Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
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Effects of (lR,9S)-($\beta$)-Hydrastine on Intracellular Calcium Concentration in PC12 Cells

  • Kim, Yu-Mi (College of Pharmacy, Chungbuk National University) ;
  • Lee, Jae-Joon (College of Pharmacy, Chungbuk National University) ;
  • Jin, Chun-Mei (College of Pharmacy, Chungbuk National University) ;
  • Yang, Yoo-Jung (College of Pharmacy, Chungbuk National University) ;
  • Yin, Shou-Yu (College of Pharmacy, Chungbuk National University) ;
  • Kang, Min-Hee (College of Pharmacy, Chungbuk National University) ;
  • Lee, Myung-Koo (College of Pharmacy, Chungbuk National University)
  • Published : 2003.10.01
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Abstract

(1R,9S)-(${\beta}$)-Hydrastine (HS) at 10-50 ${\mu}$M has been proven to have an inhibitory effect on dopamine biosynthesis in PC12 cells by the inhibition of tyrosine hydroxylase (TH) activity and TH gene expression. In the present study, therefore, the effects of HS on the basal and K$\^$+/-induced dopamine release, and Ca$\^$2+/ influx induced by high K$\^$+/ and caffeine in PC12 cells were investigated. The dopamine release by high K$\^$+/ (56 mM) was inhibited by co-incubation of 20 ${\mu}$M HS. Application of HS also significantly reduced the magnitude of the maintained Ca$\^$2+/ influx induced by K$\^$+/ depolarization. (omitted)

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