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Effect of Micronutrient Supplementation on the Growth of Preschool Children in China

  • Han, Junhua;Yang, Yuexin;Shao, Xiaoping;He, Mei;Bian, Lihua;Wang, Zhu
    • Nutritional Sciences
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    • v.5 no.3
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    • pp.155-160
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    • 2002
  • The purpose of this study was to investigate the effects of micronutrient supplementation on the growth of preschool children in China. A double-blind, placebo-controlled trial was conducted on 156 growth retarded preschool children who were randomly assigned to the following five groups : supplemental control (S-control; n=28); zinc supplementation (+Zn; 3.5mg Zn/day, n=34); zinc and calcium supplementation (+ZnCa; 3.5mg Zn + 250mg Ca/day, n=37); zinc, calcium and vitamin A supplementation (+ZnCaVA; 3.5mgZn + 250mgCa + 200gVA/day, n=28); and calcium and vitamin A supplementation (+CaVA; 250mgCa + 200gVA/day, n=29). Another 34 children of normal height were selected as a normal control (N-control). Supplementation continued for twelve months. After supplementation, the height gains in the +Zn group (7.84cm per year) and the +ZnCa group (7.70 cm per year) were significantly higher than that in the S-control group (6.74 cm per year, P<0.05). The weight gain in the +ZnCaVA group (2.55kg per year) and the +CaVA group (2.57 kg per year) was also significantly higher than that in the S-control group (2.19 kg per year, P<0.05). The average number of days of illness in each group taking supplements was lower than that in the S-control group (13 days per year compared with 23 days per year). No significant differences in bone maturity were observed between the groups. In conclusion, in this study Zinc and Zinc + Calcium supplementation improved the height gain, and vitamin A improved the weight gain, in growth retarded preschool children, but these supplements did not affect the maturity of bone. Micronutrient supplementation also lowered the morbidity of these children.

Effects of Ethanol on Zinc, Calcium, Copper and Magnesium Conoentrations of Tissues in Rats (Ethanol 투여가 체내의 Zn, Ca 및 Mg의 함량에 미치는 영향)

  • Lee, Hyun Beom;Lee, Keun Woo;Hong, Kyung Tae
    • Current Research on Agriculture and Life Sciences
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    • v.7
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    • pp.221-230
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    • 1989
  • Present experiment was designed to investigate the effect of long-term ethanol drinking on the mineral contents on hair, liver, kidney and muscle tissues. Forty-five rats were divided into 2 experimental groups and a control group. The control group received tap water and the other 2 groups 4% and 25% ethanol respectively as drinking source for 28 days. All rats were dissected on the 29th day. Hair, liver, kidney and muscle samples were taken and analyzed for zinc, calcium, copper and magnesium contents by atomic absorption spectrophotometeric method. The results obtained are summrized as follows : 1) The zinc content of muscle in 25% ethanol group showed significant (P<0.01) decrease whereas that of hair in 4% ethanol group showed significant (p<0.1) increase. 2) The calcium content of hair in 4% ethanol group showed significant (p<0.1) increase whereas; that of hair, kidney and muscle in both group showed no significant difference. 3) The copper and magnesium contents of muscle showed significant (p<0.1, p<0.05) decrease in both groups. From these results it may be concluded that the long-term drinking of ethanol affects the mineral content of body tissue.

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Levels of Serum Lipids, Copper, Zinc, Ceruloplasmin and Ferroxidase Activity in Smoking College Women (흡연 여대생의 혈청 지질, Cu, Zn, Ceruloplasmin 농도 및 Ferroxidase 활성도)

