A Segmentation Method for Counting Ammonia-oxidizing Bacteria

암모니아산화세균의 계수를 위한 영상분리기법

As a method to control the bacteria number in adequate level, a real time control system based on microscope image processing measurement for the bacteria is adopted. For the experiment, Ammonia-oxidizing bacteria such as Acinetobacter sp. are used. This paper proposed hybrid method combined watershed algorithm with adaptive automatic thresholding method to enhance segmentation efficiency of overlapped image. Experiments was done to show the effectiveness of the proposed method compared to traditional Otsu's method, Otsu's method with adaptive automatic thresholding method and human visual method.