Purification and Properties of the Factor from Arthrobacter luteus, Capable of Accelerating the Lysis of Yeast Cell Walls

Arthrobacter luteus가 생산(生産)하는 효모(酵母) 세포벽(細胞壁) 용해(溶解) 촉진인자(促進因子)의 정제(精製) 및 그 이화학적(理化學的) 성질(性質)

The factor, which was capable of accelerating the yeast cell wall lysis of the zymolyase(${\beta}-1$, 3-glucanase), was purified to a homogeneous state from the protease fraction of the crude zymolyase by Sephadex G-75 gel filtration and preparative polyacrylamide gel disc electrophoresis. The molecular weight of the purified factor was estimated to be 40,500 by SDS-polylacrylamide gel disc electrophoresis and it's iso-electric point was pH 9.6. The factor was found to be a basic protease consisted of single polypeptide chain with 395 amino acid residues and it showed the $E_{280,cm}^{1%}$ of 11.9 and the molecular extinction coefficient of $4.83{\times}10^4$, respectively.

zymolyase(${\beta}-1$, 3-glucanase)의 효모(酵母) 세포벽(細胞壁)에 대한 용해작용(溶解作用)을 촉진(促進)시키는 인자(因子)를 zymolyase의 조효소(粗酵素)로부터 분리(分離)하여 Sephadex G-75 gel 여과(濾過) 및 조제용(調製用) PAG-전기영동법(電氣泳動法)에 의하여 전기영동적(電氣泳動的)으로 균일(均一)하게 정제(精製)하였다. SDS-PAG 전기영동법(電氣泳動法)에 의하여 측정한 정제(精製) 인자(因子)의 분자량(分子量)은 40,500이었고, 등전점(等電點)은 pH 9.6이었다. 정제(精製)된 촉진인자(促進因子)는 395개(個)의 아미노산 잔기(殘基)로 구성(構成)되는 단일(單一) polypeptide의 염기성(鹽基性) protease임이 밝혀졌으며, 이 protease의 흡광계수(吸光係數)($E_{208,cm}^{1%}$)는 11.9, 분자흡광계수(分子吸光係數)(${\varepsilon}_{280,}mole/l$)는 $4.83{\times}10^4$이었다.