocin treated group and were + in all the 12 weeks. The ketonuria were generally negative. 2. In the view of the light microscope, there was no significant histological changes until the 8 weeks. However, in the 12 weeks group treated with the streptozotocin, the mesangial matrix of glomerulus increased Bowman's capsules adhered to each other and changed them to the crescence shapes because of increasing the exothelial cells.

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Effects of KIOM-79 on streptozotocin-induced insulin-dependent diabetes in Sprague-Dawley rats (Streptozotocin을 이용한 제1형 당뇨 유발 흰쥐에서 KIOM-79의 효과)

  • Lee, Jong Hyuk;Yoon, Sang-Pil;Kim, Jin-Sook;Chang, In-Youb
    • The Journal of Medicine and Life Science
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    • v.15 no.2
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    • pp.72-78
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    • 2018
  • We investigated the effect of KIOM-79, 80% ethanolic extract of herbal prescription isolated from Magnolia officinalis, Pueraria lobate, Glycyrrhiza uralensis, and Euphorbia pekinensis, on streptozotocin-induced diabetes in Sprague-Dawley rats. The rats were treated orally with KIOM-79 (500 mg/kg/day) 1) for 3 days prior to streptozotocin (60 mg/kg, intraperitoneal) injection or 2) for 9 weeks after establishing diabetes model to examine acute and chronic effects on hyperglycemia and biochemical variables, respectively. As a result, KIOM-79 had little effects on hyperglycemic changes in acute model. Sexual comparison, however, showed reduced hyperglycemia in female rats, especially 24 hours after streptozotocin injection with or without KIOM-79 pretreatment. In chronic model, streptozotocin-induced hyperglycemia was well established, but KIOM-79 treatment showed no statistically significant effects on all variables. Thus, based on our findings KIOM-79 might have little effects on streptozotocin-induced insulin-dependent diabetes although it has been known to have hypoglycemic and antidiabetic effects on non-insulin-dependent diabetes models.

Effects of Hwanggeum-tang Aqueous Extracts on Streptozotocin-induced Rat's Diabetes and Related Complications (황금탕이 Streptozotocin으로 유발된 당뇨병 백서의 혈당 및 당뇨병합병증에 미치는 영향)

  • Choi, Byeong-Heon;Yoon, Gyeong-Min;Kang, Seok-Bong
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.4
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    • pp.683-690
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    • 2011
  • The object of this study was to observe the effects of Hwanggeum-tang (HGT) aqueous extracts on Streptozotocin-induced rat's diabetes and related complications. Three different dosages of HGT extracts were orally administered oncea day for 28 days from 3 weeks after Streptozotocin treatment (60 mg/kg, single intraperitoneally administered). All the rats were checked at 3 weeks after Streptozotocin treatment as follows. Changes on the body weight, blood glucose level, kidney weight, serum BUN and creatinine level, liver weight, serum AST and ALT level, serum LDL, HDL, triglyceride and total cholesterol level were observed with changes on the pancreatic MDA content and GSH content. The results were compared with a potent antioxidant silymarin 100 mg/kg in which the effects on Streptozotocin-induced diabetes and related complications were already confirmed. As results of Streptozotocin-injected diabetes and related complications, dramatical decreases on the body weight, increase of the kidney and liver weight, increase of serum BUN, creatinine, AST, ALT, LDL, triglyceride, total cholesterol level and decreases of serum HDL level were detected in streptozotocin control as compared with intact control. In addition, marked increases of pancreatic MDA content and decreases of GSH content were also detected in streptozotocin control as compared with intact control. However, these diabetes and related complications, and inhibition of antioxidant effects induced by Streptozotocin were inhibited by 28 days continuous treatment of 50, 100 and 200 mg/kg of HGT extracts in the present study. HGT have favorable effects on the diabetes and various diabetic complications. Therefore, more detail mechanism studies should be conducedin future with the efficacy tests of individual herbal composition of HGT and the screening of the biological active compounds in herbs.