• Journal of Nutrition and Health
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• v.27 no.2
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• pp.153-161
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• 1994

We fed high trypotophan diet(3.5% tryptophan/diet(w/w) to mice for 7 days and treated then with 3 hour immobilization(IMMB) stress to investigate tryptophan metabolism and immunomodulation. The levels of serum tryptophan, brain tryptophan, serotonin(5HT) and 5-hydroxyindoleacetic acid(5HIAA) in the tryptophan diet fed animals were higher than those of the normal diet fed animals. Feeding tryptophan supplemented diet to stressed animal significantly decreased the levels of serum and brain tryptophan and 5HT levels. However, the amount of 5HIAA which is the metabolite of serotonin was increased in brain. Plasma corticosterone level was increased by the stress in both groups but the degree of this increase was smaller in high tryptophan fed animals. The relative numbers of CD8+ T cells, CD4+ T cells and B cells in spleen were decreased in high tryptophan diet fed and stressed animals compared to control diet fed and no stressed animals. CD8+ T cells decreased more than CD4+ T cells. The decrease of CD8+ T cells in high tryptophan fed and stressed animals was similar to that in high tryptophan fed animals or normal diet fed and stressed animals. Stress and tryptophan supplement acted synergistically to decrease the number of B cells. This study suggests that stress and tryptophan supplement could modify the number of lymphocyte cells, and indicates that the interaction of stress and tryptophan supplement on immune fuction depends on the types of immune cells.

• Journal of Nutrition and Health
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• v.26 no.3
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• pp.231-247
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• 1993

This study was designed to confirm the effect of dietary protein level and oral administration of tryptophan on brain serotonin metabolism. Two animal experiments were conducted. The objectives and results of research were as follows : In the first experiment, it was investigated whether administration of reserpine to Sprague-Dawley rats fed 6% or 20% casein diet induced decrease in serum tryptophan and large neutral amino acid(LNAA) concentrations, tryptophan/LNAA concentration ratio, brain tryptophan, serotonin and 5-hydroxyindoleacetic acid(5-HIAA) contents. Brain serotonin content of 6% casein diet group was lower than those of 20% casein diet group. Both 6% and 20% casein diet groups administered with reserpine to induce the analogous depression, showed the notable decrease in brain serotonin content when they were compared with 20% casein diet group not administered with reserpine. Serum tryptophan/LNAA ration and brain 5-HIAA content showed a tendency similar to the change of serotonin content, but the mean difference among all groups was not significant. From these results, it could be said that when the dietary protein level was low, brain serotonin content was decrease. The second experimnt was to see the change in serum tryptophan concentration and tryptophan/LNAA ratio and brain tryptophan, serotonin and 5-HIAA content when tryptophan was administered orally to the animals treated with reserpine. Serum tryptophan concentration tended to increase in both reserpine-treated 6% and 20% casein diet groups administered with tryptophan, especially in the 6% casein diet group. Serum tryptophan/LNAA concentration ratio tended to incrase in reserpine-tteated 6% casein diet group, while decrease in reserpine-treated 20% casein diet group. Brain tryptophan content was increased in both reserpine-treated 6% and 20% casein diet groups. However, brain serotonin content of reserpine-treated 6% casein diet group showed a tendency to decrease, while that of reserpine-treated 20% casein group increase. Consequently, the effect of tryptophan administration on increase of brain tryptophan and serotonin content in animals treated with reserpine was far more excellent in 20% casein diet groups. It was concluded that dietary protein intake and tryptophan administration increase brain serotonin level. Accordingly, it was possible to confirm that brain function, particularly in aspect of behavior related to the serotonin, was changed with manipulation of dietary composition.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.1
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• pp.75-79
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• 2005

