• 한국식품위생안전성학회지
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• 제5권4호
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• pp.237-242
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• 1990

Streptozotocin에 의한 적혈구의 막 lipid peroxidation 측정시 TBA법의 적합성에 관하여 검토하기 위하여 Streptozotocin와 TBA의 직접반응 여부를 연구하였다. 여러 농도의 Streptozotocin 와 20% RBC suspension을 함께 배양한 후 lipid peroxidation을 TBA법으로 측정했을 때, Streptozotocin의 농도에 따라 흡광도가 증가하다가 100mM부터는 plateau를 이루었다. 그런데 적혈구를 제거한 대조실험에서도 발색단이 관찰되었다. 시험관에서 5mM의 Streptozotocin와 1% TBA를 산성조건에서 가열 직접 반응시킨 후 spectrum을 찍은 결과 TBA법 측정파장인 532nm에서 새로운 peak가 생겼다. 또 이 파장에서의 흡광도는 Streptozotocin 농도에 따라 증가하다가 25mM부터는 plateau가 되었다. 이러한 결과들은 적혈구와 Streptozotocin를 함께 배양하여 lipid peroxidation을 측정할 때, cell 내로 유입된 Streptozotocin 가 TBA와 직접반응을 할 수 있음을 제시한다. 그리고 다른 측정법인 lipid conjugation은 233nm에서의 흡수를 측정하는데. Streptozotocin 자체가 200-300nm에서 흡수를 나타내므로 이 방법으로도 lipid peroxidation 측정을 방해할 수 있다. 따라서 Streptozotocin에 의한 적혈구막의 lipid peroxidation은 TBA method 및 lipid conjugation 방법으로 측정하기는 부적합한 것으로 사료된다.

• Toxicological Research
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• 제8권1호
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• pp.109-118
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• 1992

Streptozotocin (STZ) is a naturally occurring nitrosoamide used extensively to produce diabetes in experimental animals. Since streptozotocin reportedly decrease deformability of red blood cells (RBC), we sought to investigate its potential toxicity at RBC. In addition to elevation blood glucose, 100mg/kg iv streptozotocin caused significant RBC hemolysis in female Sprague-Dawley at 48 hrs post treatment. Streptozotocin induced hemolysis was found to be dose and time-dependent` complete hemolysis required a relatively high streptozotocin concentration (500 mM, 4 hrs incubation), which is much more than in vivo dose.

• 약학회지
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• 제27권3호
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• pp.215-220
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• 1983

To elucidate the effect of ginseng butanol fraction on streptozotocin-induced hyperglycemia, ginseng butanol fraction was administered before and after injection of streptozotocin(50mg/kg, i.v.), and glucose, insulin, and cholesterol levels in serum were determined at 96 hours after streptozotocin injection. Serum glucose, insulin levels were significantly decreased by administration of ginseng butanol fraction (100mg/kg, p.o.) at 7 hour and 7, 4, 1, hour(three times) before streptozotocin injection. The glucose levels were significantly decresed by administration of ginseng butanol fraction at 1 hour (100mg/kg) after strcptozotocin injection, and also serum glucose levels in groups of continuous administration of ginseng butanal fraction(100mg/kg) for 3 days after streptozotocin injection were markedly decreased than in group of single dose of ginseng butanol fraction. Ginseng butanol fraction has the protective and relieving action against streptozotocin-induced hyperglycemia.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.175-175
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• 1993

Streptozotocin, which is a naturally occurring nitrosoamide used extensively to produce diabetes mellitus in experimental animals, has been known to be chemically stable only under acidic condition (pH 4). The present study was conducted to determine whether its degradation products formed under various conditions can induce hyperglycemia in female rats. Streptozotocin in phosphate buffer saline (pH 7.4) resulted in spontaneous degradation rapidly. Furthermore, streptozotocin incubated with plasma isolated from rats was degraded even faster than those in neutral buffer. When streptozotocin was administered iv to rats, significant elevations in blood glucose level were observed within 24 hours. However, rats treated with equimolar concentration of streptozotocin degradation products under the phosphate buffer saline as well as the plasma did not lead to hyperglycemia. These results suggest that, when streptozotocin administered undergo spontaneous breakdown in vivo, its degradation Products do not induce the hyperglycemia in rats.

• 한방안이비인후피부과학회지
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• 제20권1호통권32호
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• pp.51-65
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• 2007

Objective : The present study was carried out to investigate the preventive effect of Sophorae Flos on streptozotocin - induced Diabetes mellitus. Method : Sophorae Flos was given to rats with oral administration. The experimental animals were divided into 3groups : normal group of rats, control group of Streptozotocin-induced diabetic rats, sample group with Sophorae Flos . The effect of Sophorae Flos on Streptozotocin-induced diabetes was observed by measuring the survival rate of rats, weight of rats, FER, blood glucose, each organ weight of rat, total cholesterol, HDL, GOT, GPT & creatinine. Result : Streptozotocin caused hyperglycemia and hypoinsulinemia by a selectively destroying pancreatic ${\beta}-cell$. Sophorae Flos treatment don't protected them from hyperglycemia and hypoinsulinemia. Organ weight liver, kidney, heart & spleen shows no significant changes. Sophorae Flos significantly don't recoverd the increase of several biochemical parameters such as blood glucose, total cholesterol, HDL, GOT, GPT, antioxidant & creatinine is vice versa. Conclusion : Sophorae Flos extract group did not show significant decrease than Streptozotocin control group.