  • 김정희;이화신
    • Korean Journal of Community Nutrition
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    • v.2 no.4
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    • pp.515-522
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    • 1997
  • This study was done to compare serum levels of lipids, trace minerals, ceruloplasmin and ferroxidase activity between female college smokers and nonsmokers. Serum levels of lipids, Cu, Zn, ceruloplasmin and ferroxidase activity were determined in 33 smokers and 42 nonsmokers residing in the Seoul area. Serum levels of TG, total cholesterol and HDl-cholesterol were measured using test kits. Serum Cu Zn were determined by the AAS. Serum ceruloplasimin level and ferroxidase activity were determined by spectrophotometric methods. All data were statistically analyzed by the SAS PC package program. Mean and standard errors were calculated for each item, and the significance of the difference between two groups was evaluated by Student's t-test at p〈0.05. There was no significant difference in levels of total cholesterol, TG, VLDL-cholesterol, LDL-cholesterol, LDL/HDL and atherogenic index, but HDL-cholesterol level was significantly lower(p$\ll$0.05) in female college smokers than in nonsmokers. Serum zinc level showed no significant difference, whereas copper level was significantly higher in smokers, consequently yielding higher Cu/Zn ratio in smoking college women. Ceruloplasmin level was 32.6mg/dl in smoking college women, which was significantly higher(p$\ll$0.05) than that of 28.6mg/dl in nonsmokers. However, cerulopasmin ferroxidase activity, 0.34U/mg in nonsmokers was higher(p$\ll$0.05) than that of 0.29U/mg in smokers. From these results, it is confirmed that cigarette smoking itself has a very detrimental effect on health and mineral nutritional status although the smoking history is relatively short among college women(pack-years=1.7). (Korean J Community Nutrition 2(4) : 515-522, 1997)

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Evaluation of Heavy Metal Pollution in the Dumping Site of the Dredged Sediment, Masan Bay (마산만 오염퇴적물 준설토 투기해역의 중금속 오염평가)

  • Kwon Young-Tack
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.7 no.2
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    • pp.75-81
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    • 2004
  • A large amount 2.1×106 ㎥ of the polluted sediment was dredged from the Masan Bay and deposited in Gapo confined area, Masan. The dissolved metal concentrations of seawater in the dumping site (Gapo area) were observed during one tidal cycle and compared with those of seawater obtained from Jinhae Bay. The sediment was evaluated as from Non polluted to Moderately polluted by USEPA standards. It was judged that toxicological effects of sediment analyzed ranged from ERL to ERM with copper and zinc, and ERL with cadmium, chrome, lead, and nickel by the Adverse Biological Effects. The pollutant concentration was low in surface sediment compared to deeper sediment since the sediments with relatively low concentrations of pollutant were dumped to the surface. The pollutant concentration was low in surface sediment compared to deeper sediment since the sediments with relatively low concentrations of pollutant were dumped to the surface. The benthic organisms in Gapo area had higher concentrations of trace metals (Oyster: Zn 238.96, Cu 5.29 ㎍/g wet wt., Clam: Zn 17.71, Cu 1.00 ㎍/g wet wt., Mussel. Zn 187.98, Pb 0.28, Cr 0.15, Mn 4.23, Sr 1.45 and Fe 100.33 ㎍/g wet wt.) compared to outside of dumping site. However, the trace metal level in the bivalves was less than the NFPQIS (National Fisheries Products Quality Inspection Service) standard.

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Pollution of Heavy Metals and Sedimentation Rate in the Sediments of Suyeong Bay, Pusan (수영만 퇴적물의 퇴적속도와 중금속 오염)

  • YANG Han-Soeb;KIM Seong-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.5
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    • pp.643-658
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    • 1994
  • The sedimentary records of anthropogenic metal loads in the Suyeong Bay, Pusan were determined by combining the Pb-210 dating technique with the measurements of heavy metals in the sediment cores. The sedimentation rates of sediment particles ranged from $0.12\;to\;0.20\;g/m^2/yr\;or\;2.4{\sim}4.0\;mm/yr$ in accumulation rates. The lowest sedimentation rate was observed at station S3 which was characterized by a bottom with relatively low organic matter contents(e.g. TIL and TOC). Heavy metals showed generally higher concentrations at station S1 and S2 near the mouth of the Suyeong River than at station S3 and the outmost station S4. The contents of copper, lead and zinc in the sediment cores especially from station S1 and S2 began to increase around 1930, and were at their highest levels in the $1960{\sim}1970$ period as a result of increasing industrial activities. Concentrations of these heavy metals have slightly decreased since 1970, probably due to regulation of pollution discharge. The natural background levels of copper, lead and zinc in the sediments of this bay ranged $18{\pm}4ppm,\;28{\pm}6ppm\;and\;74{\pm}9ppm$, respectively, by averaging the contents in the sediment depths corresponding to periods between about 1900 and 1920 at the four stations. The total amounts of anthropogenic loads deposited in the sediments since about 1930 were estimated to be $9{\sim}291{mu}g/cm^2$ for lead, $165{\sim}1122{mu}g/cm^2$ for zinc and $20{\sim}208{mu}g/cm^2$ for copper. These values were remarkably high at stations S1 and S2 relative to the other two stations. At stations S1 and S2, the anthropogenic loads of lead, copper and zinc constituted $29{\sim}30\%,\;32{\sim}42\%\;and\;28{\sim}35\%$ of the total sedimentary inventories at the present day, respectively. These metal contents have a good correlation(r>0.7) with each other and cadmium measurements also show a positive linear relation with nickel or total organic nitrogen.