There were three trials involved in this experiment. All piglets in Trial 1 were randomly distributed into the following 4 treatments. Treatment 1. Corn-soybean diet with 5% SDPP. The tryptophan level was 0.237%. Treatment 2. Corn-soybean diet with 10% meat and bone meal. The tryptophan level was 0.177%. Treatment 3. Treatment 1+0.0662% synthetic tryptophan. The total tryptophan level was 0.303. Treatment 4. Treatment 2+0.0662% synthetic tryptophan. The total tryptophan level was 0.236. Piglets in Trial 2 were distributed randomly into the following 4 treatments. Treatment 1: corn-soybean diet+10% meat and bone meal. The total tryptophan level was 0.176%. Treatment 2: corn-soybean diet+10% meat and bone meal+5% SDPP. The total tryptophan level was 0.180%. Treatment 3: Treatment 1 diet+0.004% synthetic tryptophan. The total tryptophan level was 0.180%. Treatment 4: Treatment 1 diet+0.631% synthetic tryptophan. The total tryptophan level was 0.237%. There were 4 treatments in Trial 3. Treatment 1: cornsoybean diet+10% meat and bone meal. The total tryptophan level was 0.176%. Treatment 2: Treatment 1 diet+0.061% synthetic tryptophan. The total tryptophan level was 0.237%. Treatment 3: Treatment 2 diet+0.061% synthetic tryptophan. The total tryptophan level was 0.298%. Treatment 4: corn-soybean diet+10% meat and bone meal+5% SDPP. The total tryptophan level was 0.180%. The results of Trial 1 showed that the piglets ate significantly more (p<0.05) when feed included SDPP in the diet during the first 2 weeks. The feed intake also increased when synthetic tryptophan was added in the 5% meat and bone meal diet; however, the difference did not reach a significant level (p>0.05) during the first 2 weeks. Three weeks onwards the feed intake of 5% meat and bone meal treatment was significantly lower (p<0.05) than for the other three treatments. The results of Trial 2 showed that the feed intake could be significantly improved only when the total tryptophan level reached 0.237%. Piglets in the 5% SDPP treatment had higher feed intake than piglets in 10% meat and bone meal treatment with 0.180% of tryptophan, but did not reach a significant level (p<0.05). Body weight gain also had the same trend as feed intake. The pigs in Treatment 1, the lowest total level of tryptophan treatment (0.176%), had lowest feed intake and weight gain, but the difference did not reach a significant level (p>0.05). The pigs in Treatment 1 of Trial 3 had the lowest feed intake and weight gain (p>0.05). Treatment 2 (0.237%) had the highest average feed intake from Week 1 to Week 5; the second best result was recorded in Treatment 4. As for the weight gain of the piglets in Treatment 4 (5% SDPP), they had a higher average weight during the first 3 weeks. The feed efficiency was better for Treatment 4 (5% SDPP) during the first 2 weeks. The results of these trials showed that both SDPP and tryptophan had a trend to improve the feed intake and weight gain.

• Korean Journal of Food Science and Technology
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• v.5 no.3
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• pp.174-177
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• 1973

Total and free tryptophan contents in the fore shank muscle of korean cow treated with proteolytic enzymes have been analyzed by the spectrophotometric method and the results are as follows: 1. By adding 0.01%, 0.05%, 0.1% of proteolytic enzymes, the total tryptophan and free tryptophan have incresed steadily 2. The rate of increase of the total tryptophan content was highest when 0.05% of the enzymes for the meat weight were added. 3. The change in free tryptophan incident to the addition of proteolytic enzymes was insignificant.

• YAKHAK HOEJI
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• v.35 no.4
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• pp.326-331
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• 1991

Brain serotonin and its utilization was investigated on stressed rats after feeding high tryptophan diet for a month. High tryptophan fed rats displayed significantly higher level of serum tryptophan, brain tryptophan, serotonin (5-hydroxytryptamine; 5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) than the control diet fed rats. When rats were treated with 3 hour immobilization (IMMB) stress, serotonin turnover was slightly increased, but not statistically significant, in control diet group rats. However in high tryptophan diet rats, 3 hr IMMB stress resulted in statistically significantly (p<0.05) decreased the serum tryptophan, brain tryptophan and 5-HT level. The concentration of 5-HIAA was significantly increased indicating accelerated utilization of the brain 5-HT of the high trp. fed rat. The utilization pattern of the serotonin was found to be similar among young and adult rats. Rats on a tryptophan enriched diet displayed higher coping ability to the stress as they exhibited smaller increment of corticosterone level. A possble involvement of opioid system was suggested in serotonin utilization by measuring total $^{3}$[H]-naloxone binding in brain.

• Microbiology and Biotechnology Letters
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• v.15 no.5
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• pp.334-339
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• 1987

Enzymatic conversion of pyruvic acid produced by microorganism to tryptophan was investigated. A luminescent bacteria. Beneckea sp., was used for the production of pyruvic acid. As a source of tryptophanase which synthesizes tryptophan from pyruvic acid, indole and ammonia, whole cells of Enterobacter aerogenes ATCC 10031 were used directly in the reaction mixture. To increase the production of tryptophan, nonionic detergents and nonaqeous organic solvents were used ms reserviors of indole in the reaction mixture. In the case of nonionic detergents, TritonX-100 was very effective. When 1.5% of Triton X-100 was used, 7.7g/$\ell$ of tryptophan was produced at 37$^{\circ}C$ for 48hr. In the case of nonaqueous solvents, 8.7g/$\ell$ of tryptophan was produced at 37$^{\circ}C$ for 48 hr, when 10% of benzene was used. This amount of tryptophan corresponds to conversion of 48% of Indole and 36% of pyruvic acid, respectively.