• Journal of Ginseng Research
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• 제21권3호
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• pp.169-173
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• 1997

The effects of extract mixture of red ginseng and steamed Rehmaniae radix prepared for antidiabetic activity was examined in streptozotocin-Induced diabetic rats. The increased blood glucosec level in the streptozotocin-induced diabetic rats was significantly decreased by the treatment with the mixture (800, 1600 mg/kg, p.o.). However, neither red ginseng extract nor steamed Rehmaniae radix extract alone showed significant hypoglycemic effects. The mixture prevented a weight loss in streptozotocin-induced diabetic rats. These results suggest that the mixture has the relieving action against streptozotocin-induced hyperglycemia.

• Toxicological Research
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• 제7권2호
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• pp.183-189
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• 1991

Since streptozotocin has been known to be chemically stable only under acidic condition(pH 4), the spontaneous degradation and cellular uptake of streprozotocin in neutral incubation medium was determined by chemical assay. Streptozotocin levels in both phosphate buffered saline (pH 7.4) and Krebs-Henseleit buffer (pH 7.4) decreased with a half life of about 2 hours. The presence of erythrocytes or pepatocytes under the same buffers did not affect the streptozotocin degradation rate at all. However, streptozotocin levels in plasma isolated from rats decreased rapidly compared to those in neutral buffers.

• 생명과학회지
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• 제14권2호
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• pp.245-251
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• 2004

본 연구는 streptozotocin으로 당뇨병을 유도하는 조건들을 농도에 따른 변화를 관찰하고, 적절한 조건에서 당뇨쥐에서의 저강도의 treadmill 운동이 체중과 혈중의 당, 인슐린 및 지질의 변화에 미치는 영향을 알아보고자 실시하였으며, 연구결과를 요약하면 다음과 같다. 실험 I에서 STZ 주사량이 40 mg/mg으로 높아질수록 정상군에 비해 혈당이 유의하게 증가하고, 반대로 체중과 인슐린 농도는 상대적으로 감소하였다. 실험II에서 STZ 주사를 한 모든 군에서 혈당이 유의하게 증가하지만 BE군은 C군에 비해 그 증가율이 유의하게 억제되었으며, 운동이 인슐린 농도와 혈중지질 농도를 정상적으로 유도하는 것으로 나타났다. 특히, 당뇨 유발 전부터 운동을 실시한 경우 그 효과가 더욱 유의하게 나타났으며, 앞으로 당뇨 유발의 정도와 운동 빈도 및 강도와 관련된 더욱 많은 연구가 이루어져야 할 것으로 사료된다.

• Journal of Korean Biological Nursing Science
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• 제2권1호
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• pp.20-33
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• 2000

The author was used for the ICR mouse and induced diabetes with the streptozotocin(50mg/kg)and alloxan(40mg/kg). After the testing and the identifying the diabetes, the histological changes of the glomerulus, blood test for the values of blood sugar, and urine test for the values of urine protein were investigated. The results are as follows : 1. The values of high blood sugar appeared from the 2 group were about $378mg/d{\ell}{\sim}709mg/d{\ell}$, in the treated groups with the streptozotocin and alloxan. The glycosuria were obviously continued from the 2 weeks to the 12 weeks of the streptozotocin and the alloxan treated groups and the proteinuria was ${\pm}{\sim}+$ in the 4 weeks and 8 weeks of streptozotocin treated group and were + in all the 12 weeks. The ketonuria were generally negative. 2. In the view of the light microscope, there was no significant histological changes until the 8 weeks. However, in the 12 weeks group treated with the streptozotocin, the mesangial matrix of glomerulus increased Bowman's capsules adhered to each other and changed them to the crescence shapes because of increasing the exothelial cells.

• Journal of Medicine and Life Science
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• 제15권2호
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• pp.72-78
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• 2018

We investigated the effect of KIOM-79, 80% ethanolic extract of herbal prescription isolated from Magnolia officinalis, Pueraria lobate, Glycyrrhiza uralensis, and Euphorbia pekinensis, on streptozotocin-induced diabetes in Sprague-Dawley rats. The rats were treated orally with KIOM-79 (500 mg/kg/day) 1) for 3 days prior to streptozotocin (60 mg/kg, intraperitoneal) injection or 2) for 9 weeks after establishing diabetes model to examine acute and chronic effects on hyperglycemia and biochemical variables, respectively. As a result, KIOM-79 had little effects on hyperglycemic changes in acute model. Sexual comparison, however, showed reduced hyperglycemia in female rats, especially 24 hours after streptozotocin injection with or without KIOM-79 pretreatment. In chronic model, streptozotocin-induced hyperglycemia was well established, but KIOM-79 treatment showed no statistically significant effects on all variables. Thus, based on our findings KIOM-79 might have little effects on streptozotocin-induced insulin-dependent diabetes although it has been known to have hypoglycemic and antidiabetic effects on non-insulin-dependent diabetes models.