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Stannite from the Janggun Mine, Republic of Korea -Contributions to the Knowledge of Ore-Forming Minerals in the Janggun Lead-Zinc-Silver (3)- (한국(韓國) 장군광산(將軍鑛山)의 황석석(黃錫石)에 대(對)하여 -장군(將軍) 연(鉛)·아연(亞鉛)·은(銀) 광석광물(鑛石鑛物)의 지식(知識)에의 기여(寄與) (3)-)

  • Lee, Hyun Koo;Imai, Naoya
    • Economic and Environmental Geology
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    • v.19 no.spc
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    • pp.121-130
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    • 1986
  • In the Janggun mine, stannite occurs as anhedral grains, up to 500 micrometer in long dimension, closely associated with sphalerite, chalcopyrite, arsenopyrite, pyrrhotite, galena and rhodochrosite in the periphery of the South ore body. In reflected light, stannite is grayish yellow green in color and exhibits moderate bireflectance and strong anisotropism without any intenal reflections. Reflection; Rmax. =29.0, Rmin. =27.8 percent at a wavelength of 560nm, and VHN; 219~244kg/mm at a 50g load. The chemical composition on the average from 35 spot analyses by electron microprobe is, Cu 28.0, Fe 12.7, Zn 2.9, Mn 0.2, Sn 25.8, S 30.3, sum 99.9 (all in weight percent); the corresponding chemical formula as calculated on the basis of total atoms=8 is, Cu 1.88 Fe 0.97 Zn 0.19 Mn 0.02 Sn 0.93 S 4.01, which fulfills approximately the ideal formula of $Cu_2FeSnS_4$. The strongest reflections on the X-ray diffraction patterns are; $3.10{\AA}$ (10) (112), $2.72{\AA}$ (5) (020, 004), $1.922{\AA}$ (5) (024), $1.642{\AA}$ (3) (132), $1.244{\AA}$ (3) (143, 136, 235), $1.111{\AA}$(3) (244), $0.958{\AA}$ (1) (048, 422), the patterns are identical with those of literature. From the textural evidence of the microscopic observation, the mineral is considered to have been formed at the middle stage of hydrothermal lead-zinc-silver mineralization.

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The Characteristics of Heavy Metal(Zn, Pb) Accumulations in Paridae Nesting Material (박새과 조류의 둥지 재료 내 중금속(Zn, Pb) 축적특성 연구)

  • Kyeong-Tae Kim;Hyun-Jung Lee;Whee-Moon Kim;Won-Kyong Song
    • Korean Journal of Environment and Ecology
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    • v.36 no.6
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    • pp.566-574
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    • 2022
  • Heavy metals are one of the dangerous pollutants that threaten urban biodiversity due to their accumulation over a long period without being decomposed in vivo. Accordingly, there is a need for biological monitoring to compare accumulation concentrations in living organisms according to the degree of heavy metal exposure to evaluate heavy metal contamination in the air in urban areas. This study aims to examine the possibility of using nesting materials as heavy metal monitoring samples and determine the effects of heavy metals on Paridae. We installed 54 artificial nest boxes in the research areas that included campus green spaces (14), urban forests (11), and urban parks (29) on a university campus in Cheonan City, Chungcheongnam Province. The birds' use rate of artificial nest boxes was 11/14 (78.57%) in campus green spaces, 8/11 (72.72%) in urban forests, and 6/29 (20.68%) in urban parks. Moss materials were collected from collected nests, and the heavy metal accumulation characteristics of each type of urban green space and the effects of heavy metals on the success of fledging of Paridae were compared through heavy metal analysis. The analysis showed that the average concentrations of zinc and lead were 228.08±209.62 ㎍/dry g and 17.67 ± 6.72 ㎍/dry g, respectively. There was no significant difference in zinc concentration for each type of urban green space (Kruskal-Wallis test, p-value=0.28), but lead concentration showed a significant difference (Kruskal-Wallis test, p<0.05*). Of the 21 Paridae, nests analyzed for heavy metals, fledging of birds was observed in 11 nests (52.38%). Fledging of birds observed in each urban green space type was 7 campus green spaces (77.78%), 6 in urban forests (85.71%), and 1 in urban park (20%), mainly in urban forests and green spaces on campus. Heavy metal concentrations were compared to check the effect of heavy metal accumulation on the successful fledging of Paridae, but there was no statistically significant difference (Zn: W=44, p-value=0.74, Pb: t=0.64676, df =7.2422, p-value=0.54). This study is a basic study using the nesting materials of Paridae as heavy metal monitoring samples, and it is determined that it can be used as basic data for non-invasive biological monitoring.

