Proceedings of the Korean Institute of Information and Commucation Sciences Conference (한국정보통신학회:학술대회논문집)
- 2015.10a
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- Pages.838-841
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- 2015
A Novel Possibility of Recombinant Baculovirus Vector
재조합 베큘로바이러스 벡터의 새로운 가능성
- Kim, Ji-Young (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
- Kim, Hyun Joo (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
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Hong, Seong-Karp
(Division of Bio and Health Sciences, Mokwon University)
- Published : 2015.10.26
Abstract
Recombinant baculovirus vector is composed of genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD). This recombinant baculovirus vector was transfected into cell lines and tissues and then found out a novel possibility in view of gene transfer and gene expression in comparison to other vector systems. Efficacy of gene transfer and gene expression of this recombinant baculovirus vector was higher than any other vector system.
재조합 베큘로바이러스는 polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) 유전자로 구성된다. 본 재조합 베큘로바이러스 벡터는 세포주와 조직에 감염시켰고 그 결과 다른 벡터 시스템에 비교하여 재조합된 유전자의 전이와 유전자 발현에 있어서 새로운 가능성이 발견되었다. 본 재조합 베큘로바이러스 시스템의 유전자의 전이와 발현의 효율은 타 벡터시스템 보다 우수하였다.