• Title/Summary/Keyword: trisodium phosphate

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Discovery of Parasite Eggs in Archeological Residence during the 15th Century in Seoul, Korea

  • Cho, Pyo Yeon;Park, Jung-Min;Hwang, Myeong-Ki;Park, Seo Hye;Park, Yun-Kyu;Jeon, Bo-Young;Kim, Tong-Soo;Lee, Hyeong-Woo
    • The Korean Journal of Parasitology
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    • v.55 no.3
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    • pp.357-361
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    • 2017
  • During civil engineering construction near Sejong-ro, Jongro-ku, Seoul, cultural sites were found that are thought to have been built in the 15th century. This area was home to many different people as well as the leaders of the Yi dynasty. To gain further insight into the life styles of the inhabitants of the old capital, soil samples were collected from various areas such as toilets, water foundations, and drainage ways. Parasite eggs were examined by microscopy after 5 g soil samples were rehydrated in 0.5% trisodium phosphate solution. A total of 662 parasite eggs from 7 species were found. Species with the highest number of eggs found were Ascaris lumbricoides (n=483), followed by Trichuris trichiura (138), Trichuris vulpis (21), Fasciola hepatica (8), Clonorchis sinensis (6), Paragonimus westermani (4), and Metagonimus yokogawai (2). These findings indirectly indicate the food habits of the people in Yi dynasty.

Studies on Salmonella enteritidis Contamination in Chicken Egg using Confocal Scanning Laser Microscopy (Confocal Scanning Laser Microscopy 를 이용한 계란에서의 Salmonella enteritidis 오염 연구)

  • Jang, Keum-Il;Park, Jong-Hyun;Kim, Kwang-Yup
    • Korean Journal of Food Science and Technology
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    • v.31 no.3
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    • pp.771-777
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    • 1999
  • The structural function of three egg membrane layers and cuticle layer, and the effectiveness of 5 film coatings (chitosan, starch, gelatin, dextrin, mineral oil) on the prevention of Salmonella enteritidis penetration was investigated using confocal scanning laser microscopy (CSLM). Diameters of outer membrane fibers, inner membrane fibers and limiting membrane particles in eggshell were $1.5{\sim}7.2$, $0.8{\sim}2.0$ and $0.1{\sim}1.4\;{\mu}m$, respectively and average thicknesses were 10.0, 3.5, $3.6\;{\mu}m$, respectively. Average thickness of cuticle layer was $6.0\;{\mu}m$ and cuticle layer covered $40{\sim}80%$ of total eggshell surface. Average coating films thickness for chitosan, starch, gelatin, dextrin and mineral oil were 2.2, 2.5, 3.9, 3.6 and $5.0\;{\mu}m$, respectively. After immersion process eggshell surface was almost completely covered by coating films. Chitosan coating was most effective among 5 film coatings in inhibiting growth of Salmonella enteritidis. Penetration process of Salmonella enteritidis through eggshell was investigated by multicolor imaging using CSLM and plate counting. Cuticle layer was the most important structure in blocking the penetration. Among 5 film coatings, chitosan showed the best and similar effectiveness with cuticle layer.

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