• Korean Journal of Microbiology
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• v.36 no.2
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• pp.109-111
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• 2000

A 3.5 kb plasmid from Lactobacillus. casei ssp. cosei NCIB 4114 was isolated and E. cali-L. casei shuttle vectors were constructed containing this plasmid. Transformation by electroporation was successful with all the plasmids constructed. Optimized condition for the electroporation was established with efficiency level of $2{\times}10^5$ transformants per $\mu$g of vector DNA. Successful introduction of those shuttle vectors enable to these vectors as food grade vector for lactic acid bacteria.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2001.10a
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• pp.579-582
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• 2001

Vector Transformation is a new method unified vector quantization and coding. So far, codebook generation applied to coding was LBG algorithm. But using the advantage of SOFM(Self-Organizing Feature Map) based on neural network can improve a system's performance. In this paper, we generated VTC(Vector Transformation Coding) codebook applied with SOFM algorithm and compare the result for several coding rates with LBG algorithm. The problem of Vector quantization is complicated calculation and codebook generation. So, to solve this problem, we used neural network approach method.

• Korean Journal Plant Pathology
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• v.11 no.1
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• pp.66-72
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• 1995

The coat protein (CP) gene of cucumber mosaic virus-As (CMV-As) strain was engineered for expression in the plant by using the cauliflower mosaic virus 35S transcript regulatory sequences. The CP gene was cloned into an Agrobacterium-derived binary vector. A chimeric gene was constructed by the cDNA of CMV-As CP and plant expression vector pBI121. The clone, pCMAS66, was first introduced into the phagemid vector pSPORT1 for situating sense orientation for translation and making restriction sites in order to re-introduce plant expression vector, pHI121. The resulting subclone pCASCP02 and plant expression vector pBI121 were treated with BamHI-SacI for excising the target gene and removing GUS gene, respectively. After Agrobacterium transformation by freeze-thaw technique, the clone, pCMASCP121-123 which contains sense orientation of the target gene, was selected and confirmed by restriction endonuclease analysis. The CMV-As CP gene was introduced into A. tumefaciens. The results on tobacco plant transformation with the vector system revealed that the system could be successfully introduced and showed high frequency of selection to putative transformations.

• Proceedings of the KIEE Conference
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• 1997.07b
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• pp.422-424
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• 1997

In this paper, we characterize the whole class of vector fields that can be linearized by a given nominal state transformation and a feedback linearizing controller. The necessary and sufficient condition for a given uncertain vector field to be so-called "completely linearizable by the nominal coordinate transformation" is given in terms of Lie Bracket of uncertain vector fields and some suitable vector fields of the nominal system.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.323-330
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• 2019

The high expression level of industrial and metabolically important proteins in plants can be achieved by plastid transformation. The CaIA vector, a Capsicum-specific vector harboring aadA (spectinomycin resistance), is a selectable marker controlled by the PsbA promoter, and the terminator is flanked by the trnA and trnI regions of the inverted repeat (IR) region of the plastid. The CaIA vector can introduce foreign genes into the IR region of the plastid genome. The biolistic method was used for chloroplast transformation in Scoparia dulcis with leaf explants followed by antibiotic selection on regeneration medium. Transplastomes were successfully screened, and the transformation efficiency of 3 transgenic lines from 25 bombarded leaf explants was determined. Transplastomic lines were evaluated by PCR and Southern blotting for the confirmation of aadA insertion and its integration into the chloroplast genome. Seeds collected from transplastomes were analyzed on spectinomycin medium with wild types to determine genetic stability. The increased chloroplast transformation efficiency (3 transplastomic lines from 25 bombarded explants) would be useful for expressing therapeutically and industrially important genes in Scoparia dulcis L.

