Abstract
The thermal resistance of the important microorganisms in takju. Korean traditional turbid alcoholic beverage, was measured and optimun heating time and temperature to achieve the commercial pasteurization of these microorganisms were examined. Most of the vegetative bacterial cells in takju were destroyed by heating at over $60^{\circ}C$, except for the spore forming organisms, which did not actively grow in takju after pasteurization. The important microorganisms for the quality deterioration of pasteurized takju were then appeared to be yeast and molds, and their thermal resistances were measured. The thermal resistances of these microorganisms changed greatly depending upon the heating method. The D values of yeast in takju were 3.5 min at $65^{\circ}C$ and 0.46 min at $80^{\circ}C$ in cap-tube, and 7.1 sec at $65^{\circ}C$ and 2.3 sec at $80^{\circ}C$ in a continuous coil heat exchanger. Those of molds were 2.7 min at 65℃ and 0.25 min at $80^{\circ}C$ in cap-tube, and 3 sec at $65^{\circ}C$ and <1 sec at $80^{\circ}C$ in the coil heat exchanger. The acidity and pH did not change at $30^{\circ}C$ for two weeks after pasteurization by heating in the coil heat exchanger at $65^{\circ}C$ for 17 sec, but the viscosity increased slightly by the heat treatment. Significant differences in sensory quality, especially the formation of burnt smell and bitterness by heating takju for 12D of yeast at $70,\;80\;and\;85^{\circ}C$, respectively, were observed and this resulted in the significant reduction in overall likeness of pasteurized takju. However, when the heating temperature was fixed to $80^{\circ}C$, the overall likeness of pasteurized takju did not affected significantly by the heating time ranging from 8D to 12D of yeast. It was concluded that the optimum pasteurization condition of takju in a continuous heat exchanger was heating at $80^{circ}C$ for 23sec(10D of yeast).
탁주의 저장성에 영향을 미치는 미생물의 열저항성을 측정하고 이들 미생물에 대한 상업적 살균조건을 수립하였다. 대부분의 탁주내 세균 영양세포 $60^{\circ}C$의 가열에서 파괴되었으며 생잔 포자형성균은 저장기간 중 거의 증식되지 않았다. 따라서 탁주의 저장 중 품질저하를 일으키는 미생물의 효모와 곰팡이류로 판단되었다. 정치형 cap-tube 가열에서 효모의 D-value는 $65^{\circ}C$에서 3.5분, $80^{\circ}C$에서 0.46분이었으며 곰팡이는 $65^{\circ}C$에서 2.7분. $80^{\circ}C$에서는 0.25분이었다. 그러나 연속식 coil형 열교환기를 사용할 경우 효모의 D-value는 $65^{\circ}C$에서 7.1초, $80^{\circ}C$에서 2.3초로, 곰팡이의 경우 $65^{\circ}C$에서 3초, $80^{\circ}C$에서 1초 이하로 크게 낮아졌다. 연속식 코일형 열교환기에서 $65^{\circ}C$ 이상의 온도에서 17초간 가열한 탁주는 $30^{\circ}C$에서 2주간 저장하는 동안 산도 및 pH변화를 보이지 않았으나 점도가 다소 증가하는 경향을 보였다. 효모의 살균효과 12D를 얻기 위한 가열온도가 $70^{\circ}C$에서 $85^{\circ}C$로 증가할 수록 화독냄새와 쓴 맛은 증가하였으며 따라서 탁주의 전체적인 기호도가 저하하였다. 그러나 $80^{\circ}C$에서 가열시간을 8D에서 12D로 증가시킬 경우 온도증가에서처럼 커다란 품질변화가 나타나지 않았다. 광범위한 품질평가시험결과 연속식 살균장치에서 $80^{\circ}C$에서 탁주효모의 10D에 해다하는 23초동안 가열살균하는 것이 탁주의 저온살균조건으로 가장 적합하다는 결론을 얻었다.