• The Plant Pathology Journal
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• v.20 no.2
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• pp.131-134
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• 2004

A strain of Cucumber mosaic virus (CMV) was isolated from a weed, water chickweed (Stellaria aquatica), growing in the pepper field in Chunchon, Korea. This isolate, CMV-Sa, was differentiated from other CMVs based on biological properties and nucleotide sequence analysis of the coat protein (CP) gene. CMV-Sa showed different reactions to all the tested plants, except Capsicum annuum and Cucumis sativus, when compar-ed with those of CMV-Mf (subgroup I) and CMV-PaFM (subgroup II). Remarkably, in Nicotiana tabacum cvs. Samsun, Xanthi-nc and Ky-57, CMV-Sa induced local necrotic ring spots on the inoculated leaves and venal wave pattern and mosaic on the upper leaves. RNA analysis, serology, and RT-PCR of CP gene showed that CMV-Sa belonged to subgroup I of CMV. However, restriction enzyme analysis of the cDNA using AluI, HhaI, HincII, HindIII, HinfI and MspI showed that CMV-Sa was distinct from that of CMV-Mf. Based on comparison of the nucleotide of CP gene and deduced amino acid sequences between other CMV strains, CMV-Sa was closely related to CMV-Mf with 93.7% and 97.2 % identity, respectively.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2002.11a
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• pp.108.2-109
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• 2002

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2002.11a
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• pp.115-115
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• 2002

• The Korean Journal of Pesticide Science
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• v.8 no.4
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• pp.353-357
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• 2004

Large economic losses have been reported by root-knot nematide, Meloidogyne incognita, in Korea. However, fewy environmentally-friendly nematicide alternatives for the control of M. incognita have been developed. This study was conducted of Korean native plants to screen nematicidal activity which could be used by organic farmers. Methanol extracts (1,000 mg $L^{-1}$) from fifty-three Korean native plants were applied to 24-well tissue culture testplates containing $1\times10^2$ M incognita and the nematicidal activities were determined. Nematicidal activities of the methanol extracts in common purslain (Portulaca oleraceae L.) and water mouse-ear-chickweed (Stellaria aquatica Scop.) were 60.0% and 40.6%, respectively, compared with that of control, however, those of other forty-one plants were less than 30%. Our results suggest that the methanol extracts of common purslain and water mouse-ear-chickweed contain nematicidal active compounds.