Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)
- 2018.04a
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- Pages.80-80
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- 2018
Anti-adipogenic Effect of Chlorogenic Acid in 3T3-L1 Adipocytes
- Park, Se-Eun (Department of Food Science and Biotechnology, Gwangju University) ;
- Choi, Jun-Hui (Department of Food Science and Biotechnology, Gwangju University) ;
- Lee, Hyo-Jeong (Department of Food Science and Biotechnology, Gwangju University) ;
- Seo, Kyoungsun (Jangheung County Mushroom Research Institute) ;
- Kim, Seung (Department of Food Science and Biotechnology, Gwangju University)
- Published : 2018.04.27
Abstract
Chlorogenic acid is a phenolic compound found in Cudrania tricuspidata fruits. In the present study, the effect of chlorogenic acid on the inhibition of adipogenesis in 3T3-L1 adipocytes was investigated. Cells were stained with Oil red O reagent to detect lipid droplets in adipocytes. The 3T3-L1 cells were lysed and measured for intracellular triglyceride and adipokine by ELISA kit. The protein expression of adipogenesis-related gene was evaluated by Western blot analysis. Chlorogenic suppressed lipid droplet and intracellular triglyceride accumulation in a concentration manner and also decreased secretion of adipokines such as leptin and adiponectin, compared with fully differentiated adipocytes. Treatment of 3T3-L1 cells with chlorogenic acid reduced the protein levels of peroxisome proliferator-activated receptor gamma (