Optimization of Media Composition and Cultivation for the Mycelial Growth of Agrocybe Cylindracea

Agrocybe Cylindracea의 영양배지 조성 및 배양조건의 최적화

  • Lee, Jae-Sung (Department of Food Science and Technology, Yeungnam University) ;
  • Park, Shin (Department of Food Science and Technology, Yeungnam University) ;
  • Park, Gyung-Sook (Department of Food Science and Technology, Yeungnam University)
  • 이재성 (영남대학교 식품가공학과) ;
  • 박신 (영남대학교 식품가공학과) ;
  • 박경숙 (영남대학교 식품가공학과)
  • Published : 1989.06.01

Abstract

Studies were made to optimize the meida composition and cultural conditions for the mycelial growth of Agrocybe cylindracea. Media composition for optimal growth was found to be starch 20.0g/l, bacto-soytone 4.0g/l. The media supplemented with $KH_2PO_4\;0.46g/l,\;K_2HPO_4\;1.0g/l,\;MgSO_47H_2O\;0.5g/l$ supported mycelial growth better than the media without mineral salts. Optimum temperature and pH for the growth was $28^{\circ}C$, and 6.0 respectively. Temperature range for the mycelial growth appeared to be $10-35^{\circ}C$ and the mycelium evidently lost the vitality at $40^{\circ}C$.

A. cylindracea의 균사 성장을 위한 영양배지 조성 및 배양조건을 최적화하였다. 최적 영양배지 조성은 starch 20.0g/l, bacto-soytone 4.0g/l, 그리고 yeast extract 6.0g/l였다. 무기염류 $KH_2PO_4\;0.46g/l,\;K_2HPO_4\;1.0g/l,\;MgSO_47H_2O\;0.5g/l$을 첨가한 배지의 pH는 5.8로_ 완충되었으며 무기염류를 첨가하지 않은 배지보다 균사성장이 양호하였다. 균사의 최적 배양온도는 $28^{\circ}C$였으며 최적 생장 pH는 6.0이었다. 균사 성장 온도범위는 $10-35^{\circ}C$로서 $40^{\circ}C$ 이상에서는 균사가 사멸하였다.

Keywords