Studies on Culture of Leptospires On Growth of Leptospires in Cox's Liquid Media and Korthof's Media, Added Rabbit Serum and Thiamine Hydrochloride, Inactivated at High Temperature

Leptospira 균(菌) 배양(培養)에 관한 연구(硏究) -가열처리(加熱處理)한 토끼 혈청가배지(血淸加培地)와 Thiamine 첨가배지(添加培地)에서의 Leptospira 균(菌)의 발육(發育)에 관하여-

The rate of growth of Leptospires were studied by culturing in Korthof's media and Cox's liquid media which were treated at four different temperatures. The effect of thiamine hydrochloride in the culture of leptospires were also studied. The results obtained were summerized as followings: 1. The best growth of Leptospira icterohaemorrhagiae and Leptospira canicola was observed in Korthof's media with the rabbit serum heated for 60 minutes at $56^{\circ}C$. 2. A good degree of growth of Leptospira icterohaemorrhagiae and Leptospira canicola was observed in both Cox's liquid media with rabbit serum heated for 30 minutes at $100^{\circ}C$ and with fresh rabbit serum. 3. The growth of Leptospira was enhanced by adding small amount of thiamine to the both media which were shown to be unsatisfactory for the growth of the organisms. 4. Culture of leptospires were attained by simply heating for 30 minutes at $100^{\circ}C$ both Korthof's media and Cox's liquid media containing rabbit serum obtained without aspetic procedures.