Dietary intakes of Fe, Zn, Cu, Mn, Se, Mo, and Cr of Korean adult women - Comparison between the data from analyzed and calculated - (성인 여성의 미량무기질(Fe, Zn, Cu, Mn, Se, Mo 및 Cr) 섭취량 - 분석치와 계산치의 비교 -)

  • Kim, Kyung-Hee;Lim, Hyeon-Sook
    • Korean Journal of Human Ecology
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    • v.9 no.3
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    • pp.69-79
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    • 2006
  • The previous studies on the intake of trace elements performed in Korea were only concerned about major elements like Fe, Zn or Cu. There is little data about the intake of minor elements like Mn, Se, Mo or Cr. And most of the data were obtained by calculation using Food Composition Tables or by analysis using atomic absorption spectrophotometers (AAS). The purpose of this study, therefore, was to evaluate the dietary intake of seven trace elements, Fe, Zn, Cu, Mn, Se, Mo, and Cr of Korean adult wonmen and to compare the data between analyzed using ICP-MS (or ICP-AES) and calculated by the CAN-Pro (or Food Composition Table). A total of nineteen adult women participated voluntarily in this study and collected one-tenth of the foods that they consumed for three consecutive days. Analyzed intake of Fe of the subjects was $6.94{\pm}2.18$ (calculated, $18.87{\pm}4.50$) mg/day, that of Ze was $9.35{\pm}4.95$ (calculated, $8.35{\pm}2.87mg/day$), that of Cu was $1.18{\pm}0.26\;(1.11{\pm}0.32mg/day)$, that of Mn was $3.69{\pm}0.69\;(2.83{\pm}1.68mg/day)$, that of Se was $41.93{\pm}9.28$ (calculated, $27.58{\pm}6.97{\mu}g/day$), that of Mo was $134.0{\pm}49.1{\mu}g/day$, and that of Cr was $136.5{\pm}147.9{\mu}g/day$. The analyzed Fe intake of the subjects did not meet Estimated Average Requirement (EAR) nor Recommended Intakes (RI) for Korean women aged 20-29 years old. However, the analyzed intakes of the other six elements, Zn, Cu, Mn, Se, Mo, and Cr, seemed to meet each of the respective RIs. The analyzed Fe intake was significantly lower than the calculated one, in fact by 2.72 times, however, the analyzed intakes of the other elements, Mn and Se, were significantly higher and those of Zn and Cu were similar than each of the calculated ones. The difference of the data between analyzed and calculated intakes indicates that it is necessary to set up database on trace element contents of foods of the Food Composition Table and the CAN-Pro so as to have accuracy.

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Dietary Intakes, Serum Concentrations, and Urinary Excretions of Fe, Zn, Cu, Mn, Se, Mo, and Cr of Korean Young Adult Women (일부 젊은 성인여자의 Fe, Zn, Cu, Mn, Se, Mo 및 Cr의 식사섭취, 혈청농도 및 소변배설)

