한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제3권1호
- /
- Pages.1-5
- /
- 1971
- /
- 0367-6293(pISSN)
Myriococcum albomyces에 있어서 Cellulase 유도생성에 관한 연구
Studies on Cellulase Induction in Myriococcum albomyces
- 정동효 (건국대학교 공과대학 발효공학과)
- Chung, Dong-Hyo (Department of Fermentation Technology College of Engineering, Kon-Kuk University)
- 발행 : 1971.04.28
초록
1. 5% 밀기울 기본배지에다 inducer로서 CMC 혹은 Avicel을 첨가하여 Myriococcum albomyces를 접종하여 진탕배양할 때의 cellulase 유도 생성을 검토하였다. 2. DEAE-Sephadex A-25 column chromatography 법으로 배양액에서 cellulase 활성이 다른 3개의 부분, fraction I, fraction II 와 fraction III를 분리하였다. 3. Inducer로서 CMC첨가의 경우는 CMCase가 강해지고 반대로 Avicel을 가한 경우는 Avicelase가 강하였다.
1. Formation of cellulase in Myriococcum albomyces was investigated using shaking culture with addition of CMC or Avicel as an inducer to 5% wheat bran medium. 2. Three different types of cellulase fraction I, fraction II and fraction III in the culture filtrate were purified by elution column chromatography on a DEAE-Sephadex A-25. 3. By the addition of CMC as an inducer, CMCase activity was stronger than that of Avicelase. On the other hand, the addition of Avicel increased Avicelase activity.
키워드