Proceedings of the SCSK Conference (대한화장품학회:학술대회논문집)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지

The study on collagen sysnthesis and collagenase inhibition assay in natural plants

  • W. J. Yang (Korea Advanced Food Research Institute) ;
  • S. J. Yang (Korea Food and Drug Administration, Dep. Of Drug evaluatio) ;
  • Kim, W. H. (Korea Food and Drug Administration, Dep. Of Drug evaluatio) ;
  • T. B. Kang (Sang-myung universit) ;
  • Park, S. S. (Korea Food and Drug Administration, Dep. Of Drug evaluation)
  • Published : 2003.09.01
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Abstract

Type I (collagen) and procollagen are reduced in aged human skin. This reduction could result from increased degration by metalloproteinases and from reduced procollagen synthesis and skin collagenase is required for initiation of the degration of type I collagen. In the present study, we study on assay the collagen and collagenase in natural plants using the fibroblast human skin cell. We select the 15 kind of plants used to herbal and 4 kind of fraction(by methylene chloride, ethyl acetate, n-butanol, water). Among these extract, the ethyl acetate fraction from benincasa hispida/prunus persica, trichosanthes kiriowii, trogopterus xanthipes and methylene chloride fractions from benincasa hispida/prunus persica, torilis japonica and n-butanol fraction from cnidium officinale, chrysanthemum sibiricum were selected for further experiments as they exhibited distinctive amount of collagen compared to other natural extracts. These extracts were again subjected to collagenase assay test. Benincasa hispida/prunus persica extract was shown to have exellent collagen synthesis activity from result of the collagen assay test and the other extract was shown to have over 130% of collagen synthesis activity. But, in the study of collagenase assay test just only trogopterus xanthipes extract was shown to have collagenase inhibition.

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