  • Kim, Kyune-Hee;Lim, Hyeon-Sook
    • Journal of Nutrition and Health
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    • v.39 no.8
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    • pp.762-772
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    • 2006
  • This study was conducted to investigate dietary intakes, serum concentrations, and urinary excretions of iron (Fe), zinc (Zn), copper (Cu), manganese (Mn), selenium (Se), molybdenum (Mo), and chromium (Cr) of Korean young adult women. A total of 19 apparently healthy young adult women aged in their twenties or thirties participated voluntarily. One-tenth of all foods they consumed for 3 consecutive days were collected, all urine excreted for the same 3 days was gathered, and fasting venous blood was withdrawn for the trace mineral analyses. Of the food, blood, and urine samples, the contents of Zn, Cu, Mn, Se, Mo, and Cr were analyzed by inductively coupled plasma-mass spectroscopy (ICP-MS) and that of Fe by inductively coupled plasma-atomic emission spectroscopy (ICP-AES) after wet digestion. The intake of Fe, $6.94{\pm}2.18mg$, did not meet the estimated average requirement (EAR) for Korean women aged 20-29 years old. On the contrary, the intakes of Zn ($9.35{\pm}4.95mg$), Cu ($1.18{\pm}0.26mg$), and Mn ($3.69{\pm}0.69mg$) were sufficient for each respective EAR. However, some of the subjects did not take the EAR for Zn. The Se intake, $41.93{\pm}9.28{\mu}g$, however, was almost similar to the EAR for Se. Although there are no references for Mo and Cr, the intakes of these minerals ($134.0{\pm}49.1\;and\;136.5{\pm}147.9{\mu}g$, respectively) seemed to be excessively sufficient. Serum Fe concentration, $88.7{\pm}36.8{\mu}g/dL$, seemed to be a little bit lower than its reference median but within its normal range. Approximately one-fourth of the subjects were in anemic determined by Hb and Hct and below the deficiency serum level of Fe, $60{\mu}g/dL$. In addition, serum Se concentration, $3.73{\pm}0.60{\mu}g/dL$, was also below its reference median and normal range. However, serum concentrations of Zn ($99.6{\pm}30.6{\mu}g/dL$) and Mo ($0.25{\pm}0.10{\mu}g/dL$) were fairly good compared to each reference median. The status of Cu could be determined as good although its serum concentration ($91.6{\pm}14.6{\mu}g/dL$) was slightly below its reference median. Since there are no decisive reference values, it was not easy to evaluate serum concentrations of Mn ($0.93{\pm}0.85{\mu}g/dL$) and Cr ($8.60{\pm}7.25{\mu}g/dL$). But Mn and Cr status seemed to be adequate. Urinary Fe excretion, $4.48{\pm}1.98{\mu}g/dL$, was pretty much lower than its reference and that of Se, $2.45{\pm}1.17{\mu}g/dL$, was also lower than its average. On the other hand, those of Zn ($42.95{\pm}20.47{\mu}g/dL$) and Cu ($5.68{\pm}1.50{\mu}g/dL$) were flirty good. In case of Mn, urinary excretion, $0.31{\pm}0.09{\mu}g/dL$, was much greater than its reference. Urinary excretions of Mo ($7.48{\pm}2.95{\mu}g/dL$) and Cr ($1.37{\pm}0.41{\mu}g/dL$) were very higher compared to each reference. The results of this study revealed that Korean young adult women were considerably poor in Fe status, a bit inadequate in Se status, partly inadequate in Zn status, and flirty good in Cu, Mn, Mo and Cr status. However, there was a problem of excessive intakes of Mo and Cr. It, therefore, should be concerned to increase the intakes of Fe, Se and Zn but to decrease Mo and Cr consumption for young adult women.

Analysis of Paint used in Streetcar No. 381 of Registered Culture Property (등록문화재 전차381호의 도료 분석)

  • Kim, Soo Chul;Park, Min Soo;Seo, Jeong Hun
    • Journal of Conservation Science
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    • v.28 no.3
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    • pp.277-283
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    • 2012
  • This study conducted analysis of paint samples from a streetcar No. 381 (Registration Cultural Property No. 467) to identify the characteristics of the modern paint. The samples were analyzed by Microscopic observation, infrared spectroscopy and SEM-EDS. The upper part is consist of 26 layers and the under part is 29 layers. And the layers were painted various thickness from $10{\mu}m$ to $100{\mu}m$, and confirmed that several color had been used. The putty was used for surface treatment before painting. According to the results of infrared spectroscopy, paint specimens were identified as alkyd resin. Some bands such as C-H and C=O stretcing, aromatic, C-H bending, C-O stretching were found in spectra. Inorganic analysis showed that gold color on the surface was used copper-based paint. And type of the putty was lacquer-putty that was consist of kaolin, talc and zinc white.