• Microbiology and Biotechnology Letters
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• v.22 no.5
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• pp.557-560
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• 1994

The optimum medium composition for the production of L-tryptophan with Klebsiella pnuemoniae pheA tyrA trpE trpR/pSC 101-trp$^{+}$ and the effect of precusors in the optimum medium were studied. The specific growth rate in the optimum medium was almost the same as that in the basal medium, the former showing 1.01 and the latter 1.07 hr $$^{-1}$, but the produced tryptophan was increased 45% in the optimum medium. The maximum amount of produced tryptophan was 159 mg/l within 14 hours. Tryptophan production was ceased by casamino acid addition over 4 g/l in medium, but cell maSS increased with its addition. Indole and anthranilate as precusors had toxic effect on growth and tryptophan production at experimented concentration range (over 20 mg/l), but L-serine had good effect on tryptophan production, resulting in 175 mg/l tryptophan within 14 hours.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.4
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• pp.647-651
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• 1992

Two experiments involving comparative slaughter procedures were conducted to see if the decrease in total energy retention (ER) resulted from the decreased food intake in growing chicks fed on a diet containing tryptophan less than the requirement. Ad libitum-feeding a diet containing 50% of tryptophan of a control diet (1.5 g/kg) decreased body weight gain, apparent metabolizable energy intake (AMEI), ER and ER : AMEI ratio. When both the control diet and the 0.75 g/kg tryptophan diet were tube-fed at the two levels of food intake, body weight gain was significantly lower in chicks on the low tryptophan diet than in the control chicks at each level of intake. AME : gross-energy ratio decreased only when the low tryptophan diet was tube-fed at the higher level of intake. Energy retained as protein was significantly decreased by the low tryptophan level and reduction of food intake. Energy retained as fat was affected by food intake. ER and ER : AMEI ratio were unaffected by dietary tryptophan level and were proportional to AMEI. Heat increment of feeding was affected by neither tryptophan nor food intake. These results indicate that the decreased ER in chicks fed on the low tryptophan diet was due mainly to the decreased food intake and not to the decreased efficiency of ME utilization.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.3
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• pp.284-288
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• 1997

Purified diets containing five graded levels of tryptophan were fed to growing chicks to evaluate tryptophan requirements for growth and maintenance. A model was developed to separate tryptophan requirement for maintenance from requirement for growth. From this model, the daily tryptophan requirement for growth was 2.16 mg/g gain, and the daily requirement for maintenance 0.029 times metabolic body size ($Wg^{0.75}$). Based on nitrogen gain response, the tryptophan requirement for growth was 0.078 mg/mg N gain, and the daily maintenance requirement was 0.029 times metabolic body size. The total tryptophan requirements were 71.56 mg/day or 0.173% of the diet, 69.48 mg/day or 0.168% of the diet based on the weight gain response and nitrogen gain response, respectively. Previous tryptophan requirements for growing chicks aging 1-28 days are in close agreement with these estimates. Based on the relationship of weight gain and N gain, about 1.25% of the retained CP was consisted of tryptophan; the previously reported value of tryptophan content of chick muscle CP was 1.03%.

• Applied Biological Chemistry
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• v.25 no.3
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• pp.155-160
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• 1982

Claviceps purpurea PRL 1980 produces a fluorescent reddish brown pigment in the alkaloid production medium. When D,L-tryptophan $[side\;chain-3-^{14}C]$ was administered into the production medium, the radioactive pigment and 5-hydroxytryphan were isolated from the cultures. Conversion of tryptophan to 5-hydroxytryptophan in vivo was shown by an isotopic trapping procedure. 5-hydroxytryptophan isolated from the cultures contained appreciable radioactivity and was recrystallized to constant specific radioactivity. The injection of the $^{14}C-labelled$ 5-hydroxytryptophan showed an incorporation of radioactivity into brown pigment significantly higher than that of tryptophan. The brown pigment produced by Claviceps purpurea PRL 1980 seems to be derived from tryptophan through 5-hyrdroxytryptophan.