Proceedings of the SCSK Conference (대한화장품학회:학술대회논문집)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effects of N-acetylphytosphingosine on melanogenesis of B16F10 murine melanoma cells.

  • Park, M. K. (Bioanalysis and Biotransformation Research Center, Koera Institute of Science and Technology) ;
  • Park, C. S. (Doosan Corp. Biotech BU, Biotech Res. Lab) ;
  • Kim, J. W. (Doosan Corp. Biotech BU, Biotech Res. Lab) ;
  • R. M. Ahn (Department of Applied Chemistry, Dongduk Women′s Universit) ;
  • Y. S. Yoo (Bioanalysis and Biotransformation Research Center, Koera Institute of Science and Technolog) ;
  • S. Y. Yi (Department of Applied Chemistry, Dongduk Women′s University)
  • Published : 2003.09.01
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Abstract

The effects of N-acetylphytospingosine(NAPS), one of the phytospingosine derivatives, on melanogenesis of B 16F 1 0 mouse melanoma cell lines were investigated. We assessed the effect of NAPS on the depigmentation of B16F10 cells. The melanin content of cells was significantly reduced by NAPS. We examined the inhibitory effect of NAPS on tyrosinase activity using L-dopa as a substrate and the results showed that tyrosinase activity was inhibited in a does-dependent manner. The mRNA level of tyrosinase as well as that of tyrosinase related protein-l (TRP-l) and tyrosinase related protein-2 (TRP-2) genes were not affected by NAPS based on a reverse transcription-polymerase chain reaction (RT-PCR) assay. We also performed a Western blotting analysis using anti-tyrosinase antibody. It showed that there is no change in tyrosinase protein level after treatment of NAPS. These results suggest that the depigmenting mechanism of NAPS in B16F10 melanoma cells involves inhibition of melanosomal tyrosinase activity, rather than the mRNA expression or protein level of tyrosinase.

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