Studies on the Anti-aging Action of Korean Ginseng (I) Comparative Study of Red and White Ginsengs on Anti-aging Action

고려인삼(高麗人蔘)의 노화억제작용(老化抑制作用)에 관한 연구(硏究) 제1보(第1報) 홍삼(紅蔘)과 백삼(白蔘)의 노화억제작용(老化抑制作用) 비교연구(比較硏究)

The inhibitory effects of the extract and crude saponin of red and white ginsengs on lipoperoxide formation in vitro and in vivo were studied and correlated with anti-aging. To this end, antioxidant activity, induction period and lipoperoxide were measured by the methods of EDA, POV and TBA value. And also superoxide dismutase and peroxidase activity were measured by pyrogallol autoxidation method (${\Delta}A$ 420/min) and initial velocity(${\Delta}A$ 436/min), respectively. From HPLC analysis, the PT/PD ratio of red and white ginsengs was found to be 0.561% and 0.401%, respectively, and red ginseng increased the PT/PD ratio in comparison with white ginseng. The EDA activity of red ginseng was higher than that of white ginseng; red ginseng showed stronger antioxidative effect than white ginseng. The inhibitory effect of red ginseng was lower than that of white ginseng during the induction period. It was proved that high molecular coloring substance was deeply related to the initial stage of lipoperoxidation. There was no significant difference between red and white ginsengs in both in vitro and intraperitoneal administration experiments, and red ginseng was more effective than white ginseng in longterm administration. And also inhibitory effect on lipoperoxide formation was mainly occurred in liver, suggesting that the function of liver played an important role in anti-aging actions. From the measurement of superoxide dismutase(SOD) activity for both ginseng groups intraperitoneally and orally administered, it was found that red ginseng group administered extract and crude saponin showed remarkable inhibitory effects in comparison with white ginseng. In particular, orally administered group showed more stronger inhibitory effect on lipid peroxidation in comparison with intraperitoneally administered group. It was also found that the continuous oral administration was more effective than temporary administration. Red ginseng was more notable anti-aging effect in comparison with white ginseng in vivo, and this may be due to the increase of SOD activity in rat-liver. Peroxidase activity also showed similar trend to SOD activity in vitro and in vivo experiments. Red ginseng was not only superior to white ginseng for preservation but also for biochemical and pharmaceutical efficacy.

홍삼(紅蔘)과 백삼(白蔘) 항산화작용(抗酸化作用)에 의한 노화억제작용(老化抑制作用)을 구명(究明)하기 위하여 80% ethanol extract 및 수포화(水飽和)부탄올로 이행(移行)한 saponin을 시료(試料)로 하여 in vitro 및 in vivo 실험(實驗)을 통하여 홍삼(紅蔘)과 백삼(白蔘)의 노화억제작용(老化抑制作用)을 비교(比較)하였다. DPPH에 대한 전자공여능(電子供與能)(EDA), TBA value 및 peroxide value에 의한 과산화지질생성억제작용(過酸化脂質生成抑制作用), superoxide dismutase 및 peroxidase의 활성측정(活性測定)등으로 홍삼(紅蔘)과 백삼(白蔘)의 노화억제작용(老化抑制作用)을 비교(比較)한 결과(結果), 홍삼(紅蔘)은 장기간(長期間)에 걸친 연속투여(連續投與)에서 백삼(白蔘)보다 과산화지질생성억제작용(過酸化脂質生成抑制作用). superoxide dismutase 및 peroxidase의 활성(活性)을 유의성(有意性)있게 증가(增加)하였다. 생체내실험(生體內實驗)에서 홍삼(紅蔘)이 백삼(白蔘)보다 노화억제작용(老化抑制作用)이 현저한 것은 증삼(蒸參) 및 가공처리중(加工處理中) saponin pattern의 변화(變化)에 기인(起因)하며 백삼(白蔘)의 PT/PD ratio가 0.401인데 반(反)해 홍삼(紅蔘)의 PT/PD ratio가 0.561로서 triol saponin이 증가(增加)되었기 때문이다.