한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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  • 김준홍 (전남대학교, 물질, 생물화공과, 화학공학부, 한국에너지기술연구원, 바이오매스연구팀) ;
  • 이종일 (전남대학교, 물질, 생물화공과, 화학공학부, 한국에너지기술연구원, 바이오매스연구팀) ;
  • 김미선 (전남대학교, 물질, 생물화공과, 화학공학부, 한국에너지기술연구원, 바이오매스연구팀)
  • Published : 2001.11.07
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Abstract

On-line monitoring technique for the concentration of lactate by a FIA(Flow Injection Analysis) system was studied. The lactate oxidase(LOD) was immboilized on VA-Epoxy carrier and integrated into the FIA system. The pH, buffer flow rate and temperature for the LOD-FIA were optimized, and the effects of salts and metabolites dissolved in the sample on the activity of immobilized enzyme were investigated. The LOD-FIA has been applied to monitor the concentrations of lactate in a simulated bioprocess. The on-line monitoring data by the LOD-FIA agreed with the off-line data measured by a fluorescence spectroscopy well.

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