• Preventive Nutrition and Food Science
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• 제5권1호
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• pp.10-14
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• 2000

A simple and rapid method was developed to prepare a large quantity of highly pure conjugated linoleic acid (CLA) chemically-synthesized from safflower seed oil (SSO). CLA-SSO(74.9% in purity) was synthesized from fresh SSO(79.9% of linoleic acid) by alkaline isomerization at 18$0^{\circ}C$. Urea(50g) and CLA-SSO (25g) were completely dissolved in ethanol (750ml) using a water bath(5$0^{\circ}C$) and followed by refluxing for 60 min. The resultant was cooled to room temperature and stored in a cold room (4$^{\circ}C$) for 24hrs. After removing the urea adduct by filtration, the filtrate was rotoevaporated under 4$0^{\circ}C$ and the residue was dissolved in hexane (200ml). The hexane extract was washed with distilled water (100ml$\times$3) and dried over sodium sulfate anhydrous. This urea treatment procedure was repeated three times. The purity of CLA recovered from the hexane extract was 95.0%. This method can be applied to prepare a large quantity of highly pure chemically-synthesized CLA (>0.5kg/a batch) from any plant oils containing high percentages (>70%) of linoleic acid.

• 한국식품과학회지
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• 제22권5호
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• pp.520-525
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• 1990

포화지방산 또는 불포화지방산의 분리방법인 요소부 가법이 지닌 가메탄올 분해(methanolysis)와 농축물 회수의 곤란함 등의 문제를 해결하기 위하여 지방산과 요소의 부가화합물 형성의 반응매질로 유기용매를 사용하고 요소의 습윤제로 물을 사용한 새로운 요소부가법을 제시하였다. 어유를 원료로 제조한 지방산을 헵탄, 헥산, 이소옥탄같은 비극성 유기용매에 용해키시고 요소와 요소의 습윤제인 물을 첨가하여 부가화합물 형성을 유도한 후 요소와 부가화합물을 잘 형성하지 않는 불포화지방산 분획을 회수하여 지방산 조성을 분석한 결과 EPA, DHA 및 이들의 전구물질을 포함한 고도불포화지방산이 80%이상인 것으로 나타났다. 이 고도불포화지방산 농축물내에 EPA와 DHA 함량은 사용하는 유기용매으 종류에 따라서 변화되었는데, 헵탄과 에틸 에테르 등을 사용할 경우에는 EPA의 농축에 효과가 높았다. 이러한 특성을 이용하여 우선 펜탄을 사용하여 DHA를 농축하고 다시 헵탄을 사용하여 EPA의 농축을 시도한 바 어유 지방산으로부터 DHA가 50%인 농축물과 EPA가 53%인 분획을 각각 얻을 수 있었다.

• 한국응용과학기술학회지
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• 제14권2호
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• pp.41-48
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• 1997

Separation of EPA and DHA from fish oil fatty acid ethyl ester (FAFE) by urea adductive crystallization method was carried out in the supercritical carbon dioxide (SC $CO_2$) as a solvent. Our results showed that SC $CO_2$ is a good candidate as a solvent in the urea adductive crystallization to separate FAFE by the number of unsaturated bonds. Compared to the separation process using methanol. SC $CO_2$ yielded better performance in the overall selectivity of EPA and DHA. The effect of process variables on separation of EPA and DHA was discussed in detailed. A hybrid technology of SC $CO_2$ fractionation and urea adductive crystallization with SC $CO_2$ was conformed as a viable process to separate and concentrate EPA and DHA from fish oil.

• Preventive Nutrition and Food Science
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• 제5권2호
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• pp.86-92
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• 2000

Conjugated linoleic acid(CLA) refers to a collective term of positional and geometric isomers of linoleic acid, which are different in their biological activities. The predominant isomer of CLA in animal tissues is cis-9, trans-11; smaller amounts of trans-10, cis-12 CLA isomers, CLA methyl ester (CLA-ME) was chemically syn-thesized from linoleic acid by the alkaline isomerization method. The synthetic CLA-ME, mainly composed of cis-9, trans-11 CLA and trans-10, cis-12 CLA, was dissolved in acetone, stored at 68$^{\circ}C$ for 1 day, and the supernatant(cis-9, trans-11 CLA-Me) was separated from the precipitate (trans-10, cis-12 CLA-Me). After the processes were repeated three times at -68$^{\circ}C$, the whole processes were repeated three times at -71$^{\circ}C$ in order to increase the purity of these two isomers. The cis-9, trans-11 CLA-Me and trans-10, cis-12 CLA isomers were further purified by the urea adduct. Purities of the cis-9, trans-11 CLA-Me and trans-10, cis-12 CLA-Me were 90.3 and 99.9%, respec-tively. This method could be employed for the preparation of a large quantity of highly purified cis-9, trans-11 CLA-Me or trans-10, cis-12 CLA-Me from synthetic CLA-Me.

• 한국응용과학기술학회지
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• 제10권1호
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• pp.57-65
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• 1993

The fatty acid, all $cis-{\Delta}^{5,11,14}-C_{20:3}$, in the Gingko nuts oils, was isolated and, purified by urea-adduct method, silver ion silica gel chromatography and HPLC equipped with reversed phase ${\mu}-Bondapak$ $C_{18}$ column. Its structural elucidation was conducted by IR and $^1H$-, $^{13}C$-NMR technique. The fatty acid composition of seed oils mainly consists of linoleic acid(37.73%), vaccenic acid(18.30%), oleic acid(15.18%), palmitic acid(3.37%), palmitoleic acid(3.37%) and ${\Delta}^5$ NMDB fatty acids(8.50%) in which all $cis-{\Delta}^{5,11,14}-C_{20:2}$ predominates.