• The Plant Pathology Journal
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• v.20 no.2
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• pp.158-163
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• 2004

Chlorella is a eukaryotic microalgae which shares metabolic pathways with higher plants. These charac-teristics make chlorella a potential candidate for eukaryotic overexpression systems. Recently, a foreign flounder growth hormone gene was stably introduced and expressed in transformed Chlorella ellipsoidea by using a modified plant transformation vector that contains cauliflower mosaic virus (CaMV) 35S pro-moter and the phleomycin resistant Sh ble gene as a selection marker. In this study, this same vector was modified by incorporating a promoter and a 3' UTR region of the 33kDa peptide gene from a chlorella virus that was isolated in our laboratory. The 33kDa gene promoter was used to replace the 35S promoter and the 3' UTR was introduced to separate the target gene and downstream Sh ble gene. Three different chlorella transformation vectors containing human erythropoietin (EPO) gene were constructed. The mp335EPO vector consists of a promoter from the 33kDa peptide gene, whereas the mp3353EPO vector contains the same promoter from the 33kDa peptide gene and its 3' UTR. The mp35S33pEPO vector contains the 35S promoter and the 3' UTR from the 33 kDa peptide gene. There was no significant difference in the expression levels of EPO protein in chlorella cells transformed with either of three of the transformation vectors. These data indicate that the promoters from the chlorella virus are comparable to the most common CaMV 35S promoter. Furthermore, these data suggest that other promoters from this virus can be used in future construction of chlorella transformation system for higher expression of target proteins.

• Korean Journal of Microbiology
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• v.27 no.2
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• pp.92-97
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• 1989

the vector, pGUR19, for Rhizobium gene manipulation, was constructed by combining the replication and mobilization function of indigenous multicopy plasmid from Acacia(Robinia pseudoacacia L.) Rhizobia sp86 with E. coli cloning vehicle, pBR322. The vector could be efficiently mobilized by RP4 tra function incorporated into chromosome of E. coli named SM10 and efficiently transferred to various gram negative hosts including Rhizobium and Afrobacterium by transformation. Mobilization frequency of the constructed vector was ranged from $1.2\times 10^{-2}$ (E.coli HB 101) to $4.6\times 10^{-4}$ (A. tumefaciens 15955) and transformation frequency was ranged from $5.4\times 10^{-7}$(E. coli HB101) to $1.2\times 10^{-10}$ (A. tumefaciens 15955). The vector, pGUR19, was stably replicated and maintained in a variety of Rhizobium and Agrobacterium.

• Journal of international Conference on Electrical Machines and Systems
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• v.3 no.2
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• pp.116-120
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• 2014

3-phase Induction machines are being used in industry and dq transformation from 3 phase of a, b, c is commonly used to analyze these machines. The equivalent circuits of d and q axis are however generally cross coupled and not simple to analyze. In this study, an analysis method of 3ph induction motor considering current regulator using dq transformation and matrix vector is proposed and it can explain the 3ph induction motor physically. This model does not need the separating process of d and q components. With this technique, the model becomes simple, is easy to understand in physical, and can get the same results with those from the other dq models. These simulation results of the proposed model are compared with those of other models for the conformation of the proposed method.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.11
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• pp.1601-1605
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• 2012

Three-phase voltage and current is applied to the three-phase alternating current motors which are commonly used in industry. Three phase variables of a, b, c are converted into d, q, 0 axis and the AC machines are modeled and analyzed. Basically the coordinate transformation or d-q transformation is used for convenience, a few steps are needed to analyze the motor performances - separating d and q components, establishing each equivalent circuit, and solving the differential equations of the circuits. In this study, a modeling technique of induction motor using complex vector is proposed and it can explain the induction motor physically. This method does not need the separating process of d and q components. With this technique, the model becomes simple, is easy to understand in physical, and can get the same results with those from the other models. These simulation results of the proposed model are compared with them for the conformation of the proposed method.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.27 no.8
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• pp.98-104
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• 2013

AC machines are in wide use in industry and d-q transformation from 3 phase of a, b, c is commonly used to analyze these kinds of machines. The equivalent circuits of d and q axis are, however, generally cross coupled and difficult to analyze. In this study, a modeling technique of AC machine including induction and PM synchronous motors using matrix vector is proposed. With that model, it can not only explain the AC machines physically but also make it simple to analyze them. The separating process of d and q components is not needed in this model and this model can be applied to analyze asymmetric motors like IPMSM machine. With this technique, the model becomes simple, easy to understand physically, and yields results that are the same as those from other models. These simulation results of the proposed model for induction motor are compared with those of other models to verify the method proposed.